##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546230_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1640962
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.815209614847877	31.0	31.0	34.0	30.0	34.0
2	32.208661748413434	33.0	31.0	34.0	30.0	34.0
3	32.201815764167605	33.0	31.0	34.0	30.0	34.0
4	35.93579010360995	37.0	35.0	37.0	35.0	37.0
5	35.844423575926804	37.0	35.0	37.0	35.0	37.0
6	35.9258544682936	37.0	35.0	37.0	35.0	37.0
7	36.16227980903884	37.0	35.0	37.0	35.0	37.0
8	36.06800157468607	37.0	35.0	37.0	35.0	37.0
9	37.836829859557994	39.0	38.0	39.0	35.0	39.0
10	37.416066307446485	39.0	37.0	39.0	34.0	39.0
11	37.2510137346264	39.0	37.0	39.0	34.0	39.0
12	36.64011598074788	39.0	35.0	39.0	33.0	39.0
13	36.330609727708506	39.0	35.0	39.0	32.0	39.0
14	37.28424911728608	40.0	35.0	41.0	32.0	41.0
15	37.59533554098145	40.0	35.0	41.0	33.0	41.0
16	37.72931061170216	40.0	35.0	41.0	33.0	41.0
17	37.66555898308431	40.0	35.0	41.0	33.0	41.0
18	37.436875442575754	39.0	35.0	41.0	33.0	41.0
19	37.14692479167708	37.0	35.0	41.0	33.0	41.0
20	36.80352013026505	37.0	35.0	41.0	33.0	41.0
21	36.69580953123838	37.0	35.0	41.0	33.0	41.0
22	36.63780331293473	37.0	35.0	41.0	32.0	41.0
23	36.61149130814729	37.0	35.0	41.0	32.0	41.0
24	36.53512147142956	37.0	35.0	40.0	32.0	41.0
25	36.49911698138043	37.0	35.0	40.0	32.0	41.0
26	36.4677414833494	37.0	35.0	40.0	32.0	41.0
27	36.3922814787911	37.0	35.0	41.0	32.0	41.0
28	36.300155031012295	37.0	35.0	40.0	32.0	41.0
29	36.168955771065995	37.0	35.0	40.0	31.0	41.0
30	35.9030117699252	36.0	35.0	40.0	31.0	41.0
31	35.49996831127107	36.0	35.0	40.0	29.0	41.0
32	35.04683228496455	36.0	35.0	40.0	25.0	41.0
33	34.529625305156365	36.0	34.0	40.0	22.0	41.0
34	34.03831532966638	36.0	34.0	40.0	18.0	41.0
35	33.72537633412596	36.0	34.0	40.0	16.0	41.0
36	33.54950876376174	36.0	33.0	40.0	15.0	41.0
37	33.438411736530156	36.0	33.0	40.0	15.0	41.0
38	33.37118714510147	36.0	33.0	40.0	15.0	41.0
39	33.3128634301099	36.0	33.0	40.0	15.0	41.0
40	33.1851749156897	36.0	33.0	40.0	12.0	41.0
41	33.08666623602497	36.0	33.0	40.0	11.0	41.0
42	32.98221104449707	36.0	33.0	40.0	10.0	41.0
43	32.89322299967946	35.0	33.0	40.0	10.0	41.0
44	32.7364923745949	35.0	32.0	40.0	10.0	41.0
45	32.711797713780086	35.0	32.0	40.0	10.0	41.0
46	32.65944549599564	35.0	32.0	40.0	10.0	41.0
47	32.609652143072175	35.0	32.0	40.0	10.0	41.0
48	32.56877368275438	35.0	32.0	40.0	10.0	41.0
49	32.48869687415065	35.0	32.0	40.0	10.0	41.0
50	32.31561486493898	35.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	3.0
11	2.0
12	4.0
13	7.0
14	15.0
15	44.0
16	116.0
17	328.0
18	780.0
19	1549.0
20	2872.0
21	4695.0
22	7435.0
23	11536.0
24	17777.0
25	29923.0
26	47055.0
27	57336.0
28	54293.0
29	46282.0
30	43295.0
31	44977.0
32	51648.0
33	66730.0
34	126554.0
35	217251.0
36	87879.0
37	107762.0
38	184102.0
39	428708.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.596547634862965	23.22271935608503	27.89570995550171	24.285023053550294
2	37.84176598848724	24.59094116743715	27.205992582399833	10.361300261675774
