##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546229_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	930909
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.823825959358004	31.0	31.0	34.0	30.0	34.0
2	32.17264738014135	33.0	31.0	34.0	30.0	34.0
3	32.04598945761616	33.0	31.0	34.0	30.0	34.0
4	35.83098992490136	37.0	35.0	37.0	35.0	37.0
5	35.816063653912465	37.0	35.0	37.0	35.0	37.0
6	35.862272252175025	37.0	35.0	37.0	35.0	37.0
7	36.13242540355717	37.0	35.0	37.0	35.0	37.0
8	36.01215908321866	37.0	35.0	37.0	35.0	37.0
9	37.77369538805619	39.0	38.0	39.0	35.0	39.0
10	37.42742953392867	39.0	37.0	39.0	34.0	39.0
11	37.229172776286404	39.0	37.0	39.0	34.0	39.0
12	36.39279779226541	38.0	35.0	39.0	33.0	39.0
13	36.01205058711432	38.0	35.0	39.0	32.0	39.0
14	37.0859074302644	39.0	35.0	41.0	32.0	41.0
15	37.36130276965847	39.0	35.0	41.0	33.0	41.0
16	37.511171338981576	39.0	35.0	41.0	33.0	41.0
17	37.44109789463847	39.0	35.0	41.0	33.0	41.0
18	37.24274338308041	39.0	35.0	40.0	33.0	41.0
19	36.94528895950088	37.0	35.0	40.0	33.0	41.0
20	36.56511109034288	36.0	35.0	40.0	33.0	41.0
21	36.46455561177301	36.0	35.0	40.0	32.0	41.0
22	36.37034984085447	35.0	35.0	40.0	32.0	41.0
23	36.30707620186291	35.0	35.0	40.0	32.0	41.0
24	36.24123517980812	35.0	35.0	40.0	32.0	41.0
25	36.268753444214205	35.0	35.0	40.0	32.0	41.0
26	36.17106935264349	35.0	35.0	40.0	32.0	41.0
27	36.074801081523546	35.0	35.0	40.0	32.0	41.0
28	36.00522607472911	36.0	35.0	40.0	31.0	41.0
29	35.94744706518038	36.0	35.0	40.0	31.0	41.0
30	35.707579365974546	36.0	35.0	40.0	31.0	41.0
31	35.31687200360078	36.0	35.0	40.0	30.0	41.0
32	34.84369578551717	35.0	34.0	40.0	26.0	41.0
33	34.31754876147937	35.0	34.0	40.0	23.0	41.0
34	33.786790115897475	35.0	34.0	40.0	18.0	41.0
35	33.474045261137235	35.0	34.0	40.0	16.0	41.0
36	33.26637834632601	35.0	33.0	40.0	15.0	41.0
37	33.213809298223566	35.0	33.0	40.0	15.0	41.0
38	33.18534357278746	35.0	33.0	40.0	15.0	41.0
39	33.13735929075774	35.0	33.0	40.0	15.0	41.0
40	32.93448876313367	35.0	33.0	40.0	15.0	41.0
41	32.81304295049247	35.0	33.0	40.0	12.0	41.0
42	32.703804560918414	35.0	33.0	40.0	10.0	41.0
43	32.5519186085858	35.0	32.0	40.0	10.0	41.0
44	32.420275236355	35.0	32.0	40.0	10.0	41.0
45	32.33366097008408	35.0	32.0	40.0	10.0	41.0
46	32.287356766343436	35.0	32.0	40.0	10.0	41.0
47	32.23460724947336	35.0	32.0	40.0	10.0	41.0
48	32.195603437070645	35.0	32.0	40.0	10.0	41.0
49	32.16811202813594	35.0	32.0	40.0	10.0	41.0
50	31.942051263872195	35.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	0.0
13	2.0
14	12.0
15	19.0
16	74.0
17	187.0
18	414.0
19	870.0
20	1674.0
21	2701.0
22	4277.0
23	6727.0
24	10613.0
25	17335.0
26	27251.0
27	33199.0
28	31048.0
29	26531.0
30	24856.0
31	25737.0
32	30104.0
33	39194.0
34	74258.0
35	162308.0
36	47318.0
37	56616.0
38	91964.0
39	215619.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.418791740116383	23.994719140109293	26.998449902192373	21.588039217581954
2	36.380247693383566	24.596926230168577	28.180950017670902	10.84187605877696
3	23.161232730589134	23.988273826979867	41.88873455944673	10.961758882984265
4	21.211418087052547	25.83292244462133	41.30865637779848	11.647003090527646
5	19.79291208915157	28.90712196358613	40.51749419116154	10.782471756100758
6	16.53996255273072	38.48582407088126	35.7430210686544	9.231192307733624
7	74.61953853706432	3.532461282466922	20.122160168179704	1.7258400122890636
