##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546245_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1163075
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87957698342755	31.0	31.0	34.0	30.0	34.0
2	32.22460202480494	33.0	31.0	34.0	30.0	34.0
3	32.13325108011091	33.0	31.0	34.0	30.0	34.0
4	35.890382821400166	37.0	35.0	37.0	35.0	37.0
5	35.888586720546826	37.0	35.0	37.0	35.0	37.0
6	35.9506540850762	37.0	35.0	37.0	35.0	37.0
7	36.19389549255207	37.0	35.0	37.0	35.0	37.0
8	36.088634868774584	37.0	35.0	37.0	35.0	37.0
9	37.83308557057799	39.0	38.0	39.0	35.0	39.0
10	37.431660898910216	39.0	37.0	39.0	34.0	39.0
11	37.28063882380758	39.0	37.0	39.0	34.0	39.0
12	36.76696773638845	39.0	35.0	39.0	33.0	39.0
13	36.59456784816113	39.0	35.0	39.0	33.0	39.0
14	37.6691425746405	40.0	35.0	41.0	33.0	41.0
15	37.882146895084155	40.0	35.0	41.0	33.0	41.0
16	37.95873438944178	40.0	35.0	41.0	33.0	41.0
17	37.8916200588956	40.0	35.0	41.0	33.0	41.0
18	37.69259334092814	39.0	36.0	41.0	33.0	41.0
19	37.49564903381123	39.0	36.0	41.0	33.0	41.0
20	37.22584356124928	38.0	35.0	41.0	33.0	41.0
21	37.10849257356576	38.0	35.0	41.0	33.0	41.0
22	37.02210261591041	38.0	35.0	41.0	33.0	41.0
23	37.00940266104937	38.0	35.0	41.0	33.0	41.0
24	36.94045439889947	38.0	35.0	41.0	33.0	41.0
25	36.926592008253984	38.0	35.0	41.0	33.0	41.0
26	36.84955914278959	38.0	35.0	41.0	33.0	41.0
27	36.804958407669325	38.0	35.0	41.0	33.0	41.0
28	36.72769683812308	38.0	35.0	41.0	32.0	41.0
29	36.6419551619629	38.0	35.0	41.0	32.0	41.0
30	36.424233174988714	38.0	35.0	41.0	31.0	41.0
31	36.123708273327175	38.0	35.0	41.0	31.0	41.0
32	35.78045611847903	38.0	35.0	41.0	29.0	41.0
33	35.387978419276486	38.0	35.0	41.0	26.0	41.0
34	34.916973539969476	38.0	35.0	41.0	23.0	41.0
35	34.68927283279238	38.0	34.0	41.0	21.0	41.0
36	34.51926917868581	38.0	34.0	41.0	21.0	41.0
37	34.46478172086925	38.0	34.0	41.0	20.0	41.0
38	34.412711132128194	38.0	34.0	40.0	20.0	41.0
39	34.333312125185394	38.0	34.0	40.0	18.0	41.0
40	34.16808546310427	38.0	34.0	40.0	18.0	41.0
41	34.05327171506567	37.0	34.0	40.0	18.0	41.0
42	34.0294013713647	37.0	34.0	40.0	18.0	41.0
43	33.92425595941792	37.0	34.0	40.0	18.0	41.0
44	33.7599398147153	37.0	33.0	40.0	17.0	41.0
45	33.72728413902801	37.0	33.0	40.0	17.0	41.0
46	33.65773832298003	37.0	33.0	40.0	17.0	41.0
47	33.6193530081895	37.0	33.0	40.0	17.0	41.0
48	33.554321088493865	36.0	33.0	40.0	17.0	41.0
49	33.50146809105174	36.0	33.0	40.0	17.0	41.0
50	33.34902564323023	36.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	0.0
11	2.0
12	2.0
13	6.0
14	8.0
15	28.0
16	66.0
17	218.0
18	432.0
19	853.0
20	1543.0
21	2585.0
22	4204.0
23	6274.0
24	9890.0
25	16561.0
26	26109.0
27	32389.0
28	31940.0
29	28349.0
30	27770.0
31	29626.0
32	34790.0
33	44604.0
34	81112.0
35	145702.0
36	66582.0
37	84671.0
38	142258.0
39	344499.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.859854265632055	22.7363669582787	26.32813877007072	19.075640006018528
