##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546243_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1820571
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.87569449365062	31.0	31.0	34.0	30.0	34.0
2	32.23559971020081	33.0	31.0	34.0	30.0	34.0
3	32.15169581411546	33.0	31.0	34.0	30.0	34.0
4	35.90148530323728	37.0	35.0	37.0	35.0	37.0
5	35.906493072777714	37.0	35.0	37.0	35.0	37.0
6	35.964184862880934	37.0	35.0	37.0	35.0	37.0
7	36.19466420150601	37.0	35.0	37.0	35.0	37.0
8	36.080966356159685	37.0	35.0	37.0	35.0	37.0
9	37.80079216905026	39.0	38.0	39.0	35.0	39.0
10	37.405897929825315	39.0	37.0	39.0	34.0	39.0
11	37.30909038977332	39.0	37.0	39.0	34.0	39.0
12	37.06314886922839	39.0	37.0	39.0	33.0	39.0
13	36.9825730498838	39.0	37.0	39.0	33.0	39.0
14	38.138430195801206	40.0	37.0	41.0	33.0	41.0
15	38.244339275974404	40.0	37.0	41.0	33.0	41.0
16	38.291633229354964	40.0	37.0	41.0	34.0	41.0
17	38.207962776513526	40.0	37.0	41.0	33.0	41.0
18	38.01591423789569	39.0	36.0	41.0	33.0	41.0
19	37.857605113999945	39.0	36.0	41.0	34.0	41.0
20	37.62365928052243	39.0	35.0	41.0	33.0	41.0
21	37.52985464450439	39.0	35.0	41.0	33.0	41.0
22	37.44627921679517	39.0	35.0	41.0	33.0	41.0
23	37.421441404921865	39.0	35.0	41.0	33.0	41.0
24	37.358341970733356	39.0	35.0	41.0	33.0	41.0
25	37.3500621508307	39.0	35.0	41.0	33.0	41.0
26	37.29816195028922	39.0	35.0	41.0	33.0	41.0
27	37.23820988030678	39.0	35.0	41.0	33.0	41.0
28	37.14341214926526	39.0	35.0	41.0	33.0	41.0
29	37.04362916909036	39.0	35.0	41.0	32.0	41.0
30	36.85720633801154	39.0	35.0	41.0	32.0	41.0
31	36.60827839177928	39.0	35.0	41.0	31.0	41.0
32	36.328329958018664	39.0	35.0	41.0	30.0	41.0
33	35.99246664919962	39.0	35.0	41.0	29.0	41.0
34	35.563653930552555	39.0	35.0	41.0	25.0	41.0
35	35.36496791391272	39.0	35.0	41.0	23.0	41.0
36	35.23387827225634	39.0	35.0	41.0	23.0	41.0
37	35.208995968847134	39.0	35.0	41.0	23.0	41.0
38	35.11885996206684	39.0	35.0	41.0	23.0	41.0
39	35.024440134441335	39.0	35.0	41.0	22.0	41.0
40	34.88473671172396	38.0	35.0	41.0	21.0	41.0
41	34.77646134097489	38.0	34.0	41.0	21.0	41.0
42	34.74152010550536	38.0	34.0	41.0	21.0	41.0
43	34.62648257057813	38.0	34.0	41.0	20.0	41.0
44	34.46948567235224	38.0	34.0	40.0	18.0	41.0
45	34.40336246155739	38.0	34.0	40.0	20.0	41.0
46	34.32275972757997	38.0	34.0	40.0	20.0	41.0
47	34.29010898229182	38.0	34.0	40.0	20.0	41.0
48	34.2064154597651	38.0	34.0	40.0	20.0	41.0
49	34.13334607658806	38.0	34.0	40.0	19.0	41.0
50	34.021940918535996	38.0	34.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	3.0
11	8.0
12	4.0
13	12.0
14	21.0
15	46.0
16	92.0
17	240.0
18	547.0
19	1047.0
20	2079.0
21	3487.0
22	5575.0
23	8399.0
24	13117.0
25	22359.0
26	35926.0
27	44382.0
28	44324.0
29	40716.0
30	40008.0
31	43402.0
32	50609.0
33	64464.0
34	107299.0
35	167419.0
36	114076.0
37	148904.0
38	257089.0
39	604913.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.81811036207871	22.675358445235037	26.153113501203745	18.353417691482505
2	38.01521610527686	22.919128119694314	28.11914503746352	10.946510737565303
3	28.896593431401467	23.47911726595667	36.23863062742404	11.38565867521783
4	24.980294643823285	25.76559771632087	36.23088580450858	13.023221835347263
5	21.48672037509111	29.51552013077216	35.795692670046925	13.202066824089806
6	18.963116516741177	38.737736677119436	33.5902307572734	8.708916048865987
7	79.23810716527947	3.8128147707504954	15.231979417446503	1.7170986465235356
8	78.96791720839231	3.4325494583842104	15.089112152176432	2.5104211810470454
9	74.13833352283432	5.328712804938671	17.09172561795173	3.4412280542752796
10	34.62858630616438	28.71236551609358	24.8387456462835	11.820302531458537
