##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546235_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	2087475
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.847515299584426	31.0	31.0	34.0	30.0	34.0
2	32.196404747362244	33.0	31.0	34.0	30.0	34.0
3	32.10465275033234	33.0	31.0	34.0	30.0	34.0
4	35.85548138301058	37.0	35.0	37.0	35.0	37.0
5	35.84830285392639	37.0	35.0	37.0	35.0	37.0
6	35.91845028084169	37.0	35.0	37.0	35.0	37.0
7	36.16902861112109	37.0	35.0	37.0	35.0	37.0
8	36.06706858764775	37.0	35.0	37.0	35.0	37.0
9	37.80645947855663	39.0	38.0	39.0	35.0	39.0
10	37.43318410998934	39.0	37.0	39.0	34.0	39.0
11	37.2747549072444	39.0	37.0	39.0	34.0	39.0
12	36.773201116181035	39.0	35.0	39.0	33.0	39.0
13	36.577014335501026	39.0	35.0	39.0	33.0	39.0
14	37.635872046371816	40.0	35.0	41.0	33.0	41.0
15	37.84811889962754	40.0	35.0	41.0	33.0	41.0
16	37.949905507850396	40.0	35.0	41.0	33.0	41.0
17	37.8588083689625	40.0	35.0	41.0	33.0	41.0
18	37.683118600222755	39.0	36.0	41.0	33.0	41.0
19	37.47377573384112	39.0	36.0	41.0	33.0	41.0
20	37.21156947987401	38.0	35.0	41.0	33.0	41.0
21	37.11743853219799	38.0	35.0	41.0	33.0	41.0
22	37.05557144396939	38.0	35.0	41.0	33.0	41.0
23	37.00428987173499	38.0	35.0	41.0	33.0	41.0
24	36.93583587827399	38.0	35.0	41.0	33.0	41.0
25	36.872256194684965	38.0	35.0	41.0	33.0	41.0
26	36.806746428100936	38.0	35.0	41.0	33.0	41.0
27	36.743277404519816	38.0	35.0	41.0	32.0	41.0
28	36.64890166349298	38.0	35.0	41.0	32.0	41.0
29	36.54777182960275	38.0	35.0	41.0	32.0	41.0
30	36.33848022132002	38.0	35.0	41.0	31.0	41.0
31	35.99828117702008	38.0	35.0	41.0	30.0	41.0
32	35.656853375489526	38.0	35.0	41.0	29.0	41.0
33	35.26302398831124	38.0	35.0	41.0	25.0	41.0
34	34.809671972119425	38.0	34.0	41.0	23.0	41.0
35	34.5724705685098	38.0	34.0	41.0	21.0	41.0
36	34.42544078372196	38.0	34.0	41.0	19.0	41.0
37	34.38853591060971	38.0	34.0	41.0	19.0	41.0
38	34.35436160912107	38.0	34.0	41.0	18.0	41.0
39	34.26407980933904	38.0	34.0	40.0	18.0	41.0
40	34.1152315596594	38.0	34.0	40.0	18.0	41.0
41	33.98302398831124	37.0	34.0	40.0	18.0	41.0
42	33.90522760751626	37.0	34.0	40.0	18.0	41.0
43	33.80350950310782	37.0	33.0	40.0	17.0	41.0
44	33.701961939663946	37.0	33.0	40.0	16.0	41.0
45	33.66422591887328	37.0	33.0	40.0	17.0	41.0
46	33.595610007305474	37.0	33.0	40.0	17.0	41.0
47	33.545918873279916	37.0	33.0	40.0	17.0	41.0
48	33.46347573024827	36.0	33.0	40.0	16.0	41.0
49	33.40483454891675	36.0	33.0	40.0	15.0	41.0
50	33.22630929711733	36.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	3.0
12	5.0
13	7.0
14	25.0
15	52.0
16	127.0
17	360.0
18	799.0
19	1645.0
20	2982.0
21	4951.0
22	7883.0
23	12274.0
24	19318.0
25	31656.0
26	49055.0
27	59802.0
28	57605.0
29	50819.0
30	49139.0
31	52172.0
32	61974.0
33	79074.0
34	142854.0
35	264189.0
36	118863.0
37	151704.0
38	257499.0
