##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546236_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2977544
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.812627789883205	31.0	31.0	34.0	30.0	34.0
2	32.19928504834857	33.0	31.0	34.0	30.0	34.0
3	32.21210097986797	33.0	31.0	34.0	30.0	34.0
4	35.90959126044821	37.0	35.0	37.0	35.0	37.0
5	35.80584837705169	37.0	35.0	37.0	35.0	37.0
6	35.90155779394024	37.0	35.0	37.0	35.0	37.0
7	36.16214168455613	37.0	35.0	37.0	35.0	37.0
8	36.052932215275405	37.0	35.0	37.0	35.0	37.0
9	37.78396389776272	39.0	38.0	39.0	35.0	39.0
10	37.451772669018496	39.0	37.0	39.0	35.0	39.0
11	37.277792032628234	39.0	37.0	39.0	34.0	39.0
12	36.723153377414405	39.0	35.0	39.0	33.0	39.0
13	36.46882128358137	39.0	35.0	39.0	33.0	39.0
14	37.53182455070353	40.0	35.0	41.0	33.0	41.0
15	37.7538316814126	40.0	35.0	41.0	33.0	41.0
16	37.89373826213819	40.0	35.0	41.0	33.0	41.0
17	37.81788984478483	40.0	35.0	41.0	33.0	41.0
18	37.631168170814604	39.0	36.0	41.0	33.0	41.0
19	37.409758512384705	38.0	36.0	41.0	33.0	41.0
20	37.12735361761237	38.0	35.0	41.0	33.0	41.0
21	37.02305792962253	38.0	35.0	41.0	33.0	41.0
22	36.939795348112405	38.0	35.0	41.0	33.0	41.0
23	36.874984551025946	38.0	35.0	41.0	33.0	41.0
24	36.84099244209322	38.0	35.0	41.0	33.0	41.0
25	36.82128761153488	38.0	35.0	41.0	33.0	41.0
26	36.79370514759815	38.0	35.0	41.0	33.0	41.0
27	36.71307930294229	38.0	35.0	41.0	32.0	41.0
28	36.628752085611495	38.0	35.0	41.0	32.0	41.0
29	36.50889457888783	38.0	35.0	41.0	32.0	41.0
30	36.28467757319455	38.0	35.0	41.0	31.0	41.0
31	35.965707979462266	38.0	35.0	41.0	30.0	41.0
32	35.58517489582018	38.0	35.0	41.0	29.0	41.0
33	35.157967438936254	38.0	35.0	41.0	25.0	41.0
34	34.734372691050076	38.0	34.0	41.0	23.0	41.0
35	34.4235715744251	38.0	34.0	41.0	20.0	41.0
36	34.270405743794214	38.0	34.0	40.0	18.0	41.0
37	34.14135173149415	37.0	34.0	40.0	18.0	41.0
38	34.07833032861983	37.0	34.0	40.0	18.0	41.0
39	34.0138775447147	37.0	34.0	40.0	18.0	41.0
40	33.9179810609012	37.0	34.0	40.0	18.0	41.0
41	33.8421984696112	37.0	34.0	40.0	17.0	41.0
42	33.787242438734744	37.0	34.0	40.0	16.0	41.0
43	33.705024006362294	37.0	33.0	40.0	15.0	41.0
44	33.56673486605068	37.0	33.0	40.0	15.0	41.0
45	33.49995734739772	36.0	33.0	40.0	15.0	41.0
46	33.43963313388484	36.0	33.0	40.0	15.0	41.0
47	33.38131057005371	36.0	33.0	40.0	15.0	41.0
48	33.330773617451165	36.0	33.0	40.0	15.0	41.0
49	33.25621653282034	36.0	33.0	40.0	15.0	41.0
50	33.06411626494856	36.0	33.0	40.0	13.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	1.0
12	2.0
13	13.0
14	19.0
15	49.0
16	177.0
17	440.0
18	1077.0
19	2283.0
20	4127.0
21	6827.0
22	10972.0
23	17087.0
24	26568.0
25	45800.0
26	71609.0
27	88477.0
28	85455.0
29	74173.0
30	71821.0
31	76299.0
32	90342.0
33	116464.0
34	214033.0
35	394723.0
36	166139.0
37	209583.0
38	361114.0
39	841864.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.048911451854277	22.684064450432974	26.736095251657073	19.530928846055676
2	38.23658021510346	23.14666046916519	28.156124645009445	10.46063467072191