8	74.50094477548289	3.930996477636375	18.81236511839503	2.755693628485706
9	71.53803432988617	4.39452191352753	20.341193392694667	3.7262503638916376
10	45.38016068165632	18.721701047041115	25.625383361853842	10.272754909448722
11	39.94998437011566	17.467443112054994	31.283186648748696	11.299385869080652
12	35.61293316532551	16.94612470176999	36.27357776109158	11.167364371812926
13	15.158731949094918	38.806263555299175	35.31301126103625	10.721993234569652
14	9.19166105387315	41.58161538882963	38.018538868997936	11.208184688299285
15	8.273526198586541	20.687736395286755	60.42985941697846	10.60887798914824
16	9.644336879329774	17.991661913248233	55.386831580745266	16.977169626676723
17	9.456563423492522	18.10875176843279	39.2085585164608	33.226126291613895
18	13.594347030697953	21.611134922962393	47.365102281748264	17.429415764591383
19	19.534669876432606	21.14051964262887	39.32317766827907	20.00163281265945
20	20.94243368578454	20.945226654807293	40.40394925819817	17.70839040121
21	16.464230123459973	24.793186014959574	42.142465052975105	16.60011880860535
22	18.259357251890357	21.703410322598664	35.02232763889918	25.014904786611798
23	13.374884118641027	25.406994668651823	35.8192906073526	25.39883060535455
24	12.84411258243287	22.16478732078001	50.67874518347121	14.31235491331591
25	10.785049881352528	24.016633204749336	47.5183933123431	17.67992360155504
26	12.166387906873819	28.451653169106756	39.209955000972165	20.172003923047257
27	12.953468061862116	35.76783552420269	33.81716150558218	17.46153490835302
28	9.596104452744575	30.929446379828747	42.801928008000786	16.672521159425894
29	11.45332143098842	22.49575414997599	41.12152745327416	24.929396965761423
30	12.81833132991517	31.642835121370616	37.746976342478156	17.791857206236056
31	18.27890803504961	30.17555958745699	35.37907572061286	16.16645665688053
32	22.02879121374914	23.897287489969482	37.977288864969616	16.09663243131176
33	20.198322284992408	28.44531527786282	33.11934893743642	18.23701349970835
34	14.940450677778387	28.228108225401193	34.301419365372986	22.530021731447437
35	16.236066038678324	24.738615697130438	35.31268899537979	23.71262926881145
36	26.31503186670233	23.800285527371635	32.79547195268281	17.089210653243228
37	14.853546372416638	30.801936601751624	36.82132195520722	17.52319507062452
38	15.89317538019291	29.16966105172471	33.723704465205515	21.213459102876868
39	16.10135899427334	30.464417037540727	35.060892095790244	18.37333187239569
40	19.70998239355297	23.23610578477596	32.94811845196469	24.105793369706383
41	13.58317515460695	25.502170459196332	34.39036468655905	26.524289699637666
42	17.925167766129665	24.804357891050575	32.720706320381474	24.549768022438283
43	17.470988034276175	23.20720929757903	33.968196676581705	25.353605991563082
44	15.175597185116912	27.51568628082874	33.688684930535636	23.62003160351871
45	15.21641750160327	35.11213233516917	28.81538367337731	20.856066489850242
46	21.03717978878709	29.750813452227874	31.918049991997073	17.293956766987968
47	17.50590014706056	29.1619266759694	31.88979803611309	21.44237514085695
48	18.07115410851114	26.184621697716963	34.20613615294298	21.53808804082891
49	20.400275429714398	23.30700422919963	36.09665391568886	20.19606642539711
50	18.145167787614042	28.607737168724334	31.69998356445152	21.547111479210106
