FastQCFastQC Report
Thu 26 May 2016
SRR1546235_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546235_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2087475
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293431.405669528976395No Hit
GGAAATGGGAAAATATAGATATATATTAGAACAAGAACAATTTATGTTAA27460.13154648558665372No Hit
TAAGTGAGGGATTTGAGGCTATTTTCACTTATAGAATAGACCATGTTAGA22980.11008515072036791No Hit
AACTAGGGGTCAATGATATAGAATATTATACATTTCATTGTTTTGTTATG22050.10563000754500054No Hit
TTTTAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT21120.10117486436963317No Hit
ATGTACGGCTGGATTTCGATTTAATTTTTTCTTGTTTTTCTAACTTTGTA21010.10064791195104134No Hit
GTGTAAGGGATGCAAATAAAAACTTTGATTTGTTGATTTGTTTTTTTTTT20930.10026467382842907No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACTCG302.5287136E-644.032
TCGTTAG254.4440938E-544.01
GGTACGA254.4440938E-544.011
CGAACTA502.7284841E-1144.043
GTCGAAC900.044.021
CTATCGG1950.044.01
CGCATCG207.8579603E-444.021
GGCGTAT207.8579603E-444.025
GTAATCG207.8579603E-444.01
CATTACG800.044.021
ATCCGAT1100.044.038
TACGACG254.4440938E-544.01
ACGTCGG207.8579603E-444.03
TCATACG800.044.01
CGTTTGG302.5287136E-644.02
CGATACG207.8579603E-444.010
CCGTTGC408.3182385E-944.028
TATCGAA2100.042.9523844
AACGTAC1700.042.70588314
CGTTTTT201150.042.621921
Produced by FastQC (version 0.11.5)