##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546251_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1576149
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90555334552761	31.0	31.0	34.0	30.0	34.0
2	32.26134648437426	33.0	31.0	34.0	30.0	34.0
3	32.13625996019412	33.0	31.0	34.0	30.0	34.0
4	35.900304476289996	37.0	35.0	37.0	35.0	37.0
5	35.896835261133305	37.0	35.0	37.0	35.0	37.0
6	35.957207091461534	37.0	35.0	37.0	35.0	37.0
7	36.19891456962508	37.0	35.0	37.0	35.0	37.0
8	36.10932151719159	37.0	35.0	37.0	35.0	37.0
9	37.88814065167697	39.0	38.0	39.0	35.0	39.0
10	37.51296990322616	39.0	37.0	39.0	35.0	39.0
11	37.317301219618194	39.0	37.0	39.0	34.0	39.0
12	36.66351721823254	39.0	35.0	39.0	33.0	39.0
13	36.42462927045603	39.0	35.0	39.0	33.0	39.0
14	37.46554735624614	40.0	35.0	41.0	33.0	41.0
15	37.71613470553863	40.0	35.0	41.0	33.0	41.0
16	37.83452833456735	40.0	35.0	41.0	33.0	41.0
17	37.75340529353507	40.0	35.0	41.0	33.0	41.0
18	37.55917302234751	39.0	36.0	41.0	33.0	41.0
19	37.33988537885695	38.0	35.0	41.0	33.0	41.0
20	37.04513659558836	38.0	35.0	41.0	33.0	41.0
21	36.92723467134135	38.0	35.0	41.0	33.0	41.0
22	36.831380789506575	38.0	35.0	41.0	33.0	41.0
23	36.8020041252445	38.0	35.0	41.0	33.0	41.0
24	36.71040682067495	38.0	35.0	41.0	33.0	41.0
25	36.69332467932917	38.0	35.0	41.0	33.0	41.0
26	36.63178417776492	38.0	35.0	41.0	33.0	41.0
27	36.55944457027857	38.0	35.0	41.0	32.0	41.0
28	36.466243990891726	37.0	35.0	41.0	32.0	41.0
29	36.35409723319305	37.0	35.0	41.0	32.0	41.0
30	36.101761318251	37.0	35.0	41.0	31.0	41.0
31	35.7458742796525	37.0	35.0	41.0	30.0	41.0
32	35.35394813561408	37.0	35.0	41.0	28.0	41.0
33	34.927884356111	37.0	35.0	41.0	23.0	41.0
34	34.447264820775196	37.0	34.0	41.0	21.0	41.0
35	34.18662004670878	37.0	34.0	41.0	18.0	41.0
36	34.002995275192895	37.0	34.0	40.0	18.0	41.0
37	33.9409192912599	37.0	34.0	40.0	18.0	41.0
38	33.87606565115354	37.0	34.0	40.0	18.0	41.0
39	33.82268173884576	37.0	34.0	40.0	17.0	41.0
40	33.68990114513285	37.0	33.0	40.0	16.0	41.0
41	33.58858775407655	36.0	34.0	40.0	15.0	41.0
42	33.51620690683431	36.0	33.0	40.0	15.0	41.0
43	33.403871715174134	36.0	33.0	40.0	15.0	41.0
44	33.271381068668	36.0	33.0	40.0	14.0	41.0
45	33.22998269833626	36.0	33.0	40.0	14.0	41.0
46	33.180468344046155	35.0	33.0	40.0	13.0	41.0
47	33.14007114809577	35.0	33.0	40.0	13.0	41.0
48	33.06683314838889	35.0	33.0	40.0	12.0	41.0
49	32.99066776047189	36.0	33.0	40.0	12.0	41.0
50	32.8193038856098	35.0	33.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	0.0
11	1.0
12	0.0
13	7.0
14	20.0
15	39.0
16	125.0
17	305.0
18	612.0
19	1344.0
20	2531.0
21	4149.0
22	6561.0
23	10235.0
24	16077.0
25	26466.0
26	40357.0
27	48518.0
28	46405.0
29	40138.0
30	38307.0
31	40231.0
32	46565.0
33	60695.0
34	115091.0
35	223398.0
36	81157.0
37	105694.0
38	179611.0
39	441507.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.231309984018004	22.887937625186453	27.6904658125596	20.19028657823594
