##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546238_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2093444
Sequences flagged as poor quality	0
Sequence length	50
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.813672111601743	31.0	31.0	34.0	30.0	34.0
2	32.20675164943509	33.0	31.0	34.0	30.0	34.0
3	32.24654970469714	34.0	31.0	34.0	30.0	34.0
4	35.930731846660336	37.0	35.0	37.0	35.0	37.0
5	35.84840674028061	37.0	35.0	37.0	35.0	37.0
6	35.93392132772598	37.0	35.0	37.0	35.0	37.0
7	36.1627347089294	37.0	35.0	37.0	35.0	37.0
8	36.059805277810156	37.0	35.0	37.0	35.0	37.0
9	37.82227086083984	39.0	38.0	39.0	35.0	39.0
10	37.47941764862112	39.0	37.0	39.0	35.0	39.0
11	37.269463143031295	39.0	37.0	39.0	34.0	39.0
12	36.62542967473694	39.0	35.0	39.0	33.0	39.0
13	36.34880847063499	39.0	35.0	39.0	33.0	39.0
14	37.38968178752334	40.0	35.0	41.0	33.0	41.0
15	37.6433766558838	40.0	35.0	41.0	33.0	41.0
16	37.749001645135955	40.0	35.0	41.0	33.0	41.0
17	37.67766226371472	40.0	35.0	41.0	33.0	41.0
18	37.53016703575543	39.0	36.0	41.0	33.0	41.0
19	37.3128949233894	38.0	35.0	41.0	33.0	41.0
20	37.0635440928919	38.0	35.0	41.0	33.0	41.0
21	36.93979108110845	38.0	35.0	41.0	33.0	41.0
22	36.83682821226648	38.0	35.0	41.0	33.0	41.0
23	36.80286074048315	38.0	35.0	41.0	33.0	41.0
24	36.709011561809156	38.0	35.0	41.0	33.0	41.0
25	36.68791188109164	38.0	35.0	41.0	33.0	41.0
26	36.65050510068576	38.0	35.0	41.0	33.0	41.0
27	36.55637552282268	38.0	35.0	41.0	32.0	41.0
28	36.486866617879436	38.0	35.0	41.0	32.0	41.0
29	36.39307667174283	38.0	35.0	41.0	32.0	41.0
30	36.15895146944461	37.0	35.0	40.0	31.0	41.0
31	35.80830679015058	37.0	35.0	40.0	30.0	41.0
32	35.455825902197525	37.0	35.0	41.0	29.0	41.0
33	35.0567982711742	37.0	35.0	41.0	25.0	41.0
34	34.692095417885554	37.0	34.0	41.0	23.0	41.0
35	34.41492679049451	37.0	34.0	41.0	21.0	41.0
36	34.265375142588006	37.0	34.0	40.0	20.0	41.0
37	34.13008086196717	37.0	34.0	40.0	18.0	41.0
38	34.08911774090924	37.0	34.0	40.0	18.0	41.0
39	34.02404506640732	37.0	34.0	40.0	18.0	41.0
40	33.85856368739742	36.0	34.0	40.0	18.0	41.0
41	33.783795984033965	36.0	34.0	40.0	18.0	41.0
42	33.67794266290381	36.0	33.0	40.0	17.0	41.0
43	33.58023620407329	36.0	33.0	40.0	16.0	41.0
44	33.47150150660825	36.0	33.0	40.0	15.0	41.0
45	33.41906829129415	35.0	33.0	40.0	17.0	41.0
46	33.37542346487415	35.0	33.0	40.0	17.0	41.0
47	33.29907845636186	35.0	33.0	40.0	15.0	41.0
48	33.23537911690019	35.0	33.0	40.0	15.0	41.0
49	33.18607853852312	36.0	33.0	40.0	15.0	41.0
50	32.99389140574097	35.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	4.0
11	4.0
12	3.0
13	11.0
14	25.0
15	50.0
16	127.0
17	337.0
18	860.0
19	1636.0
20	3047.0
21	5005.0
22	7969.0
23	12154.0
24	19181.0
25	31652.0
26	48660.0
27	60946.0
28	58938.0
29	52118.0
30	50113.0
31	53739.0
32	64150.0
33	84573.0
34	158725.0
35	302124.0
36	120836.0
37	150917.0
38	247507.0
39	558032.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	29.4559586977249	22.59883713154018	28.959265210820067	18.985938959914858
2	37.110140037182745	23.226033273400194	28.36225855575788	11.301568133659176
