##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546239_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2039759
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.878472407769742	31.0	31.0	34.0	30.0	34.0
2	32.23704172894935	33.0	31.0	34.0	30.0	34.0
3	32.16012872108911	33.0	31.0	34.0	30.0	34.0
4	35.89687311099007	37.0	35.0	37.0	35.0	37.0
5	35.886569933016595	37.0	35.0	37.0	35.0	37.0
6	35.95064073745967	37.0	35.0	37.0	35.0	37.0
7	36.18330155670351	37.0	35.0	37.0	35.0	37.0
8	36.07583739059369	37.0	35.0	37.0	35.0	37.0
9	37.85012837300877	39.0	38.0	39.0	35.0	39.0
10	37.48910189880275	39.0	37.0	39.0	35.0	39.0
11	37.27857114492448	39.0	37.0	39.0	34.0	39.0
12	36.64112181880311	39.0	35.0	39.0	33.0	39.0
13	36.40638722515748	39.0	35.0	39.0	33.0	39.0
14	37.46148000817744	40.0	35.0	41.0	33.0	41.0
15	37.67899099844639	40.0	35.0	41.0	33.0	41.0
16	37.79577881504629	40.0	35.0	41.0	33.0	41.0
17	37.716484643528965	40.0	35.0	41.0	33.0	41.0
18	37.55054984436887	39.0	36.0	41.0	33.0	41.0
19	37.32755095087214	38.0	35.0	41.0	33.0	41.0
20	37.07540106453752	38.0	35.0	41.0	33.0	41.0
21	36.95395828624852	38.0	35.0	41.0	33.0	41.0
22	36.84449388383628	38.0	35.0	41.0	33.0	41.0
23	36.82378947709019	38.0	35.0	41.0	33.0	41.0
24	36.72663976479574	38.0	35.0	41.0	33.0	41.0
25	36.7165488668024	38.0	35.0	41.0	33.0	41.0
26	36.66125409913622	38.0	35.0	41.0	33.0	41.0
27	36.57315741712624	38.0	35.0	41.0	32.0	41.0
28	36.507790381118554	38.0	35.0	41.0	32.0	41.0
29	36.41279729615116	38.0	35.0	41.0	32.0	41.0
30	36.17212915839567	37.0	35.0	41.0	31.0	41.0
31	35.81423099493617	37.0	35.0	40.0	30.0	41.0
32	35.457926156962664	37.0	35.0	41.0	29.0	41.0
33	35.063473675076324	37.0	35.0	41.0	25.0	41.0
34	34.636298209739486	37.0	34.0	40.0	23.0	41.0
35	34.39118640976704	37.0	34.0	41.0	21.0	41.0
36	34.25279457033895	37.0	34.0	40.0	20.0	41.0
37	34.1836903281221	37.0	34.0	40.0	19.0	41.0
38	34.13062327461235	37.0	34.0	40.0	18.0	41.0
39	34.05693270626579	37.0	34.0	40.0	18.0	41.0
40	33.87934604039007	36.0	34.0	40.0	18.0	41.0
41	33.77756784012229	36.0	34.0	40.0	18.0	41.0
42	33.68384598376573	36.0	33.0	40.0	17.0	41.0
43	33.55791738141614	36.0	33.0	40.0	16.0	41.0
44	33.450668436810425	36.0	33.0	40.0	15.0	41.0
45	33.403730048500826	35.0	33.0	40.0	16.0	41.0
46	33.34529275272226	35.0	33.0	40.0	16.0	41.0
47	33.28697360815665	35.0	33.0	40.0	15.0	41.0
48	33.21011501848993	35.0	33.0	40.0	15.0	41.0
49	33.163259483105605	36.0	33.0	40.0	15.0	41.0
50	32.99162891302355	35.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	3.0
12	4.0
13	8.0
14	17.0
15	46.0
16	127.0
17	324.0
18	819.0
19	1508.0
20	2962.0
21	4870.0
22	7648.0
23	11962.0
24	18559.0
25	31229.0
26	48302.0
27	59369.0
28	57249.0
29	50039.0
30	48227.0
31	51584.0
32	62405.0
33	81022.0
34	151592.0
35	298893.0
36	117309.0
37	145627.0
38	240043.0
39	548009.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.300079077969503	22.584089590976188	28.915768970746054	19.20006236030825
2	37.03427708861684	23.23465664326031	28.444830982483715	11.286235285639137
3	26.13490123097876	22.686748777674225	39.80847737404272	11.3698726173043
4	23.687700360679862	26.554215473494665	37.84849092466316	11.909593241162314
