##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1546244_1.fastq.gz File type Conventional base calls Encoding Illumina 1.5 Total Sequences 1190860 Sequences flagged as poor quality 0 Sequence length 50 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.814205700082294 31.0 31.0 34.0 30.0 34.0 2 32.19484322254505 33.0 31.0 34.0 30.0 34.0 3 32.22631879482055 33.0 31.0 34.0 30.0 34.0 4 35.926742018373275 37.0 35.0 37.0 35.0 37.0 5 35.85283156710277 37.0 35.0 37.0 35.0 37.0 6 35.93661051676939 37.0 35.0 37.0 35.0 37.0 7 36.16955309608182 37.0 35.0 37.0 35.0 37.0 8 36.07067413465899 37.0 35.0 37.0 35.0 37.0 9 37.812044236938014 39.0 38.0 39.0 35.0 39.0 10 37.431950019313774 39.0 37.0 39.0 34.0 39.0 11 37.270595200107486 39.0 37.0 39.0 34.0 39.0 12 36.74895705624507 39.0 35.0 39.0 33.0 39.0 13 36.533595048956215 39.0 35.0 39.0 33.0 39.0 14 37.58600675142334 40.0 35.0 41.0 33.0 41.0 15 37.83982332096132 40.0 35.0 41.0 33.0 41.0 16 37.91368254874629 40.0 35.0 41.0 33.0 41.0 17 37.857629780158874 40.0 35.0 41.0 33.0 41.0 18 37.66807265337655 39.0 36.0 41.0 33.0 41.0 19 37.474084275229664 39.0 36.0 41.0 33.0 41.0 20 37.19771173773575 38.0 35.0 41.0 33.0 41.0 21 37.08754429571906 38.0 35.0 41.0 33.0 41.0 22 37.01375476546362 38.0 35.0 41.0 33.0 41.0 23 36.985140150815376 38.0 35.0 41.0 33.0 41.0 24 36.91699024234587 38.0 35.0 41.0 33.0 41.0 25 36.89717934937776 38.0 35.0 41.0 33.0 41.0 26 36.84102413381925 38.0 35.0 41.0 33.0 41.0 27 36.78664746485733 38.0 35.0 41.0 33.0 41.0 28 36.69844566111885 38.0 35.0 41.0 32.0 41.0 29 36.61541574996221 38.0 35.0 41.0 32.0 41.0 30 36.40234368439615 38.0 35.0 41.0 31.0 41.0 31 36.11121878306434 38.0 35.0 41.0 31.0 41.0 32 35.778499571738074 38.0 35.0 41.0 29.0 41.0 33 35.374607426565674 38.0 35.0 41.0 26.0 41.0 34 34.97971465999362 38.0 35.0 41.0 23.0 41.0 35 34.72408091631258 38.0 34.0 41.0 22.0 41.0 36 34.54625564717935 38.0 34.0 41.0 21.0 41.0 37 34.42331928186353 38.0 34.0 40.0 20.0 41.0 38 34.38180222696203 38.0 34.0 40.0 20.0 41.0 39 34.30767680499807 38.0 34.0 40.0 18.0 41.0 40 34.15223871823724 38.0 34.0 40.0 18.0 41.0 41 34.07344104260786 37.0 34.0 40.0 18.0 41.0 42 34.03750146952623 37.0 34.0 40.0 18.0 41.0 43 33.95310195992812 37.0 34.0 40.0 18.0 41.0 44 33.79924676284366 37.0 34.0 40.0 17.0 41.0 45 33.75598894916279 37.0 33.0 40.0 18.0 41.0 46 33.6998362527921 37.0 33.0 40.0 18.0 41.0 47 33.64229380447744 37.0 33.0 40.0 18.0 41.0 48 33.585394588784574 36.0 33.0 40.0 17.0 41.0 49 33.52176494298238 36.0 33.0 40.0 17.0 41.0 50 33.34485749794266 36.0 33.0 40.0 15.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 4.0 14 16.0 15 33.0 16 72.0 17 217.0 18 467.0 19 898.0 20 1719.0 21 2646.0 22 4265.0 23 6402.0 24 10224.0 25 16713.0 26 26476.0 27 32842.0 28 32672.0 29 29701.0 30 28497.0 31 30423.0 32 35715.0 33 46463.0 34 84945.0 35 147140.0 36 68875.0 37 86743.0 38 146191.0 39 350499.