##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546248_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1495012
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86143990817465	31.0	31.0	34.0	30.0	34.0
2	32.23783421136419	33.0	31.0	34.0	30.0	34.0
3	32.19885927337038	33.0	31.0	34.0	30.0	34.0
4	35.92517183808558	37.0	35.0	37.0	35.0	37.0
5	35.850282138203575	37.0	35.0	37.0	35.0	37.0
6	35.95027732218872	37.0	35.0	37.0	35.0	37.0
7	36.17482869702718	37.0	35.0	37.0	35.0	37.0
8	36.08400267021268	37.0	35.0	37.0	35.0	37.0
9	37.88019494157906	39.0	38.0	39.0	35.0	39.0
10	37.60245870936153	39.0	37.0	39.0	35.0	39.0
11	37.37679028663315	39.0	37.0	39.0	35.0	39.0
12	36.300806950044546	38.0	35.0	39.0	33.0	39.0
13	35.8534941525553	38.0	35.0	39.0	32.0	39.0
14	36.856841951770285	39.0	35.0	41.0	32.0	41.0
15	37.2154578023454	39.0	35.0	41.0	33.0	41.0
16	37.413422768512895	39.0	35.0	41.0	33.0	41.0
17	37.348756397941955	39.0	35.0	41.0	33.0	41.0
18	37.15180346378491	38.0	35.0	40.0	33.0	41.0
19	36.79790998333124	37.0	35.0	40.0	33.0	41.0
20	36.388907246229465	35.0	35.0	40.0	33.0	41.0
21	36.26503733749294	35.0	35.0	40.0	33.0	41.0
22	36.17008291572242	35.0	35.0	40.0	32.0	41.0
23	36.133138061768065	35.0	35.0	40.0	32.0	41.0
24	36.120635820983374	35.0	35.0	40.0	33.0	41.0
25	36.066640267770424	35.0	35.0	40.0	33.0	41.0
26	36.008215318673024	35.0	35.0	40.0	33.0	41.0
27	35.865689372393	35.0	35.0	40.0	32.0	41.0
28	35.79110736234893	35.0	35.0	40.0	32.0	41.0
29	35.69517635978842	35.0	35.0	40.0	31.0	41.0
30	35.426059456378944	35.0	35.0	40.0	31.0	41.0
31	34.92589156474998	35.0	35.0	40.0	29.0	41.0
32	34.373884624337464	35.0	34.0	40.0	24.0	41.0
33	33.74616658595382	35.0	34.0	40.0	21.0	41.0
34	33.22420823378006	35.0	34.0	40.0	16.0	41.0
35	32.8263920289603	35.0	33.0	40.0	15.0	41.0
36	32.57797730051665	35.0	33.0	40.0	12.0	41.0
37	32.40711847128986	35.0	32.0	40.0	10.0	41.0
38	32.3861895422913	35.0	32.0	40.0	10.0	41.0
39	32.37403111145596	35.0	32.0	40.0	10.0	41.0
40	32.17008492239527	35.0	32.0	40.0	10.0	41.0
41	32.09686477432957	35.0	32.0	40.0	10.0	41.0
42	31.947268650686414	35.0	32.0	40.0	10.0	41.0
43	31.764701554235018	35.0	31.0	40.0	10.0	41.0
44	31.59766610569012	35.0	31.0	40.0	9.0	41.0
45	31.544685260051423	35.0	31.0	40.0	9.0	41.0
46	31.511703584987945	35.0	31.0	39.0	8.0	41.0
47	31.467627015702885	35.0	31.0	39.0	8.0	41.0
48	31.404601434637314	35.0	31.0	39.0	8.0	41.0
49	31.359045278566327	35.0	30.0	39.0	8.0	41.0
50	31.062164718410287	35.0	30.0	39.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	5.0
14	8.0
15	33.0
16	127.0
17	326.0
18	762.0
19	1559.0
20	2879.0
21	4618.0
22	7726.0
23	12188.0
24	19367.0
25	32396.0
26	49905.0
27	58312.0
28	54084.0
29	44234.0
30	41565.0
31	41657.0
32	49559.0
33	63566.0
34	129274.0
35	291247.0
36	65156.0
37	79247.0
38	133897.0
39	311310.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.282189039285303	22.5545346793203	27.47569919171217	22.68757708968222
2	38.03133352775764	23.447704767587148	29.261771811865056	9.259189892790157
3	22.124103351678784	22.702493357912847	44.63542767549692	10.537975614911451
