FastQCFastQC Report
Thu 26 May 2016
SRR1546241_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546241_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1432538
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198921.3885844563983643No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTACAGGTCGTATGCC15050.1050582951377206No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTA408.314601E-944.011
TCGTTAG254.4433902E-544.01
GATCGAC207.857132E-444.010
CGCATGG1000.044.02
TAAGTCG351.446424E-744.038
ACGGGTA2950.044.05
GTTACCG207.857132E-444.038
GTAGCGT207.857132E-444.09
ACCGTAC502.7284841E-1144.029
TTCGACT302.5281552E-644.041
CGTCAAC207.857132E-444.026
GTAACGA302.5281552E-644.036
ACCTTAC207.857132E-444.010
CGGGTAT3800.043.421056
AGTACGG2200.043.0000042
CGTTTTT129750.042.3383451
TACGGGT1150.042.0869524
ACATACG6750.042.04444517
ACGGGAT4950.041.777785
TACGGGA4550.041.582424
Produced by FastQC (version 0.11.5)