2	37.654665434301315	23.410356167916945	27.24527652988844	11.6897018678933
3	26.919760118650988	22.978483760720504	39.40700298776949	10.694753132859015
4	23.812909743567698	25.614255314575583	37.902628807256626	12.67020613460009
5	19.743094813318145	30.058594673602308	37.81071727962513	12.387593233454421
6	18.236829095286204	40.047202458998775	33.9731315693313	7.742836876383724
7	79.03144681125465	3.3002170969198033	15.962857081443588	1.7054790103819617
8	79.07073920426456	2.852008683876792	15.794940137136471	2.2823119747221803
9	75.19824602884594	4.38785117038884	17.609268533843476	2.8046342669217377
10	39.459966038303634	26.594501644347957	24.176772779055522	9.768759538292887
11	32.53556305483309	22.501730326935064	32.63572856436601	12.32697805386583
12	30.577477806676267	21.043010983814458	35.61412634610838	12.7653848634009
13	19.232895557036304	32.059067557982075	35.23934398039679	13.468692904584827
14	13.598951056466694	32.621628011951074	39.14321948283644	14.636201448745782
15	13.529909937020399	23.938439051651873	49.3423897856974	13.189261225630332
16	14.47215355845496	21.82215248371773	46.33725254175354	17.36844141607377
17	16.37555617651484	21.30240956086237	36.75979622982181	25.562238032800984
18	16.79229628355867	24.51415428927627	41.61554499924768	17.078004427917374
19	20.971820389914665	25.537734024031124	36.401349869956796	17.089095716097415
20	21.549083249145585	24.328611654450487	37.661199836639945	16.46110525976399
21	17.9172452335404	26.15437525525009	39.74894138383166	16.179438127377857
22	18.772650087053716	23.308471078821228	35.50372933817682	22.415149495948242
23	15.574747974120328	26.50078455817553	36.88171442082411	21.04275304688004
24	14.562947359370634	24.79255422049309	45.55037293381768	15.094125486318596
25	15.49556133525353	25.36715173140167	41.75483094383423	17.382455989510564
26	14.05051264965716	29.276615867420414	36.88592739075296	19.786944092169463
27	14.702749177826021	31.20718784257249	36.120198611439505	17.969864368161982
28	13.040001719579564	28.009027792704682	41.264062936612	17.686907551103754
29	13.879156546224447	25.308256131375877	39.797691464436944	21.014895857962728
30	16.02751327300475	30.002020505986287	37.22434064871139	16.746125572297572
31	19.146744620940183	30.290479977645468	34.15016228532124	16.412613116093116
32	20.60563592201707	25.743481718719774	35.99578703007115	17.655095329192015
33	21.1637254691228	27.6355351116652	32.6963437439546	18.5043956752574
34	17.054274229950778	27.731229714334845	33.21892397308858	21.9955720826258
35	17.201126324613632	27.37734024031124	35.683253444532816	19.738279990542313
36	23.505105001827054	28.37512628162414	31.71558154031339	16.40418717623541
37	16.237301979665972	31.69915955548868	35.28336521720439	16.78017324764095
38	16.976807170646776	30.49287449218666	33.479956150721144	19.050362186445412
39	17.88844227586355	30.032543043225928	32.440212368076004	19.63880231283451
