##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546241_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1432538
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.856071531784846	31.0	31.0	34.0	30.0	34.0
2	32.21667418246497	33.0	31.0	34.0	30.0	34.0
3	32.16744477284372	33.0	31.0	34.0	30.0	34.0
4	35.90638991775436	37.0	35.0	37.0	35.0	37.0
5	35.90317604140344	37.0	35.0	37.0	35.0	37.0
6	35.9593169605274	37.0	35.0	37.0	35.0	37.0
7	36.167804972712766	37.0	35.0	37.0	35.0	37.0
8	36.073471000420234	37.0	35.0	37.0	35.0	37.0
9	37.83130011210872	39.0	38.0	39.0	35.0	39.0
10	37.40625100346378	39.0	37.0	39.0	34.0	39.0
11	37.27498188529729	39.0	37.0	39.0	34.0	39.0
12	36.964590119075375	39.0	35.0	39.0	33.0	39.0
13	36.85148386988687	39.0	35.0	39.0	33.0	39.0
14	38.011689044199876	40.0	36.0	41.0	33.0	41.0
15	38.143975238353185	40.0	36.0	41.0	33.0	41.0
16	38.212070465146475	40.0	36.0	41.0	33.0	41.0
17	38.12694532361445	40.0	36.0	41.0	33.0	41.0
18	37.94964531481887	39.0	36.0	41.0	33.0	41.0
19	37.762850269940486	39.0	36.0	41.0	34.0	41.0
20	37.514612526857924	39.0	35.0	41.0	33.0	41.0
21	37.43800164463351	39.0	35.0	41.0	33.0	41.0
22	37.36225566093186	39.0	35.0	41.0	33.0	41.0
23	37.30921204184462	39.0	35.0	41.0	33.0	41.0
24	37.26791191577466	39.0	35.0	41.0	33.0	41.0
25	37.24675436183892	39.0	35.0	41.0	33.0	41.0
26	37.19726248099527	39.0	35.0	41.0	33.0	41.0
27	37.127358576177386	39.0	35.0	41.0	33.0	41.0
28	37.04797289845016	39.0	35.0	41.0	33.0	41.0
29	36.95540083404419	39.0	35.0	41.0	32.0	41.0
30	36.77084377517385	39.0	35.0	41.0	32.0	41.0
31	36.49102362380614	39.0	35.0	41.0	31.0	41.0
32	36.2047352321544	39.0	35.0	41.0	30.0	41.0
33	35.84158046767346	39.0	35.0	41.0	28.0	41.0
34	35.42230921623022	39.0	35.0	41.0	24.0	41.0
35	35.19178060198054	39.0	35.0	41.0	23.0	41.0
36	35.01383558411714	38.0	35.0	41.0	22.0	41.0
37	34.986033180271654	39.0	35.0	41.0	22.0	41.0
38	34.90231951962182	38.0	35.0	41.0	21.0	41.0
39	34.81267791849152	38.0	35.0	41.0	21.0	41.0
40	34.67864936218097	38.0	34.0	41.0	20.0	41.0
41	34.564761283819344	38.0	34.0	41.0	18.0	41.0
42	34.516923111289195	38.0	34.0	41.0	18.0	41.0
43	34.40933713451231	38.0	34.0	41.0	18.0	41.0
44	34.309304884058925	38.0	34.0	40.0	18.0	41.0
45	34.25796034729969	38.0	34.0	40.0	18.0	41.0
46	34.192954741863744	38.0	34.0	40.0	18.0	41.0
47	34.183402464716465	38.0	34.0	40.0	18.0	41.0
48	34.123093418813326	38.0	34.0	40.0	18.0	41.0
49	34.03979231266465	37.0	34.0	40.0	18.0	41.0
50	33.89296060558254	37.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	4.0
11	3.0
12	3.0
13	10.0
14	17.0
15	27.0
16	90.0
17	199.0
18	506.0
19	977.0
20	1799.0
21	2927.0
22	4746.0
23	7416.0
24	11402.0
25	18889.0
26	29829.0
27	36548.0
28	35569.0
29	32125.0
30	31646.0
31	34122.0
32	39476.0
33	50620.0
34	87010.0
35	146600.0
36	85092.0
37	112267.0
38	191657.0
39	470960.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.83475761201448	22.492387636488527	27.1643055891013	18.508549162395692
2	36.77361438230609	23.764675003385598	28.030530429210256	11.431180185098057
3	26.803686883000662	23.2002920690411	39.35071879419604	10.645302253762203
4	23.465695150844166	26.594128742134586	37.95522352635672	11.984952580664526
5	20.309478701437587	30.118642577020644	36.756511869144134	12.815366852397633
6	17.95791804475693	40.13959839110725	33.54563718379547	8.356846380340347
7	80.26139620729083	3.2272791367489027	14.94145356004518	1.5698710959150823
8	80.65517284707282	2.8052309956175683	14.590188881551484	1.94940727575813
9	76.40851411969525	4.09762254125196	16.317821935613573	3.1760414034392106
10	35.08584065483778	30.596884689969826	24.16955082517881	10.147723830013584
