FastQCFastQC Report
Thu 26 May 2016
SRR1546232_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546232_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1860441
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT313401.6845468359383609No Hit
TGAAAGGGGACACTTGCATCTGTTTTTCTTTTCAGCCTGGTTAAAATAGC43200.23220300993151624No Hit
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA26890.14453562354302016No Hit
TTTACAGGGTGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA24230.13023793820927404No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24020.1291091735776625No Hit
AGAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATTG21390.11497274033414659No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGC21200.11395147709602185No Hit
AATTGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATTA20970.11271521107092351No Hit
GATATAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA20170.10841515533145099No Hit
ATATGAGGGGTTTGTGTGGATTTTGCTTATTAGATTTGTTTAGGGGGAAA19700.10588887258451088No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA19680.10578137119102407No Hit
GACTTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT19410.10433010237895209No Hit
AATGATGGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAAT18840.10126631266457792No Hit
AGGAGAGGGCTCTTTCTAGGATTACCAGGGGTTTTAGGTTGTGTTTGGGA18810.10110506057434769No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTCC207.857738E-444.00000433
GTCGACC207.857738E-444.00000413
ACCGAGT207.857738E-444.00000433
GTGTCGC207.857738E-444.00000429
CGTAATC207.857738E-444.00000434
CGTCGTA207.857738E-444.00000431
ATAACCG207.857738E-444.00000410
ACTCCCG207.857738E-444.00000410
GGCGAAT207.857738E-444.00000410
TCACGAT1200.044.038
GTCATCG600.044.043
CTAGACG302.5285644E-644.01
GACCGTA254.4439053E-544.044
GACCGGA254.4439053E-544.01
GCAAACG700.044.01
CGTTACC502.7284841E-1144.034
TACGCTA254.4439053E-544.012
CTACGTT551.8189894E-1244.027
TCACTAC302.5285644E-644.027
ACGTCAT254.4439053E-544.015
Produced by FastQC (version 0.11.5)