FastQCFastQC Report
Thu 26 May 2016
SRR1546239_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546239_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2039759
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT285171.3980573195166683No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC48760.2390478482997256No Hit
GAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCT40370.1979155380611141No Hit
GGGAAAGGGCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG31710.15545954203413245No Hit
TATTTTGGGTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAAT26790.13133904544605515No Hit
TTGAATGGGAGTGAAACAGACTATAAAAGTTTTCTTCCAATTTTTAGCTA26720.1309958676490703No Hit
GAAAAAGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATT24180.11854341615847755No Hit
GATGGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT24060.11795511136364639No Hit
TACGAGAGGGACTGAATAAAATTTTCCAATCAAAATTCCTATAGTCTTAT24050.11790608596407713No Hit
TGATTAGGGATTCTTTTCCTGATCACTATGCATTTTGAACATTTTTTTAA23520.11530773978690619No Hit
TTTCTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT23350.11447430799422872No Hit
ACTCGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT22200.10883638704376351No Hit
AGAATAGGGATGATTGGAAAAAAGATTTTAGGTTTATTTCCCCTCCATTT22190.10878736164419425No Hit
TTGGACGGGGGAATTCATACCTTTACAAATGCTTTAACAAGAGGAAATTG22160.10864028544548644No Hit
AGACCGGGGGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAA21580.10579681227046922No Hit
GGAAAAGGGATATATATTTTGGATATTATCTTGAAGCCTAATTAATTAAA21470.10525753287520731No Hit
TTTATTGGGGTGATTGTGAAAAGTACTTACTACATGTGGAAAGATAGTTT21240.10412994868511426No Hit
TGGAAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT21010.10300236449502123No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20830.10211990730277448No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGTC302.5286863E-644.032
TCGTTAG551.8189894E-1244.01
CAACGAA207.8579184E-444.023
CGGCATA254.4440563E-544.016
ATTACGC207.8579184E-444.035
TCTAGCG502.7284841E-1144.01
ATCGTAG254.4440563E-544.01
TACGCCC207.8579184E-444.040
ACTACGT254.4440563E-544.025
ACTACGG254.4440563E-544.02
CATCGAA254.4440563E-544.041
CCGGTCA207.8579184E-444.018
ATCGGCG207.8579184E-444.032
CGTCCAT207.8579184E-444.022
CAATCGA302.5286863E-644.021
GCCACTA502.7284841E-1144.011
TATCGGA207.8579184E-444.044
TGTCACG351.4468242E-743.9999961
CTACCGA700.043.99999639
TATAGCG700.043.9999961
Produced by FastQC (version 0.11.5)