##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546237_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2887999
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.873559166744865	31.0	31.0	34.0	30.0	34.0
2	32.227972724367284	33.0	31.0	34.0	30.0	34.0
3	32.11472441645582	33.0	31.0	34.0	30.0	34.0
4	35.87029912406479	37.0	35.0	37.0	35.0	37.0
5	35.84868104178707	37.0	35.0	37.0	35.0	37.0
6	35.91706783831989	37.0	35.0	37.0	35.0	37.0
7	36.18358628240522	37.0	35.0	37.0	35.0	37.0
8	36.07652703480853	37.0	35.0	37.0	35.0	37.0
9	37.817406100209865	39.0	38.0	39.0	35.0	39.0
10	37.45683776206294	39.0	37.0	39.0	35.0	39.0
11	37.282332853993374	39.0	37.0	39.0	34.0	39.0
12	36.741065007293976	39.0	35.0	39.0	33.0	39.0
13	36.52675676134237	39.0	35.0	39.0	33.0	39.0
14	37.60172285378215	40.0	35.0	41.0	33.0	41.0
15	37.787023471961035	40.0	35.0	41.0	33.0	41.0
16	37.934043952231285	40.0	35.0	41.0	33.0	41.0
17	37.84887183132681	40.0	35.0	41.0	33.0	41.0
18	37.6524472480773	39.0	36.0	41.0	33.0	41.0
19	37.429866492336046	38.0	36.0	41.0	33.0	41.0
20	37.14371507746367	38.0	35.0	41.0	33.0	41.0
21	37.045068921422754	38.0	35.0	41.0	33.0	41.0
22	36.94819007901319	38.0	35.0	41.0	33.0	41.0
23	36.89969352482463	38.0	35.0	41.0	33.0	41.0
24	36.86128146166256	38.0	35.0	41.0	33.0	41.0
25	36.84901206683244	38.0	35.0	41.0	33.0	41.0
26	36.79971218826599	38.0	35.0	41.0	33.0	41.0
27	36.730981208788506	38.0	35.0	41.0	33.0	41.0
28	36.65560929903369	38.0	35.0	41.0	32.0	41.0
29	36.53479589154983	38.0	35.0	41.0	32.0	41.0
30	36.30408736291114	38.0	35.0	41.0	31.0	41.0
31	35.977326861955284	38.0	35.0	41.0	30.0	41.0
32	35.592499512638334	38.0	35.0	41.0	29.0	41.0
33	35.179130255931526	38.0	35.0	41.0	25.0	41.0
34	34.675853419616836	38.0	34.0	41.0	22.0	41.0
35	34.401840859363176	38.0	34.0	41.0	20.0	41.0
36	34.26754337518815	38.0	34.0	40.0	18.0	41.0
37	34.20924799489197	38.0	34.0	40.0	18.0	41.0
38	34.129628507489095	37.0	34.0	40.0	18.0	41.0
39	34.05811809491624	37.0	34.0	40.0	18.0	41.0
40	33.94424547930937	37.0	34.0	40.0	18.0	41.0
41	33.8507579123123	37.0	34.0	40.0	17.0	41.0
42	33.802575070143725	37.0	34.0	40.0	17.0	41.0
43	33.686853077165196	37.0	33.0	40.0	15.0	41.0
44	33.55730836471896	37.0	33.0	40.0	15.0	41.0
45	33.504288955778726	36.0	33.0	40.0	15.0	41.0
46	33.435607837814345	36.0	33.0	40.0	15.0	41.0
47	33.392819388095354	36.0	33.0	40.0	15.0	41.0
48	33.32254200919045	36.0	33.0	40.0	15.0	41.0
49	33.251267746283844	36.0	33.0	40.0	15.0	41.0
50	33.08699933760365	36.0	33.0	40.0	13.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	4.0
11	3.0
12	4.0
13	7.0
14	20.0
15	61.0
16	158.0
17	427.0
18	991.0
19	2146.0
20	3959.0
21	6482.0
22	10307.0
23	16442.0
24	26394.0
25	44973.0
26	69972.0
27	85455.0
28	82958.0
29	71258.0
30	68758.0
31	73351.0
32	86480.0
33	111045.0
34	202813.0
35	388554.0
36	159883.0
37	200403.0
38	349885.0
39	824801.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.864830631866564	22.72085274267754	26.712855509991517	19.701461115464376
2	38.21590658445519	23.198899999619112	28.193950205661427	10.39124321026427