2	38.767464243545504	23.23282887595018	27.225471703500116	10.774235177004204
3	24.841369692839955	24.180772249324146	40.54242333687995	10.435434720955952
4	23.04134951708246	24.95734857554711	39.6022203484569	12.39908155891353
5	19.506785208758817	29.56262383822849	39.300726010040925	11.629864942971762
6	17.881938826849492	38.20615944304758	35.82104229993484	8.090859430168088
7	78.0471897009737	3.4142076669147396	17.111199512228854	1.4274031198827015
8	78.69376562748826	2.5323747945149853	16.71631298817561	2.05754658982114
9	75.54825083161553	3.718557065353593	18.398577799433937	2.3346143035969313
10	42.08948519461041	24.408161918701847	24.397376136393195	9.104976750294547
11	36.53258670341446	20.523440360016725	32.09480829540862	10.849164641160195
12	33.59840979501303	20.4174224645005	34.26991991239407	11.714247828092395
13	18.119543266531274	33.66915183780213	35.24152856106878	12.969776334597807
14	12.2962993980899	35.51593155215655	38.43272431730757	13.755044732445981
15	11.588307958194308	23.925974003726804	52.81753184502227	11.66818619305662
16	14.603568571245484	18.57584530396555	50.03372143115911	16.786864693629855
17	14.669361843328263	21.046741139321217	36.3468174645925	27.93707955275802
18	15.856178571949734	24.888192677215162	43.13069386206507	16.124934888770035
19	20.177660868356988	24.494575068727638	35.784053411193995	19.54371065172138
20	20.62476326793977	22.671777858565402	40.41933852700475	16.284120346490084
21	17.5382530458732	26.387860538565832	40.51660090511747	15.557285510443492
22	19.034938955644424	21.217283391354496	36.704207533678606	23.043570119322474
23	15.217469921942659	25.51916094227132	36.613924191177354	22.649444944608664
24	13.653277704074931	23.337197181230962	48.368523534259765	14.641001580434338
25	14.37751126321179	24.92619669840859	43.53351110840409	17.16278092997553
26	12.869278221792483	28.901011262260102	38.05756943030133	20.17214108564609
27	13.881936289018359	32.882170403940236	36.568370122367874	16.66752318467353
28	12.460750855407705	28.243268878767175	41.767497869808	17.52848239601713
29	14.103933067241739	25.13569465830959	39.242736568687356	21.51763570576132
30	15.030368321776685	31.701507915812527	36.42790116924225	16.840222593168537
31	20.46519713555	30.553520003502204	34.10197893727053	14.879303923677266
32	20.907287318648173	25.745979599644446	36.490839381302145	16.85589370040523
33	21.59992488019851	28.46932618680087	32.92296603937825	17.007782893622366
34	16.51163690742436	26.859072333897366	35.06324592408459	21.566044834593683
35	17.016157736356146	26.404039212028813	35.428820498569614	21.15098255304543
36	23.77249866605251	28.175128112887805	32.38139287592734	15.670980345132346
37	17.20554338454042	30.18267942941943	36.19575306649308	16.416024119547075
38	16.46367189904	30.532456005111193	33.46745770863034	19.536414387218468
