##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546249_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1450503
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.919787135910784	31.0	31.0	34.0	30.0	34.0
2	32.259101842602185	33.0	31.0	34.0	30.0	34.0
3	32.085616506825566	33.0	31.0	34.0	30.0	34.0
4	35.87887718949909	37.0	35.0	37.0	35.0	37.0
5	35.87680549436988	37.0	35.0	37.0	35.0	37.0
6	35.95708040590058	37.0	35.0	37.0	35.0	37.0
7	36.18949840158896	37.0	35.0	37.0	35.0	37.0
8	36.09799772906364	37.0	35.0	37.0	35.0	37.0
9	37.89980992800429	39.0	38.0	39.0	35.0	39.0
10	37.6023579406592	39.0	37.0	39.0	35.0	39.0
11	37.37378068159804	39.0	37.0	39.0	35.0	39.0
12	36.31068050186728	38.0	35.0	39.0	33.0	39.0
13	35.917668560492466	38.0	35.0	39.0	32.0	39.0
14	36.92948997692525	39.0	35.0	41.0	33.0	41.0
15	37.24849035127814	39.0	35.0	41.0	33.0	41.0
16	37.44772192818629	39.0	35.0	41.0	33.0	41.0
17	37.37704644526761	39.0	35.0	41.0	33.0	41.0
18	37.16968872177445	38.0	35.0	40.0	33.0	41.0
19	36.80973289955278	37.0	35.0	40.0	33.0	41.0
20	36.3956551623816	35.0	35.0	40.0	33.0	41.0
21	36.267350705238115	35.0	35.0	40.0	33.0	41.0
22	36.164964843230244	35.0	35.0	40.0	32.0	41.0
23	36.14443541309463	35.0	35.0	40.0	32.0	41.0
24	36.12348130269293	35.0	35.0	40.0	33.0	41.0
25	36.081723374581095	35.0	35.0	40.0	33.0	41.0
26	36.00120854627671	35.0	35.0	40.0	33.0	41.0
27	35.869499752844355	35.0	35.0	40.0	32.0	41.0
28	35.799344779018035	35.0	35.0	40.0	32.0	41.0
29	35.70357386368729	35.0	35.0	40.0	31.0	41.0
30	35.43067818542947	35.0	35.0	40.0	31.0	41.0
31	34.91768648530889	35.0	35.0	40.0	29.0	41.0
32	34.3558269096996	35.0	34.0	40.0	24.0	41.0
33	33.73624253103923	35.0	34.0	40.0	21.0	41.0
34	33.12655609812596	35.0	33.0	40.0	15.0	41.0
35	32.76610458578852	35.0	33.0	40.0	12.0	41.0
36	32.54710951993894	35.0	33.0	40.0	10.0	41.0
37	32.44308422664414	35.0	33.0	40.0	10.0	41.0
38	32.41627352718333	35.0	32.0	40.0	10.0	41.0
39	32.40476924211808	35.0	33.0	40.0	10.0	41.0
40	32.19495719760663	35.0	32.0	40.0	10.0	41.0
41	32.08517803823915	35.0	32.0	40.0	10.0	41.0
42	31.947671945525105	35.0	32.0	40.0	10.0	41.0
43	31.7420680963776	35.0	31.0	40.0	10.0	41.0
44	31.571483823197884	35.0	31.0	40.0	9.0	41.0
45	31.526022352246084	35.0	31.0	39.0	8.0	41.0
46	31.471486787686754	35.0	31.0	39.0	8.0	41.0
47	31.455419947425135	35.0	31.0	39.0	8.0	41.0
48	31.386293582295245	35.0	31.0	39.0	8.0	41.0
49	31.34863767948084	35.0	30.0	39.0	8.0	41.0
50	31.06532630404763	35.0	30.0	39.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	1.0
12	7.0
13	9.0
14	18.0
15	34.0
16	95.0
17	276.0
18	754.0
19	1564.0
20	2804.0
21	4534.0
22	7519.0
23	11759.0
24	18807.0
25	32503.0
26	49808.0
27	57119.0
28	51476.0
29	42140.0
30	39025.0
31	40389.0
32	47084.0
33	61132.0
34	122240.0
35	287393.0
36	62680.0
37	76187.0
38	129040.0
39	304103.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.105700574214598	22.571687200922714	27.47309036934084	22.849521855521843
2	37.9386323227184	23.501364699004416	29.303145184808304	9.256857793468887
3	22.132873906500024	22.678133033851015	44.62720863038546	10.561784429263504
4	21.520948250365564	25.108876024386024	43.03017642845275	10.339999296795662
5	16.8533260531002	29.935960146238926	43.12297182425683	10.087741976404049
6	14.585354184031333	37.66707135386828	39.83114822926943	7.916426232830956
7	73.92456272065621	4.600335194067162	20.32956843246791	1.1455336528087154
8	75.37364624547484	3.082172184407754	19.456078339720772	2.088103230396628
9	73.10863886527639	3.3911684429470332	21.205057831662533	2.295134860114043
10	49.4444341032042	16.229266675077543	25.57760997391939	8.748689247798866
11	43.82734816818717	15.565427992910047	31.719065730991247	8.888158107911531
12	39.28974983161014	17.503927947753297	34.418405201505955	8.787917019130605
13	15.973148624994227	40.95813659123766	33.04150353360179	10.027211250166323
