FastQCFastQC Report
Thu 26 May 2016
SRR1546244_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546244_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1190860
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171771.4424029692827034No Hit
GATATGGGGTAGGGAAGATTTTTATGGCTTTCTGAATTGGAATTTCATAG21560.1810456308886015No Hit
GATGCTGGGAACAAAAGCAATTGTAAATATATTATTAAGATGATTTATTA17740.14896797272559326No Hit
TCCTTAGGGAGAACTTCAAAGAACATCTAATACCAATACTCCTCTAACTA14960.12562349898392758No Hit
GCTTAGGGGGAACTACATTTTGATTATTCTCTCATCTATTAGAAAACATG14380.12075306921048655No Hit
TGTGAAGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTA13810.11596661236417379No Hit
GGTAGAGGGATAGGAAGAAAGATAAATAGATAATAGGTAAATAGACGATA13760.11554674772853231No Hit
AGATAAGGGGGCTTTACCTACTTTTTAAAATTAAGTCTTTGATTTTATGA12760.10714945501570294No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12280.10311875451354482No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTGC408.312782E-944.024
CTAGACG254.4429376E-544.01
TCGTTGA750.044.024
ACTGCCG207.8565965E-444.021
CGTATTA302.527795E-644.037
GTATTCG351.4461511E-744.016
CGTATAG207.8565965E-444.017
ACGTTGT302.527795E-644.026
TAGACGA1700.044.042
GTTTACG207.8565965E-444.01
CGTTGAT750.044.025
ACTCTCG207.8565965E-444.043
CGACAGG454.802132E-1044.02
TAACGCG207.8565965E-444.01
GTTACGA207.8565965E-444.023
CCGTATT302.527795E-644.036
ATATCGG207.8565965E-444.02
GTTAACG254.4429376E-544.01
CGTAGGT1050.044.035
GTATGCG454.802132E-1044.01
Produced by FastQC (version 0.11.5)