3	23.461847379768695	23.69299228135691	41.30272364625141	11.542436692622985
4	22.126715914201547	24.19611179296047	41.94356724896737	11.733605043870607
5	18.678494687872114	27.412700598795098	42.33175417834173	11.57705053499106
6	18.045268568071656	36.066892469173574	38.05621336752466	7.831625595230116
7	76.45972301613322	3.0955622372730143	19.02122047920671	1.4234942673870572
8	76.93145849812488	2.3598962072247867	18.766187151195457	1.9424581434548758
9	73.86856002759356	3.366318050021878	20.196445743411488	2.5686761789730657
10	40.47528218203712	21.047714694185483	28.204187543648178	10.272815580129217
11	34.993558656446645	19.217385899246906	33.59255119862617	12.196504245680277
12	33.64873775261097	17.9352721147717	36.41522472793398	12.00076540468335
13	19.25827654753736	29.939084512621257	36.614741840457	14.187897099384386
14	12.319115250688316	33.001312644656	39.27068390371014	15.408888200945542
15	12.874582104887256	22.0985007574825	51.839469774437184	13.187447363193053
16	15.011864991389197	18.733645264180403	49.187976321206705	17.066513423223697
17	14.989926640592529	18.86448315073719	39.6107892809218	26.53480092774848
18	16.69313488063709	23.03892472829962	44.151479437061916	16.116460954001372
19	20.205830482363393	24.14163155514875	37.54925464453168	18.103283317956176
20	20.163477277353163	22.350426152464227	40.2428575433191	17.24323902686351
21	17.010875328008815	24.791372377910033	41.79140041024716	16.406351883833995
22	18.02515841317471	20.50864066322072	39.32674857796829	22.13945234563628
23	14.760427115314064	25.17498881753508	38.10246672378763	21.96211734336322
24	14.417518504389498	22.530503448586867	48.21756993763414	14.834408109389493
25	15.448377232379542	23.757040077710514	43.38004170724246	17.414540982667486
26	13.181414316723972	26.58653887171062	39.432662060425535	20.79938475113988
27	12.887318536321986	30.661770351781453	38.75104968914576	17.6998614227508
28	11.805087503549746	27.082467479441934	43.322575416127854	17.789869600880458
29	14.045724398249321	22.858055213953765	41.64374312141293	21.452477266383987
30	16.19720627290577	26.856075887192997	40.484118462219115	16.462599377682118
31	20.219907590791255	27.369920814741594	36.12716199400108	16.283009600466066
32	21.084827070949846	23.730287477711247	37.36442403906977	17.820461412269147
33	21.584412070480607	25.38352502983006	34.7663748459745	18.265688053714833
34	15.60505362098574	25.212893412522654	36.91212837347849	22.269924593013123
35	16.697095971753154	25.491327648050348	36.04513693796687	21.76643944222962
36	24.18953028772147	25.894993302709018	33.86385547014495	16.051620939424556
37	17.411554929364602	28.71285258281423	36.29182150470273	17.583770983118438
38	17.673474461931477	28.277985718133632	34.31212910475684	19.73641071517805
39	15.841622170409797	27.71398728306932	35.71191776531084	20.732472781210046
40	18.054287667843617	25.240438230745138	35.07466961453099	21.630604486880255
