FastQCFastQC Report
Thu 26 May 2016
SRR1546231_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546231_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1612982
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT320851.9891728487980647No Hit
ATAATGGGGTTTCCCATTGGTAGTTGTCTTCTCTTTGGTCAAGATGAACT25340.15710032722001857No Hit
ATGTTATAGGGATTGCCCATATCTGCATTTTTTTCTTTGAATATTTACAC23780.14742879957742863No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC23350.14476292977850963No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA22210.13769527496277081No Hit
TGTATTGGGTAAAATATTTTGGCTATATAGTTTTAGTATTCATCTTAGAG21800.13515339910798757No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21120.13093760500737145No Hit
GTAGAAGGGGGTGAAGTAAAATGGTGAAGCTTATGAATTTGTGGAGTGAG20710.12839572915258818No Hit
TAAGCTAGGGGTTTTTAACTGAACTATTTGGATTTTGTTTCTCTTCTAGG20510.1271557897112305No Hit
AGGAACGGGGGAAGGACTGTAATCATAATAAGCTCACTACTTGGCTATTG20280.12572985935366915No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17940.11122256788978425No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGC17170.10644880104055718No Hit
TAATGGGGTCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCC17140.10626281012435351No Hit
AAAAAAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT16650.10322495849302721No Hit
TGCATGGGCAAGGATTAATTTTGTACTTTTTTTTCTCCTCCTTTAGTAAA16400.10167503419133009No Hit
AAAAAAGGGGGGGTGAAAACAAGAAAGAATAGGATGGAATCTGGAATAAA16300.10105506447065125No Hit
AAAATAGGGATACTAAATAACTCTATAACCATCAACTCAATCTCACTTCT16180.10031110080583663No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAT302.5283534E-644.00000444
CGACGGC207.8574265E-444.00000434
CGTTGAC207.8574265E-444.00000419
ACTAGAC207.8574265E-444.00000427
GATATCG207.8574265E-444.00000425
ACGCTAT207.8574265E-444.00000443
CGTACAA207.8574265E-444.00000426
GCGTGTA207.8574265E-444.00000414
TCGAGTG207.8574265E-444.00000436
TAGGCCG1200.044.00000440
ATAGTAC600.044.00000439
CAATCGT207.8574265E-444.00000415
GCGTACA207.8574265E-444.00000425
TACGTAG302.5283534E-644.0000041
CTTAACG1100.044.01
CTAGACG502.7284841E-1144.01
AAGTACG351.4465695E-744.01
TCCATAC254.4436412E-544.044
GTCGATA254.4436412E-544.09
GTCGAAC254.4436412E-544.021
Produced by FastQC (version 0.11.5)