FastQCFastQC Report
Thu 26 May 2016
SRR1546249_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546249_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1450503
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223401.5401553805817707No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC43010.29651782864289145No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37410.25791053172589096No Hit
ATTGTTGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA32760.22585268696445304No Hit
TAAGTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT27960.19276071817845258No Hit
GTTTAAGGGTTGGGAATGCTTCTGCAAATGTATCTTCAATATATACTCCA24790.1709062304593648No Hit
ATTTTGGGGCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCC24370.16801068319058976No Hit
ATTGATGGGGTTTCCCTCCATTAAGATTTTAACAATTATAGTTTATCTTA23960.1651840775234522No Hit
GAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCT22440.15470495407455206No Hit
GGTGAAGGGCAATCACCATCATGCTATTAATGATATTAAAATCCCAACTA21360.14725926109770196No Hit
GAAGCGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATT21330.14705243629278947No Hit
ATTTCTGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA21240.14643196187805196No Hit
GGTTAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT20970.14457053863383942No Hit
AACTTTGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC20840.14367429781255192No Hit
ATTAACGGGAAGTATAGGTAATATAACAATTAAAAAGATTTAGAGGGATA20670.1425022905847144No Hit
AATGAAGGGTATAGGTAATATAACAATTAAAAAAGATTTAGAGGGATAGA19690.13574601362423933No Hit
GTAAGCGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC19200.13236787514400175No Hit
AGGTACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT19160.13209210873745175No Hit
GATCAGAGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC19100.13167845912762677No Hit
TGCGAAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA18870.13009280228996425No Hit
ATAGACGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC18550.1278866710375642No Hit
TAGGTTGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC18440.1271283134195517No Hit
GACATAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC18430.1270593718179142No Hit
GACAGTGGGCTCTGTGGAGAAATTAAATATCTTACATTATGCTTTACAAA18350.1265078390048142No Hit
TTATTAGGGGTAAAAGATTTTGTTAAGCTCAGGATTTTTAATAACACAGT18050.12443959095568917No Hit
GTAAGAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC17980.12395699974422666No Hit
ATGTATGGGGATGGCATTCACATTAAAGCTACATGTAGATACAAGCTTCT17970.12388805814258916No Hit
GTAGAGGGCTCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCA17930.12361229173603915No Hit
ATTTAAGGGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTGCT17580.12119933567872662No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGC17300.11926897083287659No Hit
AGATAAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA17300.11926897083287659No Hit
GAACAAGGGAAGCATTTTTTCAACTAATCTTATGTTATTAACCATTTCCT17290.1192000292312391No Hit
TTGTTAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG17230.11878637962141408No Hit
GGTTATGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC17230.11878637962141408No Hit
TGAATAGGGATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATT17170.11837273001158907No Hit
GTTTTTGGGTATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTG17000.11720072278375157No Hit
CTTTTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT16830.11602871555591406No Hit
CTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGC16760.11554612434445154No Hit
TAATGAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG16730.11533929953953904No Hit
TGTCAAGGGATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA16710.11520141633626405No Hit
GTGATGGGTGTTTTTTTAAATTAAAGTTTCTGAAATCTAAAGAGACAAAG16460.11347787629532652No Hit
GCCAGCGGGGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATAT16100.11099597863637649No Hit
GGGGAAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC15590.10747995695286393No Hit
GATATAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACT15260.10520488409882642No Hit
ACTAAAGGTCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAG15120.1042397016759014No Hit
TGTCGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT14950.10306769444806388No Hit
TACTATGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA14690.10127521280548886No Hit
AATAGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT14610.10072367999238885No Hit
TAGACAGGGATGATAAAAGTTTTCACCAATACTGAGAAATCTTGATTTTT14530.10017214717928882No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGCA207.857164E-444.00000430
CGGGTAC207.857164E-444.0000046
CAGCGCA207.857164E-444.00000428
CTCACTA207.857164E-444.00000438
TCGTTCG207.857164E-444.00000427
TCGTTAC302.528177E-644.00000415
GGTACGC207.857164E-444.00000418
GCCTTAC207.857164E-444.00000423
ACCGGGT302.528177E-644.00000437
GTCGATT302.528177E-644.00000417
GGTCGTT207.857164E-444.00000425
GGTGTAA302.528177E-644.00000411
AGTCACG207.857164E-444.00000415
GATCGAC207.857164E-444.00000413
CGACGTG207.857164E-444.0000042
ACTGATC207.857164E-444.00000413
CGTGCTA207.857164E-444.00000443
CGAAACA302.528177E-644.00000443
CGAAAAG207.857164E-444.00000422
CGACGGC207.857164E-444.00000428
Produced by FastQC (version 0.11.5)