39	610637.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.79684786644152	22.590641804093462	26.649420951149118	17.963089378315907
2	38.715050479646465	23.466532533323754	28.00344914310351	9.814967843926274
3	28.46980203355729	23.323728427885364	37.38760943244829	10.818860106109057
4	23.53877291943616	25.69616402591648	38.022251763494175	12.742811291153188
5	19.377573384112385	29.788428603935373	37.5896717325956	13.244326279356638
6	17.904549755086887	39.105234793231055	35.345093953220996	7.645121498461059
7	78.62920514018133	3.540784919579875	16.177535060300123	1.652474879938682
8	78.64716942717877	3.0162277392543624	16.01753314410951	2.319069689457359
9	74.50005389286099	4.463766033126145	17.821578701541334	3.214601372471527
10	39.29910537850753	26.138756152768295	24.62261823494892	9.939520233775255
11	32.51368279859639	22.379333884238132	32.45064012742668	12.6563431897388
12	31.38509443226865	20.751817387034574	35.58763578006922	12.275452400627552
13	18.794620294853832	32.889495682583025	35.72119426579959	12.594689756763556
14	13.569455921627805	35.550317967879856	38.175882345896355	12.704343764595983
15	13.024635025569168	23.93456209056396	50.36074683529144	12.680056048575434
16	15.528138061533673	20.658738428005126	46.90269344543048	16.910430065030717
17	15.701649121546366	20.98937711828884	37.10813303153331	26.20084072863148
18	17.323656570737374	24.533515371441574	42.62920514018132	15.513622917639733
19	20.59555204253943	24.535000419166696	37.04513826512893	17.82430927316495
20	20.486377082360267	25.617791829842275	37.92380747074816	15.972023617049306
21	17.884381848884416	26.463023509263582	39.5049042503503	16.147690391501694
22	18.328794356818644	23.070312219308015	36.415094791554395	22.18579863231895
23	14.934214781015342	26.2577515898394	35.91669361309716	22.891340016048094
24	14.217224158373154	24.44450831746488	45.25649408974958	16.081773434412387
25	15.333309380950668	26.04826405106648	41.40131019533168	17.21711637265117
26	14.439262745661624	27.64785207008467	38.12198948490401	19.790895699349694
27	14.58973161355226	31.35798033509383	36.75742224457778	17.29486580677613
28	14.121558342016073	27.32736919005018	40.87047749074839	17.680594977185354
29	14.460340842405298	25.507706679121906	40.182277632067446	19.849674846405346
30	17.090743601719783	29.393789147175415	36.70305033593217	16.812416915172637
31	20.199762871411632	28.85356710858813	34.0575096707745	16.88916034922574
32	21.51642534641133	25.693577168588845	35.93906513850465	16.850932346495167
33	21.187654941975353	28.950238925017064	32.284841734631556	17.577264398376027
34	16.880681205762944	27.72866740919053	34.37732188409442	21.013329500952107
35	17.83408184529156	27.139007652786262	34.658810285153116	20.368100216769065
36	24.30338087881292	27.10106707864765	31.73460760009102	16.86094444244841