4	21.58497724432981	25.079932468769485	42.94299978863046	10.392090498270248
5	16.949897392127955	29.86865657265627	43.078115760943724	10.103330274272047
6	14.524498800009633	37.77976364069319	39.79359363001769	7.902143929279497
7	73.96870393013569	4.59066549298601	20.286325460932755	1.1543051159455575
8	75.38220428999901	3.0586376564201494	19.42967681864761	2.1294812349332313
9	73.08128630405642	3.384053104590465	21.195682710239115	2.3389778811139976
10	49.47779683373779	16.184151030225845	25.53404253611342	8.804009599922944
11	43.72346175147758	15.689439282092717	31.707638467115984	8.87946049931372
12	39.216273849306894	17.517585143129285	34.37430602563725	8.891834981926566
13	16.024888094543723	40.85585935096173	33.083413377283925	10.035839177210617
14	9.97068919848135	43.2863415143156	37.32692446615813	9.416044821044915
15	6.987167995975952	19.790409709085946	63.80323368641857	9.41918860851953
16	9.139993525135583	15.741813443637911	60.15316265019946	14.965030381027042
17	9.184274106161022	17.797783562941298	38.91366758260134	34.10427474829633
18	13.15300479193478	21.951395707860538	48.08764076810086	16.807958732103824
19	19.48860611152285	20.85601988479022	40.311181448710784	19.344192554976146
20	20.842909622130122	21.36885857772379	41.048366166960534	16.739865633185552
21	15.781946900760662	24.23104296152807	43.67891361407133	16.308096523639946
22	17.253707662547193	21.774139605568383	36.04091472175474	24.931238010129682
23	11.185662723777467	25.904006121689992	37.25622269252688	25.654108462005652
24	11.37729998153861	20.750402003462177	54.51401058988156	13.358287425117657
25	9.610424531709445	22.255406645565387	51.23671248123761	16.897456341487562
26	9.823198743555235	29.922970517962398	41.1411413420093	19.11268939647307
27	10.985396772734934	35.76319119846529	37.38150596784507	15.869906060954694
28	10.117778318836237	31.116071309126614	44.33355718883862	14.43259318319853
29	10.683526286076633	23.884089224701878	41.546087924377865	23.886296564843626
30	13.19527870010408	30.99058736652281	39.53613750257523	16.27799643079788
31	19.751413366581673	30.6550047758814	35.384665808702536	14.208916048834391
32	22.973996195348263	25.20922908979995	37.76344270146327	14.053332013388523
33	22.972992858920197	27.632019007205294	34.105144306533994	15.289843827340516
34	17.72353666726421	29.69247069588739	31.54382707295995	21.04016556388845
35	15.896327253560507	25.909424138401565	33.95009538384976	24.244153224188167
36	29.376285942855308	23.602352355700155	33.05358084082268	13.967780860621854
37	16.882071849590506	31.73138409591361	37.01080660222125	14.375737452274631
38	15.544223056403562	31.480416210706004	32.43719782851242	20.538162904378023
39	17.09885940714857	30.12597892190832	35.724796857817864	17.050364813125245
40	20.037096692200464	24.385623660545868	31.719812282443215	23.85746736481045
41	13.741628829735145	25.76641525285416	34.433369096702904	26.058586820707795
42	18.28995352545665	24.91632174189906	33.952637169467536	22.84108756317675