3	27.574836417879645	23.14903848650917	38.30541492569769	10.970710169913493
4	24.24592252282636	24.493325655583675	38.729480169487594	12.531271652102372
5	19.95738225671131	30.013029782905047	38.164036760400535	11.865551199983102
6	17.184389606783103	39.47889178631987	35.52092642691358	7.815792179983442
7	77.76834410261222	3.7269749747143264	16.71132850115253	1.7933524215209213
8	77.67810861430353	3.399793420981102	16.44633533460365	2.475762630111714
9	73.63392438847798	4.598235664209025	18.62642611718356	3.141413830129443
10	39.562894585489815	25.970957746176502	24.270714775178245	10.195432893155434
11	33.09398652838868	21.485083616718704	32.76507367211693	12.655856182775688
12	31.956555386618902	19.874833751673737	36.59686170251444	11.571749159192922
13	18.89682094765268	31.946686962149222	35.49409123756622	13.662400852631873
14	12.933106971297429	34.768121457105764	38.031038099389924	14.267733472206881
15	10.597579846807426	23.94211355336342	52.761583366199226	12.698723233629927
16	12.808106928014865	20.318324209946056	49.91916548447558	16.954403377563494
17	13.799069875024195	21.084148574843688	37.380830118015965	27.735951432116146
18	15.382276794417171	24.654960060581736	43.623768567786904	16.338994577214187
19	19.53321313476909	24.789032129166248	37.317360566953106	18.360394169111554
20	20.631378334964797	22.240347036131244	38.970061970243066	18.158212658660894
21	17.50333016043288	26.124662785548058	40.62671074332089	15.745296310698167
22	18.406585320839792	22.19744535922623	36.272588737045965	23.123380582888014
23	13.922511746022073	26.68484303491795	36.80818449036859	22.58446072869139
24	13.896299825588581	23.846857287692966	46.918264168374016	15.338578718344431
25	14.40824598623476	26.55222526046581	41.8133108771852	17.226217876114223
26	13.499693040059919	27.597689611388372	38.455345725535224	20.447271623016487
27	13.602844045306108	33.00510145606006	35.896445947522835	17.495608551110994
28	12.547822904370811	28.52009297787153	40.72671077794695	18.205373339810713
29	13.419118219916282	24.549939248593923	40.19097652042124	21.83996601106856
30	14.704783485035833	28.57123565485999	39.09277669417476	17.63120416592942
31	19.503780991613915	28.921097271848083	34.89592621050077	16.679195526037233
32	20.07220224106726	25.174731708702115	36.848142952958085	17.90492309727254
33	21.551150121589377	27.25776567097149	33.31292704741241	17.878157160026717
34	17.128884047397523	26.186401034072382	34.95066999676939	21.734044921760706
35	16.316626148416255	26.452779242652092	36.0648324324212	21.165762176510448
36	23.885326830099316	26.814379090851485	33.074007297093935	16.226286781955256
37	16.64418858870796	30.08207412814201	37.53848252717539	15.73525475597464
38	16.710739858289426	29.8140338691253	34.65468651478065	18.820539757804625
39	16.304299274341854	28.66427585328111	35.87386976242028	19.157555109956757
40	19.303400035803335	25.63338837721204	34.39194404153187	20.67126754545275
41	14.54872387421187	26.585431643155	35.282837701813605	23.583006780819524