37	16.126229056635406	31.95405933005186	35.301596426304506	16.61811518700823
38	17.223487706439595	30.263595971209234	33.15570246350256	19.35721385884861
39	16.974382926741637	30.189056156361154	33.40796895771207	19.42859195918514
40	19.883016563072612	26.540006467143318	32.19995449047294	21.37702247931113
41	16.86137558533635	26.931963257044995	33.36437562126493	22.842285536353728
42	18.345177786560317	27.896190373537404	32.28900944921496	21.469622390687313
43	17.32068647528713	26.004095857435416	34.51001808404891	22.165199583228542
44	16.257823446987388	28.243931065042695	33.68677469191248	21.81147079605744
45	17.062527694942453	32.85519587060923	30.44664007952191	19.635636354926405
46	20.651696427502124	28.294470592462183	33.190193894537664	17.86363908549803
47	17.284997425118863	29.6694810716296	34.334015976239236	18.7115055270123
48	16.783530341680738	28.872920633780048	34.93574773350579	19.407801291033426
49	19.563731302171284	25.646343069976886	35.68253512018108	19.10739050767075
50	17.54243763398364	29.116707984526762	33.05222817039725	20.288626211092346
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2826.0
1	6013.0
2	9200.0
3	30936.0
4	52672.0
5	38741.5
6	24811.0
7	26341.5
8	27872.0
9	28650.5
10	29429.0
11	29123.0
12	28817.0
13	26578.0
14	24339.0
15	23206.5
16	22074.0
17	20229.5
18	18385.0
19	19495.0
20	20605.0
21	20079.0
22	19553.0
23	23591.5
24	27630.0
25	32841.0
26	38052.0
27	37756.5
28	37461.0
29	47783.5
30	58106.0
31	68959.5
32	79813.0
33	93062.0
34	106311.0
35	111860.0
36	117409.0
37	114028.0
38	110647.0
39	114429.5
40	118212.0
41	140588.5
42	162965.0
43	156579.5
44	150194.0
45	149266.0
46	148338.0
47	143569.0
48	138800.0
49	134834.5
50	130869.0
51	111411.0
52	91953.0
53	77863.0
54	63773.0
55	55874.0
56	47975.0
57	44136.5
58	40298.0
59	38082.5
60	35867.0
61	33078.0
62	30289.0
63	26560.0
64	22831.0
65	20148.5
66	17466.0
67	14050.0
68	10634.0
69	9255.5
70	7877.0
71	6840.0
72	5803.0
73	4387.0
74	2971.0
75	2391.0
76	1811.0
77	1513.0
78	1215.0
79	1007.0
80	799.0
81	538.0
82	277.0
83	211.0
84	145.0
85	93.5
86	42.0
87	36.5
88	31.0
89	20.0
90	9.0
91	6.0
92	3.0
93	4.0
94	5.0
95	3.0
96	1.0
97	5.5
98	10.0
99	5.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2087475.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.859740207439092
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.69919807876241	17.761834824068593
2	9.062423300432894	4.143292845954774
3	3.6154766461507957	2.4794657057121117
4	1.8422639132917216	1.6845469780555542
5	1.176762775083349	1.345024566209522
6	0.7877264000800432	1.0804332516222612
7	0.5754105129172529	0.9207614386542374
8	0.46692945605619224	0.8539108848516324
9	0.37012913773772677	0.7614950338699088
>10	3.055605047198139	15.14185434161639
>50	0.7702494089556808	12.388955715845917
>100	0.48821379072604726	22.16502084213552
>500	0.06720864945584558	10.759775418255101
