FastQCFastQC Report
Thu 26 May 2016
SRR1546245_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546245_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1163075
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167021.436020892891688No Hit
GATATGGGGTAGGGAAGATTTTTATGGCTTTCTGAATTGGAATTTCATAG20530.1765148421211014No Hit
GATGCTGGGAACAAAAGCAATTGTAAATATATTATTAAGATGATTTATTA16850.1448745781656385No Hit
GCTTAGGGGGAACTACATTTTGATTATTCTCTCATCTATTAGAAAACATG14950.12853857231906798No Hit
TCCTTAGGGAGAACTTCAAAGAACATCTAATACCAATACTCCTCTAACTA14680.12621713990929218No Hit
TGTGAAGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTA13500.11607162048879048No Hit
GGTAGAGGGATAGGAAGAAAGATAAATAGATAATAGGTAAATAGACGATA13410.1152978096855319No Hit
AGATAAGGGGGCTTTACCTACTTTTTAAAATTAAGTCTTTGATTTTATGA13290.11426606194785374No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12180.10472239537433096No Hit
AAATAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT11990.10308879478967392No Hit
GGACTGGGGCAGTCAGGTGTATATTCCAAAACTCTTTTAGAAAATGCAGT11900.10231498398641531No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTA454.802132E-1044.00000413
GACAATC207.856519E-444.0000049
CGTTATA207.856519E-444.00000436
ATCGTAC207.856519E-444.00000441
CATTACG800.044.00000416
CCGTATT408.312782E-944.00000436
CCCGTAT408.312782E-944.00000435
GTTAACG408.312782E-944.0000041
TGCGATA207.856519E-444.00000415
ACTCGAT207.856519E-444.00000442
ACGTAGT207.856519E-444.00000435
CGATATC408.312782E-944.00000410
TGCACGT207.856519E-444.00000443
GACTACG207.856519E-444.0000049
AGCACGG408.312782E-944.0000042
CGAACTC351.4460966E-744.013
ACGTTCA302.5277423E-644.026
TAACGAG551.8189894E-1244.01
CCGTAAT302.5277423E-644.035
TGACCGT254.442874E-544.032
Produced by FastQC (version 0.11.5)