5	19.243842042123603	30.412808571993068	37.74906741433669	12.59428197154664
6	16.895770529753758	39.001470271733076	35.39040641565989	8.712352782853268
7	78.24870487150687	3.702202073872453	16.5339630809326	1.515129973688068
8	78.44877752714903	2.9276007606780996	16.02571676359805	2.597904948574807
9	75.00346854701952	3.867368645021299	18.155085968489416	2.974076839469761
10	43.1921124015141	23.22735185872449	23.770651336751058	9.809884403010356
11	36.20618906449242	20.61518051887502	31.336349049078837	11.842281367553715
12	33.20112817249489	19.924412638944112	33.92484112093634	12.949618067624655
13	18.908704410668122	32.85250855615786	34.81484822471674	13.423938808457272
14	13.553512939518836	34.93643121564852	37.63758365571619	13.872472189116458
15	11.006251228699076	23.49253024499463	52.469335838204415	13.031882688101879
16	13.95341312380531	19.75757920420991	49.55237358923284	16.736634082751934
17	13.387561961976878	19.102894018361972	38.24373369599056	29.26581032367059
18	16.500772885424208	23.53032882806253	43.33105038389339	16.637847902619868
19	19.903969047323727	25.116055377130337	36.57383053586232	18.40614503968361
20	21.554948403218223	21.969016927980217	38.15073251300766	18.325302155793896
21	17.255224759395595	25.964145764279017	39.6343391547727	17.146290321552694
22	18.415901094197892	21.381300437943896	35.39123984745257	24.811558620405645
23	14.70565885479608	25.27622135752312	35.32686949781812	24.691250289862676
24	13.761282582893372	22.550605243070382	48.39777640397714	15.29033577005911
25	14.834252477866258	23.811244367594405	43.82650107194036	17.528002082598974
26	13.747114242417855	27.389412180556626	38.63480930835457	20.228664268670958
27	13.455266038782032	31.457343735215776	36.51632374216758	18.57106648383461
28	12.462158519707476	28.932878835195723	41.22011472924007	17.384847915856728
29	13.482230008545127	25.442809665259475	39.77592450872873	21.29903581746667
30	15.34686205576247	28.683780779984303	37.707199723104544	18.262157441148684
31	20.5662531701049	28.378499616866502	33.99597697571134	17.05927023731725
32	21.282122054615275	25.55203825549979	35.67298881877712	17.492850871107812
33	20.55625198859277	26.367085523338783	32.88613998026237	20.19052250780607
34	16.864688426426845	26.74281618563762	33.29378617768079	23.098709210254743
35	16.601373005340335	24.26178778963593	36.34399946268162	22.79283974234211
36	24.605406815216895	25.852416878660666	32.19733311631423	17.344843189808206
37	16.738349971736856	29.694537442903794	35.86644304547743	17.700669539881915
38	17.1319258794789	29.13192195744693	32.35323388694449	21.38291827612968
39	16.864443299429002	28.88880500098296	33.63377732369363	20.612974375894407
40	19.10921829490641	25.06551019017443	32.91653572799532	22.90873578692385
41	15.268666543449497	26.314873472797522	34.12265860819832	24.29380137555466
42	18.837470505093982	26.478128053363164	32.28974599450229	22.394655447040556
43	18.74981309066414	24.04038908518114	34.38381691170378	22.825980912450934
44	15.877365904501465	28.434241496176753	33.26314530295	22.42524729637178
45	15.560514747085316	33.08979149007309	30.846732383580612	20.50296137926098
46	20.397066516191373	29.590897748214374	31.345124595601735	18.666911139992518