42	16.714721854128065	27.54827823693845	32.99242832147795	22.744571587455535
43	17.490172261139982	25.095992069249327	35.10981132611196	22.304024343498735
44	16.622581933026986	26.951706008208454	34.16711709387711	22.258594964887454
45	16.114410011914824	32.71947116325179	31.798452838799456	19.367665986033927
46	19.736675809098273	29.866249953687657	32.56559299362639	17.83148124358769
47	16.975940781142928	28.897066792613156	34.34024734773108	19.786745078512837
48	16.7743132875046	28.508077738254062	34.973730946582734	19.743878027658596
49	18.90672399817313	26.59097181127833	35.45056629174733	19.05173789880121
50	18.089653078134724	27.75045974738911	33.808495086044005	20.351392088432164
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5582.0
1	9614.0
2	13646.0
3	44715.5
4	75785.0
5	54689.0
6	33593.0
7	36165.5
8	38738.0
9	40934.0
10	43130.0
11	42360.5
12	41591.0
13	39295.0
14	36999.0
15	34899.0
16	32799.0
17	30112.5
18	27426.0
19	25104.0
20	22782.0
21	24808.5
22	26835.0
23	30338.5
24	33842.0
25	33398.0
26	32954.0
27	42889.5
28	52825.0
29	62590.5
30	72356.0
31	91191.0
32	110026.0
33	126183.5
34	142341.0
35	158621.0
36	174901.0
37	169885.5
38	164870.0
39	178824.0
40	192778.0
41	207068.5
42	221359.0
43	231330.0
44	241301.0
45	224187.0
46	207073.0
47	201573.0
48	196073.0
49	186001.0
50	175929.0
51	145564.0
52	115199.0
53	99227.0
54	83255.0
55	72347.5
56	61440.0
57	55716.5
58	49993.0
59	47203.5
60	44414.0
61	40969.5
62	37525.0
63	32828.0
64	28131.0
65	22798.0
66	17465.0
67	14915.5
68	12366.0
69	10192.5
70	8019.0
71	6804.0
72	5589.0
73	4338.5
74	3088.0
75	2632.0
76	2176.0
77	1524.0
78	872.0
79	656.0
80	440.0
81	366.0
82	292.0
83	227.0
84	162.0
85	92.5
86	23.0
87	15.0
88	7.0
89	6.5
90	6.0
91	3.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2887999.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.072024549512054
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.16373097967477	15.488323025559758
2	9.190106579665837	3.689280897593699
3	3.712466168234442	2.23550136204104
4	2.031514270395604	1.6310641723225854
5	1.3362656905130232	1.3410778872324043
6	0.9234990887826721	1.1121897828898684
7	0.6519226682642251	0.915978546124805
8	0.4948464982592683	0.7946056849040086
9	0.3830580086602724	0.691987477834457
>10	2.7739559980625	11.392496730486004
>50	0.5925454317632561	8.634716775277333
>100	0.6226978460122659	23.909210806049654
>500	0.07135432496738785	10.074415742481182
>1k	0.05168837686661997	16.40820711677545
>5k	1.7403493894484838E-4	0.2072186151928875
>10k+	1.7403493894484838E-4	1.4737253772349188
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	42188	1.4608038299182236	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	5932	0.2054017331723453	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3826	0.1324792702490548	No Hit
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	3580	0.12396126175943965	No Hit
GAGTACGGGTAGCTTTGGGTTATGAAACTCCATGATTTTCATTTAATTTT	3331	0.11533937511751216	No Hit
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTG	3267	0.11312330786818141	No Hit
