##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546252_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1240917
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.818592218496484	31.0	31.0	34.0	30.0	34.0
2	32.186577345624244	33.0	31.0	34.0	30.0	34.0
3	32.20397093439771	33.0	31.0	34.0	30.0	34.0
4	35.90996738702105	37.0	35.0	37.0	35.0	37.0
5	35.82140626649486	37.0	35.0	37.0	35.0	37.0
6	35.904768812096215	37.0	35.0	37.0	35.0	37.0
7	36.163315515864475	37.0	35.0	37.0	35.0	37.0
8	36.073711618101775	37.0	35.0	37.0	35.0	37.0
9	37.82202758121615	39.0	38.0	39.0	35.0	39.0
10	37.467473650534245	39.0	37.0	39.0	35.0	39.0
11	37.307402509595725	39.0	37.0	39.0	34.0	39.0
12	36.553076474897196	39.0	35.0	39.0	33.0	39.0
13	36.23140387310352	39.0	35.0	39.0	32.0	39.0
14	37.12476740990735	40.0	35.0	41.0	32.0	41.0
15	37.43392668486289	40.0	35.0	41.0	33.0	41.0
16	37.590474624813744	40.0	35.0	41.0	33.0	41.0
17	37.554814705576604	39.0	35.0	41.0	33.0	41.0
18	37.38622405849868	39.0	35.0	41.0	33.0	41.0
19	37.168643833552125	38.0	35.0	41.0	33.0	41.0
20	36.86986639718853	38.0	35.0	41.0	33.0	41.0
21	36.7711329605445	38.0	35.0	41.0	33.0	41.0
22	36.712889742021424	37.0	35.0	41.0	33.0	41.0
23	36.67164201957101	37.0	35.0	41.0	33.0	41.0
24	36.61202723469821	37.0	35.0	41.0	33.0	41.0
25	36.58278756758107	37.0	35.0	41.0	33.0	41.0
26	36.55411119357701	37.0	35.0	41.0	33.0	41.0
27	36.47215889539752	37.0	35.0	41.0	32.0	41.0
28	36.39216804991792	37.0	35.0	40.0	32.0	41.0
29	36.300506802630636	37.0	35.0	40.0	32.0	41.0
30	36.07807129727451	37.0	35.0	40.0	31.0	41.0
31	35.71598825707118	37.0	35.0	40.0	30.0	41.0
32	35.375984856360255	37.0	35.0	40.0	29.0	41.0
33	34.9466330141339	37.0	34.0	40.0	25.0	41.0
34	34.54357382484082	37.0	34.0	40.0	23.0	41.0
35	34.27244207307983	37.0	34.0	40.0	21.0	41.0
36	34.111689984100465	36.0	34.0	40.0	19.0	41.0
37	33.976381176178585	36.0	34.0	40.0	18.0	41.0
38	33.92664618181554	36.0	33.0	40.0	18.0	41.0
39	33.87249187496021	36.0	34.0	40.0	18.0	41.0
40	33.72143100626391	36.0	33.0	40.0	18.0	41.0
41	33.65628724564173	36.0	34.0	40.0	17.0	41.0
42	33.60736858307204	36.0	33.0	40.0	17.0	41.0
43	33.51655670766054	36.0	33.0	40.0	16.0	41.0
44	33.38252518097504	35.0	33.0	40.0	15.0	41.0
45	33.305867354545065	35.0	33.0	40.0	15.0	41.0
46	33.30550230192672	35.0	33.0	40.0	16.0	41.0
47	33.24037627012927	35.0	33.0	40.0	15.0	41.0
48	33.17329765004428	35.0	33.0	40.0	15.0	41.0
49	33.10830458443232	35.0	33.0	40.0	15.0	41.0
50	32.90480426974568	35.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	2.0
12	6.0
13	5.0
14	11.0
15	35.0
16	96.0
17	255.0
18	539.0
19	1109.0
20	1952.0
21	3144.0
22	5003.0
23	7534.0
24	11915.0
25	19581.0
26	29720.0
27	36804.0
28	35715.0
29	31264.0
30	30826.0
31	32762.0
32	39054.0
33	51935.0
34	100296.0
35	183518.0
36	65666.0
37	81410.0
38	140705.0
39	330052.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.6547222739313	22.173844020188298	25.906889824218705	19.264543881661705
2	38.44439233244448	22.37917604481202	29.299219851126225	9.87721177161728
3	26.946363052484575	23.234914180400462	39.13331834441788	10.685404422697086
