##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546247_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 946281 Sequences flagged as poor quality 0 Sequence length 50 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.9843873014464 33.0 31.0 34.0 30.0 34.0 2 32.33436896651206 33.0 31.0 34.0 31.0 34.0 3 32.19116414680206 33.0 31.0 34.0 30.0 34.0 4 35.94579517077908 37.0 35.0 37.0 35.0 37.0 5 35.94736024500122 37.0 35.0 37.0 35.0 37.0 6 36.02252396486879 37.0 35.0 37.0 35.0 37.0 7 36.254908425721325 37.0 36.0 37.0 35.0 37.0 8 36.16114769291574 37.0 37.0 37.0 35.0 37.0 9 37.93725119705457 39.0 39.0 39.0 35.0 39.0 10 37.61886268455142 39.0 37.0 39.0 35.0 39.0 11 37.41135244182225 39.0 37.0 39.0 35.0 39.0 12 36.42797646787793 38.0 35.0 39.0 33.0 39.0 13 36.08061136174138 38.0 35.0 39.0 33.0 39.0 14 37.169300662276854 39.0 35.0 41.0 33.0 41.0 15 37.43397362939761 39.0 35.0 41.0 33.0 41.0 16 37.597390204389605 39.0 35.0 41.0 34.0 41.0 17 37.521145410295674 39.0 35.0 41.0 34.0 41.0 18 37.350452983838835 39.0 35.0 41.0 34.0 41.0 19 37.04355999961956 37.0 35.0 40.0 34.0 41.0 20 36.661164072828264 36.0 35.0 40.0 33.0 41.0 21 36.5497954624472 35.0 35.0 40.0 33.0 41.0 22 36.412896380673395 35.0 35.0 40.0 33.0 41.0 23 36.439618886990225 35.0 35.0 40.0 33.0 41.0 24 36.39721816246971 35.0 35.0 40.0 33.0 41.0 25 36.34446427646756 35.0 35.0 40.0 33.0 41.0 26 36.30807022438367 35.0 35.0 40.0 33.0 41.0 27 36.23501370100425 35.0 35.0 40.0 33.0 41.0 28 36.16590737846369 36.0 35.0 40.0 32.0 41.0 29 36.07630714343836 36.0 35.0 40.0 32.0 41.0 30 35.86445146843274 36.0 35.0 40.0 32.0 41.0 31 35.44426761184046 35.0 35.0 40.0 30.0 41.0 32 34.95832104839894 35.0 35.0 40.0 27.0 41.0 33 34.42857037180288 35.0 34.0 40.0 23.0 41.0 34 33.88180783509338 35.0 34.0 40.0 18.0 41.0 35 33.552912929668885 35.0 34.0 40.0 17.0 41.0 36 33.39943209258138 35.0 33.0 40.0 16.0 41.0 37 33.34731543801471 35.0 33.0 40.0 16.0 41.0 38 33.351499184703066 35.0 33.0 40.0 16.0 41.0 39 33.3345887743704 35.0 33.0 40.0 15.0 41.0 40 33.11569079374942 35.0 33.0 40.0 15.0 41.0 41 33.02142492557707 35.0 33.0 40.0 14.0 41.0 42 32.904110935335275 35.0 33.0 40.0 12.0 41.0 43 32.737971067790646 35.0 33.0 40.0 10.0 41.0 44 32.56685382037682 35.0 33.0 40.0 10.0 41.0 45 32.51156051954969 35.0 33.0 40.0 10.0 41.0 46 32.473986056995756 35.0 33.0 40.0 10.0 41.0 47 32.458409288572845 35.0 33.0 40.0 10.0 41.0 48 32.438600162108294 35.0 33.0 40.0 10.0 41.0 49 32.412232730024165 35.0 33.0 40.0 10.0 41.0 50 32.17078753562631 35.0 32.0 40.0 10.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 1.0 11 7.0 12 4.0 13 6.0 14 10.0 15 16.0 16 48.0 17 145.0 18 359.0 19 763.0 20 1383.0 21 2368.0 22 3694.0 23 5976.0 24 9620.0 25 16951.0 26 26728.0 27 32442.0 28 30091.0 29 25355.0 30 23527.0 31 23632.0 32 28355.0 33 37652.0 34 78073.0 35 183160.0 36 43029.0 37 52765.0 38 92829.0 39 227290.