3	26.124797224095797	22.718878556101814	39.75821660383559	11.398107615966799
4	23.79394910969675	26.525142301394254	37.76150687575115	11.91940171315784
5	19.304695993778672	30.47485387715172	37.561883671118025	12.658566457951586
6	16.89980720764444	38.95308400893456	35.38819285349883	8.758915929922177
7	78.27288429974722	3.718847984469611	16.460435531115234	1.5478321846679444
8	78.47140883634815	2.9391758270104194	15.96909207984546	2.620323256795978
9	75.00119420438283	3.89286744713496	18.12453545449508	2.9814028939871333
10	43.214912842187324	23.194888423096106	23.74341038021557	9.846788354501005
11	36.202783547111835	20.595009945334102	31.295224519977605	11.906981987576453
12	33.24488259537872	19.863105963187934	33.90924237763227	12.982769063801086
13	18.98675101889518	32.74322121824133	34.85252053553857	13.417507227324924
14	13.571511824534117	34.914237018042996	37.70332523821989	13.810925919203
15	11.099843129312271	23.41906446984013	52.42609785597322	13.05499454487438
16	14.023733140222525	19.725151472883915	49.49919845001824	16.75191693687531
17	13.42405146734281	19.07808377009368	38.191515989919004	29.3063487726445
18	16.664166798825285	23.416341683847286	43.237984870863514	16.681506646463912
19	19.919997859985745	25.107908308032123	36.61874881773766	18.35334501424447
20	21.662246518177703	21.941499271057644	38.032925647879765	18.36332856288489
21	17.261555599289974	25.97470961726227	39.62212507236879	17.141609711078967
22	18.35511243673105	21.381942865440873	35.446661100081975	24.816283597746107
23	14.740542378969772	25.220259056368356	35.29585697061875	24.743341594043116
24	13.806770087950765	22.504160608069764	48.33370274055576	15.355366563423717
25	14.886951836304195	23.741451885027733	43.71867601903848	17.652920259629585
26	13.730341007449926	27.430444759926704	38.59071463100995	20.248499601613418
27	13.489302794820402	31.41985168936929	36.504582878739534	18.58626263707078
28	12.527012903139514	28.76121835597226	41.19904807580237	17.51272066508586
29	13.563486771081529	25.370633272253762	39.71364889626854	21.35223106039617
30	15.408245933495234	28.613137012501888	37.73427901582273	18.244338038180146
31	20.61712661050403	28.243841249156894	33.99183355274849	17.14719858759059
32	21.243462925208412	25.543267457835032	35.651395499473594	17.56187411748296
33	20.544423447677605	26.39678921432816	32.81716635362589	20.241620984368343
34	16.830352280739298	26.769333213594443	33.20939084112114	23.190923664545124
35	16.592944449433563	24.29422520975006	36.20378667879341	22.909043662022963
36	24.605769249141606	25.82543406940907	32.13164526970867	17.437151411740654
37	16.720867622921844	29.70373222307356	35.82503281673644	17.75036733726816
38	17.223006681812365	29.06468957373591	32.32267020278546	21.38963354166627
39	16.88375710073926	28.811231635525004	33.584753162730884	20.720258101004852
40	19.160913786086468	25.08230456606434	32.81544669931462	22.941334948534568
41	15.336068220597255	26.199649954811306	34.14536046820455	24.31892135638689
42	18.925559986319197	26.417663906939953	32.315266135611935	22.341509971128914
43	18.784261723743267	23.949625593041894	34.38143079060151	22.88468189261332
