FastQCFastQC Report
Thu 26 May 2016
SRR1546228_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546228_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences956640
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138081.443385181468473No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC33340.3485114567653454No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCC29410.3074301722696103No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA24400.25505937447733734No Hit
GAATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTT19930.20833333333333334No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA13920.14550928248871048No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATG13580.14195517645091152No Hit
CCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG12930.13516056196688409Illumina PCR Primer Index 10 (95% over 22bp)
GATTTGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT12520.13087472821542062No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGC12280.12836594748285665No Hit
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG12040.12585716675029268Illumina PCR Primer Index 10 (95% over 22bp)
ATTTAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT11840.1237665161398227No Hit
ACGAAAGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA11830.12366198360929921No Hit
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC11570.12094413781568825Illumina PCR Primer Index 10 (95% over 23bp)
ACGTAAGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATT11530.12052600769359426No Hit
TAGTGAGGGGGCTATTAATATCTTATCATTTGGTTTTTAATATTCGATAA11400.11916708479678877No Hit
GGTAGAGGGAGTTTATCTTAGGGGCTATTAATATCTTATCATTTGGTTTT11180.11686736912527179No Hit
AGTTAAGGGGGGGCTATTAATATCTTATCATTTGGTTTTTAATATTCGAT10810.11299966549590233No Hit
TTGGTGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA10660.11143167753804985No Hit
TTTGAAGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTA10310.10777303896972738No Hit
GTTTCTGGGAAGATGATGATGATGAATAAGTTGGTTCTAGCGCAGTTTTT10260.10725037631710989No Hit
GAGCTAGGGGTTTTTAACCATAATTACATTTCAAATCTTCATTTCTTTTT9990.1044279979929754No Hit
TGGTGCGGGGATTTAAGAACATACTAGACTCATTGTCTTCCAGTATATTT9980.10432346546245191No Hit
TTTTAAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG9930.10380080280983442No Hit
AGAAGCGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC9930.10380080280983442No Hit
TACAGGGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATC9840.10286001003512292No Hit
TAGAGAGGGAATGCAGAATGATTGAGGAGATGATAAAATCAAAATATTTA9780.10223281485198193No Hit
TTGAATGGGAATCTAAGTATAGGTAATATAACAATTAAAAAGATTTAGAG9780.10223281485198193No Hit
GTCTCAGGGATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATT9730.10171015219936444No Hit
TGTAAAGGGACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTA9570.10003763171098846No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGCAAC302.5272693E-644.00000441
ACAGTCC600.044.00000416
GATGTGC302.5272693E-644.00000425
GGTAATC302.5272693E-644.00000420
GTCGACG302.5272693E-644.0000041
AGATAAC302.5272693E-644.00000438
GTAATCG302.5272693E-644.00000421
TCTACCC302.5272693E-644.00000432
TGATTCG302.5272693E-644.0000042
GTGTACG302.5272693E-644.0000041
TGTTACG302.5272693E-644.0000041
TCGTACC302.5272693E-644.00000430
TACTAAC600.044.00000431
CGTTTAG302.5272693E-644.00000430
CAGAGTT302.5272693E-644.00000413
AAGCTCG302.5272693E-644.00000419
GGCTAAC302.5272693E-644.0000048
TATCATG454.802132E-1044.035
GTTTGCG408.309144E-944.01
GTCATAG207.855819E-444.01
Produced by FastQC (version 0.11.5)