3	27.548442609076474	23.137424669459126	38.281684502395265	11.03244821906914
4	24.306273895532694	24.480712963435636	38.6959185153939	12.517094625637773
5	19.997084845765503	29.94803771161736	38.1443901416738	11.910487300943327
6	17.23645393653293	39.39679816654263	35.527535445320034	7.839212451604409
7	77.73292351011438	3.706141706050355	16.734866050678008	1.8260687331572596
8	77.5556297404841	3.430982044261982	16.46417315747475	2.549215057779163
9	73.5018189487712	4.608529714422356	18.67189871921288	3.2177526175935602
10	39.61802075804757	25.872531186776754	24.280648749439134	10.22879930573654
11	33.12464903961117	21.45577025897854	32.729994921989395	12.689585779420891
12	31.963826563100326	19.841218131453306	36.57971133256133	11.615243972885034
13	18.96079453401864	31.86062069947581	35.47890476177682	13.699680004728728
14	12.948524018452792	34.648119389671486	38.079269357564485	14.32408723431123
15	10.648507629106405	23.926363472714428	52.704443662293485	12.720685235885684
16	12.853512828022021	20.292361758549998	49.84124499923427	17.012880414193713
17	13.840198499165757	21.058059931272215	37.37378859892583	27.727952970636206
18	15.463684163861222	24.52914213862163	43.64882601231081	16.358347685206333
19	19.54466499907306	24.73112068201175	37.35874264158649	18.3654716773287
20	20.60701705835413	22.263482924181808	38.93567987576338	18.193820141700677
21	17.549430000026867	26.102687315451927	40.58915670095891	15.758725983562291
22	18.328024707611373	22.137070014750414	36.32749003877021	23.207415238868006
23	13.962446902547871	26.639337655463695	36.79206755634845	22.606147885639977
24	13.934705918703468	23.777146534190592	46.87181784719218	15.416329699913756
25	14.435957957296349	26.504427810302722	41.78450427600734	17.275109956393592
26	13.467878224469562	27.49628552928185	38.50905309879551	20.526783147453067
27	13.658740223486202	32.85002673344206	35.90707643615006	17.584156606921677
28	12.500806033428894	28.510611430091377	40.68316034960356	18.305422186876164
29	13.416056991936978	24.52343273516697	40.122161083093985	21.938349189802064
30	14.791351529985786	28.489520222035342	39.041471763305594	17.677656484673275
31	19.465035613243668	28.81179925468776	34.970331252871496	16.752833879197084
32	20.120575884017164	25.128461577729833	36.8080874707477	17.942875067505298
33	21.572141335274978	27.174073666081846	33.24730045970774	18.006484538935442
34	17.195043969123546	26.142250122920096	34.88223851603872	21.780467391917636
35	16.291614834239226	26.389735970316476	35.96319651363674	21.355452681807556
36	23.901242097513926	26.79735379225294	32.94879941320767	16.35260469702547
37	16.676798059071505	30.050773389075026	37.513937661374605	15.758490890478866
38	16.767577574000587	29.763825488389088	34.64684317007574	18.821753767534585
39	16.426491094674	28.503222790326525	35.88514560993893	19.185140505060545
40	19.319177147340223	25.602509988097573	34.39613318896379	20.682179675598412
41	14.550784136187408	26.531429930170635	35.280889216078755	23.6368967175632