44	15.910719369612943	28.36178087400475	33.188659453035285	22.538840303347023
45	15.57051442503358	33.04124686401929	30.845439381230165	20.542799329716964
46	20.40455823036107	29.47855304464796	31.286148566668132	18.83074015832284
47	17.83190761252749	29.637525532089704	32.263246592696056	20.26732026268675
48	17.85545732295681	28.12308330196556	33.60429034643391	20.41716902864371
49	18.89326869980759	25.979820812020765	35.25654376233613	19.870366725835513
50	17.894483922187554	28.91321668981831	33.186223276094324	20.006076111899816
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3751.0
1	7655.5
2	11560.0
3	31466.5
4	51373.0
5	37164.0
6	22955.0
7	24493.0
8	26031.0
9	27844.0
10	29657.0
11	29803.0
12	29949.0
13	27450.5
14	24952.0
15	22700.0
16	20448.0
17	19172.0
18	17896.0
19	17355.5
20	16815.0
21	17430.5
22	18046.0
23	21213.5
24	24381.0
25	25410.0
26	26439.0
27	31711.5
28	36984.0
29	42725.0
30	48466.0
31	66329.0
32	84192.0
33	88562.5
34	92933.0
35	92709.0
36	92485.0
37	102338.0
38	112191.0
39	120005.5
40	127820.0
41	145772.5
42	163725.0
43	168295.0
44	172865.0
45	164130.5
46	155396.0
47	154232.5
48	153069.0
49	142649.0
50	132229.0
51	112767.0
52	93305.0
53	75661.0
54	58017.0
55	51546.0
56	45075.0
57	45327.5
58	45580.0
59	40114.0
60	34648.0
61	33138.5
62	31629.0
63	30379.5
64	29130.0
65	23904.5
66	18679.0
67	16288.0
68	13897.0
69	12320.5
70	10744.0
71	8814.0
72	6884.0
73	5667.0
74	4450.0
75	3450.5
76	2451.0
77	1789.0
78	1127.0
79	966.5
80	806.0
81	519.5
82	233.0
83	169.0
84	105.0
85	64.0
86	23.0
87	26.5
88	30.0
89	21.5
90	13.0
91	9.5
92	6.0
93	4.5
94	3.0
95	2.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2093444.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.847056467673628
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.21002035992926	17.08658731141279
2	8.71007288599386	3.805789083557217
3	3.5573619262378355	2.3315366063541063
4	1.9105093057978064	1.669560187431225
5	1.2287827511584277	1.3422643075530762
6	0.8228007135291591	1.0785464190068215
7	0.6003093356297831	0.9180494367502858
8	0.4429569467179152	0.7741844342155674
9	0.3637136316298474	0.7151465023451928
>10	2.7504025846338003	12.70093909561663
>50	0.7587591480592564	11.952605106502988
>100	0.5431340592630644	22.242361644863585
>500	0.06370362867184796	9.495641819910768
>1k	0.03725229496727441	12.47318800197234
>5k	0.0	0.0
>10k+	2.2042778087144618E-4	1.413600042507446
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29354	1.4021870181385316	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	4815	0.23000376413221468	No Hit
GAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCT	4161	0.19876337747749642	No Hit
GGGAAAGGGCCAGCACTTAGATATTTTAAAGAGGCATCTATCACATAAGG	3155	0.1507085931125934	No Hit
TTGAATGGGAGTGAAACAGACTATAAAAGTTTTCTTCCAATTTTTAGCTA	2740	0.1308848003576881	No Hit
TATTTTGGGTCCTTATAAACTACTGCTAAGACAGCTAAGAAAGCTCCAAT	2688	0.12840085524141082	No Hit
TACGAGAGGGACTGAATAAAATTTTCCAATCAAAATTCCTATAGTCTTAT	2569	0.12271644237916085	No Hit
GATGGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	2449	0.11698426134159788	No Hit