47	17.879269070512745	29.63835433499742	32.32215178361758	20.160224810872265
48	17.899222408137433	28.243434641053184	33.513125815353675	20.344217135455708
49	18.848109016800514	26.093965022338423	35.259655675008666	19.7982702858524
50	17.940992048570443	29.055197207121036	33.140581804026844	19.863228940281672
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3704.0
1	7453.0
2	11202.0
3	30627.0
4	50052.0
5	36673.0
6	23294.0
7	24426.5
8	25559.0
9	27735.5
10	29912.0
11	29623.0
12	29334.0
13	26836.0
14	24338.0
15	22222.0
16	20106.0
17	18727.5
18	17349.0
19	16867.5
20	16386.0
21	16894.0
22	17402.0
23	20620.5
24	23839.0
25	24831.5
26	25824.0
27	31112.0
28	36400.0
29	41911.0
30	47422.0
31	64900.0
32	82378.0
33	87517.0
34	92656.0
35	91309.0
36	89962.0
37	100327.5
38	110693.0
39	117943.0
40	125193.0
41	142572.5
42	159952.0
43	163924.5
44	167897.0
45	158768.0
46	149639.0
47	148252.0
48	146865.0
49	137118.5
50	127372.0
51	108251.5
52	89131.0
53	72317.5
54	55504.0
55	49728.5
56	43953.0
57	44043.0
58	44133.0
59	39220.5
60	34308.0
61	32685.0
62	31062.0
63	29864.5
64	28667.0
65	23505.0
66	18343.0
67	15998.5
68	13654.0
69	12104.5
70	10555.0
71	8710.0
72	6865.0
73	5599.5
74	4334.0
75	3341.5
76	2349.0
77	1694.5
78	1040.0
79	897.0
80	754.0
81	475.5
82	197.0
83	157.5
84	118.0
85	70.5
86	23.0
87	21.0
88	19.0
89	16.5
90	14.0
91	9.5
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2039759.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.795790838476027
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.10478546223639	17.023555674189474
2	8.876880817307221	3.8695727518421665
3	3.572826901111125	2.3361776351609564
4	1.904729999038743	1.6606038665127634
5	1.183289731878256	1.2895367748667423
6	0.8304824698316315	1.0860613324482729
7	0.5779646548358293	0.8818037710183607
8	0.4476326568553411	0.7805206209032263
9	0.34742849966409567	0.681523101900375
>10	2.742462373274853	12.760921199441938
>50	0.7702211270687088	12.114740778614657
>100	0.5389055234080087	22.06495882357601
>500	0.0647864559249319	9.609643980736463
>1k	0.037376801495153024	12.432405617594885
>5k	0.0	0.0
>10k+	2.2652606966759407E-4	1.407974071193794
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	28517	1.3980573195166683	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	4876	0.2390478482997256	No Hit
GAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCT	4037	0.1979155380611141	No Hit
GGGAAAGGGCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG	3171	0.15545954203413245	No Hit
TATTTTGGGTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAAT	2679	0.13133904544605515	No Hit
TTGAATGGGAGTGAAACAGACTATAAAAGTTTTCTTCCAATTTTTAGCTA	2672	0.1309958676490703	No Hit
GAAAAAGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATT	2418	0.11854341615847755	No Hit
GATGGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	2406	0.11795511136364639	No Hit
TACGAGAGGGACTGAATAAAATTTTCCAATCAAAATTCCTATAGTCTTAT	2405	0.11790608596407713	No Hit
TGATTAGGGATTCTTTTCCTGATCACTATGCATTTTGAACATTTTTTTAA	2352	0.11530773978690619	No Hit
TTTCTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	2335	0.11447430799422872	No Hit
ACTCGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	2220	0.10883638704376351	No Hit
