FastQCFastQC Report
Thu 26 May 2016
SRR1546240_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546240_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1462946
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206311.4102366047687338No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTCGC650.044.0000049
TCGCCGA207.857188E-444.044
TAATACG302.5281934E-644.01
TCGCAAG408.314601E-944.01
TTGTACG408.314601E-944.01
ACGGGTA2250.044.05
AACGGCG302.5281934E-644.014
TTGCGTG1000.044.02
TATTACG1250.044.01
CGTACCA302.5281934E-644.043
TATAGCG750.044.01
CCAACGG207.857188E-444.02
AGTCGAA207.857188E-444.018
AAGTCGA207.857188E-444.017
CGGGTAT3150.043.9999966
CGTTTTT131050.042.4219781
CGACGGT1150.042.08695228
GATCGCT1050.041.9047610
ACGGGAT4750.041.221055
TACGGGA5250.041.066674
Produced by FastQC (version 0.11.5)