FastQCFastQC Report
Thu 26 May 2016
SRR1546246_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546246_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences969448
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133261.3745966776969987No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC26670.27510500821085815No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20570.2121826028832903No Hit
AGTAGAGGGATCAATATTTTCACAACCTCAATCTTATTAATCTTCATTCT18460.19041763972900042No Hit
AGTATGGGTAAGTGTTATAGCAAAAAGTACACATACGGTTAAGTTAATGA18220.18794200410955514No Hit
TTTAAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT17690.18247497544994679No Hit
GGATTAGGGACTGACATAACACACATAATTAAAATAACAAAAATGAAATA17050.1758732804647593No Hit
GGTTGAGGGATCCATAATAATCTAAAATGGTTGGTCTAATGTTGCCTTGC16080.16586758650283462No Hit
GGATGAGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA15330.15813122519206807No Hit
AAGTGGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT15310.15792492222378096No Hit
GATGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA14410.1486412886508611No Hit
GAATCTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT14320.14771292529356914No Hit
ATTTTAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG13940.14379316889611407No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA13860.14296795702296566No Hit
GTTGATGGGTTATCATTTGGTTTTTAATATTCGATAAATCCATAAATAAA13850.14286480553882208No Hit
GTGACAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT13750.14183329069738657No Hit
GACTGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT13640.14069862437180747No Hit
GAGTTAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC13230.13646941352192177No Hit
TATCGAGGGATAAAAGAAAACTCTTCCAATACTTCTATGCCTCAGCAAAG13220.13636626203777819No Hit
GGGAAAGGGTTAAAGGTTGTTGTCTCTGTTAATGTCCCAGAGGTTCCAAT13180.135953656101204No Hit
AAGAATGGGCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAAT13140.13554105016462978No Hit
TCGTGAGGGACCATCATGCTATTAATGATATTAAAATCCCAACTATACCA13080.13492214125976842No Hit
GACTTTGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC13060.13471583829148134No Hit
GGAGAAGGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAAT12850.1325496571244667No Hit
ATAGTGGGAAGGAACCACAAGTATATGTATACAAATACATATATGAAGTA12560.12955826408430363No Hit
AGAACGGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA12540.12935196111601655No Hit
GATTAGGGGGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATG12270.12656687104414058No Hit
GACCTTGGGAGTGCTAAGAATTTTTTTTTTTTAAAGCTAGCTACATTGCA12250.12636056807585347No Hit
TTAGATGGGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAA12240.12625741659170994No Hit
TGCATAGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA12010.12388493245640818No Hit
GTATAGGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA12000.12378178097226464No Hit
GGAGGAGGGAAAAGAGATAAAGTAGAAGATATTTCACTGTTATGGTGGTT12000.12378178097226464No Hit
GGAGAGGGATATAAATACTGTTCAACTCTCCTCTGGTAACAAGAGTTGAT11930.12305972058325974No Hit
AAATACGGGATGTTTTTACTTATAAATGTAATGGATTTATAAATGACTTT11900.1227502661308291No Hit
GGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATGATA11790.12161559980525No Hit
TATGCGGGTCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCAC11510.11872735824923047No Hit
GATGTAGGGTTCGATAAATCCATAAATAAAAATATATCAACAATAATTTT11380.11738638895536428No Hit
GAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCT11320.11676748005050297No Hit
TAGACTGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC11220.11573596520906744No Hit
CTCTGGGGGCTCCTATGGTTTTCAAAACAATCACCATCATGCTATTAATG11090.11439499591520123No Hit
GTAATAGGGAAATCCCAACTATACCAAAGAATATCCCAATTATCCATAAA10950.11295087513719147No Hit
AGCTTAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC10760.11099099693846395No Hit
TTATAAGGGGGGAATTCTACATTAATTAGGGCTGAAAGCCCTAACTTAAT10750.1108878454543204No Hit
GTGCCTGGTCCTTTTCATTTTCCCATACACAGTTAGTTTGTTTGTTTGTT10730.11068154248603328No Hit
GAGTAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT10710.11047523951774618No Hit
TTGTTAGGGGGGAATTCACATCTGTATAAAAAACACTACAGGGTGGTAAC10620.1095468761604542No Hit
TTGCATGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT10440.10769014944587021No Hit
AATTCTGGGGGGAATTCAATACCTTTACAAATGCCTTAACAAGAGGAAAT10390.10717439202515246No Hit
TTCAGCGGGGAACTTTTAACACCAGAAGGAAAAATATTTTTGAAGAAAAT10310.10634918015200402No Hit
GTTTCTGGGCAATTATCCATAAAACTGTAACTAAGTGAGGCTCTCTCATT10250.10573027124714271No Hit
TTAGATGGGAACAACATCCACACTACTACTCCTTGTCATATTTCTTCCTG10030.10346093859598451No Hit
ATTTAAGGGAATAACGTCTCTTTCAGAAGGCATTGGTATCTTTTCCCCAC10030.10346093859598451No Hit
ACTCACGGGTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC10020.10335778711184096No Hit
AGAAATGGGAGAGAGAAATACAATAAAAATATACAAACGGGTGAACATTT9970.10284202969112319No Hit
TTTTTTGGGTAACCCCAGCACTTAGATATTTTAAAGAGGCATCTATCACA9940.10253257523869254No Hit
TGTAATGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA9730.10036639407167791No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGC9710.10016009110339079No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAC207.85587E-444.0000046
AGCGTAA207.85587E-444.00000418
CTCACGA800.044.00000424
TCGTTAG408.310963E-944.0000042
TCCATAC207.85587E-444.00000434
CGAACTC207.85587E-444.00000413
CGAACAC207.85587E-444.00000411
ACTGCAT207.85587E-444.00000422
GGTCGAC207.85587E-444.0000048
CGACGGT800.044.00000428
CTATATG408.310963E-944.0000041
ACACCAT207.85587E-444.00000439
CGTTGAC207.85587E-444.00000413
ATTACCA207.85587E-444.00000411
AGCCAAA207.85587E-444.00000429
TTCGTTA408.310963E-944.0000041
TATATCG207.85587E-444.00000439
CACGACG800.044.00000426
CTGACTA207.85587E-444.00000422
AAGCGAT207.85587E-444.00000415
Produced by FastQC (version 0.11.5)