FastQCFastQC Report
Thu 26 May 2016
SRR1546242_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546242_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1863782
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT274301.4717386475456893No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18740.10054824008387246No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTCGA408.3164196E-944.014
CATTACG302.528568E-644.020
TATACGC207.857744E-444.039
TAGTCGG254.443909E-544.02
ACGACTT254.443909E-544.039
GTCGGTA207.857744E-444.026
CGTTTTT163200.042.4497571
TACGCGG1600.041.252
GTCAACG1500.041.0666661
TTAATCG700.040.8571420
CATACGA2250.040.0888918
ATTACGC557.8216544E-1140.038
ACGATAC1100.040.034
TATTACG557.8216544E-1140.037
AGTAGCG1100.040.01
GTACGCG1550.039.7419361
CATTCGA950.039.3684225
TCACGAC1400.039.2857125
TATCGCT452.3523171E-839.1111135
AACGGGA6500.038.9230774
Produced by FastQC (version 0.11.5)