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.080849134239116 22.78378650723007 26.36120114874964 18.774163209781168 2 37.70804292696035 23.36353559612381 27.248543069714326 11.67987840720152 3 26.883260836706246 22.981290831835814 39.3862418756193 10.749206455838637 4 23.88274020455805 25.693868296861094 37.756747224694756 12.6666442738861 5 19.750180541793323 29.9566699696018 37.855751305779016 12.437398182825858 6 18.273180726533766 40.02636749911828 33.92371899299666 7.776732781351292 7 79.08167206892497 3.336832205297012 15.893136052936532 1.688359672841476 8 79.06991585912702 2.897989688124549 15.714945501570293 2.3171489511781402 9 75.23579597937625 4.3615538350435825 17.566380598894916 2.836269586685253 10 39.51749156072083 26.560300958970828 24.09880254605915 9.823404934249199 11 32.61357338394102 22.462590060964345 32.61130611490856 12.312530440186084 12 30.61627731219455 21.117763633004717 35.447239809885296 12.818719244915439 13 19.40446400080614 31.923903733436337 35.22445963421393 13.44717263154359 14 13.651730682028115 32.54639504223838 39.17253077607779 14.629343499655711 15 13.569605159296644 23.837646742690158 49.39077641368423 13.201971684328973 16 14.537896982012999 21.80197504324606 46.333238164015924 17.32688981072502 17 16.44601380514922 21.32593251935576 36.744201669381795 25.483852006113228 18 16.861091983944377 24.477268528626368 41.56659892850545 17.095040558923802 19 20.969131551987637 25.476294442671684 36.407806123305846 17.146767882034833 20 21.545017886233477 24.439564684345765 37.558487143744856 16.456930285675895 21 17.888164855650537 26.176292763213137 39.85103202727441 16.084510353861916 22 18.83353206926087 23.39065885158625 35.40105469996473 22.37475437918815 23 15.665653393345986 26.39386661740255 36.92642292125019 21.014057068001275 24 14.626152528424836 24.92963068706649 45.38039735989117 15.063819424617503 25 15.583275951833128 25.431872764220813 41.57256100633156 17.4122902776145 26 13.998622843995095 29.25406848831937 36.8101204171775 19.937188250508036 27 14.749760677157683 31.19611037401542 36.05612750449255 17.998001444334346 28 12.997665552625834 28.100952252993633 41.110541961271686 17.790840233108845 29 13.972171371949681 25.33429622289774 39.542683438859314 21.150848966293267 30 16.065280553549535 29.978922795290796 37.19110558755857 16.764691063601095 31 19.107367784626238 30.290798246645284 34.136422417412625 16.465411551315857 32 20.638362192029287 25.69235678417278 35.972742387854154 17.69653863594377 33 21.202156424768653 27.62087902860118 32.559998656433166 18.616965890197 34 17.07648254202845 27.759014493727225 33.13756444922157 22.026938515022756 35 17.174143056278655 27.288178291318875 35.637690408612265 19.8999882437902 36 23.590178526443076 28.364879162957862 31.529566867641872 16.515375442957193 37 16.18964445862654 31.720101439295973 35.27324790487547 