FastQCFastQC Report
Thu 26 May 2016
SRR1546230_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546230_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1640962
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT330232.012417106550913No Hit
ATAATGGGGTTTCCCATTGGTAGTTGTCTTCTCTTTGGTCAAGATGAACT25630.15618886969960302No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23360.1423555207250381No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC23350.14229458086171404No Hit
ATGTTATAGGGATTGCCCATATCTGCATTTTTTTCTTTGAATATTTACAC22900.1395522870121307No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22310.13595683507601028No Hit
TGTATTGGGTAAAATATTTTGGCTATATAGTTTTAGTATTCATCTTAGAG22260.13565213575938992No Hit
TAAGCTAGGGGTTTTTAACTGAACTATTTGGATTTTGTTTCTCTTCTAGG21640.13187386423329728No Hit
GTAGAAGGGGGTGAAGTAAAATGGTGAAGCTTATGAATTTGTGGAGTGAG20720.12626739680748245No Hit
AGGAACGGGGGAAGGACTGTAATCATAATAAGCTCACTACTTGGCTATTG20080.12236724555474167No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18320.11164182960970453No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGC17690.10780261822028786No Hit
TAATGGGGTCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCC16950.10329306833430633No Hit
AAAAAAGGGGGGGTGAAAACAAGAAAGAATAGGATGGAATCTGGAATAAA16550.10085547380134337No Hit
AAAATAGGGATACTAAATAACTCTATAACCATCAACTCAATCTCACTTCT16470.10036795489475075No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGTA302.5283825E-644.031
CTAGACG408.3164196E-944.01
AATCCGG351.4465877E-744.02
ATTAGCG502.7284841E-1144.01
CGCATCG254.4436747E-544.021
ATAGACG600.044.01
CGATTCG302.5283825E-644.010
CACGACG1150.044.026
CTGCGTA254.4436747E-544.019
ACGATTC207.8574684E-444.017
CCGTAGG207.8574684E-444.02
TACGATG408.3164196E-944.01
CATACGA1900.044.018
ATGCGTC207.8574684E-444.011
CATCGGC207.8574684E-444.028
GCGTGTA302.5283825E-644.014
ACCGTAT207.8574684E-444.035
ATCGGTT1600.044.02
ATCGGCC207.8574684E-444.029
GTAGTAT302.5283825E-644.015
Produced by FastQC (version 0.11.5)