FastQCFastQC Report
Thu 26 May 2016
SRR1546243_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546243_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1820571
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT268181.4730543329537822No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG650.044.0000041
CGTATAA351.446715E-744.028
CAACCCG207.8576955E-444.023
CTAAGCG302.5285353E-644.01
CCGCTAT207.8576955E-444.025
CCCGTAT207.8576955E-444.029
CGAATCG207.8576955E-444.019
TTACGAG900.044.01
CGACTAT207.8576955E-444.023
GTCGGTT207.8576955E-444.039
ACGCGTA351.446715E-744.025
CATACGA1350.042.3703718
ACATACG1300.042.30769317
CGTTTTT164950.042.186121
ATGCGAT900.041.5555573
ACGATAC800.041.2534
ATGCGAG603.6379788E-1240.3333321
CCCTAGC11450.039.96506521
TGGGCGA3150.039.809526
GCCCTAG11100.039.6396420
Produced by FastQC (version 0.11.5)