39	16.32948407796471	30.29884864946144	34.77063399462868	18.60103327794517
40	19.023709052887767	27.504886911072497	31.598535417654038	21.8728686183857
41	14.497994796177267	27.9210912166299	34.946822920929435	22.634091066263405
42	17.050418456630688	27.05251851189196	32.98723661278217	22.909826418695186
43	18.371423006327447	25.091028830396112	33.08367419577717	23.453873967499266
44	15.731380726060799	28.1847718711873	33.536994281632005	22.546853121119895
45	16.31381297072802	34.70014573495273	29.139821171729324	19.846220122589934
46	19.577717588882777	31.329905992390312	31.800166101047555	17.292210317679356
47	16.756030045382765	30.170370948431906	33.070414028115366	20.003184978069967
48	17.288911137208473	29.326542097225577	34.401379565003054	18.983167200562892
49	19.454950007899	27.54384261894021	33.69249988421145	19.30870748894933
50	17.05200460108784	30.16250367192442	33.01445485166694	19.771036875320796
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2543.0
1	5212.5
2	7882.0
3	25184.0
4	42486.0
5	31230.5
6	19975.0
7	21124.0
8	22273.0
9	23314.5
10	24356.0
11	24251.0
12	24146.0
13	23585.0
14	23024.0
15	20206.0
16	17388.0
17	17150.0
18	16912.0
19	15649.0
20	14386.0
21	13198.0
22	12010.0
23	15325.5
24	18641.0
25	18328.0
26	18015.0
27	21345.0
28	24675.0
29	30506.0
30	36337.0
31	55563.5
32	74790.0
33	78417.0
34	82044.0
35	86082.5
36	90121.0
37	84749.0
38	79377.0
39	87904.0
40	96431.0
41	112967.0
42	129503.0
43	127102.0
44	124701.0
45	119405.5
46	114110.0
47	107365.5
48	100621.0
49	93064.5
50	85508.0
51	73902.0
52	62296.0
53	51681.0
54	41066.0
55	37796.5
56	34527.0
57	32660.0
58	30793.0
59	29026.0
60	27259.0
61	26318.5
62	25378.0
63	20915.5
64	16453.0
65	14399.0
66	12345.0
67	10005.0
68	7665.0
69	6783.0
70	5901.0
71	4748.0
72	3595.0
73	3116.0
74	2637.0
75	2228.0
76	1819.0
77	1415.5
78	1012.0
79	813.5
80	615.0
81	430.5
82	246.0
83	202.0
84	158.0
85	108.5
86	59.0
87	57.5
88	56.0
89	31.5
90	7.0
91	6.0
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1576149.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.770787479201225
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.5499293940506	18.90964465611439
2	8.453024176010038	4.0187008248896925
3	3.299446686004695	2.352913379159171
4	1.7188793282526138	1.6343646085714019
5	1.0998837744507755	1.3072551727145536
6	0.6804486088897954	0.9704879563462465
7	0.4909102899883976	0.8168526922267071
8	0.38105915605707835	0.7246460972509267
9	0.3264774835719947	0.6984564190860864
>10	2.7040928970623743	14.338211290803379
>50	0.7406390586882313	12.267376579458976
>100	0.46262956471090927	21.965050753122036
>500	0.06842838688970321	11.17771697346902
>1k	0.023882848757582686	7.267251069173315
>5k	0.0	0.0
>10k+	2.68346615253738E-4	1.5510715276140925
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24316	1.5427475448069947	No Hit
TTTAGCGGGATCTTAGAAATCACTATGATTAATTTTTTTAATCTTATACA	2097	0.1330457970661403	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	2088	0.1324747850615646	No Hit
TGATTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1832	0.1162326658203	No Hit
GGTACTGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAA	1805	0.11451962980657286	No Hit
TAATTGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1692	0.10735025686023339	No Hit
GGGCATGGGAGATCTTTCCATCTTCTGAGATCTTCTTTGATTTCTTCCTT	1645	0.10436830528078246	No Hit
AGATGAGGGAAGGATAGCTAAGGTTTTTTTGTTGTTGTTCTTGAGAATTA	1611	0.10221114881905202	No Hit
TTAGGCGGGAAATGGTGTACATATACAGATGTTTTATGCTATATAAAGTT	1607	0.10195736570590724	No Hit
ACGTTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1600	0.10151324525790391	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05595917644841954	0.0
2	0.0	0.0	0.0	0.24077672859609084	0.0
3	0.0	0.0	0.0	0.3844814164143111	0.0
4	0.0	0.0	0.0	0.6621201421946783	0.0
5	0.0	0.0	0.0	1.2050891127678918	0.0
6	0.0	0.0	0.0	1.6760471249862798	0.0
7	0.0	0.0	0.0	1.962695151283286	0.0
8	0.0	0.0	0.0	2.615615655626467	0.0
9	0.0	0.0	0.0	2.870604238558664	0.0
10	0.0	0.0	0.0	3.3276676253323765	0.0
11	0.0	0.0	0.0	3.8974741601206486	0.0
12	0.0	0.0	0.0	4.3382954276530965	0.0
13	0.0	0.0	0.0	4.506046065441782	0.0
14	0.0	0.0	0.0	4.5687304943885385	0.0
15	0.0	0.0	0.0	4.668023137406426	0.0
16	0.0	0.0	0.0	4.898140975250437	0.0
17	0.0	0.0	0.0	5.1535102328523505	0.0
18	0.0	0.0	0.0	5.587733139443035	0.0
19	0.0	0.0	0.0	5.7698859689026865	0.0
20	0.0	0.0	0.0	5.9780515674596755	0.0
21	0.0	0.0	0.0	6.249282269633137	0.0
22	0.0	0.0	0.0	6.523685260720909	0.0
23	0.0	0.0	0.0	6.836790176563256	0.0
24	0.0	0.0	0.0	7.077122784711344	0.0
25	0.0	0.0	0.0	7.2842098050374675	0.0
26	0.0	0.0	0.0	7.520164654483808	0.0
27	0.0	0.0	0.0	7.753898901690132	0.0
28	0.0	0.0	0.0	7.994865967621082	0.0
29	0.0	0.0	0.0	8.28982539087358	0.0
30	0.0	0.0	0.0	8.649689845312848	0.0
31	0.0	0.0	0.0	8.978973434618174	0.0
32	0.0	0.0	0.0	9.252044064361936	0.0
33	0.0	0.0	0.0	9.528540766133151	0.0
34	0.0	0.0	0.0	9.81296819019014	0.0
35	0.0	0.0	0.0	10.18748861941352	0.0
36	0.0	0.0	0.0	10.468680308777914	0.0
37	0.0	0.0	0.0	10.756089684414354	0.0
38	6.344577828618994E-5	0.0	0.0	11.109990235694722	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTACGA	30	2.528317E-6	44.000004	8
CGTATTC	40	8.3164196E-9	44.000004	30
ACGCATG	55	1.8189894E-12	44.000004	1
CGCATTG	20	7.857372E-4	44.000004	1
CGCATGG	120	0.0	44.000004	2
TATGCGT	30	2.528317E-6	44.000004	20
CTACGCG	20	7.857372E-4	44.000004	42
TTGCGAT	60	0.0	44.000004	11
AGCGAAT	20	7.857372E-4	44.000004	23
GTATCAC	30	2.528317E-6	44.000004	34
TCCAACG	20	7.857372E-4	44.000004	44
TGCTATC	30	2.528317E-6	44.000004	20
TTACGGC	20	7.857372E-4	44.000004	16
CGGTAAG	20	7.857372E-4	44.000004	35
ATCGCTT	55	1.8189894E-12	44.000004	11
TAATGCG	55	1.8189894E-12	44.000004	1
TGCGTTA	30	2.528317E-6	44.000004	22
TGCGTAA	30	2.528317E-6	44.000004	17
TGCCGGA	20	7.857372E-4	44.000004	18
TCGCAAG	25	4.4435943E-5	44.0	1
>>END_MODULE