ATGTTTGGGTCTTACAAATATTTAAAGGCCTTAAACGTTGATTTACCTTT	3222	0.1115651355834957	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGC	2965	0.10266624053540184	No Hit
TATTTAGGGAGGACTTATTCTTCTTACATGACAAAAAATTGCTCCCCTAT	2924	0.10124657245379934	No Hit
GGAGGAAGGCTGCTTTCTGTGAAATTTTCTTTCTATTTTTCTATTTTTAG	2913	0.1008656858953206	No Hit
TTTTGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	2909	0.10072718169223743	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14380198885110418	0.0
2	0.0	0.0	0.0	0.5022508664303554	0.0
3	0.0	0.0	0.0	0.6951179692236735	0.0
4	0.0	0.0	0.0	1.0132621237057216	0.0
5	0.0	0.0	0.0	1.6856654036237546	0.0
6	0.0	0.0	0.0	2.2396129638549045	0.0
7	0.0	0.0	0.0	2.6007280473435066	0.0
8	0.0	0.0	0.0	3.463851614907069	0.0
9	0.0	0.0	0.0	3.786116269430841	0.0
10	3.4626050770793206E-5	0.0	0.0	4.437743918886398	0.0
11	3.4626050770793206E-5	0.0	0.0	5.1004865306393805	0.0
12	3.4626050770793206E-5	0.0	0.0	5.6831390869595175	0.0
13	3.4626050770793206E-5	0.0	0.0	5.890756887381194	0.0
14	3.4626050770793206E-5	0.0	0.0	5.968180736904687	0.0
15	3.4626050770793206E-5	0.0	0.0	6.091622607902565	0.0
16	6.925210154158641E-5	0.0	0.0	6.388506367211346	0.0
17	6.925210154158641E-5	0.0	0.0	6.738160227894816	0.0
18	6.925210154158641E-5	0.0	0.0	7.296089783964606	0.0
19	6.925210154158641E-5	0.0	0.0	7.536221446060058	0.0
20	6.925210154158641E-5	0.0	0.0	7.796782478110276	0.0
21	6.925210154158641E-5	0.0	0.0	8.146955729555309	0.0
22	6.925210154158641E-5	0.0	0.0	8.499241170097358	0.0
23	6.925210154158641E-5	0.0	0.0	8.926492010558176	0.0
24	6.925210154158641E-5	0.0	0.0	9.245259433954098	0.0
25	6.925210154158641E-5	0.0	0.0	9.49854899534245	0.0
26	6.925210154158641E-5	0.0	0.0	9.787399510872406	0.0
27	6.925210154158641E-5	0.0	0.0	10.064858055698773	0.0
28	6.925210154158641E-5	0.0	0.0	10.33937338620962	0.0
29	6.925210154158641E-5	0.0	0.0	10.637780691752317	0.0
30	6.925210154158641E-5	0.0	0.0	11.044809918562992	0.0
31	6.925210154158641E-5	0.0	0.0	11.438681246080764	0.0
32	6.925210154158641E-5	0.0	0.0	11.763646732564657	0.0
33	6.925210154158641E-5	0.0	0.0	12.089270114013198	0.0
34	6.925210154158641E-5	0.0	0.0	12.419325629960397	0.0
35	6.925210154158641E-5	0.0	0.0	12.875904735424077	0.0
36	6.925210154158641E-5	0.0	0.0	13.199381301724827	0.0
37	6.925210154158641E-5	0.0	0.0	13.519256758745415	0.0
38	6.925210154158641E-5	0.0	0.0	13.862435547934746	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCAACGA	30	2.529052E-6	44.000004	11
CTACGGC	65	0.0	44.000004	12
GTTGCGT	30	2.529052E-6	44.000004	9
AATCCCG	25	4.444518E-5	44.0	1
CGAACTT	25	4.444518E-5	44.0	29
AAATCGA	20	7.8584626E-4	44.0	37
ACCGGAT	35	1.447097E-7	44.0	17
ATTACGC	35	1.447097E-7	44.0	12
ATTACGA	40	8.3200575E-9	44.0	30
CATTACG	40	8.3200575E-9	44.0	29
AACGGCG	25	4.444518E-5	44.0	13
CTTGCGG	180	0.0	44.0	2
TTGACGA	20	7.8584626E-4	44.0	22
AACGCAT	50	2.7284841E-11	44.0	10
CGTCGGT	20	7.8584626E-4	44.0	12
TCGGTAC	20	7.8584626E-4	44.0	36
TACGTTG	180	0.0	44.0	1
ACGCGTA	110	0.0	44.0	23
CGGACGT	25	4.444518E-5	44.0	35
CGACGGT	170	0.0	43.999996	28
>>END_MODULE