##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546242_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1863782
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.817857453285846	31.0	31.0	34.0	30.0	34.0
2	32.20726404697545	33.0	31.0	34.0	30.0	34.0
3	32.24415999296055	33.0	31.0	34.0	30.0	34.0
4	35.94142179718443	37.0	35.0	37.0	35.0	37.0
5	35.87231017361473	37.0	35.0	37.0	35.0	37.0
6	35.95615688959331	37.0	35.0	37.0	35.0	37.0
7	36.176972414155735	37.0	35.0	37.0	35.0	37.0
8	36.06741292704834	37.0	35.0	37.0	35.0	37.0
9	37.77831366543941	39.0	38.0	39.0	35.0	39.0
10	37.40641823990145	39.0	37.0	39.0	34.0	39.0
11	37.306524046267214	39.0	37.0	39.0	34.0	39.0
12	37.048340954038615	39.0	37.0	39.0	33.0	39.0
13	36.948529388093675	39.0	37.0	39.0	33.0	39.0
14	38.089167617242786	40.0	37.0	41.0	33.0	41.0
15	38.219951689628935	40.0	37.0	41.0	33.0	41.0
16	38.259103264222965	40.0	37.0	41.0	33.0	41.0
17	38.17800472372842	40.0	37.0	41.0	33.0	41.0
18	38.0048299640194	39.0	36.0	41.0	33.0	41.0
19	37.8517380251553	39.0	36.0	41.0	34.0	41.0
20	37.61627969365516	39.0	35.0	41.0	33.0	41.0
21	37.52180941762502	39.0	35.0	41.0	33.0	41.0
22	37.446676703605895	39.0	35.0	41.0	33.0	41.0
23	37.40873879026624	39.0	35.0	41.0	33.0	41.0
24	37.35006186345828	39.0	35.0	41.0	33.0	41.0
25	37.328697240342485	39.0	35.0	41.0	33.0	41.0
26	37.29957741838906	39.0	35.0	41.0	33.0	41.0
27	37.23265864784615	39.0	35.0	41.0	33.0	41.0
28	37.13476576123173	39.0	35.0	41.0	33.0	41.0
29	37.041363743184554	39.0	35.0	41.0	32.0	41.0
30	36.853643827443335	39.0	35.0	41.0	32.0	41.0
31	36.612949368542026	39.0	35.0	41.0	31.0	41.0
32	36.34166603175693	39.0	35.0	41.0	30.0	41.0
33	35.99071833508425	39.0	35.0	41.0	29.0	41.0
34	35.63691193497952	39.0	35.0	41.0	25.0	41.0
35	35.40910578597712	39.0	35.0	41.0	24.0	41.0
36	35.27170237720935	39.0	35.0	41.0	23.0	41.0
37	35.18296399471612	39.0	35.0	41.0	23.0	41.0
38	35.094437546880485	39.0	35.0	41.0	23.0	41.0
39	35.006524368193276	39.0	35.0	41.0	22.0	41.0
40	34.88750240103188	39.0	35.0	41.0	21.0	41.0
41	34.804332266327286	38.0	34.0	41.0	21.0	41.0
42	34.74736744962662	38.0	34.0	41.0	21.0	41.0
43	34.66195402681215	38.0	34.0	41.0	20.0	41.0
44	34.51533924031888	38.0	34.0	40.0	19.0	41.0
45	34.436175475457965	38.0	34.0	40.0	20.0	41.0
46	34.36616299545762	38.0	34.0	40.0	20.0	41.0
47	34.30771088034974	38.0	34.0	40.0	20.0	41.0
48	34.24349306946843	38.0	34.0	40.0	20.0	41.0
49	34.164100737103375	38.0	34.0	40.0	20.0	41.0
50	34.0236765887856	37.0	34.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	2.0
11	5.0
12	5.0
13	12.0
14	12.0
15	53.0
16	87.0
17	255.0
18	564.0
19	1132.0
20	2056.0
21	3537.0
22	5496.0
23	8281.0
24	13344.0
25	22655.0
26	35782.0
27	45298.0
28	45530.0
29	41926.0
30	41238.0
31	44241.0
32	52537.0
33	66481.0
34	112531.0
35	169609.0
36	117834.0
37	153907.0
38	263728.0
39	615638.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.029292052396684	22.692299850519	26.09516563632442	18.183242460759896
2	38.06952744473334	22.88089486860588	28.117397850177756	10.932179836483021
3	28.958805267998084	23.46384931284882	36.14022455415923	11.437120864993867
4	25.143391233524092	25.696782134391256	36.14736058187062	13.012466050214028
5	21.528805407499373	29.57126960127311	35.64161473820436	13.258310253023154
6	19.068324514347708	38.79198318258251	33.43668948407056	8.703002818999218
7	79.31920149459539	3.8131605520388114	15.16550755399505	1.702130399370742
8	78.99110518290229	3.4239519428774394	15.050901875863165	2.5340409983571037
9	74.15894133541369	5.328734798383072	17.044858250589392	3.4674656156138433
10	34.65990121162239	28.680178261191493	24.7543972417375	11.90552328544862
11	27.57226971823958	26.062436486670652	32.55176839351384	13.813525401575935
