FastQCFastQC Report
Thu 26 May 2016
SRR1546229_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546229_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences930909
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130441.401211074337019No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC32200.3458984712791476No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCC29530.3172168278532059No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23310.2504004150781655No Hit
GAATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTT19840.21312502081299028No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA13870.14899415517521045No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATG12940.13900391982460156No Hit
CCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG12200.13105470029831057Illumina PCR Primer Index 10 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGC12090.12987305955791598No Hit
ATTTAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT12020.12912110635948304No Hit
GATTTGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT11850.1272949343061459No Hit
ACGAAAGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA11630.12493165282535673No Hit
TAGTGAGGGGGCTATTAATATCTTATCATTTGGTTTTTAATATTCGATAA11580.12439454339790462No Hit
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC11410.12256837134456751Illumina PCR Primer Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG11000.11816407403946036Illumina PCR Primer Index 10 (95% over 22bp)
GGTAGAGGGAGTTTATCTTAGGGGCTATTAATATCTTATCATTTGGTTTT10920.117304698955537No Hit
TTGGTGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA10890.11698243329906574No Hit
AGTTAAGGGGGGGCTATTAATATCTTATCATTTGGTTTTTAATATTCGAT10780.11580079255867114No Hit
ACGTAAGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATT10470.11247071410846816No Hit
GATCTTGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA10090.10838868245983226No Hit
GTTTCTGGGAAGATGATGATGATGAATAAGTTGGTTCTAGCGCAGTTTTT9910.10645508852100474No Hit
AGAAGCGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC9830.10559571343708139No Hit
GAGCTAGGGGTTTTTAACCATAATTACATTTCAAATCTTCATTTCTTTTT9820.10548829155159097No Hit
TTGAATGGGAATCTAAGTATAGGTAATATAACAATTAAAAAGATTTAGAG9790.10516602589511971No Hit
TACAGGGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATC9680.1039843851547251No Hit
TTTGAAGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTA9500.1020507912158976No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAC502.7284841E-1144.025
TTTAGCG408.309144E-944.01
TATCACT207.8557094E-444.010
GTGCACG207.8557094E-444.01
CGGGTCA502.7284841E-1144.06
CGGGTAG254.442186E-544.06
TAATACG207.8557094E-444.01
GCCCATC207.8557094E-444.026
ACTGCGC207.8557094E-444.019
TCACCGT254.442186E-544.022
GATCGTC254.442186E-544.09
TCCGCAA302.5271966E-644.032
CGAGTTA207.8557094E-444.035
ATCTAGT254.442186E-544.027
GTCGACG207.8557094E-444.01
GTACACG254.442186E-544.01
GTCGAAC207.8557094E-444.034
CTCCGCA302.5271966E-644.022
ACGTTTG207.8557094E-444.01
CACGGGT551.8189894E-1244.04
Produced by FastQC (version 0.11.5)