40	19.411215957698342	27.4340003869054	33.31109343765449	19.843690217741763
41	14.5374116028631	28.42103905595082	34.51007028781463	22.53147905337145
42	18.000300926423492	28.067751434774195	31.262214388581995	22.66973325022032
43	18.048105238269244	26.29907787545945	33.20490939965178	22.447907486619524
44	15.825548653354254	29.01489585796273	32.89650280506416	22.263052683618856
45	16.63736216495067	33.08367904047461	30.752015132300155	19.526943662274572
46	19.982116372546912	31.189562152053824	31.38731380177547	17.441007673623798
47	17.07035229886293	30.926810394858457	32.00490080175397	19.997936504524645
48	17.495088450873762	29.076972680179697	33.59611375018808	19.831825118758463
49	19.043139952281667	27.570964899082174	34.269329149023065	19.116565999613094
50	17.77391827698128	29.78371988048922	33.15813683554371	19.284225006985793
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1912.0
1	4424.5
2	6937.0
3	18864.5
4	30792.0
5	22621.5
6	14451.0
7	14503.5
8	14556.0
9	15035.5
10	15515.0
11	15356.0
12	15197.0
13	14371.0
14	13545.0
15	12617.5
16	11690.0
17	10832.0
18	9974.0
19	11593.5
20	13213.0
21	11898.0
22	10583.0
23	13463.0
24	16343.0
25	14417.0
26	12491.0
27	16754.5
28	21018.0
29	23559.0
30	26100.0
31	34384.0
32	42668.0
33	52117.0
34	61566.0
35	66753.0
36	71940.0
37	72066.5
38	72193.0
39	70514.0
40	68835.0
41	79976.0
42	91117.0
43	90108.5
44	89100.0
45	85577.0
46	82054.0
47	78128.0
48	74202.0
49	70581.5
50	66961.0
51	56955.0
52	46949.0
53	40084.0
54	33219.0
55	28981.0
56	24743.0
57	23109.0
58	21475.0
59	20714.0
60	19953.0
61	18498.0
62	17043.0
63	15355.0
64	13667.0
65	11836.0
66	10005.0
67	8528.5
68	7052.0
69	6147.0
70	5242.0
71	4348.0
72	3454.0
73	2898.5
74	2343.0
75	1935.0
76	1527.0
77	1111.0
78	695.0
79	516.0
80	337.0
81	262.0
82	187.0
83	123.5
84	60.0
85	96.5
86	133.0
87	81.5
88	30.0
89	16.5
90	3.0
91	2.0
92	1.0
93	2.5
94	4.0
95	2.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1163075.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.64917761153002
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.22401536766304	20.063816636522436
2	8.835200036489145	4.532312299386131
3	3.322473132977461	2.5565611049182655
4	1.8204163214230997	1.8676872622043685
5	1.0882327806036785	1.3956137936196467
6	0.7514372966281226	1.1564249211085615
7	0.5341680866694603	0.9590680490577306
8	0.4264525652042897	0.8750526070253932
9	0.3619145747478587	0.8354530087118268
>10	3.542089144617714	20.654476375693914
>50	0.6208568133533048	10.889611570334427
>100	0.41525714175740464	21.832303477347818
>500	0.04970912038952588	8.586027737152493
>1k	0.007439460194350813	2.3469505065016296
>5k	0.0	0.0
>10k+	3.3815728156140056E-4	1.4486406504153966
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16702	1.436020892891688	No Hit
GATATGGGGTAGGGAAGATTTTTATGGCTTTCTGAATTGGAATTTCATAG	2053	0.1765148421211014	No Hit
