FastQCFastQC Report
Thu 26 May 2016
SRR1546236_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546236_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2977544
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT439221.4751083443267339No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC59800.20083666269919098No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40430.13578304804227914No Hit
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC37890.1272525275864941No Hit
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTG33530.11260958696160325No Hit
ATGTTTGGGTCTTACAAATATTTAAAGGCCTTAAACGTTGATTTACCTTT32950.11066167284177833No Hit
GAGTACGGGTAGCTTTGGGTTATGAAACTCCATGATTTTCATTTAATTTT32410.1088480976267689No Hit
AATAAAGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATT31260.10498585411332292No Hit
TATTTAGGGAGGACTTATTCTTCTTACATGACAAAAAATTGCTCCCCTAT30530.10253416910043982No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGC30390.10206398293358553No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG650.044.00000410
TGATGCG302.5290792E-644.0000041
CTCGTTA700.044.034
GGTCGTA254.4445525E-544.029
ATTACGA351.4471152E-744.030
TAGCGAT351.4471152E-744.017
CGATTCG254.4445525E-544.010
GCGCAAC207.858503E-444.013
GTCGTAA207.858503E-444.011
CATACGT351.4471152E-744.010
CGTAAAT207.858503E-444.016
TACAGCG254.4445525E-544.01
TACGTTG1600.044.01
AATCGTC254.4445525E-544.019
ATATCGT2350.043.99999639
TACGTAG3600.043.388891
TATATCG2400.043.08333638
CGTTTTT314900.043.0149271
CGTAAGG3550.042.7605632
ACGTAGG7100.042.7605632
Produced by FastQC (version 0.11.5)