14	9.924764030132996	43.39577374193642	37.33339400194278	9.346068225987812
15	6.974132421649594	19.87958659857994	63.78215005415363	9.364130925616838
16	9.075127731552433	15.737644113800522	60.31321548455949	14.87401267008755
17	9.145654990027598	17.785002857629387	38.92739277340343	34.14194937893958
18	13.058090882955774	22.00264322100678	48.142196189873445	16.797069706164
19	19.41919458284471	20.88330737682032	40.332698381182254	19.36479965915272
20	20.81533095760574	21.327498116170734	41.12614727442825	16.731023651795272
21	15.802380277738138	24.208636590203536	43.638999712513524	16.349983419544806
22	17.240019496684944	21.82222304952144	36.03887754799542	24.898879905798196
23	11.115799140022462	25.943689878614524	37.21839941041142	25.722111570951594
24	11.345995147890077	20.672621842216113	54.71867345327793	13.262709556615878
25	9.582744744409354	22.228013316759775	51.26959406495539	16.919647873875476
26	9.871472172067207	29.941682299174836	41.116495450199	19.070350078558956
27	10.959646412313521	35.803441978403356	37.39985370592132	15.837057903361798
28	10.073746831271635	31.111690220564864	44.382672769377244	14.431890178786254
29	10.691187815537093	23.933973249279735	41.56330597041165	23.81153296477153
30	13.165984489518465	31.03592340036525	39.55620912193908	16.241882988177206
31	19.80568120162454	30.62558298741885	35.401995032068186	14.166740778888427
32	22.921221121224843	25.210013353988238	37.792682952051806	14.076082572735112
33	23.046556953001822	27.738722360450137	34.034814129994906	15.17990655655314
34	17.660011733860596	29.667570491064133	31.679769018057875	20.992648757017392
35	15.910067059495914	25.895361815866636	34.02061216005758	24.17395896457987
36	29.292114528546303	23.555759622696403	33.236539324634286	13.915586524123011
37	16.856290541970612	31.754777480639472	37.04342562545545	14.345506351934468
38	15.472287889097784	31.51947979425068	32.60386224640693	20.4043700702446
39	17.01692447378599	30.188355349833817	35.766144571917465	17.028575604462727
40	19.95459506116154	24.359618697789664	31.858258824697366	23.827527416351433
41	13.619068695480118	25.768164560845445	34.495137204128504	26.117629539545938
42	18.142120354111643	25.03090307293401	33.97786836704233	22.84910820591202
43	18.517852083036026	22.77065266324854	34.77731517963079	23.934180074084647
44	14.653951077660645	28.312936960488877	33.635435431708856	23.397676530141613
45	14.594040825837656	36.70030327410561	29.44895667227162	19.25669922778512
46	21.526946169708026	31.26281021135427	31.410000530850336	15.800243088087374
47	17.684348119238635	29.40228320796303	32.54450352739705	20.368865145401287
48	18.83822370584549	28.748372116431337	33.49458773956345	18.918816438159727
49	22.773065619305854	23.41174061687566	34.13698558362168	19.6782081801968
50	20.02636326846618	29.553816848362256	30.0221371482858	20.397682734885763
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5066.0
1	6345.5
2	7625.0
3	22811.0
4	37997.0
5	29020.0
6	20043.0
7	22784.5
8	25526.0
9	28541.5
10	31557.0
11	31887.0
12	32217.0
13	30077.5
14	27938.0
15	25472.5
16	23007.0
17	20315.0
18	17623.0
19	16558.0
20	15493.0
21	13385.5
22	11278.0
23	10702.0
24	10126.0
25	14893.5
26	19661.0
27	19139.0
28	18617.0
29	22884.5
30	27152.0
31	29945.5
32	32739.0
33	43888.0
34	55037.0
35	57098.0
36	59159.0
37	61118.0
38	63077.0
39	80951.0
40	98825.0
41	126468.5
42	154112.0
43	154104.5
44	154097.0
45	142841.0
46	131585.0
47	117564.5
48	103544.0
49	92142.0
50	80740.0
51	66623.5
52	52507.0
53	42583.5
54	32660.0
55	28672.0
56	24684.0
57	21161.5
58	17639.0
59	17117.0
60	16595.0
61	15197.0
62	13799.0
63	11902.5
64	10006.0
65	8612.5
66	7219.0
67	5790.0
68	4361.0
69	3921.0
70	3481.0
71	2659.0
72	1837.0
73	1373.5
74	910.0
75	787.0
76	664.0
77	416.5
78	169.0
79	111.5
80	54.0
81	45.5
82	37.0
83	22.0
84	7.0
85	10.5
86	14.0
87	9.5
88	5.0
89	3.5
90	2.0
91	2.0
92	2.0
93	3.5
94	5.0
95	3.5