4	24.027070303654476	25.54199837700668	38.52747605198414	11.903455267354706
5	20.252925860472537	29.959135058992665	38.01922288114354	11.768716199391257
6	15.794126440366277	40.01347390679634	36.111117826575025	8.081281826262353
7	78.98610463068843	3.4058684021574366	16.386430357550104	1.2215966096040267
8	78.78310958750666	3.0668449219407905	16.195281392712	1.9547640978405487
9	75.8590622902257	4.152493680076911	17.75509562686304	2.2333484028343555
10	43.96103849008435	24.203552695305167	23.322510691690095	8.512898122920388
11	38.21504580886554	19.859507122555335	29.522683628316805	12.402763440262323
12	35.0292565900862	20.365342726387016	33.99050863192301	10.614892051603773
13	20.13744674301343	34.48288644607174	33.065547494312675	12.314119316602158
14	13.450536981925461	35.96445209470093	38.33842231188709	12.246588611486505
15	11.532761659321292	22.237587203656652	54.71075019521854	11.51890094180352
16	15.438582918922055	20.133014536830423	48.52000576992659	15.908396774320927
17	15.516509162176037	20.534975344845787	35.68465900620267	28.263856486775506
18	17.19615413440222	22.752770733256135	44.415944015594924	15.635131116746729
19	21.50353327418353	23.613102246161507	36.08226819360199	18.801096286052974
20	22.43341013137865	23.44500075347505	38.10794759037067	16.01364152477563
21	19.140683865238366	25.00296151958592	40.18222008401851	15.674134531157199
22	19.419268170232176	21.864073100779503	34.49682774915647	24.21983097983185
23	14.540134432842807	26.283224421939583	36.05422441629859	23.122416728919017
24	14.135272544416749	25.273406682316384	46.21807904960606	14.37324172366081
25	15.127200288173986	24.898603210367817	42.505340808450526	17.468855693007672
26	14.592837393637126	28.115337286861248	36.40461046145713	20.887214858044494
27	13.987881542439986	31.862082637275496	37.57616343397665	16.573872386307865
28	13.046883877003863	29.817385046703365	39.882925288315015	17.252805787977763
29	14.284678185567609	24.63742538783819	39.18594071964523	21.891955706948973
30	17.11693852207682	29.600045772602034	36.95581573948943	16.32719996583172
31	21.02074514250349	29.75871875395373	33.31101113128436	15.909524972258419
32	23.162628926833946	24.749519911484814	35.03296352616654	17.054887635514703
33	22.70208241163591	29.895391875524307	31.484539256050166	15.917986456789615
34	17.704810233077637	28.091000445638187	33.45251938687277	20.751669934411407
35	17.977753548383976	27.73698804996628	33.7025764011614	20.582682000488347
36	27.11180522146123	27.767771736546443	30.21805648564731	14.902366556345026
37	16.954880946912645	33.37120854980631	34.225979658591186	15.447930844689855
38	18.068412311218236	30.34683222165544	32.14582441855499	19.43893104857134
39	17.864369655665932	30.372539017516885	33.589273094010316	18.17381823280687
40	20.035828343072097	26.532878508393388	32.61233426570834	20.81895888282617
41	14.873355752238062	26.514424413558686	35.78071700202351	22.83150283217975
42	17.84776902887139	27.04725618232323	32.641828583217084	22.463146205588284