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.115241667115793 22.242019019720356 27.136759588325244 21.505979724838607 2 37.98586255034181 23.974379703280526 28.315901936105657 9.723855810272001 3 24.2304347228783 23.846616385619072 41.48651404815272 10.436434843349913 4 20.96047579947183 26.03782597346877 42.01997081205266 10.981727415006748 5 18.57947058009196 29.485850397503487 40.62662147924348 11.30805754316107 6 17.02665487312965 38.71027739117662 36.91070622785409 7.352361507839637 7 77.53426307830338 3.525485558729384 17.55303128774645 1.3872200752207855 8 78.28287791892683 2.7971606742606054 17.06543827890447 1.8545231279080947 9 74.95342292617097 3.8247624120108084 19.10014044454026 2.1216742172779544 10 46.42986597004484 19.919347424285174 24.303774460229043 9.347012145440942 11 43.567819706831266 16.146472348065743 30.184163055160145 10.10154488994284 12 38.01207041037493 18.521136956147274 33.04747744063338 10.419315192844408 13 14.840412097463652 41.78219788836508 32.61303988984245 10.76435012432882 14 10.487371087446542 41.996721903958765 36.70949749598692 10.806409512607777 15 7.574705610701262 19.341400704441917 61.6815723870605 11.402321297796322 16 10.12172916924254 17.35319635499392 56.66012526934389 15.864949206419658 17 9.507429611288824 17.983770148613363 38.64042499003995 33.86837525005786 18 14.964899432620966 22.263999805554587 46.49665374238731 16.274447019437144 19 21.73212819447923 21.70592033444611 38.25470446939123 18.307247001683432 20 22.694738666421497 19.64067755772334 39.299848565066824 18.36473521078834 21 16.82227583561331 25.764228595945603 40.00344506547209 17.41005050296899 22 16.680985880515404 23.408691498614047 35.03969751057033 24.870625110300217 23 12.092602514475088 25.331270521124278 36.151312348023474 26.424814616377162 24 11.78994400183455 22.713337792896613 52.15543797244159 13.341280232827247 25 11.180082871789669 24.360734285059088 47.912934952725465 16.546247890425782 26 11.105792042744174 30.189235544198816 39.89311842888107 18.811853984175947 27 12.449156223151475 35.73557960056262 37.024203170094296 14.791061006191608 28 10.946114314881097 29.152017212646136 43.93240485648555 15.969463615987218 29 10.77935623773488 25.812522918667923 42.937245913211825 20.47087493038537 30 14.082497693602642 30.745624185627733 38.55789136630662 16.613986754463 31 20.898126455038195 29.18636219051212 34.48552808309582 15.429983271353858 32 22.60301115630558 24.345622494798057 38.15452281087753 14.896843538018834 33 24.273445202852006 28.083095824601784 31.86949753825766 15.773961434288546 34 17.19436404197062 26.807576185086674 32.84320407997202 23.154855692970695 35 15.935752699251069 25.920736018159513 36.43632282588364 21.70718845670578 36 28.181163946016035 24.118522933462682 32.52268617884117 15.177626941680114 37 15.137998121065518 32.05041631397017 37.9870249957465 14.824560569217812 38 15.483878467389708 30.181521133785843 34.02002153694305 20.314578861881408 39 16.98301033202611 32.5655909819599 33.71989926882184 16.731499417192143 40 20.205414670695067 22.96907578192947 32.78846346909639 24.037046078279072 41 12.934847048603956 26.605416361524746 34.0784608377427 26.381275752128598 42 18.013359668005595 24.95453253314819 33.43013333248791 23.601974466358303 43 19.43534742851225 21.033498506257654 34.47538310501849 25.05577096021161 44 14.087992890061196 28.885077476986222 35.5151376810905 21.51179195186208 45 15.551300300862007 36.958472166301554 28.51869582079742 18.971531712039024 46 21.376102870077705 