AGAATAGGGATGATTGGAAAAAAGATTTTAGGTTTATTTCCCCTCCATTT	2219	0.10878736164419425	No Hit
TTGGACGGGGGAATTCATACCTTTACAAATGCTTTAACAAGAGGAAATTG	2216	0.10864028544548644	No Hit
AGACCGGGGGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAA	2158	0.10579681227046922	No Hit
GGAAAAGGGATATATATTTTGGATATTATCTTGAAGCCTAATTAATTAAA	2147	0.10525753287520731	No Hit
TTTATTGGGGTGATTGTGAAAAGTACTTACTACATGTGGAAAGATAGTTT	2124	0.10412994868511426	No Hit
TGGAAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	2101	0.10300236449502123	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2083	0.10211990730277448	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10030596751871176	0.0
2	0.0	0.0	0.0	0.38529061521483665	0.0
3	0.0	0.0	0.0	0.5457997734046032	0.0
4	0.0	0.0	0.0	0.8471589045568618	0.0
5	0.0	0.0	0.0	1.4622805929524028	0.0
6	0.0	0.0	0.0	2.051909073571927	0.0
7	0.0	0.0	0.0	2.418275884553028	0.0
8	0.0	0.0	0.0	3.3478464857858206	0.0
9	0.0	0.0	0.0	3.714605499963476	0.0
10	0.0	0.0	0.0	4.33953226827287	0.0
11	0.0	0.0	0.0	4.974215091096546	0.0
12	0.0	0.0	0.0	5.5554112029901574	0.0
13	0.0	0.0	0.0	5.752885512455148	0.0
14	0.0	0.0	0.0	5.819167852672791	0.0
15	4.902539956926284E-5	0.0	0.0	5.926680553928184	0.0
16	4.902539956926284E-5	0.0	0.0	6.200046181926394	0.0
17	4.902539956926284E-5	0.0	0.0	6.521995980897743	0.0
18	4.902539956926284E-5	0.0	0.0	7.088092269724021	0.0
19	4.902539956926284E-5	0.0	0.0	7.302039113444284	0.0
20	4.902539956926284E-5	0.0	0.0	7.540694758547456	0.0
21	4.902539956926284E-5	0.0	0.0	7.870586672249026	0.0
22	4.902539956926284E-5	0.0	0.0	8.19611532538893	0.0
23	4.902539956926284E-5	0.0	0.0	8.57263039408087	0.0
24	4.902539956926284E-5	0.0	0.0	8.86124292134512	0.0
25	4.902539956926284E-5	0.0	0.0	9.077346882646431	0.0
26	4.902539956926284E-5	0.0	0.0	9.333749722393675	0.0
27	4.902539956926284E-5	0.0	0.0	9.572013164300293	0.0
28	4.902539956926284E-5	0.0	0.0	9.832926340807909	0.0
29	4.902539956926284E-5	0.0	0.0	10.142325637489527	0.0
30	4.902539956926284E-5	0.0	0.0	10.554482171668319	0.0
31	4.902539956926284E-5	0.0	0.0	10.92482004001453	0.0
32	4.902539956926284E-5	0.0	0.0	11.230689507927162	0.0
33	4.902539956926284E-5	0.0	0.0	11.549501681326078	0.0
34	4.902539956926284E-5	0.0	0.0	11.852870853860678	0.0
35	4.902539956926284E-5	0.0	0.0	12.258359933698049	0.0
36	4.902539956926284E-5	0.0	0.0	12.569328043165884	0.0
37	4.902539956926284E-5	0.0	0.0	12.871422555311682	0.0
38	4.902539956926284E-5	0.0	0.0	13.208227050352518	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGAGTC	30	2.5286863E-6	44.0	32
TCGTTAG	55	1.8189894E-12	44.0	1
CAACGAA	20	7.8579184E-4	44.0	23
CGGCATA	25	4.4440563E-5	44.0	16
ATTACGC	20	7.8579184E-4	44.0	35
TCTAGCG	50	2.7284841E-11	44.0	1
ATCGTAG	25	4.4440563E-5	44.0	1
TACGCCC	20	7.8579184E-4	44.0	40
ACTACGT	25	4.4440563E-5	44.0	25
ACTACGG	25	4.4440563E-5	44.0	2
CATCGAA	25	4.4440563E-5	44.0	41
CCGGTCA	20	7.8579184E-4	44.0	18
ATCGGCG	20	7.8579184E-4	44.0	32
CGTCCAT	20	7.8579184E-4	44.0	22
CAATCGA	30	2.5286863E-6	44.0	21
GCCACTA	50	2.7284841E-11	44.0	11
TATCGGA	20	7.8579184E-4	44.0	44
TGTCACG	35	1.4468242E-7	43.999996	1
CTACCGA	70	0.0	43.999996	39
TATAGCG	70	0.0	43.999996	1
>>END_MODULE