##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546250_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1621002 Sequences flagged as poor quality 0 Sequence length 50 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.84261462971668 31.0 31.0 34.0 30.0 34.0 2 32.2329663997947 33.0 31.0 34.0 30.0 34.0 3 32.234624016503375 33.0 31.0 34.0 30.0 34.0 4 35.940016113490294 37.0 35.0 37.0 35.0 37.0 5 35.86096685877007 37.0 35.0 37.0 35.0 37.0 6 35.94925731121862 37.0 35.0 37.0 35.0 37.0 7 36.18124715453775 37.0 35.0 37.0 35.0 37.0 8 36.093160896778656 37.0 35.0 37.0 35.0 37.0 9 37.86121053521217 39.0 38.0 39.0 35.0 39.0 10 37.51371867523914 39.0 37.0 39.0 35.0 39.0 11 37.31219640691375 39.0 37.0 39.0 34.0 39.0 12 36.6506352243859 39.0 35.0 39.0 33.0 39.0 13 36.37027159744405 39.0 35.0 39.0 33.0 39.0 14 37.37697856017451 40.0 35.0 41.0 33.0 41.0 15 37.66551429301136 40.0 35.0 41.0 33.0 41.0 16 37.785808407392466 40.0 35.0 41.0 33.0 41.0 17 37.71686401373965 40.0 35.0 41.0 33.0 41.0 18 37.5329314831197 39.0 36.0 41.0 33.0 41.0 19 37.323586892551646 38.0 35.0 41.0 33.0 41.0 20 37.024500895125364 38.0 35.0 41.0 33.0 41.0 21 36.90371079122666 38.0 35.0 41.0 33.0 41.0 22 36.8188953499132 38.0 35.0 41.0 33.0 41.0 23 36.78201075630999 38.0 35.0 41.0 33.0 41.0 24 36.68892203711038 37.0 35.0 41.0 33.0 41.0 25 36.664904176552525 38.0 35.0 41.0 33.0 41.0 26 36.628026985777936 38.0 35.0 41.0 33.0 41.0 27 36.546234983053694 38.0 35.0 41.0 32.0 41.0 28 36.44664534652024 37.0 35.0 41.0 32.0 41.0 29 36.34317416017994 37.0 35.0 41.0 32.0 41.0 30 36.08810599863541 37.0 35.0 40.0 31.0 41.0 31 35.74091333631914 37.0 35.0 40.0 30.0 41.0 32 35.35548383037158 37.0 35.0 41.0 28.0 41.0 33 34.91609880802121 37.0 35.0 41.0 24.0 41.0 34 34.50165576600152 37.0 34.0 41.0 21.0 41.0 35 34.22729583307115 37.0 34.0 41.0 18.0 41.0 36 34.03848360458531 37.0 34.0 40.0 18.0 41.0 37 33.91447388713894 37.0 34.0 40.0 18.0 41.0 38 33.85091134989346 37.0 33.0 40.0 18.0 41.0 39 33.80088056646444 37.0 34.0 40.0 17.0 41.0 40 33.6813236504335 37.0 33.0 40.0 16.0 41.0 41 33.599100432942095 36.0 34.0 40.0 15.0 41.0 42 33.51778838027344 36.0 33.0 40.0 15.0 41.0 43 33.43478231365538 36.0 33.0 40.0 15.0 41.0 44 33.29223159502579 36.0 33.0 40.0 14.0 41.0 45 33.25039512597763 36.0 33.0 40.0 15.0 41.0 46 33.203533370100715 36.0 33.0 40.0 14.0 41.0 47 33.14894491185082 35.0 33.0 40.0 13.0 41.0 48 33.0979351043367 35.0 33.0 40.0 12.0 41.0 49 33.019056114674754 36.0 33.0 40.0 12.0 41.0 50 32.818842296308084 35.0 33.0 40.0 10.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 3.0 11 3.0 12 4.0 13 7.0 14 26.0 15 44.0 16 94.0 17 276.0 18 662.0 19 1438.0 20 2594.0 21 4233.0 22 6657.0 23 10537.0 24 16352.0 25 26420.0 26 40708.0 27 49663.0 28 47718.0 29 41832.0 30 39848.0 31 41807.0 32 49272.0 33 63774.0 34 121639.0 35 226607.0 36 84733.0 37 109486.0 38 185170.0 39 449394.