30.191454758153235 32.82819796656596 15.604244405203104 47 17.583571898833434 28.46363817935687 32.84521194021649 21.107577981593206 48 17.721374517717255 29.119363064459712 34.02086695178282 19.138395466040215 49 19.83808192281151 25.140312444189412 35.93224422766599 19.08936140533309 50 18.722345688014446 31.254458242319146 30.75439536459043 19.268800705075975 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2671.0 1 3201.0 2 3731.0 3 12933.0 4 22135.0 5 17195.0 6 12255.0 7 13714.0 8 15173.0 9 16938.0 10 18703.0 11 18317.5 12 17932.0 13 16635.0 14 15338.0 15 13661.0 16 11984.0 17 10913.5 18 9843.0 19 8668.0 20 7493.0 21 8533.0 22 9573.0 23 8599.5 24 7626.0 25 9190.5 26 10755.0 27 13035.5 28 15316.0 29 16836.0 30 18356.0 31 21159.5 32 23963.0 33 32960.0 34 41957.0 35 42793.0 36 43629.0 37 46168.0 38 48707.0 39 57437.0 40 66167.0 41 79619.0 42 93071.0 43 96442.5 44 99814.0 45 91366.5 46 82919.0 47 73661.5 48 64404.0 49 56686.5 50 48969.0 51 42136.0 52 35303.0 53 29696.0 54 24089.0 55 20592.5 56 17096.0 57 15558.0 58 14020.0 59 13044.5 60 12069.0 61 11099.5 62 10130.0 63 8613.5 64 7097.0 65 6149.0 66 5201.0 67 4060.5 68 2920.0 69 2707.5 70 2495.0 71 1941.0 72 1387.0 73 1245.5 74 1104.0 75 826.0 76 548.0 77 328.5 78 109.0 79 117.0 80 125.0 81 99.5 82 74.0 83 46.5 84 19.0 85 14.0 86 9.0 87 4.5 88 0.0 89 0.0 90 0.0 91 0.5 92 1.0 93 1.0 94 1.0 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 946281.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 22.43406221969561 #Duplication Level Percentage of deduplicated Percentage of total 1 80.3429257393511 18.024181949489865 2 8.198553466792319 3.6785371717104 3 3.07327907015253 2.0683840163487033 4 1.556282341067141 1.3965493948365513 5 0.9409406485372125 1.0554560527162282 6 0.625891253383181 0.842476999269693 7 0.45819918634310364 0.7195488338804562 8 0.36931019619261185 0.6628102335802437 9 0.26852979826368095 0.5421792781880755 >10 2.6226578711494235 13.500886631663466 >50 0.8853419944160383 14.027328870887438 >100 0.5657666755757008 24.618775759419417 >500 0.06864951293596369 10.680134682758998 >1k 0.02319880092318773 6.8076113566517025 >5k 0.0 0.0 >10k+ 4.7344491679974956E-4 1.3751387685987704 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12947 1.3681982413257794 No Hit ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC 2671 0.28226287963089186 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1941 0.20511877550114607 No Hit AGTATGGGTAAGTGTTATAGCAAAAAGTACACATACGGTTAAGTTAATGA 1914 0.2022655004168952 No Hit AGTAGAGGGATCAATATTTTCACAACCTCAATCTTATTAATCTTCATTCT 1901 0.2008917013022559 No Hit TTTAAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT 1894 0.20015196331745008 No Hit GGATTAGGGACTGACATAACACACATAATTAAAATAACAAAAATGAAATA 1652 0.17457816441416452 No Hit GGTTGAGGGATCCATAATAATCTAAAATGGTTGGTCTAATGTTGCCTTGC 1550 0.16379912520699452 No Hit AAGTGGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT 1491 0.15756419076363154 No Hit GGATGAGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA 1464 0.15471091567938064 No Hit GATGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA 1459 0.15418253140451937 No Hit GTTGATGGGTTATCATTTGGTTTTTAATATTCGATAAATCCATAAATAAA 1375 0.14530567558685 