41	14.776515239231621	24.94451425444343	35.91643194662643	24.362538559698518
42	17.573045567173402	25.21356375101922	34.07519491615284	23.138195765654537
43	18.148866335722584	24.605932373814873	34.61347672889439	22.631724561568152
44	16.596423317541785	26.15045320976354	34.238696569451335	23.01442690324334
45	16.87455285375286	31.818591777262363	29.741456535861282	21.5653988331235
46	20.245563273250692	29.305248994187554	32.53493987063686	17.914247861924895
47	17.08369846468108	28.462877263458875	34.27519954758245	20.178224724277587
48	18.09335012023435	26.471545349618093	34.913849315218755	20.521255214928804
49	19.18533153113844	25.119167902730226	35.34902087921597	20.34647968691536
50	17.655740961704172	26.520540999730645	34.39324006284119	21.430477975723996
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3349.0
1	6579.0
2	9809.0
3	33953.0
4	58097.0
5	41100.5
6	24104.0
7	24729.5
8	25355.0
9	26711.5
10	28068.0
11	28138.5
12	28209.0
13	26065.5
14	23922.0
15	21795.5
16	19669.0
17	18523.5
18	17378.0
19	14693.0
20	12008.0
21	11896.0
22	11784.0
23	13326.5
24	14869.0
25	18170.0
26	21471.0
27	22353.5
28	23236.0
29	31315.0
30	39394.0
31	45726.0
32	52058.0
33	57029.5
34	62001.0
35	65015.5
36	68030.0
37	74544.0
38	81058.0
39	88434.5
40	95811.0
41	107598.5
42	119386.0
43	120372.0
44	121358.0
45	120462.0
46	119566.0
47	117003.5
48	114441.0
49	111597.5
50	108754.0
51	93752.5
52	78751.0
53	68743.5
54	58736.0
55	53533.0
56	48330.0
57	43804.0
58	39278.0
59	35975.5
60	32673.0
61	29210.5
62	25748.0
63	22355.0
64	18962.0
65	15903.5
66	12845.0
67	10544.0
68	8243.0
69	6846.0
70	5449.0
71	4443.5
72	3438.0
73	3052.0
74	2666.0
75	2041.5
76	1417.0
77	972.0
78	527.0
79	486.5
80	446.0
81	306.0
82	166.0
83	103.0
84	40.0
85	33.0
86	26.0
87	24.5
88	23.0
89	15.5
90	8.0
91	5.5
92	3.0
93	2.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1640962.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.304369499303668
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.11662051167666	20.019961989636542
2	8.594254271954092	4.349443713369908
3	3.317413763732844	2.5183519097871474
4	1.7582781171976314	1.7796847664043534
5	1.1198820774796607	1.4168954942096579
6	0.7376105563175982	1.1198862038188466
7	0.5464711676488184	0.9679675842831137
8	0.42047373444275205	0.8511858192873188
9	0.33641254453154085	0.7661436597924356
>10	2.8814323097728023	16.075823388572516
>50	0.7027437494628885	12.453706383627718
>100	0.39861864311510004	20.327678728395462
>500	0.05234141450073875	9.194188856775698
>1k	0.017204816803483573	6.134180715625164
>5k	0.0	0.0
>10k+	2.423213634293461E-4	2.0249007864141966
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	33023	2.012417106550913	No Hit
ATAATGGGGTTTCCCATTGGTAGTTGTCTTCTCTTTGGTCAAGATGAACT	2563	0.15618886969960302	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2336	0.1423555207250381	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	2335	0.14229458086171404	No Hit