11	28.065852354352906	23.324198031745055	33.99637566333319	14.613573950568851
12	28.437640048640944	21.489761528140964	37.07971446481699	12.992883958401102
13	19.91193252814236	29.256955138362823	35.44722722887631	15.383885104618516
14	14.595912987997526	31.674343019172962	36.30877505518178	17.420968937647725
15	13.193855939598112	25.944303048156485	44.79657782201938	16.065263190226016
16	16.004462010780866	23.123575081428903	41.3575765529431	19.514386354847133
17	16.415899613134172	22.923091743465097	36.63044191497887	24.030566728421864
18	17.25922802745896	26.112675545081526	40.97182762342081	15.656268804038707
19	19.33002824357888	28.00777361577843	34.18492214517171	18.477275995470976
20	21.15922928397013	24.243964208977353	37.48389222484849	17.112914282204034
21	18.0703757945688	26.75147186322457	38.04883360860236	17.129318733604272
22	18.753568840756753	24.119290378335513	35.251490710892135	21.875650070015595
23	15.888513952160432	26.73897655768992	35.73887743291975	21.633632057229896
24	14.326181923271845	25.332731138720227	45.13311339734094	15.207973540666982
25	15.445733376706238	30.77223780451199	36.98631380109987	16.795715017681903
26	16.516141282116077	27.745721230431585	35.749627584050124	19.988509903402214
27	14.327368628266754	30.227121374790755	35.50418906863204	19.941320928310454
28	13.092846402678324	28.777247095714042	38.70570972637375	19.424196775233884
29	14.665998388873454	25.119124239636225	37.68863373955874	22.526243631931578
30	16.185120394712044	29.458415762793027	37.24166479353428	17.114799048960656
31	18.400140170801752	32.79473214672141	32.27502516512651	16.530102517350326
32	19.18692558242783	27.319135687849116	33.061182321167045	20.432756408556003
33	18.44823662618374	31.221231129645428	31.735214004794287	18.595318239376546
34	16.646259994499275	27.319903555787	34.4699407624789	21.563895687234826
35	18.716152730328968	28.346193957856613	33.24721578066341	19.690437531151005
36	22.0135172679538	30.667807764959814	30.764559125133157	16.554115841953234
37	16.632717596322053	33.11067490007245	33.76601528196809	16.4905922216374
38	17.05602224862447	29.335626698907813	35.751861381687604	17.856489670780114
39	16.706293306006543	31.63211028258936	32.72541461378337	18.936181797620726
40	18.77862925800223	28.239948957723982	33.66821682915217	19.31320495512161
41	16.559351305166075	29.233779487873967	33.30222304748635	20.904646159473607
42	17.37357054402745	28.744717417618244	31.358539878174263	22.523172160180042
43	16.965623250482707	28.059779217026005	32.8911344760139	22.083463056477385
44	16.020936268357282	29.399429543928328	32.77092824064702	21.808705947067374
45	16.487520749885867	34.667213016338835	28.978568107791908	19.866698125983394
46	18.30450570944715	31.542688570914002	32.74174925900744	17.41105646063141
47	16.941958956760658	31.42813663581699	33.20149273527125	18.428411672151103
48	16.340299524340715	28.874696517649095	34.947973456899575	19.83703050111062
49	17.78675330078504	28.57439034776041	34.43357174469369	19.205284606760866
50	17.446867029007258	29.789646068725578	33.81655495351607	18.946931948751097
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1728.0
1	4434.0
2	7140.0
3	22570.5
4	38001.0
5	27403.5
6	16806.0
7	16773.5
8	16741.0
9	16988.0
10	17235.0
11	16757.5
12	16280.0
13	15068.5
14	13857.0
15	12975.5
16	12094.0
17	11504.5
18	10915.0
19	10593.5
20	10272.0
21	10369.5
22	10467.0
23	12322.0
24	14177.0
25	19345.5
26	24514.0
27	26919.0
28	29324.0
29	37444.0
30	45564.0
31	53632.5
32	61701.0
33	74119.5
34	86538.0
35	94431.5
36	102325.0
37	98724.5
38	95124.0