No Hit AGAACGGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA 1361 0.14382619961723844 No Hit ATTTTAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG 1358 0.14350916905232167 No Hit GTGACAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT 1329 0.14044454025812628 No Hit GAATCTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT 1322 0.1397048022733205 No Hit AAGAATGGGCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAAT 1322 0.1397048022733205 No Hit TATCGAGGGATAAAAGAAAACTCTTCCAATACTTCTATGCCTCAGCAAAG 1316 0.13907074114348697 No Hit TCGTGAGGGACCATCATGCTATTAATGATATTAAAATCCCAACTATACCA 1300 0.13737991146393091 No Hit GACTGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT 1283 0.13558340492940257 No Hit ATAGTGGGAAGGAACCACAAGTATATGTATACAAATACATATATGAAGTA 1262 0.13336419097498523 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA 1262 0.13336419097498523 No Hit GAGTTAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC 1258 0.13294148355509622 No Hit TTAGATGGGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAA 1254 0.13251877613520718 No Hit GGGAAAGGGTTAAAGGTTGTTGTCTCTGTTAATGTCCCAGAGGTTCCAAT 1253 0.13241309928023495 No Hit GGAGAGGGATATAAATACTGTTCAACTCTCCTCTGGTAACAAGAGTTGAT 1231 0.13008820847084532 No Hit GACTTTGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC 1229 0.12987685476090083 No Hit GGAGAAGGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAAT 1227 0.1296655010509563 No Hit GATTAGGGGGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATG 1206 0.12744628709653896 No Hit TAGACTGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC 1204 0.12723493338659447 No Hit GGAGGAGGGAAAAGAGATAAAGTAGAAGATATTTCACTGTTATGGTGGTT 1204 0.12723493338659447 No Hit TGCATAGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA 1165 0.12311353604267654 No Hit GATGTAGGGTTCGATAAATCCATAAATAAAAATATATCAACAATAATTTT 1162 0.12279650547775978 No Hit TATGCGGGTCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC 1159 0.122479474912843 No Hit AAATACGGGATGTTTTTACTTATAAATGTAATGGATTTATAAATGACTTT 1144 0.1208943220882592 No Hit GAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCT 1130 0.11941484611864762 No Hit GTATAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA 1129 0.11930916926367538 No Hit GGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATA 1113 0.11761833958411931 No Hit CTCTGGGGGCTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATG 1104 0.11666724788936902 No Hit GTAATAGGGAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAA 1090 0.11518777191975745 No Hit TTGCATGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT 1088 0.11497641820981296 No Hit GACCTTGGGAGTGCTAAGAATTTTTTTTTTTTAAAGCTAGCTACATTGCA 1082 0.11434235707997942 No Hit TTCAGCGGGGAACTTTTAACACCAGAAGGAAAAATATTTTTGAAGAAAAT 1069 0.11296855796534011 No Hit AGAAATGGGAGAGAGAAATACAATAAAAATATACAAACGGGTGAACATTT 1054 0.11138340514075629 No Hit TTATAAGGGGGGAATTCTACATTAATTAGGGCTGAAAGCCCTAACTTAAT 1053 0.11127772828578404 No Hit AGCTTAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC 1032 0.1090585143313667 No Hit GAGTAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT 1012 0.1069449772319216 No Hit GTGCCTGGTCCTTTTCATTTTCCCATACACAGTTAGTTTGTTTGTTTGTT 