FastQCFastQC Report
Thu 26 May 2016
SRR1546237_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546237_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2887999
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT421881.4608038299182236No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC59320.2054017331723453No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA38260.1324792702490548No Hit
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC35800.12396126175943965No Hit
GAGTACGGGTAGCTTTGGGTTATGAAACTCCATGATTTTCATTTAATTTT33310.11533937511751216No Hit
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTG32670.11312330786818141No Hit
ATGTTTGGGTCTTACAAATATTTAAAGGCCTTAAACGTTGATTTACCTTT32220.1115651355834957No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGC29650.10266624053540184No Hit
TATTTAGGGAGGACTTATTCTTCTTACATGACAAAAAATTGCTCCCCTAT29240.10124657245379934No Hit
GGAGGAAGGCTGCTTTCTGTGAAATTTTCTTTCTATTTTTCTATTTTTAG29130.1008656858953206No Hit
TTTTGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT29090.10072718169223743No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAACGA302.529052E-644.00000411
CTACGGC650.044.00000412
GTTGCGT302.529052E-644.0000049
AATCCCG254.444518E-544.01
CGAACTT254.444518E-544.029
AAATCGA207.8584626E-444.037
ACCGGAT351.447097E-744.017
ATTACGC351.447097E-744.012
ATTACGA408.3200575E-944.030
CATTACG408.3200575E-944.029
AACGGCG254.444518E-544.013
CTTGCGG1800.044.02
TTGACGA207.8584626E-444.022
AACGCAT502.7284841E-1144.010
CGTCGGT207.8584626E-444.012
TCGGTAC207.8584626E-444.036
TACGTTG1800.044.01
ACGCGTA1100.044.023
CGGACGT254.444518E-544.035
CGACGGT1700.043.99999628
Produced by FastQC (version 0.11.5)