11	27.505930831590746	26.093352030763974	32.595707610414536	13.805009527230744
12	25.856283550600335	23.140047820161914	36.59253058518454	14.411138044053212
13	20.580850733094177	27.678129553859748	37.271383538461286	14.469636174584789
14	15.848763931755478	30.004817169997764	38.50517227836761	15.641246619879148
15	15.21352366922246	25.543579459411358	44.5700826828506	14.67281418851558
16	18.07652654029972	23.21469473038953	41.69049160950053	17.018287119810214
17	17.857089891028693	24.06717452930976	36.520794849527974	21.554940730133566
18	18.691168869546974	25.827061949245593	39.65464681135754	15.8271223698499
19	20.730419192659884	26.204965365261778	36.138112712989496	16.926502729088842
20	21.0200535985688	26.143940554913815	36.83454256933676	16.001463277180623
21	19.898976749602184	25.60158323954408	38.634801938512695	15.86463807234104
22	18.316725906322798	24.339946093835398	36.844704216424404	20.4986237834174
23	16.659773224993696	26.500751687245376	36.83355386853905	20.005921219221882
24	16.300270629379465	24.862254754140324	41.96853624494732	16.868938371532888
25	17.40146360674755	27.120886798702166	37.56557695360412	17.912072640946164
26	16.06677245765202	28.359014836554024	36.22984217588877	19.344370529905177
27	15.940768033765233	28.40158389867794	37.39771752928065	18.25993053827618
28	15.14793984964058	28.206425346773074	38.80804428940151	17.837590514184836
29	16.51091882711523	26.32750933635656	37.68872513074195	19.47284670578626
30	17.557678332786804	28.502486307867148	37.11895883214662	16.82087652719943
31	19.489050413304398	28.9164223751779	34.385585621214446	17.20894159030326
32	19.61785615611805	27.027784140250503	35.68226671741998	17.672092986211467
33	19.811696440292632	28.486941734214156	34.00471610280511	17.6966457226881
34	17.059977336780605	27.935136833444012	35.827990229439	19.176895600336376
35	18.465580304201264	28.4923246607795	34.25754886791012	18.78454616710911
36	21.017856485684984	29.37534432878476	32.57307734771124	17.033721837819012
37	17.84610432660962	30.259132986299353	33.851632262625294	18.04313042446573
38	18.36204135955148	29.513817368287203	33.44274955494732	18.681391717213994
39	18.40279780354625	28.429926654879157	33.393424370705674	19.77385117086892
40	19.497509297907083	26.804008193033944	34.65753326840865	19.040949240650324
41	16.874760720675	28.107500339179303	33.30542999970888	21.71230894043682
42	17.852421026150587	28.671279505166236	32.59010497256081	20.88619449612237
43	18.211868693942723	27.95990928120903	32.8040488396223	21.02417318522595
44	18.009514597343358	28.78003659291508	32.488378646040175	20.72207016370139
45	18.047359866767074	30.795997519459554	31.5377977568576	19.61884485691577
46	19.571991424668415	30.275776116394255	32.209839660194525	17.942392798742812
47	18.119150530245733	29.663221044386624	33.323611108822455	18.894017316545195
48	18.091796474842234	29.60466798603295	33.602589517244866	18.70094602187995
49	18.904728241853793	27.80270585437206	34.39486842314856	18.897697480625585
50	18.059718626738533	28.198900235146006	33.88821419214082	19.853166945974642
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2015.0
1	5653.0
2	9291.0
3	29521.5
4	49752.0
5	35174.5
6	20597.0
7	20397.5
8	20198.0
9	20435.5
10	20673.0
11	19947.0
12	19221.0
13	18553.5
14	17886.0
15	16592.0
16	15298.0
17	15297.5
18	15297.0
19	14486.0
20	13675.0
21	14898.0
22	16121.0
23	19372.5
24	22624.0
25	24876.5
26	27129.0
27	35423.0
28	43717.0
29	50606.5
30	57496.0
31	71590.0
32	85684.0
33	94909.0
34	104134.0
35	104849.5
36	105565.0
37	108002.5
38	110440.0
39	109558.5
40	108677.0
41	116903.5
42	125130.0
43	121659.0
44	118188.0
45	114630.0
46	111072.0
47	111391.0
48	111710.0