TGATTAGGGATTCTTTTCCTGATCACTATGCATTTTGAACATTTTTTTAA	2441	0.11660211593909366	No Hit
GAAAAAGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATT	2426	0.11588559330939831	No Hit
TTTATTGGGGTGATTGTGAAAAGTACTTACTACATGTGGAAAGATAGTTT	2328	0.11120431212872187	No Hit
TTTCTTGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	2263	0.10809938073337524	No Hit
TTGGACGGGGGAATTCATACCTTTACAAATGCTTTAACAAGAGGAAATTG	2253	0.107621698980245	No Hit
ACTCGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	2228	0.10642749459741938	No Hit
AGACCGGGGGGATATATTCCTTATAAACTACTGCTAAGACAGCTAAGAAA	2214	0.10575874014303703	No Hit
AGAATAGGGATGATTGGAAAAAAGATTTTAGGTTTATTTCCCCTCCATTT	2170	0.10365694042926393	No Hit
TGGAAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	2143	0.10236719969581227	No Hit
AGTTCTGGGGACAGGTTGAGGTTTATTTAAGTAAAATGATTTTTTAAAAA	2136	0.10203282246862108	No Hit
GGAAAAGGGATATATATTTTGGATATTATCTTGAAGCCTAATTAATTAAA	2134	0.10193728611799505	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10470784028615047	0.0
2	0.0	0.0	0.0	0.38582355200330176	0.0
3	0.0	0.0	0.0	0.5495250888010379	0.0
4	0.0	0.0	0.0	0.8545726563500147	0.0
5	0.0	0.0	0.0	1.4661963730579848	0.0
6	0.0	0.0	0.0	2.0734731858124698	0.0
7	0.0	0.0	0.0	2.453182411375704	0.0
8	0.0	0.0	0.0	3.400807473235491	0.0
9	0.0	0.0	0.0	3.779131421714648	0.0
10	0.0	0.0	0.0	4.427918778816152	0.0
11	0.0	0.0	0.0	5.093281692751275	0.0
12	0.0	0.0	0.0	5.692581220228485	0.0
13	0.0	0.0	0.0	5.897697765022613	0.0
14	0.0	0.0	0.0	5.964859819512727	0.0
15	0.0	0.0	0.0	6.081318630925881	0.0
16	0.0	0.0	0.0	6.371462527777194	0.0
17	0.0	0.0	0.0	6.7064607412474375	0.0
18	0.0	0.0	0.0	7.291811961533244	0.0
19	0.0	0.0	0.0	7.515510326524139	0.0
20	0.0	0.0	0.0	7.757360598133984	0.0
21	0.0	0.0	0.0	8.093600784162366	0.0
22	0.0	0.0	0.0	8.437053964663015	0.0
23	0.0	0.0	0.0	8.84852902680941	0.0
24	0.0	0.0	0.0	9.159404311746577	0.0
25	0.0	0.0	0.0	9.386924130762514	0.0
26	0.0	0.0	0.0	9.65437814434014	0.0
27	0.0	0.0	0.0	9.917533022139594	0.0
28	0.0	0.0	0.0	10.192295566540112	0.0
29	0.0	0.0	0.0	10.51740576772056	0.0
30	0.0	0.0	0.0	10.937574637773926	0.0
31	0.0	0.0	0.0	11.328127239133218	0.0
32	0.0	0.0	0.0	11.644257023354816	0.0
33	0.0	0.0	0.0	11.970513660742776	0.0
34	0.0	0.0	0.0	12.295146180170093	0.0
35	0.0	0.0	0.0	12.711971277951548	0.0
36	0.0	0.0	0.0	13.037845769937004	0.0
37	0.0	0.0	0.0	13.350918390938569	0.0
38	0.0	0.0	0.0	13.703304220222753	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTGA	20	7.8579644E-4	44.000004	15
CCAGCGA	20	7.8579644E-4	44.000004	35
CGACAGT	20	7.8579644E-4	44.000004	13
ATCGTCC	20	7.8579644E-4	44.000004	16
CTAACCG	20	7.8579644E-4	44.000004	1
GCCGAAT	20	7.8579644E-4	44.000004	29
TCGTCGT	20	7.8579644E-4	44.000004	21
TAATGCG	45	4.802132E-10	44.000004	1
CCCGATA	20	7.8579644E-4	44.000004	37
GGTACGA	25	4.444098E-5	44.0	15
TCGATTG	35	1.4468424E-7	44.0	1
ATCGTAG	25	4.444098E-5	44.0	1
ACGATAT	55	1.8189894E-12	44.0	16
ACTACGT	25	4.444098E-5	44.0	25
TCGTACA	120	0.0	44.0	34
TTACGTA	35	1.4468424E-7	44.0	23
TTACGAA	25	4.444098E-5	44.0	19
CGGGATA	405	0.0	43.45679	6
GCGTAAG	225	0.0	43.022224	1
AGTACGG	175	0.0	42.742855	2
>>END_MODULE