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2703.0
1	3124.5
2	3546.0
3	13241.5
4	22937.0
5	17541.0
6	12145.0
7	13499.5
8	14854.0
9	16271.5
10	17689.0
11	17576.0
12	17463.0
13	16274.0
14	15085.0
15	13449.5
16	11814.0
17	10524.0
18	9234.0
19	8455.0
20	7676.0
21	7370.5
22	7065.0
23	7583.0
24	8101.0
25	8117.5
26	8134.0
27	12918.0
28	17702.0
29	17573.5
30	17445.0
31	20169.0
32	22893.0
33	28687.5
34	34482.0
35	40217.5
36	45953.0
37	47195.0
38	48437.0
39	54808.5
40	61180.0
41	68703.5
42	76227.0
43	86403.5
44	96580.0
45	95271.5
46	93963.0
47	83077.5
48	72192.0
49	64435.0
50	56678.0
51	45159.0
52	33640.0
53	28104.0
54	22568.0
55	19680.0
56	16792.0
57	15081.5
58	13371.0
59	12356.5
60	11342.0
61	10674.0
62	10006.0
63	8503.5
64	7001.0
65	6090.5
66	5180.0
67	4153.0
68	3126.0
69	2662.5
70	2199.0
71	1958.5
72	1718.0
73	1239.0
74	760.0
75	658.5
76	557.0
77	401.0
78	245.0
79	184.0
80	123.0
81	99.0
82	75.0
83	44.5
84	14.0
85	9.0
86	4.0
87	3.5
88	3.0
89	2.0
90	1.0
91	1.5
92	2.0
93	2.5
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	930909.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.78106936938142
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.03891055038154	20.377076357797197
2	8.876237563221377	4.576777927130388
3	3.2614346156840495	2.5224981621195695
4	1.712103605795024	1.765594473142783
5	1.0238361313544073	1.3197795162663541
6	0.7073871925269378	1.0942318968929372
7	0.511312259662579	0.922752378504328
8	0.38214093106091984	0.7881601482049262
9	0.3177912813620923	0.7373699162802638
>10	3.0150899573803343	17.7449977333641
>50	0.6396275521678776	11.360499513220823
>100	0.45639873076365095	23.797558853348917
>500	0.049363015792952165	8.580986397744221
>1k	0.007948282203949926	3.004919850861564
>5k	0.0	0.0
>10k+	4.1833064231315396E-4	1.406796875121608
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13044	1.401211074337019	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	3220	0.3458984712791476	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCC	2953	0.3172168278532059	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2331	0.2504004150781655	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTT	1984	0.21312502081299028	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1387	0.14899415517521045	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATG	1294	0.13900391982460156	No Hit
CCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG	1220	0.13105470029831057	Illumina PCR Primer Index 10 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGC	1209	0.12987305955791598	No Hit
ATTTAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1202	0.12912110635948304	No Hit
GATTTGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1185	0.1272949343061459	No Hit
ACGAAAGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA	1163	0.12493165282535673	No Hit
TAGTGAGGGGGCTATTAATATCTTATCATTTGGTTTTTAATATTCGATAA	1158	0.12439454339790462	No Hit
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	1141	0.12256837134456751	Illumina PCR Primer Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG	1100	0.11816407403946036	Illumina PCR Primer Index 10 (95% over 22bp)
GGTAGAGGGAGTTTATCTTAGGGGCTATTAATATCTTATCATTTGGTTTT	1092	0.117304698955537	No Hit
TTGGTGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA	1089	0.11698243329906574	No Hit
AGTTAAGGGGGGGCTATTAATATCTTATCATTTGGTTTTTAATATTCGAT	1078	0.11580079255867114	No Hit
ACGTAAGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATT	1047	0.11247071410846816	No Hit
GATCTTGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA	1009	0.10838868245983226	No Hit
GTTTCTGGGAAGATGATGATGATGAATAAGTTGGTTCTAGCGCAGTTTTT	991	0.10645508852100474	No Hit
AGAAGCGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	983	0.10559571343708139	No Hit
GAGCTAGGGGTTTTTAACCATAATTACATTTCAAATCTTCATTTCTTTTT	982	0.10548829155159097	No Hit
TTGAATGGGAATCTAAGTATAGGTAATATAACAATTAAAAAGATTTAGAG	979	0.10516602589511971	No Hit
TACAGGGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATC	968	0.1039843851547251	No Hit
TTTGAAGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTA	950	0.1020507912158976	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14491212352657457	0.0
2	0.0	0.0	0.0	0.6108008408985196	0.0
3	0.0	0.0	0.0	0.8770996950292671	0.0
4	0.0	0.0	0.0	1.3757520874757898	0.0
5	0.0	0.0	0.0	2.498740478392625	0.0
6	0.0	0.0	0.0	3.2928030559377985	0.0
7	0.0	0.0	0.0	3.8257230298557645	0.0
8	0.0	0.0	0.0	4.91551805815606	0.0
9	0.0	0.0	0.0	5.252393091054013	0.0
10	0.0	0.0	0.0	6.158818960822164	0.0
11	0.0	0.0	0.0	6.883057312798566	0.0
12	0.0	0.0	0.0	7.583340584310604	0.0
13	1.0742188549041851E-4	0.0	0.0	7.815479278855398	0.0
14	1.0742188549041851E-4	0.0	0.0	7.8977644431410585	0.0
15	1.0742188549041851E-4	0.0	0.0	8.067920709757882	0.0
16	1.0742188549041851E-4	0.0	0.0	8.37772542751225	0.0
17	1.0742188549041851E-4	0.0	0.0	8.75241296410283	0.0
18	1.0742188549041851E-4	0.0	0.0	9.361280211062521	0.0
19	1.0742188549041851E-4	0.0	0.0	9.631446253070923	0.0
20	1.0742188549041851E-4	0.0	0.0	9.899893544911478	0.0
21	1.0742188549041851E-4	0.0	0.0	10.284141629310707	0.0
22	1.0742188549041851E-4	0.0	0.0	10.630147522475344	0.0
23	1.0742188549041851E-4	0.0	0.0	11.078633894397841	0.0
24	1.0742188549041851E-4	0.0	0.0	11.35803821855842	0.0
25	1.0742188549041851E-4	0.0	0.0	11.601348789194217	0.0
26	1.0742188549041851E-4	0.0	0.0	11.910831241292113	0.0
27	1.0742188549041851E-4	0.0	0.0	12.150919155363198	0.0
28	1.0742188549041851E-4	0.0	0.0	12.426348869760632	0.0
29	1.0742188549041851E-4	0.0	0.0	12.678897722548605	0.0
30	1.0742188549041851E-4	0.0	0.0	13.091934872259264	0.0
31	1.0742188549041851E-4	0.0	0.0	13.494874364733825	0.0
32	1.0742188549041851E-4	0.0	0.0	13.818536505716455	0.0
33	1.0742188549041851E-4	0.0	0.0	14.0994447362739	0.0
34	1.0742188549041851E-4	0.0	0.0	14.37917132609095	0.0
35	1.0742188549041851E-4	0.0	0.0	14.811974102731845	0.0
36	1.0742188549041851E-4	0.0	0.0	15.145304213408615	0.0
37	1.0742188549041851E-4	0.0	0.0	15.473263229810863	0.0
38	1.0742188549041851E-4	0.0	0.0	15.765021070802838	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	50	2.7284841E-11	44.0	25
TTTAGCG	40	8.309144E-9	44.0	1
TATCACT	20	7.8557094E-4	44.0	10
GTGCACG	20	7.8557094E-4	44.0	1
CGGGTCA	50	2.7284841E-11	44.0	6
CGGGTAG	25	4.442186E-5	44.0	6
TAATACG	20	7.8557094E-4	44.0	1
GCCCATC	20	7.8557094E-4	44.0	26
ACTGCGC	20	7.8557094E-4	44.0	19
TCACCGT	25	4.442186E-5	44.0	22
GATCGTC	25	4.442186E-5	44.0	9
TCCGCAA	30	2.5271966E-6	44.0	32
CGAGTTA	20	7.8557094E-4	44.0	35
ATCTAGT	25	4.442186E-5	44.0	27
GTCGACG	20	7.8557094E-4	44.0	1
GTACACG	25	4.442186E-5	44.0	1
GTCGAAC	20	7.8557094E-4	44.0	34
CTCCGCA	30	2.5271966E-6	44.0	22
ACGTTTG	20	7.8557094E-4	44.0	1
CACGGGT	55	1.8189894E-12	44.0	4
>>END_MODULE