49	108563.5
50	105417.0
51	90935.5
52	76454.0
53	65609.0
54	54764.0
55	49840.0
56	44916.0
57	42570.5
58	40225.0
59	38870.5
60	37516.0
61	35460.0
62	33404.0
63	29088.5
64	24773.0
65	21327.0
66	17881.0
67	15016.5
68	12152.0
69	10359.0
70	8566.0
71	7093.5
72	5621.0
73	4406.0
74	3191.0
75	2625.5
76	2060.0
77	1571.0
78	1082.0
79	805.5
80	529.0
81	356.5
82	184.0
83	155.0
84	126.0
85	89.0
86	52.0
87	39.0
88	26.0
89	23.5
90	21.0
91	17.5
92	14.0
93	8.5
94	3.0
95	2.5
96	2.0
97	1.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1820571.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.56945633111724
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.35632434464095	20.287460252795622
2	8.739071451005428	4.643847545840042
3	3.5205731456562446	2.8061914336205294
4	2.0419009208984944	2.170087894011225
5	1.3062728865326378	1.735348020762569
6	0.9549161982955798	1.5222962538294544
7	0.7412601971766317	1.3786416307215907
8	0.5718783164345699	1.2155596764176924
9	0.4911965547801196	1.1745742871003062
>10	4.357986550407014	24.545910378993526
>50	0.506398936880287	9.292156742227457
>100	0.36968485043245236	20.019649014192215
>500	0.040033549985545515	6.958670942024179
>1k	0.0022935888012552115	0.7639031726991808
>5k	0.0	0.0
>10k+	2.0850807284138287E-4	1.4857027547644235
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26818	1.4730543329537822	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0856874024687859	0.0
2	0.0	0.0	0.0	0.3073211646236263	0.0
3	0.0	0.0	0.0	0.4468378327458803	0.0
4	0.0	0.0	0.0	0.6745685831533074	0.0
5	0.0	0.0	0.0	1.244005314816066	0.0
6	0.0	0.0	0.0	1.770818056532813	0.0
7	0.0	0.0	0.0	2.086268538826555	0.0
8	0.0	0.0	0.0	2.734581623018273	0.0
9	0.0	0.0	0.0	3.002684322665801	0.0
10	0.0	0.0	0.0	3.5156552532145136	0.0
11	0.0	0.0	0.0	4.0997577133767376	0.0
12	0.0	0.0	0.0	4.589274463890725	0.0
13	0.0	0.0	0.0	4.792287694355233	0.0
14	0.0	0.0	0.0	4.866495182006085	0.0
15	0.0	0.0	0.0	4.990302493009062	0.0
16	0.0	0.0	0.0	5.261591006338121	0.0
17	0.0	0.0	0.0	5.595332453389624	0.0
18	0.0	0.0	0.0	6.038874616809781	0.0
19	0.0	0.0	0.0	6.260783018075099	0.0
20	0.0	0.0	0.0	6.501257023208653	0.0
21	0.0	0.0	0.0	6.829285976762235	0.0
22	0.0	0.0	0.0	7.172639792680428	0.0
23	0.0	0.0	0.0	7.546972900260413	0.0
24	0.0	0.0	0.0	7.850339261693172	0.0
25	0.0	0.0	0.0	8.104105799773807	0.0
26	0.0	0.0	0.0	8.36847340751885	0.0
27	0.0	0.0	0.0	8.647067321186595	0.0
28	5.492782209537557E-5	0.0	0.0	8.931813150929022	0.0
29	5.492782209537557E-5	0.0	0.0	9.266378515311954	0.0
30	5.492782209537557E-5	0.0	0.0	9.637690592676693	0.0
31	5.492782209537557E-5	0.0	0.0	9.95962255797769	0.0
32	5.492782209537557E-5	0.0	0.0	10.269580258061893	0.0
33	5.492782209537557E-5	0.0	0.0	10.600135891431863	0.0
34	5.492782209537557E-5	0.0	0.0	10.928549339740114	0.0
35	5.492782209537557E-5	0.0	0.0	11.310792053701833	0.0
36	5.492782209537557E-5	0.0	0.0	11.60789664341572	0.0
37	5.492782209537557E-5	0.0	0.0	11.930872237336528	0.0
38	5.492782209537557E-5	0.0	0.0	12.286474957581989	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	65	0.0	44.000004	1
CGTATAA	35	1.446715E-7	44.0	28
CAACCCG	20	7.8576955E-4	44.0	23
CTAAGCG	30	2.5285353E-6	44.0	1
CCGCTAT	20	7.8576955E-4	44.0	25
CCCGTAT	20	7.8576955E-4	44.0	29
CGAATCG	20	7.8576955E-4	44.0	19
TTACGAG	90	0.0	44.0	1
CGACTAT	20	7.8576955E-4	44.0	23
GTCGGTT	20	7.8576955E-4	44.0	39
ACGCGTA	35	1.446715E-7	44.0	25
CATACGA	135	0.0	42.37037	18
ACATACG	130	0.0	42.307693	17
CGTTTTT	16495	0.0	42.18612	1
ATGCGAT	90	0.0	41.555557	3
ACGATAC	80	0.0	41.25	34
ATGCGAG	60	3.6379788E-12	40.333332	1
CCCTAGC	1145	0.0	39.965065	21
TGGGCGA	315	0.0	39.80952	6
GCCCTAG	1110	0.0	39.63964	20
>>END_MODULE