42	16.81221167512554	27.466764554948643	33.00633004919491	22.71469372073091
43	17.46536743033856	24.96429943604528	35.18131050288426	22.389022630731905
44	16.627898697718656	26.858410824491596	34.10700228107461	22.406688196715145
45	16.135983213010455	32.52203829733499	31.7860626073032	19.55591588235136
46	19.738784716531477	29.76775490135494	32.52136660281091	17.97209377930267
47	16.974627411047493	28.729852522750292	34.405268234491245	19.890251831710966
48	16.75894629936619	28.46013358660695	34.974126326932534	19.80679378709433
49	18.940643698296313	26.406125316703964	35.472523663798086	19.180707321201634
50	18.134878947212872	27.593815574177917	33.77894667551512	20.492358803094092
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5865.0
1	9981.0
2	14097.0
3	46426.5
4	78756.0
5	56340.5
6	33925.0
7	36616.0
8	39307.0
9	41446.0
10	43585.0
11	43302.5
12	43020.0
13	40504.0
14	37988.0
15	35855.0
16	33722.0
17	30786.0
18	27850.0
19	25693.5
20	23537.0
21	25719.0
22	27901.0
23	31506.5
24	35112.0
25	34373.5
26	33635.0
27	43730.0
28	53825.0
29	64188.5
30	74552.0
31	93358.5
32	112165.0
33	128085.0
34	144005.0
35	161383.0
36	178761.0
37	173260.5
38	167760.0
39	182139.0
40	196518.0
41	211600.5
42	226683.0
43	237755.5
44	248828.0
45	231622.5
46	214417.0
47	210148.5
48	205880.0
49	195470.0
50	185060.0
51	153216.5
52	121373.0
53	104619.5
54	87866.0
55	76288.0
56	64710.0
57	58453.0
58	52196.0
59	48987.0
60	45778.0
61	42293.0
62	38808.0
63	33567.5
64	28327.0
65	23239.5
66	18152.0
67	15397.5
68	12643.0
69	10412.5
70	8182.0
71	6955.5
72	5729.0
73	4380.0
74	3031.0
75	2580.5
76	2130.0
77	1510.0
78	890.0
79	683.0
80	476.0
81	379.5
82	283.0
83	219.5
84	156.0
85	96.0
86	36.0
87	25.0
88	14.0
89	10.5
90	7.0
91	4.0
92	1.0
93	0.5
94	0.0
95	1.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2977544.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.134553818173064
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.12292880503898	15.52835760640187
2	9.094343856206093	3.662211116275063
3	3.7279307847399736	2.251806690473135
4	2.106547516068604	1.6965757733128837
5	1.3434566267382506	1.3524949876721277
6	0.9133921946886867	1.1034446580635144
7	0.6552196278029085	0.923478840110469
8	0.49914315282275806	0.8040019738785924
9	0.3855363976832372	0.6986343013215943
>10	2.8260083084369283	11.512853558820188
>50	0.5816534921930666	8.425131390250986
>100	0.6228160101115927	23.961960305452862
>500	0.06792807892759746	9.596310009165354
>1k	0.05275803648719107	16.789164513524227
>5k	1.685560271156264E-4	0.20294926388035373
>10k+	1.685560271156264E-4	1.4906250113968056
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	43922	1.4751083443267339	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	5980	0.20083666269919098	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	4043	0.13578304804227914	No Hit
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	3789	0.1272525275864941	No Hit
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTG	3353	0.11260958696160325	No Hit
