FastQCFastQC Report
Thu 26 May 2016
SRR1546238_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546238_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2093444
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293541.4021870181385316No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC48150.23000376413221468No Hit
GAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCT41610.19876337747749642No Hit
GGGAAAGGGCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG31550.1507085931125934No Hit
TTGAATGGGAGTGAAACAGACTATAAAAGTTTTCTTCCAATTTTTAGCTA27400.1308848003576881No Hit
TATTTTGGGTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAAT26880.12840085524141082No Hit
TACGAGAGGGACTGAATAAAATTTTCCAATCAAAATTCCTATAGTCTTAT25690.12271644237916085No Hit
GATGGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT24490.11698426134159788No Hit
TGATTAGGGATTCTTTTCCTGATCACTATGCATTTTGAACATTTTTTTAA24410.11660211593909366No Hit
GAAAAAGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATT24260.11588559330939831No Hit
TTTATTGGGGTGATTGTGAAAAGTACTTACTACATGTGGAAAGATAGTTT23280.11120431212872187No Hit
TTTCTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT22630.10809938073337524No Hit
TTGGACGGGGGAATTCATACCTTTACAAATGCTTTAACAAGAGGAAATTG22530.107621698980245No Hit
ACTCGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT22280.10642749459741938No Hit
AGACCGGGGGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAA22140.10575874014303703No Hit
AGAATAGGGATGATTGGAAAAAAGATTTTAGGTTTATTTCCCCTCCATTT21700.10365694042926393No Hit
TGGAAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT21430.10236719969581227No Hit
AGTTCTGGGGACAGGTTGAGGTTTATTTAAGTAAAATGATTTTTTAAAAA21360.10203282246862108No Hit
GGAAAAGGGATATATATTTTGGATATTATCTTGAAGCCTAATTAATTAAA21340.10193728611799505No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTGA207.8579644E-444.00000415
CCAGCGA207.8579644E-444.00000435
CGACAGT207.8579644E-444.00000413
ATCGTCC207.8579644E-444.00000416
CTAACCG207.8579644E-444.0000041
GCCGAAT207.8579644E-444.00000429
TCGTCGT207.8579644E-444.00000421
TAATGCG454.802132E-1044.0000041
CCCGATA207.8579644E-444.00000437
GGTACGA254.444098E-544.015
TCGATTG351.4468424E-744.01
ATCGTAG254.444098E-544.01
ACGATAT551.8189894E-1244.016
ACTACGT254.444098E-544.025
TCGTACA1200.044.034
TTACGTA351.4468424E-744.023
TTACGAA254.444098E-544.019
CGGGATA4050.043.456796
GCGTAAG2250.043.0222241
AGTACGG1750.042.7428552
Produced by FastQC (version 0.11.5)