##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546232_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1860441
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.854116308982654	31.0	31.0	34.0	30.0	34.0
2	32.235161985787244	33.0	31.0	34.0	30.0	34.0
3	32.22209250387408	33.0	31.0	34.0	30.0	34.0
4	35.94150419174809	37.0	35.0	37.0	35.0	37.0
5	35.87428142037291	37.0	35.0	37.0	35.0	37.0
6	35.954540348229266	37.0	35.0	37.0	35.0	37.0
7	36.18915998948636	37.0	35.0	37.0	35.0	37.0
8	36.085864588019724	37.0	35.0	37.0	35.0	37.0
9	37.841539183451665	39.0	38.0	39.0	35.0	39.0
10	37.49344107122989	39.0	37.0	39.0	35.0	39.0
11	37.32966753581543	39.0	37.0	39.0	34.0	39.0
12	36.6942165862825	39.0	35.0	39.0	33.0	39.0
13	36.42485249465046	39.0	35.0	39.0	33.0	39.0
14	37.54370012271284	40.0	35.0	41.0	33.0	41.0
15	37.769891117213604	40.0	35.0	41.0	33.0	41.0
16	37.859530616665616	40.0	35.0	41.0	33.0	41.0
17	37.7768432323304	40.0	35.0	41.0	33.0	41.0
18	37.57197890177651	39.0	36.0	41.0	33.0	41.0
19	37.27224835401929	38.0	35.0	41.0	33.0	41.0
20	36.93223810913649	38.0	35.0	41.0	33.0	41.0
21	36.84416221745274	38.0	35.0	41.0	33.0	41.0
22	36.76288417638614	37.0	35.0	41.0	33.0	41.0
23	36.7068480000172	37.0	35.0	41.0	33.0	41.0
24	36.658426684855904	37.0	35.0	41.0	33.0	41.0
25	36.63102135461431	37.0	35.0	41.0	33.0	41.0
26	36.59220152641229	37.0	35.0	41.0	33.0	41.0
27	36.493307231995	37.0	35.0	41.0	32.0	41.0
28	36.40942711969904	37.0	35.0	41.0	32.0	41.0
29	36.285305473272196	37.0	35.0	41.0	32.0	41.0
30	36.051717845392574	37.0	35.0	40.0	31.0	41.0
31	35.653434320142374	37.0	35.0	40.0	30.0	41.0
32	35.18736955377784	37.0	35.0	40.0	27.0	41.0
33	34.662584301249005	37.0	34.0	40.0	23.0	41.0
34	34.210515678809486	37.0	34.0	40.0	19.0	41.0
35	33.87928883528153	37.0	34.0	40.0	18.0	41.0
36	33.71276756424955	36.0	33.0	40.0	16.0	41.0
37	33.603201068993855	36.0	33.0	40.0	15.0	41.0
38	33.576547173492735	36.0	33.0	40.0	15.0	41.0
39	33.51253493123404	36.0	33.0	40.0	15.0	41.0
40	33.382666797818366	36.0	33.0	40.0	15.0	41.0
41	33.30183757506957	36.0	33.0	40.0	15.0	41.0
42	33.219698985348096	36.0	33.0	40.0	12.0	41.0
43	33.09116763176043	36.0	33.0	40.0	12.0	41.0
44	32.947760772849016	35.0	33.0	40.0	10.0	41.0
45	32.88355234054721	35.0	33.0	40.0	10.0	41.0
46	32.82598050677232	35.0	33.0	40.0	10.0	41.0
47	32.75444907954619	35.0	32.0	40.0	10.0	41.0
48	32.69312705965951	35.0	32.0	40.0	10.0	41.0
49	32.64595974825324	35.0	32.0	40.0	10.0	41.0
50	32.45775920870374	35.0	32.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	1.0
12	4.0
13	9.0
14	18.0
15	39.0
16	139.0
17	314.0
18	774.0
19	1508.0
20	2858.0
21	4705.0
22	7431.0
23	11489.0
24	18355.0
25	31109.0
26	49946.0
27	61846.0
28	60025.0
29	52076.0
30	48582.0
31	49453.0
32	56341.0
33	72295.0
34	137133.0
35	264906.0
36	101553.0
37	123713.0
38	211235.0
39	492580.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.29808523892991	22.012146582450075	25.66563519079616	22.024132987823855
2	38.29645766783252	24.598146353472107	26.522636299673035	10.58275967902234
3	24.812396630691325	24.288058583959394	40.79871385332832	10.100830932020957