39	97564.5
40	100005.0
41	100485.5
42	100966.0
43	97889.5
44	94813.0
45	92301.5
46	89790.0
47	87107.0
48	84424.0
49	79929.5
50	75435.0
51	64195.0
52	52955.0
53	45510.5
54	38066.0
55	34239.0
56	30412.0
57	29608.0
58	28804.0
59	27277.0
60	25750.0
61	23599.5
62	21449.0
63	19858.0
64	18267.0
65	16430.0
66	14593.0
67	12273.0
68	9953.0
69	7991.5
70	6030.0
71	5136.0
72	4242.0
73	3518.5
74	2795.0
75	2115.0
76	1435.0
77	1077.5
78	720.0
79	579.5
80	439.0
81	325.0
82	211.0
83	147.5
84	84.0
85	75.5
86	67.0
87	43.5
88	20.0
89	14.0
90	8.0
91	4.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1432538.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.665015650781537
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.95027130949659	20.00594933140897
2	8.577537685707862	4.4028527789772145
3	3.212324730888038	2.4733309348090224
4	1.714837786581298	1.7604535452464234
5	1.12523860503771	1.4439633204578206
6	0.7747083472180677	1.1929741113685683
7	0.5972007060950897	1.0729015828011783
8	0.4957009646593606	1.0177738412871995
9	0.4046968282920973	0.9347895388744494
>10	4.133293519724404	23.062956878627457
>50	0.5124368121707256	9.052028167872612
>100	0.45738374230276546	24.825159677259453
>500	0.04190401950234998	6.675207727494474
>1k	0.0021910598432601297	0.6814104682862078
>5k	0.0	0.0
>10k+	2.738824804075162E-4	1.3982480952289298
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19892	1.3885844563983643	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTACAGGTCGTATGCC	1505	0.1050582951377206	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0725286170419214	0.0
2	0.0	0.0	0.0	0.2899748558153431	0.0
3	0.0	0.0	0.0	0.4193955064368275	0.0
4	0.0	0.0	0.0	0.6268594620177614	0.0
5	0.0	0.0	0.0	1.143076134804103	0.0
6	0.0	0.0	0.0	1.5277779716838227	0.0
7	0.0	0.0	0.0	1.7889228767404426	0.0
8	0.0	0.0	0.0	2.3365523288038434	0.0
9	0.0	0.0	0.0	2.556302171390916	0.0
10	0.0	0.0	0.0	3.075799734457306	0.0
11	0.0	0.0	0.0	3.5844773402171533	0.0
12	0.0	0.0	0.0	4.027886171256888	0.0
13	0.0	0.0	0.0	4.186276384989438	0.0
14	0.0	0.0	0.0	4.251405547357208	0.0
15	0.0	0.0	0.0	4.362886010702683	0.0
16	0.0	0.0	0.0	4.594712321767381	0.0
17	0.0	0.0	0.0	4.864582998845406	0.0
18	0.0	0.0	0.0	5.242932473693543	0.0
19	0.0	0.0	0.0	5.431409149355899	0.0
20	0.0	0.0	0.0	5.636918532004038	0.0
21	0.0	0.0	0.0	5.914537694637071	0.0
22	0.0	0.0	0.0	6.217566305396436	0.0
23	0.0	0.0	0.0	6.548796611328984	0.0
24	0.0	0.0	0.0	6.806730432281727	0.0
25	0.0	0.0	0.0	7.03778887540854	0.0
26	0.0	0.0	0.0	7.281133205541494	0.0
27	0.0	0.0	0.0	7.525035985083816	0.0
28	0.0	0.0	0.0	7.7622373717137	0.0
29	0.0	0.0	0.0	8.056889241332517	0.0
30	0.0	0.0	0.0	8.402918456613367	0.0
31	0.0	0.0	0.0	8.691636801257628	0.0
32	0.0	0.0	0.0	8.95794736335092	0.0
33	0.0	0.0	0.0	9.23717206803589	0.0
34	0.0	0.0	0.0	9.533359673530475	0.0
35	0.0	0.0	0.0	9.915827712772716	0.0
36	0.0	0.0	0.0	10.184441878679658	0.0
37	0.0	0.0	0.0	10.465272125416568	0.0
38	0.0	0.0	0.0	10.772698525274722	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTA	40	8.314601E-9	44.0	11
TCGTTAG	25	4.4433902E-5	44.0	1
GATCGAC	20	7.857132E-4	44.0	10
CGCATGG	100	0.0	44.0	2
TAAGTCG	35	1.446424E-7	44.0	38
ACGGGTA	295	0.0	44.0	5
GTTACCG	20	7.857132E-4	44.0	38
GTAGCGT	20	7.857132E-4	44.0	9
ACCGTAC	50	2.7284841E-11	44.0	29
TTCGACT	30	2.5281552E-6	44.0	41
CGTCAAC	20	7.857132E-4	44.0	26
GTAACGA	30	2.5281552E-6	44.0	36
ACCTTAC	20	7.857132E-4	44.0	10
CGGGTAT	380	0.0	43.42105	6
AGTACGG	220	0.0	43.000004	2
CGTTTTT	12975	0.0	42.338345	1
TACGGGT	115	0.0	42.086952	4
ACATACG	675	0.0	42.044445	17
ACGGGAT	495	0.0	41.77778	5
TACGGGA	455	0.0	41.58242	4
>>END_MODULE