FastQCFastQC Report
Thu 26 May 2016
SRR1546234_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546234_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences2149866
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300601.3982266801744854No Hit
GGAAATGGGAAAATATAGATATATATTAGAACAAGAACAATTTATGTTAA28230.13131050958524856No Hit
TAAGTGAGGGATTTGAGGCTATTTTCACTTATAGAATAGACCATGTTAGA23440.10903005117528254No Hit
AACTAGGGGTCAATGATATAGAATATTATACATTTCATTGTTTTGTTATG22390.10414602584533175No Hit
ATGTACGGCTGGATTTCGATTTAATTTTTTCTTGTTTTTCTAACTTTGTA21580.10037834916222686No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGC207.8580115E-444.00000423
CTTAACG207.8580115E-444.0000041
AATCCCG207.8580115E-444.00000443
CGAACTA650.044.00000443
CTATCGG1600.044.0000041
CGACCCA207.8580115E-444.00000419
GTTCGCC207.8580115E-444.00000413
CCGGTTA207.8580115E-444.00000430
ACGTACT1300.044.00000415
ACGTACC207.8580115E-444.00000432
TACGTAA408.3182385E-944.00000427
GGTACCG302.52875E-644.01
CTATACG454.802132E-1044.01
GGCGTAA254.4441367E-544.022
GAATCGT254.4441367E-544.05
CGCGATA254.4441367E-544.015
GTGTCGT302.52875E-644.039
TGTTCGA351.4468787E-744.027
CTACCGA351.4468787E-744.028
ACCACGA302.52875E-644.021
Produced by FastQC (version 0.11.5)