ATGTTATAGGGATTGCCCATATCTGCATTTTTTTCTTTGAATATTTACAC	2290	0.1395522870121307	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2231	0.13595683507601028	No Hit
TGTATTGGGTAAAATATTTTGGCTATATAGTTTTAGTATTCATCTTAGAG	2226	0.13565213575938992	No Hit
TAAGCTAGGGGTTTTTAACTGAACTATTTGGATTTTGTTTCTCTTCTAGG	2164	0.13187386423329728	No Hit
GTAGAAGGGGGTGAAGTAAAATGGTGAAGCTTATGAATTTGTGGAGTGAG	2072	0.12626739680748245	No Hit
AGGAACGGGGGAAGGACTGTAATCATAATAAGCTCACTACTTGGCTATTG	2008	0.12236724555474167	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1832	0.11164182960970453	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGC	1769	0.10780261822028786	No Hit
TAATGGGGTCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCC	1695	0.10329306833430633	No Hit
AAAAAAGGGGGGGTGAAAACAAGAAAGAATAGGATGGAATCTGGAATAAA	1655	0.10085547380134337	No Hit
AAAATAGGGATACTAAATAACTCTATAACCATCAACTCAATCTCACTTCT	1647	0.10036795489475075	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04351106241338922	0.0
2	0.0	0.0	0.0	0.2244415166225665	0.0
3	0.0	0.0	0.0	0.371184707506938	0.0
4	0.0	0.0	0.0	0.6631475926925791	0.0
5	0.0	0.0	0.0	1.2689507739972041	0.0
6	0.0	0.0	0.0	1.827403681499023	0.0
7	0.0	0.0	0.0	2.1899349284139427	0.0
8	0.0	0.0	0.0	2.9426641201929113	0.0
9	0.0	0.0	0.0	3.283378896037812	0.0
10	0.0	0.0	0.0	3.8704735393019463	0.0
11	0.0	0.0	0.0	4.648005255453813	0.0
12	0.0	0.0	0.0	5.230102829925373	0.0
13	0.0	0.0	0.0	5.440528177983403	0.0
14	0.0	0.0	0.0	5.519689060441375	0.0
15	0.0	0.0	0.0	5.631148070461108	0.0
16	0.0	0.0	0.0	5.90592591418936	0.0
17	0.0	0.0	0.0	6.223910121014381	0.0
18	0.0	0.0	0.0	6.728492189337718	0.0
19	0.0	0.0	0.0	6.965792017121664	0.0
20	0.0	0.0	0.0	7.259034639437111	0.0
21	0.0	0.0	0.0	7.6291833692675395	0.0
22	0.0	0.0	0.0	7.983609614360357	0.0
23	0.0	0.0	0.0	8.372345002504629	0.0
24	0.0	0.0	0.0	8.666136083589992	0.0
25	0.0	0.0	0.0	8.919280275838197	0.0
26	0.0	0.0	0.0	9.194850337789662	0.0
27	0.0	0.0	0.0	9.549825041652397	0.0
28	0.0	0.0	0.0	9.840386309981584	0.0
29	0.0	0.0	0.0	10.219127560540707	0.0
30	0.0	0.0	0.0	10.610239603354618	0.0
31	0.0	0.0	0.0	10.967834721340287	0.0
32	0.0	0.0	0.0	11.287464304475058	0.0
33	0.0	0.0	0.0	11.611664377359135	0.0
34	0.0	0.0	0.0	11.946407046598276	0.0
35	0.0	0.0	0.0	12.351352438386751	0.0
36	0.0	0.0	0.0	12.662694200109447	0.0
37	0.0	0.0	0.0	12.98713803244682	0.0
38	0.0	0.0	0.0	13.391169326285436	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGTA	30	2.5283825E-6	44.0	31
CTAGACG	40	8.3164196E-9	44.0	1
AATCCGG	35	1.4465877E-7	44.0	2
ATTAGCG	50	2.7284841E-11	44.0	1
CGCATCG	25	4.4436747E-5	44.0	21
ATAGACG	60	0.0	44.0	1
CGATTCG	30	2.5283825E-6	44.0	10
CACGACG	115	0.0	44.0	26
CTGCGTA	25	4.4436747E-5	44.0	19
ACGATTC	20	7.8574684E-4	44.0	17
CCGTAGG	20	7.8574684E-4	44.0	2
TACGATG	40	8.3164196E-9	44.0	1
CATACGA	190	0.0	44.0	18
ATGCGTC	20	7.8574684E-4	44.0	11
CATCGGC	20	7.8574684E-4	44.0	28
GCGTGTA	30	2.5283825E-6	44.0	14
ACCGTAT	20	7.8574684E-4	44.0	35
ATCGGTT	160	0.0	44.0	2
ATCGGCC	20	7.8574684E-4	44.0	29
GTAGTAT	30	2.5283825E-6	44.0	15
>>END_MODULE