12	25.850394520389187	23.190641394755396	36.53705208012525	14.421912004730167
13	20.800125765781623	27.522424832947202	37.19716147060117	14.480287930670002
14	15.925950567180067	29.96917021411302	38.44280071381739	15.662078504889521
15	15.271957771885338	25.55867585372109	44.48626502455759	14.683101349835978
16	18.2480569079431	23.218380690445557	41.487470101116976	17.04609230049437
17	17.919531361500432	24.075133250562565	36.41182284194181	21.593512545995186
18	18.723005158328604	25.829415672004558	39.53911991853125	15.908459251135595
19	20.779898078208717	26.24749031807368	36.09096986664749	16.881641737070108
20	21.05101347689805	26.18428550120132	36.734607373609144	16.030093648291484
21	19.90114723717688	25.600955476552517	38.590618430696296	15.907278855574312
22	18.29800910192286	24.358213567895817	36.83349232903848	20.510285001142837
23	16.654791171928906	26.51511818442286	36.85001786689645	19.980072776751786
24	16.407069067090465	24.86465691803011	41.75917569758695	16.96909831729247
25	17.48487752322965	27.101828432724428	37.50604952725158	17.907244516794346
26	16.12656415825456	28.345267847849158	36.13115697007483	19.397011023821456
27	15.919458391593007	28.261513417341728	37.4976794496352	18.32134874143006
28	15.188096032690519	28.166276957283632	38.669061081177944	17.9765659288479
29	16.50842212232976	26.394932454546723	37.633961482619746	19.462683940503773
30	17.585050182907658	28.458746784763456	37.082448483781896	16.873754548546987
31	19.491281705693048	28.95821507021744	34.333575493271205	17.216927730818306
32	19.573855740639196	27.03942843100749	35.639629527487656	17.747086300865657
33	19.848887906418238	28.457995623951724	33.98535880269259	17.707757666937443
34	16.985409237775663	27.99662192252098	35.7404996936337	19.27746914606966
35	18.500768866745148	28.47527232262142	34.1877966414527	18.83616216918073
36	20.962161883739622	29.41851568477429	32.5176978852677	17.10162454621839
37	17.853482864412253	30.27569747964086	33.72556447052284	18.14525518542405
38	18.40038158969236	29.447649993400514	33.439694127317466	18.71227428958966
39	18.360623721014584	28.334483324766524	33.416247179122884	19.888645775096016
40	19.521811027255335	26.738749488942375	34.705668366793965	19.033771117008317
41	16.852561082787577	28.06959182994578	33.29423720156113	21.78360988570552
42	17.82901648368747	28.676368802789163	32.58481946922977	20.90979524429359
43	18.220317612252934	27.94012389861046	32.84434552968104	20.995212959455557
44	18.060320359355334	28.70571772879017	32.48759779845497	20.74636411339953
45	18.04100479562524	30.743402393627584	31.506045234904086	19.709547575843096
46	19.55577422681408	30.171232472467274	32.236602778651154	18.036390522067496
47	18.06885140000279	29.672837273887183	33.25227950479187	19.00603182131816
48	18.057959568232764	29.599867366462384	33.55853849860123	18.783634566703615
49	18.92871591205409	27.759791649452563	34.361636715023536	18.949855723469806
50	18.072124314968168	28.192674894381426	33.795583389044424	19.939617401605982
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2066.0
1	5809.5
2	9553.0
3	30037.0
4	50521.0
5	35516.0
6	20511.0
7	20627.0
8	20743.0
9	20866.5
10	20990.0
11	20339.5
12	19689.0
13	18968.5
14	18248.0
15	16953.0
16	15658.0
17	15740.0
18	15822.0
19	14830.0
20	13838.0
21	15114.5
22	16391.0
23	19737.5
24	23084.0
25	25285.0
26	27486.0
27	35938.5
28	44391.0
29	51632.5
30	58874.0
31	72758.0
32	86642.0
33	95938.5
34	105235.0
35	106399.0
36	107563.0
37	110128.0
38	112693.0
39	111436.0
40	110179.0
41	118841.0
42	127503.0
43	124235.5
44	120968.0
45	117705.0
46	114442.0
47	115295.5
48	116149.0
49	112941.0
50	109733.0
51	94758.5
52	79784.0
53	68592.5
54	57401.0
55	51810.5
56	46220.0
57	43880.5
58	41541.0
59	39944.5
60	38348.0
61	36187.0