16.817006197202023 38 17.005945283240685 30.455889021379505 33.44994373813883 19.088221957240986 39 17.892867339569722 29.90998102211847 32.46351376316275 19.73363787514905 40 19.312261726819273 27.52682935021749 33.26226424600709 19.89864467695615 41 14.561241455754665 28.39250625598307 34.46223737467041 22.584014913591858 42 17.976672320843758 28.02084208051324 31.33323816401592 22.669247434627078 43 17.996909796281678 26.274876979661755 33.136808692877416 22.591404531179148 44 15.862317988680449 28.84881514199822 32.8471020942848 22.44176477503653 45 16.586500512234856 33.00136036141948 30.72317484842887 19.6889642779168 46 19.95868531985288 31.183514434946176 31.326940194481303 17.53086005071965 47 17.01677779084023 30.835362679072265 32.09453672136103 20.053322808726467 48 17.436306534773188 29.09930638362192 33.52955007305645 19.934837008548445 49 19.05152578808592 27.583259157247703 34.14591135817812 19.21930369648825 50 17.803604118032347 29.690223871823722 33.134961288480596 19.371210721663335 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1964.0 1 4591.0 2 7218.0 3 19390.5 4 31563.0 5 23048.0 6 14533.0 7 14515.5 8 14498.0 9 15007.5 10 15517.0 11 15643.5 12 15770.0 13 14629.0 14 13488.0 15 12640.0 16 11792.0 17 10982.5 18 10173.0 19 11772.5 20 13372.0 21 12105.5 22 10839.0 23 13822.0 24 16805.0 25 14792.0 26 12779.0 27 17161.0 28 21543.0 29 23957.0 30 26371.0 31 34920.0 32 43469.0 33 52932.5 34 62396.0 35 67790.5 36 73185.0 37 73426.0 38 73667.0 39 71842.0 40 70017.0 41 81387.5 42 92758.0 43 91847.5 44 90937.0 45 87872.5 46 84808.0 47 81059.5 48 77311.0 49 73754.5 50 70198.0 51 59772.0 52 49346.0 53 42023.0 54 34700.0 55 30058.0 56 25416.0 57 23834.5 58 22253.0 59 21203.0 60 20153.0 61 18640.0 62 17127.0 63 15246.5 64 13366.0 65 11699.0 66 10032.0 67 8568.0 68 7104.0 69 6248.0 70 5392.0 71 4468.5 72 3545.0 73 2980.0 74 2415.0 75 1969.0 76 1523.0 77 1135.5 78 748.0 79 529.0 80 310.0 81 269.0 82 228.0 83 142.0 84 56.0 85 96.0 86 136.0 87 79.5 88 23.0 89 15.5 90 8.0 91 6.0 92 4.0 93 3.0 94 2.0 95 1.5 96 1.0 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 1190860.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.788099509111944 #Duplication Level Percentage of deduplicated Percentage of total 1 78.09418168647359 20.13900528413449 2 8.862056261537688 4.570711774557649 3 3.437594285294701 2.6594707050340296 4 1.8262974087223711 1.8838695723746322 5 1.1242857597778988 1.449659652491499 6 0.7391497864710512 1.143676094739259 7 0.5616523889097383 1.0138763386314344 8 0.43026718247000834 0.8876618333633441 9 0.3392862179716686 0.7874592076011273 >10 3.5034505710011676 20.49111130004235 >50 0.6083158543400207 10.663308900004855 >100 0.4160433631561307 21.857479345980753 >500 0.04954459608562549 8.587320171507628 >1k 0.007546527880591962 2.41198700163561 >5k 0.0 0.0 >10k+ 3.281099078518244E-4 