FastQCFastQC Report
Thu 26 May 2016
SRR1546250_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546250_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1621002
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT250701.5465742793654789No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC21340.13164696897351144No Hit
TTTAGCGGGATCTTAGAAATCACTATGATTAATTTTTTTAATCTTATACA20900.12893259847921223No Hit
GGTACTGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAA18630.1149289143381686No Hit
TGATTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT17900.11042552692717221No Hit
TAATTGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT17300.10672411261676419No Hit
AGATGAGGGAAGGATAGCTAAGGTTTTTTTGTTGTTGTTCTTGAGAATTA17110.105551998085135No Hit
GGGCATGGGAGATCTTTCCATCTTCTGAGATCTTCTTTGATTTCTTCCTT16850.1039480518839582No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAC551.8189894E-1244.0000049
CGTACAT1100.044.00000435
TAATGCG650.044.0000041
CGATAAG1100.044.00000410
ACACGTC302.5283625E-644.011
CGATTGC502.7284841E-1144.010
TCGATAG2150.044.01
GTTACTA207.857439E-444.010
CACCTAA207.857439E-444.041
CTAACGG302.5283625E-644.02
CCGTACG207.857439E-444.037
CATAGCG207.857439E-444.01
AACGCTA254.4436507E-544.040
ACGTGTA351.4465695E-744.022
TACCTAC351.4465695E-744.015
ATGACGG3050.044.02
GCGATAT302.5283625E-644.09
CGTAAGA254.4436507E-544.019
CGTCCCA351.4465695E-744.039
TACACGG700.044.02
Produced by FastQC (version 0.11.5)