4	22.0384306731576	25.66026012112182	40.18665466951115	12.114654536209425
5	19.888994061085516	28.124783317503756	40.568123364299105	11.41809925711162
6	16.92061183343089	39.08014282635139	36.59428060336232	7.404964736855401
7	76.5064304646049	3.5436221842025626	18.460999300703435	1.4889480504891044
8	76.64139846412759	2.8986138232816843	18.36199051730208	2.097997195288644
9	73.16711467872402	4.026840947925788	20.160327578246235	2.6457167951039566
10	40.33699536830247	23.977809562356452	26.187554456174638	9.497640613166448
11	34.68150830905146	18.820268957736364	34.00747457188914	12.490748161323042
12	31.724252475622716	19.394003894775487	36.80691835967924	12.074825269922561
13	18.16047915521105	32.59076745782317	36.12084446644639	13.127908920519381
14	12.344546266180975	34.92634273271767	39.60899593160976	13.12011506949159
15	11.092423785543321	22.615874408272017	54.500411461583575	11.791290344601093
16	13.705191403543568	18.63746283811204	51.12734023814783	16.530005520196557
17	12.727143725600543	20.136408518195417	39.84684276469934	27.289604991504703
18	16.028834023761032	23.357902776814747	44.429895922525894	16.183367276898327
19	19.798854142646825	23.31678349380604	39.327019776493856	17.557342587053284
20	22.09820144793627	21.258830567591232	39.84786402793746	16.795103956535037
21	17.0065591975236	24.292412390395608	41.97144655487597	16.729581857204824
22	19.226301720936057	21.221258830567592	37.05497782515006	22.497461623346293
23	14.135626983064768	25.087062691050132	37.75943445666915	23.017875869215953
24	13.166018164510456	22.36770744140771	49.61474188109163	14.8515325129902
25	13.36629326057639	24.13492284893743	45.16810799159984	17.330675898886337
26	13.517923976089541	27.749442202144547	40.108447405749494	18.624186416016418
27	13.543670559829632	31.819444959555288	37.16753178413075	17.46935269648433
28	12.882859494066192	27.86548995641356	41.8928630362371	17.35878751328314
29	13.224767676051	23.662722978046602	41.8195470858791	21.292962260023295
30	15.62059748199486	29.813791461271816	38.745114733549734	15.820496323183589
31	20.691008207193885	28.17966277887877	35.44277942702832	15.686549586899021
32	21.108812372980385	25.08926646961661	36.80982089730338	16.992100260099622
33	20.763087891526794	29.64958308272071	32.106204926681364	17.481124099071135
34	17.080896411119728	27.97148633039156	34.63748648841861	20.310130770070106
35	17.202856742030516	27.493212630768728	34.27483053749084	21.029100089709914
36	23.86240681644836	27.91612311274585	32.38087098704017	15.840599083765625
37	17.04697972147464	31.07521281244608	36.06720127109648	15.810606194982801
38	16.37004344668818	28.84773018870257	34.09524945967112	20.68697690493813
39	16.46254839578358	29.924840400743697	34.715048743819345	18.897562459653383
40	19.027800397862656	25.87311288022571	33.92819229419261	21.17089442771902
41	13.71024396903745	27.485257527650703	35.74996465891689	23.05453384439496
42	17.598408119365246	26.97951722199199	33.526835841609596	21.895238817033167
43	17.58599170841752	25.56893768735477	35.02239522779814	21.82267537642957
44	16.568921024638783	28.570591596293564	33.41256186033311	21.447925518734536