1.4534028179013148 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17177 1.4424029692827034 No Hit GATATGGGGTAGGGAAGATTTTTATGGCTTTCTGAATTGGAATTTCATAG 2156 0.1810456308886015 No Hit GATGCTGGGAACAAAAGCAATTGTAAATATATTATTAAGATGATTTATTA 1774 0.14896797272559326 No Hit TCCTTAGGGAGAACTTCAAAGAACATCTAATACCAATACTCCTCTAACTA 1496 0.12562349898392758 No Hit GCTTAGGGGGAACTACATTTTGATTATTCTCTCATCTATTAGAAAACATG 1438 0.12075306921048655 No Hit TGTGAAGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTA 1381 0.11596661236417379 No Hit GGTAGAGGGATAGGAAGAAAGATAAATAGATAATAGGTAAATAGACGATA 1376 0.11554674772853231 No Hit AGATAAGGGGGCTTTACCTACTTTTTAAAATTAAGTCTTTGATTTTATGA 1276 0.10714945501570294 No Hit TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1228 0.10311875451354482 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.08615622323362948 0.0 2 0.0 0.0 0.0 0.34470886586164623 0.0 3 0.0 0.0 0.0 0.5023260500814537 0.0 4 0.0 0.0 0.0 0.7542448314663353 0.0 5 0.0 0.0 0.0 1.3518801538384024 0.0 6 0.0 0.0 0.0 1.839007104109635 0.0 7 0.0 0.0 0.0 2.1257746502527586 0.0 8 0.0 0.0 0.0 2.830895319349042 0.0 9 0.0 0.0 0.0 3.082730127806795 0.0 10 0.0 0.0 0.0 3.5447491728666676 0.0 11 0.0 0.0 0.0 4.140956955477554 0.0 12 0.0 0.0 0.0 4.604991350788506 0.0 13 0.0 0.0 0.0 4.794098382681423 0.0 14 0.0 0.0 0.0 4.860353022185647 0.0 15 0.0 0.0 0.0 4.979846497489209 0.0 16 0.0 0.0 0.0 5.266194178996692 0.0 17 0.0 0.0 0.0 5.590497623566162 0.0 18 0.0 0.0 0.0 6.063181230371328 0.0 19 0.0 0.0 0.0 6.278907680163915 0.0 20 0.0 0.0 0.0 6.529566867641872 0.0 21 0.0 0.0 0.0 6.8798179466939855 0.0 22 0.0 0.0 0.0 7.228809431839175 0.0 23 0.0 0.0 0.0 7.637673614026838 0.0 24 0.0 0.0 0.0 7.9500529029440905 0.0 25 0.0 0.0 0.0 8.196345498211377 0.0 26 0.0 0.0 0.0 8.479754127269368 0.0 27 0.0 0.0 0.0 8.77886569370035 0.0 28 0.0 0.0 0.0 9.060342945434392 0.0 29 0.0 0.0 0.0 9.435785902624994 0.0 30 0.0 0.0 0.0 9.825084392791764 0.0 31 0.0 0.0 0.0 10.217657827116538 0.0 32 0.0 0.0 0.0 10.553045698066944 0.0 33 0.0 0.0 0.0 10.896410997094536 0.0 34 0.0 0.0 0.0 11.243051240280133 0.0 35 0.0 0.0 0.0 11.666106847152479 0.0 36 0.0 0.0 0.0 11.98201299900912 0.0 37 0.0 0.0 0.0 12.308667685538182 0.0 38 0.0 0.0 0.0 12.663957140217994 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGTGC 40 8.312782E-9 44.0 24 CTAGACG 25 4.4429376E-5 44.0 1 TCGTTGA 75 0.0 44.0 24 ACTGCCG 20 7.8565965E-4 44.0 21 CGTATTA 30 2.527795E-6 44.0 37 GTATTCG 35 1.4461511E-7 44.0 16 CGTATAG 20 7.8565965E-4 44.0 17 ACGTTGT 30 2.527795E-6 44.0 26 TAGACGA 170 0.0 44.0 42 GTTTACG 20 7.8565965E-4 44.0 1 CGTTGAT 75 0.0 44.0 25 ACTCTCG 20 7.8565965E-4 44.0 43 CGACAGG 45 4.802132E-10 44.0 2 TAACGCG 20 7.8565965E-4 44.0 1 GTTACGA 20 7.8565965E-4 44.0 23 CCGTATT 30 2.527795E-6 44.0 36 ATATCGG 20 7.8565965E-4 44.0 2 GTTAACG 25 4.4429376E-5 44.0 1 CGTAGGT 105 0.0 44.0 35 GTATGCG 45 4.802132E-10 44.0 1 >>END_MODULE