FastQCFastQC Report
Thu 26 May 2016
SRR1546247_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546247_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences946281
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129471.3681982413257794No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC26710.28226287963089186No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA19410.20511877550114607No Hit
AGTATGGGTAAGTGTTATAGCAAAAAGTACACATACGGTTAAGTTAATGA19140.2022655004168952No Hit
AGTAGAGGGATCAATATTTTCACAACCTCAATCTTATTAATCTTCATTCT19010.2008917013022559No Hit
TTTAAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT18940.20015196331745008No Hit
GGATTAGGGACTGACATAACACACATAATTAAAATAACAAAAATGAAATA16520.17457816441416452No Hit
GGTTGAGGGATCCATAATAATCTAAAATGGTTGGTCTAATGTTGCCTTGC15500.16379912520699452No Hit
AAGTGGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT14910.15756419076363154No Hit
GGATGAGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA14640.15471091567938064No Hit
GATGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA14590.15418253140451937No Hit
GTTGATGGGTTATCATTTGGTTTTTAATATTCGATAAATCCATAAATAAA13750.14530567558685No Hit
AGAACGGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA13610.14382619961723844No Hit
ATTTTAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG13580.14350916905232167No Hit
GTGACAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT13290.14044454025812628No Hit
GAATCTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT13220.1397048022733205No Hit
AAGAATGGGCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAAT13220.1397048022733205No Hit
TATCGAGGGATAAAAGAAAACTCTTCCAATACTTCTATGCCTCAGCAAAG13160.13907074114348697No Hit
TCGTGAGGGACCATCATGCTATTAATGATATTAAAATCCCAACTATACCA13000.13737991146393091No Hit
GACTGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT12830.13558340492940257No Hit
ATAGTGGGAAGGAACCACAAGTATATGTATACAAATACATATATGAAGTA12620.13336419097498523No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA12620.13336419097498523No Hit
GAGTTAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC12580.13294148355509622No Hit
TTAGATGGGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAA12540.13251877613520718No Hit
GGGAAAGGGTTAAAGGTTGTTGTCTCTGTTAATGTCCCAGAGGTTCCAAT12530.13241309928023495No Hit
GGAGAGGGATATAAATACTGTTCAACTCTCCTCTGGTAACAAGAGTTGAT12310.13008820847084532No Hit
GACTTTGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC12290.12987685476090083No Hit
GGAGAAGGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAAT12270.1296655010509563No Hit
GATTAGGGGGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATG12060.12744628709653896No Hit
TAGACTGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC12040.12723493338659447No Hit
GGAGGAGGGAAAAGAGATAAAGTAGAAGATATTTCACTGTTATGGTGGTT12040.12723493338659447No Hit
TGCATAGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA11650.12311353604267654No Hit
GATGTAGGGTTCGATAAATCCATAAATAAAAATATATCAACAATAATTTT11620.12279650547775978No Hit
TATGCGGGTCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC11590.122479474912843No Hit
AAATACGGGATGTTTTTACTTATAAATGTAATGGATTTATAAATGACTTT11440.1208943220882592No Hit
GAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCT11300.11941484611864762No Hit
GTATAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA11290.11930916926367538No Hit
GGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATA11130.11761833958411931No Hit
CTCTGGGGGCTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATG11040.11666724788936902No Hit
GTAATAGGGAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAA10900.11518777191975745No Hit
TTGCATGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT10880.11497641820981296No Hit
GACCTTGGGAGTGCTAAGAATTTTTTTTTTTTAAAGCTAGCTACATTGCA10820.11434235707997942No Hit
TTCAGCGGGGAACTTTTAACACCAGAAGGAAAAATATTTTTGAAGAAAAT10690.11296855796534011No Hit
AGAAATGGGAGAGAGAAATACAATAAAAATATACAAACGGGTGAACATTT10540.11138340514075629No Hit
TTATAAGGGGGGAATTCTACATTAATTAGGGCTGAAAGCCCTAACTTAAT10530.11127772828578404No Hit
AGCTTAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC10320.1090585143313667No Hit
GAGTAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT10120.1069449772319216No Hit
GTGCCTGGTCCTTTTCATTTTCCCATACACAGTTAGTTTGTTTGTTTGTT10080.10652226981203258No Hit
ACTCACGGGTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC10050.10620523924711582No Hit
AATTCTGGGGGGAATTCAATACCTTTACAAATGCCTTAACAAGAGGAAAT10020.10588820868219904No Hit
TTAGATGGGAACAACATCCACACTACTACTCCTTGTCATATTTCTTCCTG9970.10535982440733778No Hit
GTTTCTGGGCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATT9870.10430305585761522No Hit
TGTAATGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA9750.10303493359794817No Hit
TTGTTAGGGGGGAATTCACATCTGTATAAAAAACACTACAGGGTGGTAAC9680.1022951956131424No Hit
TTTTTTGGGTAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACA9620.10166113448330887No Hit
ATTTAAGGGAATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCAC9520.10060436593358633No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCATT302.5272402E-644.036
GTCATGT207.8557763E-444.034
AGCGTTG207.8557763E-444.01
TATCAGT207.8557763E-444.039
CGGGTGC502.7284841E-1144.06
TAGAGCG551.8189894E-1244.01
AGTCCTC1000.044.038
CTCACGA454.802132E-1044.024
GGTACGA302.5272402E-644.07
CGAACTC454.802132E-1044.013
CGAACGG408.309144E-944.02
ACGCCAT207.8557763E-444.027
GTACAGG1000.044.02
TGCCATA207.8557763E-444.034
CACGGGT1250.044.04
CACGGAG302.5272402E-644.031
GCGAATG207.8557763E-444.01
CCGATAT302.5272402E-644.011
ACCATAC207.8557763E-444.028
GCGAACG408.309144E-944.01
Produced by FastQC (version 0.11.5)