45	15.40425092760265	34.49477838856486	30.28545382519521	19.81551685863728
46	21.448516776398712	29.681295993799317	32.738528123170795	16.13165910663117
47	17.69247183866621	30.305610336473986	33.14520589473141	18.856711930128395
48	17.127014508925573	28.39267679007289	34.665705604208895	19.814603096792645
49	20.011061893389794	24.926455609180834	35.908045458039254	19.154437039390125
50	18.566565669107487	28.65331391858167	32.33319411902877	20.44692629328208
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4552.0
1	7433.0
2	10314.0
3	35687.5
4	61061.0
5	44329.0
6	27597.0
7	28136.5
8	28676.0
9	30167.5
10	31659.0
11	31226.0
12	30793.0
13	29868.0
14	28943.0
15	25941.0
16	22939.0
17	20857.0
18	18775.0
19	17256.5
20	15738.0
21	15797.0
22	15856.0
23	17622.5
24	19389.0
25	22003.0
26	24617.0
27	27293.0
28	29969.0
29	32939.0
30	35909.0
31	54129.0
32	72349.0
33	82016.5
34	91684.0
35	100470.0
36	109256.0
37	98266.5
38	87277.0
39	105373.0
40	123469.0
41	131721.5
42	139974.0
43	140444.0
44	140914.0
45	137447.5
46	133981.0
47	127262.0
48	120543.0
49	112083.5
50	103624.0
51	87650.5
52	71677.0
53	62756.5
54	53836.0
55	47015.0
56	40194.0
57	39021.5
58	37849.0
59	37271.0
60	36693.0
61	33046.5
62	29400.0
63	25741.0
64	22082.0
65	17595.0
66	13108.0
67	10952.5
68	8797.0
69	7408.0
70	6019.0
71	5601.5
72	5184.0
73	4061.5
74	2939.0
75	2153.0
76	1367.0
77	1079.0
78	791.0
79	546.0
80	301.0
81	276.5
82	252.0
83	158.5
84	65.0
85	41.0
86	17.0
87	11.5
88	6.0
89	4.5
90	3.0
91	2.0
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1860441.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.54160736448651
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.96493787176273	17.799966250663466
2	8.564865791419669	3.8613168359940837
3	3.3972309652887693	2.297371396380448
4	1.793414187060749	1.6170575378649268
5	1.1292816060857567	1.2727911284160924
6	0.7426774974771077	1.0044686727941028
7	0.5663033713227216	0.8935771771879261
8	0.4242827277742186	0.7651211728815801
9	0.3308673599066078	0.6712453905065099
>10	2.7488771266223186	13.645816588267126
>50	0.7134202391532277	11.556539285433901
>100	0.5261109285654098	22.60844979521387
>500	0.0657938814537223	10.272577392331952
>1k	0.03169632245215819	10.037338271293958
>5k	0.0	0.0
>10k+	2.4012365494059234E-4	1.6963631047700722
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	31340	1.6845468359383609	No Hit
TGAAAGGGGACACTTGCATCTGTTTTTCTTTTCAGCCTGGTTAAAATAGC	4320	0.23220300993151624	No Hit
GGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTA	2689	0.14453562354302016	No Hit
TTTACAGGGTGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA	2423	0.13023793820927404	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2402	0.1291091735776625	No Hit
AGAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATTG	2139	0.11497274033414659	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGC	2120	0.11395147709602185	No Hit
AATTGAGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTATTA	2097	0.11271521107092351	No Hit
GATATAGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA	2017	0.10841515533145099	No Hit