GATGCTGGGAACAAAAGCAATTGTAAATATATTATTAAGATGATTTATTA	1685	0.1448745781656385	No Hit
GCTTAGGGGGAACTACATTTTGATTATTCTCTCATCTATTAGAAAACATG	1495	0.12853857231906798	No Hit
TCCTTAGGGAGAACTTCAAAGAACATCTAATACCAATACTCCTCTAACTA	1468	0.12621713990929218	No Hit
TGTGAAGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTA	1350	0.11607162048879048	No Hit
GGTAGAGGGATAGGAAGAAAGATAAATAGATAATAGGTAAATAGACGATA	1341	0.1152978096855319	No Hit
AGATAAGGGGGCTTTACCTACTTTTTAAAATTAAGTCTTTGATTTTATGA	1329	0.11426606194785374	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1218	0.10472239537433096	No Hit
AAATAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1199	0.10308879478967392	No Hit
GGACTGGGGCAGTCAGGTGTATATTCCAAAACTCTTTTAGAAAATGCAGT	1190	0.10231498398641531	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08778453668078155	0.0
2	0.0	0.0	0.0	0.34563549212217615	0.0
3	0.0	0.0	0.0	0.4978182834297014	0.0
4	0.0	0.0	0.0	0.7480171098166498	0.0
5	0.0	0.0	0.0	1.3116093115233325	0.0
6	0.0	0.0	0.0	1.7838058594673603	0.0
7	0.0	0.0	0.0	2.064871139006513	0.0
8	0.0	0.0	0.0	2.739634159448015	0.0
9	0.0	0.0	0.0	2.9730670850976937	0.0
10	0.0	0.0	0.0	3.413107495217419	0.0
11	0.0	0.0	0.0	3.9874470691915826	0.0
12	0.0	0.0	0.0	4.435827440190874	0.0
13	0.0	0.0	0.0	4.602368720847752	0.0
14	0.0	0.0	0.0	4.668572534015433	0.0
15	0.0	0.0	0.0	4.7813769533349095	0.0
16	0.0	0.0	0.0	5.054532166885196	0.0
17	0.0	0.0	0.0	5.372052533155643	0.0
18	0.0	0.0	0.0	5.821206714958192	0.0
19	0.0	0.0	0.0	6.030823463663134	0.0
20	0.0	0.0	0.0	6.26038733529652	0.0
21	0.0	0.0	0.0	6.6023257313586825	0.0
22	0.0	0.0	0.0	6.93601014551942	0.0
23	0.0	0.0	0.0	7.334436730219461	0.0
24	0.0	0.0	0.0	7.642929303785224	0.0
25	0.0	0.0	0.0	7.88410033746749	0.0
26	0.0	0.0	0.0	8.159576983427552	0.0
27	0.0	0.0	0.0	8.443995443114158	0.0
28	0.0	0.0	0.0	8.715774993014207	0.0
29	0.0	0.0	0.0	9.055649893601014	0.0
30	0.0	0.0	0.0	9.435505019022848	0.0
31	0.0	0.0	0.0	9.809255636996754	0.0
32	0.0	0.0	0.0	10.123164886185327	0.0
33	0.0	0.0	0.0	10.45435590998001	0.0
34	0.0	0.0	0.0	10.770844528512779	0.0
35	0.0	0.0	0.0	11.17477376781377	0.0
36	0.0	0.0	0.0	11.48472798400791	0.0
37	0.0	0.0	0.0	11.796057863852289	0.0
38	0.0	0.0	0.0	12.149603421963329	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGTA	45	4.802132E-10	44.000004	13
GACAATC	20	7.856519E-4	44.000004	9
CGTTATA	20	7.856519E-4	44.000004	36
ATCGTAC	20	7.856519E-4	44.000004	41
CATTACG	80	0.0	44.000004	16
CCGTATT	40	8.312782E-9	44.000004	36
CCCGTAT	40	8.312782E-9	44.000004	35
GTTAACG	40	8.312782E-9	44.000004	1
TGCGATA	20	7.856519E-4	44.000004	15
ACTCGAT	20	7.856519E-4	44.000004	42
ACGTAGT	20	7.856519E-4	44.000004	35
CGATATC	40	8.312782E-9	44.000004	10
TGCACGT	20	7.856519E-4	44.000004	43
GACTACG	20	7.856519E-4	44.000004	9
AGCACGG	40	8.312782E-9	44.000004	2
CGAACTC	35	1.4460966E-7	44.0	13
ACGTTCA	30	2.5277423E-6	44.0	26
TAACGAG	55	1.8189894E-12	44.0	1
CCGTAAT	30	2.5277423E-6	44.0	35
TGACCGT	25	4.442874E-5	44.0	32
>>END_MODULE