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.29040186255168 22.992322032915443 27.758633240427834 19.95864286410504 2 38.76102558787713 23.259934287557943 27.165604977661967 10.813435146902965 3 24.825015638475463 24.23877330194534 40.44041895074775 10.495792108831452 4 23.0617852414741 24.996822952716897 39.50278901568289 12.438602790126108 5 19.55802645524188 29.45690381628153 39.275460486785335 11.70960924169125 6 17.935264731320505 38.1584353381427 35.757512945696554 8.14878698484024 7 78.10329660296532 3.4070901824920634 17.041311485118463 1.4483017294241463 8 78.7218029342345 2.524796391367808 16.66833230310635 2.0850683712913374 9 75.54790185329814 3.725967025333713 18.333228459927874 2.39290266144027 10 42.32635123213913 24.231308783086018 24.32242526536056 9.119914719414288 11 36.57768466664446 20.46623014653899 31.983612605043056 10.972472581773495 12 33.71396210492029 20.302010731633892 34.25301140899271 11.731015754453109 13 18.37838571451485 33.51408573215826 35.18656978831611 12.920958765010775 14 12.32941106796907 35.44579217052169 38.512969138841285 13.711827622667954 15 11.62651249042259 23.806509800728193 52.87020003676738 11.696777672081836 16 14.799549908019854 18.586528579236795 49.9201728313722 16.69374868137115 17 14.799488217781349 20.909351129733338 36.33999217767776 27.95116847480756 18 15.9049156015847 24.790962626819706 43.12739897914993 16.17672279244566 19 20.248710365564015 24.467890847759595 35.76497746455588 19.518421322120517 20 20.568944393652817 22.716011454643485 40.37021545932701 16.34482869237669 21 17.503247991057382 26.32162082465043 40.62993136344063 15.545199820851547 22 18.96771256297031 21.25864126015884 36.68841864476416 23.085227532106682 23 15.218858459150574 25.482818651673472 36.638326171096644 22.659996718079313 24 13.629224393307348 23.469927859435092 48.2409645392171 14.659883208040458 25 14.5522954320846 24.78868008799496 43.424067336129134 17.23495714379131 26 12.83070594607533 28.779421616999855 38.09730031178247 20.29257212514235 27 13.85902053174518 32.85097735844866 36.56392774345744 16.726074366348715 28 12.43181686389036 28.18170489610747 41.70476038894462 17.681717851057556 29 14.158896781126733 25.111258345147014 39.211857850884826 21.517987022841428 30 15.065681596938191 31.627289787427777 36.36584038761211 16.94118822802193 31 20.391955099376805 30.50292350040284 34.18256115661794 14.922560243602415 32 21.002441699640098 25.68004234417971 36.43184894281438 16.885667013365808 33 21.673631494594083 28.347343186498225 32.91149548242384 17.067529836483857 34 16.619041802539417 26.82273063204117 34.86972872334519 21.68849884207422 35 16.951860639283602 26.41650041147389 35.331356778091575 21.30028217115093 36 23.839452388090823 28.116683384721302 32.26023163450755 15.78363259268033 37 17.31071275667766 