FastQCFastQC Report
Thu 26 May 2016
SRR1546248_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546248_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1495012
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT228241.5266767089494935No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC44640.2985929209932763No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37840.25310833625415713No Hit
ATTGTTGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA34800.23277405131196272No Hit
TAAGTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT27630.18481457005027385No Hit
GTTTAAGGGTTGGGAATGCTTCTGCAAATGTATCTTCAATATATACTCCA25260.16896185448678674No Hit
ATTGATGGGGTTTCCCTCCATTAAGATTTTAACAATTATAGTTTATCTTA24880.16642006886901242No Hit
ATTTTGGGGCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCC24190.16180472129989593No Hit
GAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCT22810.15257402616166293No Hit
GAAGCGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATT22200.1484937913541831No Hit
GGTGAAGGGCAATCACCATCATGCTATTAATGATATTAAAATCCCAACTA21920.14662089668845468No Hit
AACTTTGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC20940.14006576535840515No Hit
ATTTCTGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA20880.13966443078717763No Hit
GGTTAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT20780.13899553983513177No Hit
AATGAAGGGTATAGGTAATATAACAATTAAAAAAGATTTAGAGGGATAGA20780.13899553983513177No Hit
ATTAACGGGAAGTATAGGTAATATAACAATTAAAAAGATTTAGAGGGATA20540.13739020155022166No Hit
GACAGTGGGCTCTGTGGAGAAATTAAATATCTTACATTATGCTTTACAAA19590.1310357375057859No Hit
TGCGAAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA19500.13043373564894462No Hit
GACATAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC19070.1275575045551474No Hit
GTAGAGGGCTCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCA18870.12621972265105563No Hit
GTAAGCGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC18810.12581838807982812No Hit
TTGTTAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG18650.12474816255655471No Hit
GAACAAGGGAAGCATTTTTTCAACTAATCTTATGTTATTAACCATTTCCT18640.12468127346135015No Hit
GTAAGAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC18630.12461438436614555No Hit
TAGGTTGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC18600.1244137170805318No Hit
ATAGACGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC18570.12421304979491803No Hit
ATGTATGGGGATGGCATTCACATTAAAGCTACATGTAGATACAAGCTTCT18540.12401238250930428No Hit
AGGTACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT18140.1213368187011208No Hit
GATCAGAGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC18130.1212699296059162No Hit
CTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGC17870.11953081313059695No Hit
TGAATAGGGATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATT17830.11926325674977861No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGC17750.1187281439881419No Hit
AGATAAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA17660.11812614213130063No Hit
GGTTATGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC17620.11785858575048228No Hit
ATTTAAGGGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTGCT17600.11772480756007311No Hit
TTATTAGGGGTAAAAGATTTTGTTAAGCTCAGGATTTTTAATAACACAGT17540.11732347298884557No Hit
GTTTTTGGGTATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTG17120.11451413099025291No Hit
TAATGAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG17050.11404590732382082No Hit
GTGATGGGTGTTTTTTTAAATTAAAGTTTCTGAAATCTAAAGAGACAAAG16740.11197234537247862No Hit
GATATAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACT16710.11177167808686485No Hit
CTTTTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT16130.10789211056499881No Hit
ACTAAAGGTCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAG16090.10762455418418047No Hit
GCCAGCGGGGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATAT16000.10702255232733918No Hit
TAGACAGGGATGATAAAAGTTTTCACCAATACTGAGAAATCTTGATTTTT15830.1058854377088612No Hit
TGTCAAGGGATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA15650.10468143399517864No Hit
GGGGAAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC15500.10367809756710983No Hit
GGTTTAGGGGATTCTTATTGGATTTCTGATATTTCCTATTGTTATGGGCT15010.10040053190208506No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCTAG1050.044.0000041
GACGTAG1300.044.0000041
GTCTATA650.044.00000440
GTATCAT1300.044.00000413
CCAGTGA1050.044.00000416
CGGACAG254.443485E-544.031
TCACGAC454.802132E-1044.025
AACGTAG254.443485E-544.01
ACTATCG302.5282297E-644.025
TCGTTAC254.443485E-544.015
ACACGTG454.802132E-1044.042
TCGCAAG408.314601E-944.01
AGATCGA254.443485E-544.012
ACGCCAT207.8572426E-444.033
TTCAGTA1100.044.039
GTACACG302.5282297E-644.01
GATCGAC254.443485E-544.013
CGAGTAA207.8572426E-444.021
TAGACAC207.8572426E-444.044
TGGACGC207.8572426E-444.033
Produced by FastQC (version 0.11.5)