ATGTTTGGGTCTTACAAATATTTAAAGGCCTTAAACGTTGATTTACCTTT	3295	0.11066167284177833	No Hit
GAGTACGGGTAGCTTTGGGTTATGAAACTCCATGATTTTCATTTAATTTT	3241	0.1088480976267689	No Hit
AATAAAGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATT	3126	0.10498585411332292	No Hit
TATTTAGGGAGGACTTATTCTTCTTACATGACAAAAAATTGCTCCCCTAT	3053	0.10253416910043982	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGC	3039	0.10206398293358553	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14683242296335502	0.0
2	0.0	0.0	0.0	0.5180444016948196	0.0
3	0.0	0.0	0.0	0.712970152582128	0.0
4	0.0	0.0	0.0	1.045257433643298	0.0
5	0.0	0.0	0.0	1.7503687602937186	0.0
6	0.0	0.0	0.0	2.316674413543511	0.0
7	0.0	0.0	0.0	2.687214697750898	0.0
8	0.0	0.0	0.0	3.5881921476223355	0.0
9	0.0	0.0	0.0	3.918430760385069	0.0
10	0.0	0.0	0.0	4.602618802610474	0.0
11	3.3584726203878094E-5	0.0	0.0	5.295807551458518	0.0
12	3.3584726203878094E-5	0.0	0.0	5.904698637534827	0.0
13	3.3584726203878094E-5	0.0	0.0	6.120144656132705	0.0
14	3.3584726203878094E-5	0.0	0.0	6.199001593259411	0.0
15	3.3584726203878094E-5	0.0	0.0	6.331123906145468	0.0
16	3.3584726203878094E-5	0.0	0.0	6.64732410335498	0.0
17	3.3584726203878094E-5	0.0	0.0	7.020987767099328	0.0
18	3.3584726203878094E-5	0.0	0.0	7.604589554344117	0.0
19	3.3584726203878094E-5	0.0	0.0	7.8583893302668235	0.0
20	3.3584726203878094E-5	0.0	0.0	8.134690872746129	0.0
21	3.3584726203878094E-5	0.0	0.0	8.508354536490478	0.0
22	3.3584726203878094E-5	0.0	0.0	8.885141579771785	0.0
23	3.3584726203878094E-5	0.0	0.0	9.339677264215071	0.0
24	3.3584726203878094E-5	0.0	0.0	9.68052865045823	0.0
25	3.3584726203878094E-5	0.0	0.0	9.94621741945711	0.0
26	3.3584726203878094E-5	0.0	0.0	10.244785635409587	0.0
27	3.3584726203878094E-5	0.0	0.0	10.533379187679511	0.0
28	3.3584726203878094E-5	0.0	0.0	10.82633875435594	0.0
29	3.3584726203878094E-5	0.0	0.0	11.15395775847477	0.0
30	3.3584726203878094E-5	0.0	0.0	11.585387151289789	0.0
31	3.3584726203878094E-5	0.0	0.0	11.994885717893673	0.0
32	3.3584726203878094E-5	0.0	0.0	12.338121619697308	0.0
33	3.3584726203878094E-5	0.0	0.0	12.677864709975738	0.0
34	3.3584726203878094E-5	0.0	0.0	13.024794931661798	0.0
35	3.3584726203878094E-5	0.0	0.0	13.509926301676819	0.0
36	3.3584726203878094E-5	0.0	0.0	13.843489802333735	0.0
37	3.3584726203878094E-5	0.0	0.0	14.181520071575768	0.0
38	3.3584726203878094E-5	0.0	0.0	14.534663467609546	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCTACG	65	0.0	44.000004	10
TGATGCG	30	2.5290792E-6	44.000004	1
CTCGTTA	70	0.0	44.0	34
GGTCGTA	25	4.4445525E-5	44.0	29
ATTACGA	35	1.4471152E-7	44.0	30
TAGCGAT	35	1.4471152E-7	44.0	17
CGATTCG	25	4.4445525E-5	44.0	10
GCGCAAC	20	7.858503E-4	44.0	13
GTCGTAA	20	7.858503E-4	44.0	11
CATACGT	35	1.4471152E-7	44.0	10
CGTAAAT	20	7.858503E-4	44.0	16
TACAGCG	25	4.4445525E-5	44.0	1
TACGTTG	160	0.0	44.0	1
AATCGTC	25	4.4445525E-5	44.0	19
ATATCGT	235	0.0	43.999996	39
TACGTAG	360	0.0	43.38889	1
TATATCG	240	0.0	43.083336	38
CGTTTTT	31490	0.0	43.014927	1
CGTAAGG	355	0.0	42.760563	2
ACGTAGG	710	0.0	42.760563	2
>>END_MODULE