62	34026.0
63	29449.5
64	24873.0
65	21473.0
66	18073.0
67	15265.0
68	12457.0
69	10634.0
70	8811.0
71	7323.0
72	5835.0
73	4614.5
74	3394.0
75	2731.0
76	2068.0
77	1557.5
78	1047.0
79	795.0
80	543.0
81	366.0
82	189.0
83	142.0
84	95.0
85	75.5
86	56.0
87	37.0
88	18.0
89	16.5
90	15.0
91	14.5
92	14.0
93	7.0
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1863782.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	26.480742105855263
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.16709807864035	20.169612811718597
2	8.812988539833649	4.667489534103857
3	3.643992020098008	2.894868387600297
4	2.0312871745279737	2.151599672464268
5	1.3261325691857004	1.7558487281390902
6	0.9290765204836066	1.476158144131905
7	0.7421262059869942	1.375643686951689
8	0.5727509281844759	1.2133495696113872
9	0.48771751993266566	1.162360967925982
>10	4.3587600076412585	24.43088289115606
>50	0.5145355930875151	9.385597384333092
>100	0.371320686562079	20.08472888521848
>500	0.03975507879670463	6.963955752478311
>1k	0.0022541539523904686	0.7794104079484928
>5k	0.0	0.0
>10k+	2.049230865809517E-4	1.4884931762185587
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27430	1.4717386475456893	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1874	0.10054824008387246	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08756388890975447	0.0
2	0.0	0.0	0.0	0.3032543505624585	0.0
3	0.0	0.0	0.0	0.44667241125839824	0.0
4	0.0	0.0	0.0	0.6755618414599991	0.0
5	0.0	0.0	0.0	1.2495023559622316	0.0
6	0.0	0.0	0.0	1.7851336690664465	0.0
7	0.0	0.0	0.0	2.1052354835490417	0.0
8	0.0	0.0	0.0	2.771193197487689	0.0
9	0.0	0.0	0.0	3.0459570915482606	0.0
10	0.0	0.0	0.0	3.5677455839792422	0.0
11	0.0	0.0	0.0	4.188043451433698	0.0
12	0.0	0.0	0.0	4.687511736887683	0.0
13	0.0	0.0	0.0	4.898802542357422	0.0
14	0.0	0.0	0.0	4.976333068996267	0.0
15	0.0	0.0	0.0	5.10735697629873	0.0
16	0.0	0.0	0.0	5.39419309769061	0.0
17	5.365434369470249E-5	0.0	0.0	5.739405145022325	0.0
18	5.365434369470249E-5	0.0	0.0	6.20029595735982	0.0
19	5.365434369470249E-5	0.0	0.0	6.43122425262182	0.0
20	5.365434369470249E-5	0.0	0.0	6.688926065387475	0.0
21	5.365434369470249E-5	0.0	0.0	7.030865197753815	0.0
22	5.365434369470249E-5	0.0	0.0	7.391905276475468	0.0
23	5.365434369470249E-5	0.0	0.0	7.7845477636333005	0.0
24	5.365434369470249E-5	0.0	0.0	8.103522836898307	0.0
25	5.365434369470249E-5	0.0	0.0	8.372384753152462	0.0
26	5.365434369470249E-5	0.0	0.0	8.64586094296436	0.0
27	5.365434369470249E-5	0.0	0.0	8.935111509822502	0.0
28	5.365434369470249E-5	0.0	0.0	9.230371363174449	0.0
29	5.365434369470249E-5	0.0	0.0	9.578480745065677	0.0
30	5.365434369470249E-5	0.0	0.0	9.963772587137337	0.0
31	5.365434369470249E-5	0.0	0.0	10.296375863700797	0.0
32	5.365434369470249E-5	0.0	0.0	10.621413877803306	0.0
33	5.365434369470249E-5	0.0	0.0	10.97140116172385	0.0
34	5.365434369470249E-5	0.0	0.0	11.314627998338862	0.0
35	5.365434369470249E-5	0.0	0.0	11.724064295073136	0.0
36	5.365434369470249E-5	0.0	0.0	12.030055017164024	0.0
37	5.365434369470249E-5	0.0	0.0	12.356756315921068	0.0
38	5.365434369470249E-5	0.0	0.0	12.73169286965965	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTCGA	40	8.3164196E-9	44.0	14
CATTACG	30	2.528568E-6	44.0	20
TATACGC	20	7.857744E-4	44.0	39
TAGTCGG	25	4.443909E-5	44.0	2
ACGACTT	25	4.443909E-5	44.0	39
GTCGGTA	20	7.857744E-4	44.0	26
CGTTTTT	16320	0.0	42.449757	1
TACGCGG	160	0.0	41.25	2
GTCAACG	150	0.0	41.066666	1
TTAATCG	70	0.0	40.85714	20
CATACGA	225	0.0	40.08889	18
ATTACGC	55	7.8216544E-11	40.0	38
ACGATAC	110	0.0	40.0	34
TATTACG	55	7.8216544E-11	40.0	37
AGTAGCG	110	0.0	40.0	1
GTACGCG	155	0.0	39.741936	1
CATTCGA	95	0.0	39.36842	25
TCACGAC	140	0.0	39.28571	25
TATCGCT	45	2.3523171E-8	39.11111	35
AACGGGA	650	0.0	38.923077	4
>>END_MODULE