FastQCFastQC Report
Thu 26 May 2016
SRR1546251_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546251_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1576149
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT243161.5427475448069947No Hit
TTTAGCGGGATCTTAGAAATCACTATGATTAATTTTTTTAATCTTATACA20970.1330457970661403No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC20880.1324747850615646No Hit
TGATTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT18320.1162326658203No Hit
GGTACTGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAA18050.11451962980657286No Hit
TAATTGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT16920.10735025686023339No Hit
GGGCATGGGAGATCTTTCCATCTTCTGAGATCTTCTTTGATTTCTTCCTT16450.10436830528078246No Hit
AGATGAGGGAAGGATAGCTAAGGTTTTTTTGTTGTTGTTCTTGAGAATTA16110.10221114881905202No Hit
TTAGGCGGGAAATGGTGTACATATACAGATGTTTTATGCTATATAAAGTT16070.10195736570590724No Hit
ACGTTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT16000.10151324525790391No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTACGA302.528317E-644.0000048
CGTATTC408.3164196E-944.00000430
ACGCATG551.8189894E-1244.0000041
CGCATTG207.857372E-444.0000041
CGCATGG1200.044.0000042
TATGCGT302.528317E-644.00000420
CTACGCG207.857372E-444.00000442
TTGCGAT600.044.00000411
AGCGAAT207.857372E-444.00000423
GTATCAC302.528317E-644.00000434
TCCAACG207.857372E-444.00000444
TGCTATC302.528317E-644.00000420
TTACGGC207.857372E-444.00000416
CGGTAAG207.857372E-444.00000435
ATCGCTT551.8189894E-1244.00000411
TAATGCG551.8189894E-1244.0000041
TGCGTTA302.528317E-644.00000422
TGCGTAA302.528317E-644.00000417
TGCCGGA207.857372E-444.00000418
TCGCAAG254.4435943E-544.01
Produced by FastQC (version 0.11.5)