1008 0.10652226981203258 No Hit ACTCACGGGTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC 1005 0.10620523924711582 No Hit AATTCTGGGGGGAATTCAATACCTTTACAAATGCCTTAACAAGAGGAAAT 1002 0.10588820868219904 No Hit TTAGATGGGAACAACATCCACACTACTACTCCTTGTCATATTTCTTCCTG 997 0.10535982440733778 No Hit GTTTCTGGGCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATT 987 0.10430305585761522 No Hit TGTAATGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA 975 0.10303493359794817 No Hit TTGTTAGGGGGGAATTCACATCTGTATAAAAAACACTACAGGGTGGTAAC 968 0.1022951956131424 No Hit TTTTTTGGGTAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACA 962 0.10166113448330887 No Hit ATTTAAGGGAATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCAC 952 0.10060436593358633 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.10472576327750426 0.0 2 0.0 0.0 0.0 0.3695519618379741 0.0 3 0.0 0.0 0.0 0.5115816549206842 0.0 4 0.0 0.0 0.0 0.7589711724107321 0.0 5 0.0 0.0 0.0 1.2166576312955666 0.0 6 0.0 0.0 0.0 1.6388366669097234 0.0 7 0.0 0.0 0.0 1.8868602455296049 0.0 8 0.0 0.0 0.0 2.4953475764598463 0.0 9 0.0 0.0 0.0 2.751719626622536 0.0 10 0.0 0.0 0.0 3.1742156928016096 0.0 11 0.0 0.0 0.0 3.6541999680855897 0.0 12 0.0 0.0 0.0 4.029141449527149 0.0 13 0.0 0.0 0.0 4.170220050915109 0.0 14 0.0 0.0 0.0 4.213230530888817 0.0 15 0.0 0.0 0.0 4.299251490836231 0.0 16 0.0 0.0 0.0 4.508068956261407 0.0 17 0.0 0.0 0.0 4.762855853599512 0.0 18 0.0 0.0 0.0 5.203211308268897 0.0 19 0.0 0.0 0.0 5.363523097261807 0.0 20 0.0 0.0 0.0 5.583859339878957 0.0 21 1.0567685497225454E-4 0.0 0.0 5.845726586500205 0.0 22 1.0567685497225454E-4 0.0 0.0 6.058348418704381 0.0 23 1.0567685497225454E-4 0.0 0.0 6.3495938310079145 0.0 24 1.0567685497225454E-4 0.0 0.0 6.54065758479775 0.0 25 1.0567685497225454E-4 0.0 0.0 6.6952628236221585 0.0 26 1.0567685497225454E-4 0.0 0.0 6.885903869992106 0.0 27 1.0567685497225454E-4 0.0 0.0 7.054035746252963 0.0 28 1.0567685497225454E-4 0.0 0.0 7.256195569814886 0.0 29 1.0567685497225454E-4 0.0 0.0 7.435846223267719 0.0 30 1.0567685497225454E-4 0.0 0.0 7.706273295141718 0.0 31 1.0567685497225454E-4 0.0 0.0 8.01009425318695 0.0 32 2.1135370994450909E-4 0.0 0.0 8.229268050399405 0.0 33 2.1135370994450909E-4 0.0 0.0 8.42677809234255 0.0 34 2.1135370994450909E-4 0.0 0.0 8.635806911477669 0.0 35 2.1135370994450909E-4 0.0 0.0 8.946074157676208 0.0 36 2.1135370994450909E-4 0.0 0.0 9.173596426431473 0.0 37 2.1135370994450909E-4 0.0 0.0 9.409150136164628 0.0 38 2.1135370994450909E-4 0.0 0.0 9.619447077559414 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGCATT 30 2.5272402E-6 44.0 36 GTCATGT 20 7.8557763E-4 44.0 34 AGCGTTG 20 7.8557763E-4 44.0 1 TATCAGT 20 7.8557763E-4 44.0 39 CGGGTGC 50 2.7284841E-11 44.0 6 TAGAGCG 55 1.8189894E-12 44.0 1 AGTCCTC 100 0.0 44.0 38 CTCACGA 45 4.802132E-10 44.0 24 GGTACGA 30 2.5272402E-6 44.0 7 CGAACTC 45 4.802132E-10 44.0 13 CGAACGG 40 8.309144E-9 44.0 2 ACGCCAT 20 7.8557763E-4 44.0 27 GTACAGG 100 0.0 44.0 2 TGCCATA 20 7.8557763E-4 44.0 34 CACGGGT 125 0.0 44.0 4 CACGGAG 30 2.5272402E-6 44.0 31 GCGAATG 20 7.8557763E-4 44.0 1 CCGATAT 30 2.5272402E-6 44.0 11 ACCATAC 20 7.8557763E-4 44.0 28 GCGAACG 40 8.309144E-9 44.0 1 >>END_MODULE