43	18.57724218936035	22.747041495319102	34.74754717687885	23.9281691384417
44	14.698410447541557	28.153887727991485	33.55023237271674	23.597469451750218
45	14.62289265905558	36.449205758883544	29.480164707708035	19.44773687435285
46	21.54210133430367	31.211522047983557	31.30817679055419	15.938199827158577
47	17.6038720759432	29.363443236576025	32.52134431027978	20.511340377200984
48	18.8272736272351	28.71294678571142	33.496520429267456	18.963259157786023
49	22.74470037698694	23.355732261680842	34.05691726889148	19.84265009244073
50	20.03749802677169	29.554210936099512	29.985846267454708	20.42244476967409
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5254.0
1	6493.0
2	7732.0
3	23240.0
4	38748.0
5	29595.5
6	20443.0
7	23268.0
8	26093.0
9	29169.0
10	32245.0
11	32424.5
12	32604.0
13	30797.0
14	28990.0
15	26401.0
16	23812.0
17	20891.5
18	17971.0
19	16935.0
20	15899.0
21	13899.0
22	11899.0
23	11052.5
24	10206.0
25	15274.0
26	20342.0
27	19729.5
28	19117.0
29	23569.5
30	28022.0
31	30657.0
32	33292.0
33	44876.5
34	56461.0
35	58317.5
36	60174.0
37	62357.5
38	64541.0
39	82855.0
40	101169.0
41	129602.5
42	158036.0
43	158427.5
44	158819.0
45	147692.5
46	136566.0
47	122417.5
48	108269.0
49	96641.0
50	85013.0
51	70025.5
52	55038.0
53	44587.5
54	34137.0
55	29882.0
56	25627.0
57	22032.0
58	18437.0
59	17765.5
60	17094.0
61	15623.5
62	14153.0
63	12106.5
64	10060.0
65	8672.0
66	7284.0
67	5807.5
68	4331.0
69	3898.5
70	3466.0
71	2620.5
72	1775.0
73	1332.0
74	889.0
75	795.5
76	702.0
77	435.0
78	168.0
79	116.5
80	65.0
81	53.0
82	41.0
83	24.5
84	8.0
85	10.0
86	12.0
87	8.0
88	4.0
89	3.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1495012.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.761715425516556
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.84613660180649	19.21042891184564
2	8.238943008691397	3.915428383591483
3	3.2603047857846277	2.324113035607921
4	1.7074132285260564	1.6228426899999446
5	1.0337937853216672	1.2282356867740505
6	0.684362046263542	0.9756969714803088
7	0.5073091573248599	0.843817508037835
8	0.3411416498912843	0.6484888643606319
9	0.2790794676801266	0.5968266202907831
>10	1.9376529358023704	9.78485184678328
>50	0.5194453870629443	8.988373533644477
>100	0.5406989624766063	24.227919123835086
>500	0.06914598891198023	11.690139320201157
>1k	0.03428960925553118	12.405933595593357
>5k	0.0	0.0
>10k+	2.8338520045893535E-4	1.5369039079540523
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22824	1.5266767089494935	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	4464	0.2985929209932763	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3784	0.25310833625415713	No Hit
ATTGTTGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA	3480	0.23277405131196272	No Hit
TAAGTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	2763	0.18481457005027385	No Hit
GTTTAAGGGTTGGGAATGCTTCTGCAAATGTATCTTCAATATATACTCCA	2526	0.16896185448678674	No Hit
ATTGATGGGGTTTCCCTCCATTAAGATTTTAACAATTATAGTTTATCTTA	2488	0.16642006886901242	No Hit
ATTTTGGGGCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCC	2419	0.16180472129989593	No Hit
GAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCT	2281	0.15257402616166293	No Hit
GAAGCGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATT	2220	0.1484937913541831	No Hit
GGTGAAGGGCAATCACCATCATGCTATTAATGATATTAAAATCCCAACTA	2192	0.14662089668845468	No Hit
AACTTTGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	2094	0.14006576535840515	No Hit
ATTTCTGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA	2088	0.13966443078717763	No Hit
GGTTAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	2078	0.13899553983513177	No Hit
AATGAAGGGTATAGGTAATATAACAATTAAAAAAGATTTAGAGGGATAGA	2078	0.13899553983513177	No Hit
ATTAACGGGAAGTATAGGTAATATAACAATTAAAAAGATTTAGAGGGATA	2054	0.13739020155022166	No Hit
GACAGTGGGCTCTGTGGAGAAATTAAATATCTTACATTATGCTTTACAAA	1959	0.1310357375057859	No Hit
TGCGAAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA	1950	0.13043373564894462	No Hit
GACATAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1907	0.1275575045551474	No Hit
GTAGAGGGCTCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCA	1887	0.12621972265105563	No Hit
GTAAGCGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1881	0.12581838807982812	No Hit
TTGTTAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG	1865	0.12474816255655471	No Hit
GAACAAGGGAAGCATTTTTTCAACTAATCTTATGTTATTAACCATTTCCT	1864	0.12468127346135015	No Hit
GTAAGAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1863	0.12461438436614555	No Hit
TAGGTTGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1860	0.1244137170805318	No Hit
ATAGACGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1857	0.12421304979491803	No Hit
ATGTATGGGGATGGCATTCACATTAAAGCTACATGTAGATACAAGCTTCT	1854	0.12401238250930428	No Hit
AGGTACGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1814	0.1213368187011208	No Hit
GATCAGAGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1813	0.1212699296059162	No Hit
CTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGCCGTCTTCTGC	1787	0.11953081313059695	No Hit
TGAATAGGGATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATT	1783	0.11926325674977861	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTACGAAGTCGTATGC	1775	0.1187281439881419	No Hit
AGATAAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA	1766	0.11812614213130063	No Hit
GGTTATGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1762	0.11785858575048228	No Hit
ATTTAAGGGGTTACCCAAAAAATGAATTTAAGTTCAATTTTAAACTTGCT	1760	0.11772480756007311	No Hit
TTATTAGGGGTAAAAGATTTTGTTAAGCTCAGGATTTTTAATAACACAGT	1754	0.11732347298884557	No Hit
GTTTTTGGGTATTGAATATACTTTTCAAGGTTTTAATAGCGTTTAAATTG	1712	0.11451413099025291	No Hit
TAATGAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG	1705	0.11404590732382082	No Hit
GTGATGGGTGTTTTTTTAAATTAAAGTTTCTGAAATCTAAAGAGACAAAG	1674	0.11197234537247862	No Hit
GATATAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACT	1671	0.11177167808686485	No Hit
CTTTTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1613	0.10789211056499881	No Hit
ACTAAAGGTCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAG	1609	0.10762455418418047	No Hit
GCCAGCGGGGCTATTAATGATATTAAAATCCCAACTATACCAAAGAATAT	1600	0.10702255232733918	No Hit
TAGACAGGGATGATAAAAGTTTTCACCAATACTGAGAAATCTTGATTTTT	1583	0.1058854377088612	No Hit
TGTCAAGGGATTAAAATCCCAACTATACCAAAGAATATCCCAATTATCCA	1565	0.10468143399517864	No Hit
GGGGAAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1550	0.10367809756710983	No Hit
GGTTTAGGGGATTCTTATTGGATTTCTGATATTTCCTATTGTTATGGGCT	1501	0.10040053190208506	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13939687440635928	0.0
2	0.0	0.0	0.0	0.4543107346295548	0.0
3	0.0	0.0	0.0	0.6343092898250984	0.0
4	0.0	0.0	0.0	1.0014635334030764	0.0
5	0.0	0.0	0.0	1.6149703146195482	0.0
6	0.0	0.0	0.0	2.185266740333857	0.0
7	0.0	0.0	0.0	2.514963090597266	0.0
8	0.0	0.0	0.0	3.313685776435239	0.0
9	0.0	0.0	0.0	3.626124740135865	0.0
10	0.0	0.0	0.0	4.18003333752505	0.0
11	0.0	0.0	0.0	4.843506272859348	0.0
12	0.0	0.0	0.0	5.403301110626537	0.0
13	0.0	0.0	0.0	5.601292832432114	0.0
14	2.0066728561376096E-4	0.0	0.0	5.667847482160679	0.0
15	2.0066728561376096E-4	0.0	0.0	5.77052224329972	0.0
16	2.0066728561376096E-4	0.0	0.0	6.041958191639933	0.0
17	2.0066728561376096E-4	0.0	0.0	6.388109259323671	0.0
18	2.0066728561376096E-4	0.0	0.0	6.967368823795394	0.0
19	2.0066728561376096E-4	0.0	0.0	7.181547706640482	0.0
20	2.0066728561376096E-4	0.0	0.0	7.442281399747962	0.0
21	2.0066728561376096E-4	0.0	0.0	7.755456143495838	0.0
22	2.0066728561376096E-4	0.0	0.0	8.02134029693407	0.0
23	2.0066728561376096E-4	0.0	0.0	8.365217135380853	0.0
24	2.0066728561376096E-4	0.0	0.0	8.591703611743585	0.0
25	2.0066728561376096E-4	0.0	0.0	8.778524854649996	0.0
26	2.0066728561376096E-4	0.0	0.0	9.00695111477366	0.0
27	2.0066728561376096E-4	0.0	0.0	9.187417893635637	0.0
28	2.0066728561376096E-4	0.0	0.0	9.40300144748002	0.0
29	2.0066728561376096E-4	0.0	0.0	9.606344296901964	0.0
30	2.0066728561376096E-4	0.0	0.0	9.961391614247912	0.0
31	2.0066728561376096E-4	0.0	0.0	10.318311826259588	0.0
32	2.0066728561376096E-4	0.0	0.0	10.575232840940407	0.0
33	2.0066728561376096E-4	0.0	0.0	10.791351507546429	0.0
34	2.0066728561376096E-4	0.0	0.0	11.017235982052318	0.0
35	2.0066728561376096E-4	0.0	0.0	11.40251717043074	0.0
36	2.0066728561376096E-4	0.0	0.0	11.685324264955733	0.0
37	2.0066728561376096E-4	0.0	0.0	11.975221603572413	0.0
38	2.0066728561376096E-4	0.0	0.0	12.22103902844927	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCTAG	105	0.0	44.000004	1
GACGTAG	130	0.0	44.000004	1
GTCTATA	65	0.0	44.000004	40
GTATCAT	130	0.0	44.000004	13
CCAGTGA	105	0.0	44.000004	16
CGGACAG	25	4.443485E-5	44.0	31
TCACGAC	45	4.802132E-10	44.0	25
AACGTAG	25	4.443485E-5	44.0	1
ACTATCG	30	2.5282297E-6	44.0	25
TCGTTAC	25	4.443485E-5	44.0	15
ACACGTG	45	4.802132E-10	44.0	42
TCGCAAG	40	8.314601E-9	44.0	1
AGATCGA	25	4.443485E-5	44.0	12
ACGCCAT	20	7.8572426E-4	44.0	33
TTCAGTA	110	0.0	44.0	39
GTACACG	30	2.5282297E-6	44.0	1
GATCGAC	25	4.443485E-5	44.0	13
CGAGTAA	20	7.8572426E-4	44.0	21
TAGACAC	20	7.8572426E-4	44.0	44
TGGACGC	20	7.8572426E-4	44.0	33
>>END_MODULE