43	17.906677078321916	25.9612044963523	32.898735370697636	23.233383054628153
44	16.439455660612275	28.578865468036945	33.44494434357818	21.53673452777261
45	17.003635214925737	35.19534344359857	28.750673896803736	19.050347444671964
46	20.44955464386417	31.176944146949392	32.08095303714914	16.292548172037293
47	17.662422224854684	30.253111207276557	32.8382961954748	19.246170372393966
48	18.376813275988642	29.348377046974132	33.797345027910815	18.477464649126414
49	21.51102773191116	26.050896232382986	34.17666129160935	18.261414744096502
50	18.704474191263394	30.407674324713096	31.0536482294948	19.834203254528706
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2738.0
1	4569.5
2	6401.0
3	20401.5
4	34402.0
5	24443.0
6	14484.0
7	15057.5
8	15631.0
9	16591.5
10	17552.0
11	17505.0
12	17458.0
13	16715.0
14	15972.0
15	14252.5
16	12533.0
17	11944.5
18	11356.0
19	10209.5
20	9063.0
21	9296.5
22	9530.0
23	11431.0
24	13332.0
25	13348.0
26	13364.0
27	16446.5
28	19529.0
29	22535.0
30	25541.0
31	30747.5
32	35954.0
33	51094.5
34	66235.0
35	66619.5
36	67004.0
37	70726.0
38	74448.0
39	77237.5
40	80027.0
41	89213.0
42	98399.0
43	99642.0
44	100885.0
45	96513.5
46	92142.0
47	86272.5
48	80403.0
49	76609.0
50	72815.0
51	63066.5
52	53318.0
53	45601.0
54	37884.0
55	34409.5
56	30935.0
57	29511.5
58	28088.0
59	25768.0
60	23448.0
61	20917.5
62	18387.0
63	16255.5
64	14124.0
65	11627.0
66	9130.0
67	8101.0
68	7072.0
69	5749.5
70	4427.0
71	3515.0
72	2603.0
73	2185.5
74	1768.0
75	1460.0
76	1152.0
77	907.5
78	663.0
79	529.0
80	395.0
81	310.0
82	225.0
83	144.0
84	63.0
85	41.0
86	19.0
87	16.0
88	13.0
89	7.5
90	2.0
91	2.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1240917.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.227332486460757
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.76952753053907	19.08375533283794
2	8.734206202966686	4.2321303536916375
3	3.3813061020165653	2.457600815161618
4	1.7652592812062884	1.710700941223819
5	1.1151446694232088	1.3508490338310224
6	0.7277847811154308	1.0579370322401762
7	0.5556688491590812	0.9423661772662248
8	0.39074793847395106	0.7573424179046018
9	0.3240922277165821	0.7066701141450648
>10	2.992337010053938	16.5344138200839
>50	0.7191443941725252	11.997722735166581
>100	0.44603035026281784	21.937328025816637
>500	0.06166009349989303	10.49322696194239
>1k	0.016755460190188323	5.027649388338862
>5k	0.0	0.0
>10k+	3.3510920380376644E-4	1.7103068503495535
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21066	1.6976155536591084	No Hit
ATGTGAGGGGCTGGCAAGGATTTTAATGATACAGTAAAGATAAACATATA	2314	0.1864750019542	No Hit
AGCGTTGGGAGTCTTATTAAAGACACGAAATTTACAAAGTTCTTAAAGAC	1763	0.1420723545571541	No Hit
GGGTATTTTAATATAAATATTTTTCTTATAATTTTCTTTCTTCTATCCAT	1711	0.13788190507503725	No Hit
TTTTTTGGGATGTAATTTAATGCAGTTAAAGTATGAAAGTAGAATGTGTG	1602	0.1290980782759846	No Hit
AAAGACGGGATTTATATTTTTGATAGTAGACTATTCTTAATATAAAGTTG	1594	0.12845339374027434	No Hit
GAGGAAGGGTCATATTCTTCCCTAGGAAGTGAAAAGAGAAAACAGTAACA	1572	0.12668051126707103	No Hit
TTGCAAGGGAGTTGAGAGATTCAAGATACAGGCAAAGTCCACAGAACAAA	1566	0.12619699786528832	No Hit
TAAGCAGAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGA	1460	0.11765492776712706	No Hit
TTAGCAGGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAAAGGAA	1459	0.11757434220016326	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1416	0.11410916282072049	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1399	0.11273920818233613	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGC	1392	0.11217510921358961	No Hit
AGATGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1354	0.10911285766896577	No Hit
TTTTTTGGGAGTGAAATTGACCTTTCAGTGAAGAGGCTGAAATATAATAA	1339	0.10790407416450898	No Hit
GGACTTGGGGATTATATTTTCTTTAATTTGTAAAATGCACCCATATTCTA	1307	0.10532533602166784	No Hit
TATGAGGGGATGGTTTACAGGTAATATTCGCATTTTAGACCAATTAAGCT	1297	0.10451948035202999	No Hit
GAGAGTGTTCCATAGCATTTTAAATATTCCCCAAAATGAATCATGTTTCA	1260	0.10153781437436991	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1250	0.10073195870473207	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09726677932528928	0.0
2	0.0	0.0	0.0	0.3575581606183169	0.0
3	0.0	0.0	0.0	0.49350601208622336	0.0
4	0.0	0.0	0.0	0.7369550098838198	0.0
5	0.0	0.0	0.0	1.2635011044251951	0.0
6	0.0	0.0	0.0	1.730897392815152	0.0
7	0.0	0.0	0.0	2.0052912483268424	0.0
8	0.0	0.0	0.0	2.6619024479477678	0.0
9	0.0	0.0	0.0	2.909219552959626	0.0
10	0.0	0.0	0.0	3.3628357093987753	0.0
11	0.0	0.0	0.0	3.9643263812164715	0.0
12	0.0	0.0	0.0	4.41367150260654	0.0
13	0.0	0.0	0.0	4.5888645251858105	0.0
14	0.0	0.0	0.0	4.660666265350543	0.0
15	0.0	0.0	0.0	4.770101465287364	0.0
16	0.0	0.0	0.0	5.044172978531199	0.0
17	0.0	0.0	0.0	5.3429842608329166	0.0
18	0.0	0.0	0.0	5.80393370386577	0.0
19	0.0	0.0	0.0	6.007895773851112	0.0
20	0.0	0.0	0.0	6.248040763403193	0.0
21	0.0	0.0	0.0	6.570785959093154	0.0
22	0.0	0.0	0.0	6.879912193966236	0.0
23	0.0	0.0	0.0	7.240371434995249	0.0
24	0.0	0.0	0.0	7.501871599792734	0.0
25	0.0	0.0	0.0	7.716873892452114	0.0
26	0.0	0.0	0.0	7.971443698490713	0.0
27	0.0	0.0	0.0	8.28193988800218	0.0
28	0.0	0.0	0.0	8.541505999192532	0.0
29	0.0	0.0	0.0	8.895760151565334	0.0
30	0.0	0.0	0.0	9.27862218021028	0.0
31	0.0	0.0	0.0	9.612407598574281	0.0
32	0.0	0.0	0.0	9.88148280666636	0.0
33	0.0	0.0	0.0	10.171026748767241	0.0
34	0.0	0.0	0.0	10.494255457858987	0.0
35	0.0	0.0	0.0	10.896538608142205	0.0
36	0.0	0.0	0.0	11.180360974988657	0.0
37	0.0	0.0	0.0	11.491340677901906	0.0
38	0.0	0.0	0.0	11.872429824073649	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTATCG	90	0.0	44.000004	37
GTATCGA	90	0.0	44.000004	38
CGTAAGG	90	0.0	44.000004	2
TGATGCG	95	0.0	44.000004	1
AACGTGT	110	0.0	44.0	35
AATCCGG	20	7.856726E-4	44.0	2
TACCCGA	20	7.856726E-4	44.0	40
ACACGAA	200	0.0	44.0	23
ATTAGGC	20	7.856726E-4	44.0	27
ATAATCG	20	7.856726E-4	44.0	1
CATTGCA	20	7.856726E-4	44.0	25
TAGCGCG	20	7.856726E-4	44.0	1
GCAAACC	20	7.856726E-4	44.0	44
GTTAGCG	155	0.0	44.0	1
ATTCGAT	30	2.5278805E-6	44.0	14
CGACATA	50	2.7284841E-11	44.0	40
GGATAGT	175	0.0	44.0	8
ATCACGT	20	7.856726E-4	44.0	17
TTATCGG	20	7.856726E-4	44.0	2
TCGATAG	25	4.4430468E-5	44.0	1
>>END_MODULE