ATATGAGGGGTTTGTGTGGATTTTGCTTATTAGATTTGTTTAGGGGGAAA	1970	0.10588887258451088	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1968	0.10578137119102407	No Hit
GACTTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1941	0.10433010237895209	No Hit
AATGATGGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAAT	1884	0.10126631266457792	No Hit
AGGAGAGGGCTCTTTCTAGGATTACCAGGGGTTTTAGGTTGTGTTTGGGA	1881	0.10110506057434769	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08702237802757518	0.0
2	0.0	0.0	0.0	0.3405644145662238	0.0
3	0.0	0.0	0.0	0.49805395602440494	0.0
4	0.0	0.0	0.0	0.7844914189700184	0.0
5	0.0	0.0	0.0	1.3651064451922958	0.0
6	0.0	0.0	0.0	1.8522490097777893	0.0
7	0.0	0.0	0.0	2.1599717486337915	0.0
8	0.0	0.0	0.0	2.889314952745075	0.0
9	0.0	0.0	0.0	3.1703235953196045	0.0
10	5.375069674340654E-5	0.0	0.0	3.69584415737989	0.0
11	5.375069674340654E-5	0.0	0.0	4.308924604435185	0.0
12	5.375069674340654E-5	0.0	0.0	4.815041164971101	0.0
13	5.375069674340654E-5	0.0	0.0	4.993708480946184	0.0
14	5.375069674340654E-5	0.0	0.0	5.05423176547926	0.0
15	5.375069674340654E-5	0.0	0.0	5.16485069937719	0.0
16	5.375069674340654E-5	0.0	0.0	5.428121612026396	0.0
17	5.375069674340654E-5	0.0	0.0	5.7349843397345035	0.0
18	5.375069674340654E-5	0.0	0.0	6.2126130309964145	0.0
19	5.375069674340654E-5	0.0	0.0	6.431915873709513	0.0
20	5.375069674340654E-5	0.0	0.0	6.68164161077938	0.0
21	5.375069674340654E-5	0.0	0.0	6.995760682547847	0.0
22	5.375069674340654E-5	0.0	0.0	7.306385959027994	0.0
23	5.375069674340654E-5	0.0	0.0	7.669525666226448	0.0
24	5.375069674340654E-5	0.0	0.0	7.950534308800978	0.0
25	5.375069674340654E-5	0.0	0.0	8.167310868767137	0.0
26	5.375069674340654E-5	0.0	0.0	8.393708803450364	0.0
27	5.375069674340654E-5	0.0	0.0	8.613979158704845	0.0
28	5.375069674340654E-5	0.0	0.0	8.8625761311431	0.0
29	5.375069674340654E-5	0.0	0.0	9.12181574153655	0.0
30	5.375069674340654E-5	0.0	0.0	9.471840278729614	0.0
31	5.375069674340654E-5	0.0	0.0	9.822026068012907	0.0
32	5.375069674340654E-5	0.0	0.0	10.107764771900856	0.0
33	5.375069674340654E-5	0.0	0.0	10.372056947788186	0.0
34	5.375069674340654E-5	0.0	0.0	10.647421767204657	0.0
35	5.375069674340654E-5	0.0	0.0	11.016312798954656	0.0
36	5.375069674340654E-5	0.0	0.0	11.299417718702179	0.0
37	5.375069674340654E-5	0.0	0.0	11.60128163161315	0.0
38	5.375069674340654E-5	0.0	0.0	11.867078827009296	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTCC	20	7.857738E-4	44.000004	33
GTCGACC	20	7.857738E-4	44.000004	13
ACCGAGT	20	7.857738E-4	44.000004	33
GTGTCGC	20	7.857738E-4	44.000004	29
CGTAATC	20	7.857738E-4	44.000004	34
CGTCGTA	20	7.857738E-4	44.000004	31
ATAACCG	20	7.857738E-4	44.000004	10
ACTCCCG	20	7.857738E-4	44.000004	10
GGCGAAT	20	7.857738E-4	44.000004	10
TCACGAT	120	0.0	44.0	38
GTCATCG	60	0.0	44.0	43
CTAGACG	30	2.5285644E-6	44.0	1
GACCGTA	25	4.4439053E-5	44.0	44
GACCGGA	25	4.4439053E-5	44.0	1
GCAAACG	70	0.0	44.0	1
CGTTACC	50	2.7284841E-11	44.0	34
TACGCTA	25	4.4439053E-5	44.0	12
CTACGTT	55	1.8189894E-12	44.0	27
TCACTAC	30	2.5285644E-6	44.0	27
ACGTCAT	25	4.4439053E-5	44.0	15
>>END_MODULE