FastQCFastQC Report
Thu 26 May 2016
SRR1546233_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546233_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1814717
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT305001.6807028313505632No Hit
TGAAAGGGGACACTTGCATCTGTTTTTCTTTTCAGCCTGGTTAAAATAGC42490.23414119116093585No Hit
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA25770.1420056129964066No Hit
TTTACAGGGTGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA24330.13407049143199737No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23140.12751299513918699No Hit
GATATAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA21100.1162715729229406No Hit
AGAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATTG21020.11583073283602899No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA19440.10712414111952442No Hit
AGGAGAGGGCTCTTTCTAGGATTACCAGGGGTTTTAGGTTGTGTTTGGGA19390.10684861606520465No Hit
GACTTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT19210.1058567258696535No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGC19190.1057465158479256No Hit
AATTGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATTA19160.10558120081533373No Hit
AGAGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATT18980.10458931061978259No Hit
AATGATGGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAAT18720.10315658033731982No Hit
ATATGAGGGGTTTGTGTGGATTTTGCTTATTAGATTTGTTTAGGGGGAAA18680.10293616029386402No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACG302.5285299E-644.0000041
CAAGTCG207.857687E-444.00000418
CGGTTAC302.5285299E-644.00000435
ATAGCGG2400.044.0000042
CCTACGT207.857687E-444.00000444
TTCGTCA207.857687E-444.00000436
CCGTAGG302.5285299E-644.0000042
CGGATTG207.857687E-444.00000441
CTACGTT207.857687E-444.00000427
TCGCTAA302.5285299E-644.00000420
GGTATAC207.857687E-444.00000434
GTATACG207.857687E-444.0000041
TGTACCG800.044.0000041
TATAACG600.044.0000041
ATACCGG408.3164196E-944.0000042
TGCGTAT207.857687E-444.00000419
CTATGCG700.044.01
GTTTACG551.8189894E-1244.01
GGCGTAT254.4438617E-544.027
GAATCGT1400.044.023
Produced by FastQC (version 0.11.5)