30.1469708242186 36.06658104061562 16.47573537848812 38 16.483323277824457 30.46658794992233 33.41143317528294 19.63865559697027 39 16.303126091146094 30.234200821467216 34.74789050229426 18.71478258509243 40 18.99701542626104 27.419953831025502 31.596999880320936 21.986030862392518 41 14.499426897684273 27.861224107064643 34.94295503645276 22.696393958798325 42 16.96888714511148 27.007369515892023 33.156714180488365 22.86702915850813 43 18.38554178218164 25.06949405367791 33.09255633244129 23.45240783169916 44 15.762164389679963 28.077078251599936 33.49878655300857 22.66197080571153 45 16.29356410417754 34.54462116641435 29.12081539689649 20.040999332511618 46 19.563331815753465 31.268561050510733 31.74777082323156 17.420336310504243 47 16.732921982822972 30.05677969552166 33.10742367992143 20.10287464173394 48 17.2212619108428 29.310512880304895 34.41766265556736 19.050562553284944 49 19.48362802760268 27.406381978554005 33.71716999732264 19.39281999652067 50 17.07086110936322 30.023960488636042 32.93974961165995 19.965428790340788 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2583.0 1 5514.0 2 8445.0 3 26297.0 4 44149.0 5 32193.0 6 20237.0 7 21273.0 8 22309.0 9 23562.0 10 24815.0 11 24551.5 12 24288.0 13 23874.5 14 23461.0 15 20561.0 16 17661.0 17 17507.5 18 17354.0 19 15978.5 20 14603.0 21 13456.5 22 12310.0 23 15551.0 24 18792.0 25 18336.0 26 17880.0 27 21521.0 28 25162.0 29 31089.0 30 37016.0 31 56459.0 32 75902.0 33 79776.0 34 83650.0 35 87831.0 36 92012.0 37 86614.5 38 81217.0 39 89656.5 40 98096.0 41 115053.0 42 132010.0 43 130357.5 44 128705.0 45 123544.0 46 118383.0 47 112256.5 48 106130.0 49 98225.0 50 90320.0 51 78080.0 52 65840.0 53 54279.5 54 42719.0 55 39356.5 56 35994.0 57 34148.0 58 32302.0 59 30038.0 60 27774.0 61 26620.5 62 25467.0 63 21221.5 64 16976.0 65 14703.0 66 12430.0 67 10134.5 68 7839.0 69 6886.0 70 5933.0 71 4745.5 72 3558.0 73 3128.5 74 2699.0 75 2257.0 76 1815.0 77 1410.0 78 1005.0 79 808.0 80 611.0 81 444.0 82 277.0 83 215.5 84 154.0 85 109.5 86 65.0 87 53.5 88 42.0 89 24.0 90 6.0 91 5.0 92 4.0 93 2.5 94 1.0 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1621002.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 23.595279715061174 #Duplication Level Percentage of deduplicated Percentage of total 1 78.97198502090673 18.633660762219158 2 8.704522573056257 4.107712897946528 3 3.3762238724450744 2.389888399530256 4 1.7842431158671037 1.6839886159422643 5 1.0724678321111147 1.2652589242033507 6 0.7562357572692812 1.0706156533979876 7 0.5388562717091672 0.8900125120034965 8 0.430404957549325 0.8124420291300283 9 0.31391018028679357 0.6666118658344958 >10 2.7601392985904956 14.432933206098122 >50 0.732338681946014 12.062847935225038 >100 0.46591173153786614 21.949762872355922 >500 0.06904348057359753 11.290099895671919 >1k 0.023453701416222064 7.1853269139357465 >5k 0.0 0.0 >10k+ 2.6352473501373104E-4 1.5588375165057302 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 25070 1.5465742793654789 No Hit ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC 2134 0.13164696897351144 No Hit TTTAGCGGGATCTTAGAAATCACTATGATTAATTTTTTTAATCTTATACA 2090 0.12893259847921223 No Hit GGTACTGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAA 1863 0.1149289143381686 No Hit TGATTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT 1790 0.11042552692717221 No Hit TAATTGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT 1730 0.10672411261676419 No Hit AGATGAGGGAAGGATAGCTAAGGTTTTTTTGTTGTTGTTCTTGAGAATTA 1711 0.105551998085135 No Hit GGGCATGGGAGATCTTTCCATCTTCTGAGATCTTCTTTGATTTCTTCCTT 1685 0.1039480518839582 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0514496589146713 0.0 2 0.0 0.0 0.0 0.23152346511602084 0.0 3 0.0 0.0 0.0 0.3816158154030655 0.0 4 0.0 0.0 0.0 0.653978218410588 0.0 5 0.0 0.0 0.0 1.2242427831674483 0.0 6 0.0 0.0 0.0 1.7049331216124348 0.0 7 0.0 0.0 0.0 2.008819236496932 0.0 8 0.0 0.0 0.0 2.6808726947900126 0.0 9 0.0 0.0 0.0 2.946140720369253 0.0 10 0.0 0.0 0.0 3.429792190262566 0.0 11 0.0 0.0 0.0 4.043671753643734 0.0 12 0.0 0.0 0.0 4.516218980605823 0.0 13 0.0 0.0 0.0 4.695799264899118 0.0 14 0.0 0.0 0.0 4.762609793201983 0.0 15 0.0 0.0 0.0 4.869704047249788 0.0 16 0.0 0.0 0.0 5.124237971328845 0.0 17 0.0 0.0 0.0 5.401103761747364 0.0 18 0.0 0.0 0.0 5.851997714993566 0.0 19 0.0 0.0 0.0 6.057056067790169 0.0 20 0.0 0.0 0.0 6.286050233127411 0.0 21 0.0 0.0 0.0 6.591416913736072 0.0 22 0.0 0.0 0.0 6.890121048585998 0.0 23 0.0 0.0 0.0 7.235956525655119 0.0 24 0.0 0.0 0.0 7.49369834213653 0.0 25 0.0 0.0 0.0 7.715104608137436 0.0 26 0.0 0.0 0.0 7.97759657298387 0.0 27 0.0 0.0 0.0 8.23478317731872 0.0 28 0.0 0.0 0.0 8.48271624587755 0.0 29 0.0 0.0 0.0 8.793388286997795 0.0 30 0.0 0.0 0.0 9.171117617374932 0.0 31 0.0 0.0 0.0 9.53342438812537 0.0 32 0.0 0.0 0.0 9.832683735121856 0.0 33 0.0 0.0 0.0 10.120098556325038 0.0 34 0.0 0.0 0.0 10.426513970988315 0.0 35 0.0 0.0 0.0 10.815779375966223 0.0 36 0.0 0.0 0.0 11.1067722310028 0.0 37 0.0 0.0 0.0 11.407265382769422 0.0 38 0.0 0.0 0.0 11.780059494066016 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCGAC 55 1.8189894E-12 44.000004 9 CGTACAT 110 0.0 44.000004 35 TAATGCG 65 0.0 44.000004 1 CGATAAG 110 0.0 44.000004 10 ACACGTC 30 2.5283625E-6 44.0 11 CGATTGC 50 2.7284841E-11 44.0 10 TCGATAG 215 0.0 44.0 1 GTTACTA 20 7.857439E-4 44.0 10 CACCTAA 20 7.857439E-4 44.0 41 CTAACGG 30 2.5283625E-6 44.0 2 CCGTACG 20 7.857439E-4 44.0 37 CATAGCG 20 7.857439E-4 44.0 1 AACGCTA 25 4.4436507E-5 44.0 40 ACGTGTA 35 1.4465695E-7 44.0 22 TACCTAC 35 1.4465695E-7 44.0 15 ATGACGG 305 0.0 44.0 2 GCGATAT 30 2.5283625E-6 44.0 9 CGTAAGA 25 4.4436507E-5 44.0 19 CGTCCCA 35 1.4465695E-7 44.0 39 TACACGG 70 0.0 44.0 2 >>END_MODULE