96	2.0
97	2.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1450503.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.8513915792353
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.12929260294577	19.350465264192174
2	8.222557901202176	3.922388965690166
3	3.1913116430600983	2.283516709499976
4	1.6300991727546046	1.5552053472943042
5	1.0186954773288897	1.214865236488368
6	0.6780837485325296	0.970394460585905
7	0.45458762133688513	0.7589783154505431
8	0.3328314936523036	0.6350795428002289
9	0.2700187284678457	0.5796290183772418
>10	1.8969230661580776	9.718787222236843
>50	0.5263311289096274	9.093724153568902
>100	0.5434780963598371	24.1699758571253
>500	0.07149497952108298	11.943853836033314
>1k	0.03400370977222239	12.254542933624181
>5k	0.0	0.0
>10k+	2.9062999805318284E-4	1.5485931370325607
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22340	1.5401553805817707	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	4301	0.29651782864289145	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3741	0.25791053172589096	No Hit
ATTGTTGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA	3276	0.22585268696445304	No Hit
TAAGTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	2796	0.19276071817845258	No Hit
GTTTAAGGGTTGGGAATGCTTCTGCAAATGTATCTTCAATATATACTCCA	2479	0.1709062304593648	No Hit
ATTTTGGGGCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCC	2437	0.16801068319058976	No Hit
ATTGATGGGGTTTCCCTCCATTAAGATTTTAACAATTATAGTTTATCTTA	2396	0.1651840775234522	No Hit
GAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCT	2244	0.15470495407455206	No Hit
GGTGAAGGGCAATCACCATCATGCTATTAATGATATTAAAATCCCAACTA	2136	0.14725926109770196	No Hit
GAAGCGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATT	2133	0.14705243629278947	No Hit
ATTTCTGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA	2124	0.14643196187805196	No Hit
GGTTAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	2097	0.14457053863383942	No Hit
AACTTTGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	2084	0.14367429781255192	No Hit
ATTAACGGGAAGTATAGGTAATATAACAATTAAAAAGATTTAGAGGGATA	2067	0.1425022905847144	No Hit
AATGAAGGGTATAGGTAATATAACAATTAAAAAAGATTTAGAGGGATAGA	1969	0.13574601362423933	No Hit
GTAAGCGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1920	0.13236787514400175	No Hit
AGGTACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1916	0.13209210873745175	No Hit
GATCAGAGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1910	0.13167845912762677	No Hit
TGCGAAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA	1887	0.13009280228996425	No Hit
ATAGACGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1855	0.1278866710375642	No Hit
TAGGTTGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1844	0.1271283134195517	No Hit
GACATAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1843	0.1270593718179142	No Hit
GACAGTGGGCTCTGTGGAGAAATTAAATATCTTACATTATGCTTTACAAA	1835	0.1265078390048142	No Hit
TTATTAGGGGTAAAAGATTTTGTTAAGCTCAGGATTTTTAATAACACAGT	1805	0.12443959095568917	No Hit
GTAAGAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1798	0.12395699974422666	No Hit
ATGTATGGGGATGGCATTCACATTAAAGCTACATGTAGATACAAGCTTCT	1797	0.12388805814258916	No Hit
GTAGAGGGCTCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCA	1793	0.12361229173603915	No Hit
ATTTAAGGGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTGCT	1758	0.12119933567872662	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGC	1730	0.11926897083287659	No Hit
AGATAAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA	1730	0.11926897083287659	No Hit
GAACAAGGGAAGCATTTTTTCAACTAATCTTATGTTATTAACCATTTCCT	1729	0.1192000292312391	No Hit
TTGTTAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG	1723	0.11878637962141408	No Hit
GGTTATGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1723	0.11878637962141408	No Hit
TGAATAGGGATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATT	1717	0.11837273001158907	No Hit
GTTTTTGGGTATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTG	1700	0.11720072278375157	No Hit
CTTTTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1683	0.11602871555591406	No Hit
CTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGC	1676	0.11554612434445154	No Hit
TAATGAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG	1673	0.11533929953953904	No Hit
TGTCAAGGGATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA	1671	0.11520141633626405	No Hit
GTGATGGGTGTTTTTTTAAATTAAAGTTTCTGAAATCTAAAGAGACAAAG	1646	0.11347787629532652	No Hit
GCCAGCGGGGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATAT	1610	0.11099597863637649	No Hit
GGGGAAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1559	0.10747995695286393	No Hit
GATATAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACT	1526	0.10520488409882642	No Hit
ACTAAAGGTCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAG	1512	0.1042397016759014	No Hit
TGTCGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1495	0.10306769444806388	No Hit
TACTATGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	1469	0.10127521280548886	No Hit
AATAGAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1461	0.10072367999238885	No Hit
TAGACAGGGATGATAAAAGTTTTCACCAATACTGAGAAATCTTGATTTTT	1453	0.10017214717928882	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13602178003078932	0.0
2	0.0	0.0	0.0	0.4492924178715935	0.0
3	0.0	0.0	0.0	0.633780143853546	0.0
4	0.0	0.0	0.0	0.9984122749142883	0.0
5	0.0	0.0	0.0	1.5879319105165588	0.0
6	0.0	0.0	0.0	2.145600526162304	0.0
7	0.0	0.0	0.0	2.4674888642077955	0.0
8	0.0	0.0	0.0	3.2459084882968186	0.0
9	0.0	0.0	0.0	3.5511819003476726	0.0
10	0.0	0.0	0.0	4.063555883717579	0.0
11	0.0	0.0	0.0	4.6994042756202505	0.0
12	0.0	0.0	0.0	5.237217709994395	0.0
13	0.0	0.0	0.0	5.421567552773072	0.0
14	0.0	0.0	0.0	5.4869931327270605	0.0
15	0.0	0.0	0.0	5.586337980686699	0.0
16	0.0	0.0	0.0	5.831218549703103	0.0
17	0.0	0.0	0.0	6.151452289309295	0.0
18	0.0	0.0	0.0	6.70415710963714	0.0
19	0.0	0.0	0.0	6.904087754385892	0.0
20	0.0	0.0	0.0	7.1429014624581955	0.0
21	0.0	0.0	0.0	7.431766773319325	0.0
22	0.0	0.0	0.0	7.677819349563565	0.0
23	6.894160163750092E-5	0.0	0.0	7.998122030771395	0.0
24	6.894160163750092E-5	0.0	0.0	8.21039322221326	0.0
25	6.894160163750092E-5	0.0	0.0	8.391020218503511	0.0
26	6.894160163750092E-5	0.0	0.0	8.610323453312402	0.0
27	6.894160163750092E-5	0.0	0.0	8.773783990794918	0.0
28	6.894160163750092E-5	0.0	0.0	8.985779415830233	0.0
29	6.894160163750092E-5	0.0	0.0	9.180746265261085	0.0
30	6.894160163750092E-5	0.0	0.0	9.526074747863328	0.0
31	6.894160163750092E-5	0.0	0.0	9.875401843360544	0.0
32	6.894160163750092E-5	0.0	0.0	10.110699529749335	0.0
33	6.894160163750092E-5	0.0	0.0	10.319385757906051	0.0
34	6.894160163750092E-5	0.0	0.0	10.527796219656215	0.0
35	6.894160163750092E-5	0.0	0.0	10.891601051497307	0.0
36	6.894160163750092E-5	0.0	0.0	11.165368151599825	0.0
37	6.894160163750092E-5	0.0	0.0	11.439066310100703	0.0
38	6.894160163750092E-5	0.0	0.0	11.674226113286219	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCA	20	7.857164E-4	44.000004	30
CGGGTAC	20	7.857164E-4	44.000004	6
CAGCGCA	20	7.857164E-4	44.000004	28
CTCACTA	20	7.857164E-4	44.000004	38
TCGTTCG	20	7.857164E-4	44.000004	27
TCGTTAC	30	2.528177E-6	44.000004	15
GGTACGC	20	7.857164E-4	44.000004	18
GCCTTAC	20	7.857164E-4	44.000004	23
ACCGGGT	30	2.528177E-6	44.000004	37
GTCGATT	30	2.528177E-6	44.000004	17
GGTCGTT	20	7.857164E-4	44.000004	25
GGTGTAA	30	2.528177E-6	44.000004	11
AGTCACG	20	7.857164E-4	44.000004	15
GATCGAC	20	7.857164E-4	44.000004	13
CGACGTG	20	7.857164E-4	44.000004	2
ACTGATC	20	7.857164E-4	44.000004	13
CGTGCTA	20	7.857164E-4	44.000004	43
CGAAACA	30	2.528177E-6	44.000004	43
CGAAAAG	20	7.857164E-4	44.000004	22
CGACGGC	20	7.857164E-4	44.000004	28
>>END_MODULE