>1k	0.022191535197684863	7.095962385580074
>5k	0.0	0.0
>10k+	2.1134795426366534E-4	1.4176657675684168
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	29343	1.405669528976395	No Hit
GGAAATGGGAAAATATAGATATATATTAGAACAAGAACAATTTATGTTAA	2746	0.13154648558665372	No Hit
TAAGTGAGGGATTTGAGGCTATTTTCACTTATAGAATAGACCATGTTAGA	2298	0.11008515072036791	No Hit
AACTAGGGGTCAATGATATAGAATATTATACATTTCATTGTTTTGTTATG	2205	0.10563000754500054	No Hit
TTTTAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	2112	0.10117486436963317	No Hit
ATGTACGGCTGGATTTCGATTTAATTTTTTCTTGTTTTTCTAACTTTGTA	2101	0.10064791195104134	No Hit
GTGTAAGGGATGCAAATAAAAACTTTGATTTGTTGATTTGTTTTTTTTTT	2093	0.10026467382842907	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08967772069126577	0.0
2	0.0	0.0	0.0	0.3162193559204302	0.0
3	0.0	0.0	0.0	0.4595504137774105	0.0
4	0.0	0.0	0.0	0.7304518616989425	0.0
5	0.0	0.0	0.0	1.296398759266578	0.0
6	0.0	0.0	0.0	1.7781290793901723	0.0
7	0.0	0.0	0.0	2.074515862465419	0.0
8	0.0	0.0	0.0	2.843195726894933	0.0
9	0.0	0.0	0.0	3.1624330830309346	0.0
10	0.0	0.0	0.0	3.784524365561264	0.0
11	0.0	0.0	0.0	4.439095078982982	0.0
12	0.0	0.0	0.0	4.974574545802944	0.0
13	0.0	0.0	0.0	5.1779781793793935	0.0
14	0.0	0.0	0.0	5.2578354231787205	0.0
15	0.0	0.0	0.0	5.394364004359334	0.0
16	0.0	0.0	0.0	5.6942478353034165	0.0
17	0.0	0.0	0.0	6.03432376435646	0.0
18	0.0	0.0	0.0	6.571623612258829	0.0
19	0.0	0.0	0.0	6.806644390950789	0.0
20	0.0	0.0	0.0	7.0835339345381385	0.0
21	0.0	0.0	0.0	7.4141726248218545	0.0
22	0.0	0.0	0.0	7.750272458352795	0.0
23	0.0	0.0	0.0	8.150325153594654	0.0
24	0.0	0.0	0.0	8.44824488916035	0.0
25	0.0	0.0	0.0	8.676367381645289	0.0
26	0.0	0.0	0.0	8.928202732966861	0.0
27	0.0	0.0	0.0	9.21697265835519	0.0
28	0.0	0.0	0.0	9.480784201008396	0.0
29	0.0	0.0	0.0	9.803326985951928	0.0
30	0.0	0.0	0.0	10.188672918238542	0.0
31	0.0	0.0	0.0	10.534689038192074	0.0
32	0.0	0.0	0.0	10.861016299596402	0.0
33	0.0	0.0	0.0	11.188876513491179	0.0
34	0.0	0.0	0.0	11.537910633660283	0.0
35	0.0	0.0	0.0	11.97389190289704	0.0
36	0.0	0.0	0.0	12.300650307189308	0.0
37	0.0	0.0	0.0	12.655145570605637	0.0
38	4.7904765326530856E-5	0.0	0.0	13.037281883615373	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAACTCG	30	2.5287136E-6	44.0	32
TCGTTAG	25	4.4440938E-5	44.0	1
GGTACGA	25	4.4440938E-5	44.0	11
CGAACTA	50	2.7284841E-11	44.0	43
GTCGAAC	90	0.0	44.0	21
CTATCGG	195	0.0	44.0	1
CGCATCG	20	7.8579603E-4	44.0	21
GGCGTAT	20	7.8579603E-4	44.0	25
GTAATCG	20	7.8579603E-4	44.0	1
CATTACG	80	0.0	44.0	21
ATCCGAT	110	0.0	44.0	38
TACGACG	25	4.4440938E-5	44.0	1
ACGTCGG	20	7.8579603E-4	44.0	3
TCATACG	80	0.0	44.0	1
CGTTTGG	30	2.5287136E-6	44.0	2
CGATACG	20	7.8579603E-4	44.0	10
CCGTTGC	40	8.3182385E-9	44.0	28
TATCGAA	210	0.0	42.95238	44
AACGTAC	170	0.0	42.705883	14
CGTTTTT	20115	0.0	42.62192	1
>>END_MODULE