##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546228_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	956640
Sequences flagged as poor quality	0
Sequence length	50
%GC	39
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.764134888777388	31.0	31.0	34.0	30.0	34.0
2	32.15251296203378	33.0	31.0	34.0	30.0	34.0
3	32.15409872052182	33.0	31.0	34.0	30.0	34.0
4	35.86718410269276	37.0	35.0	37.0	35.0	37.0
5	35.77998933768189	37.0	35.0	37.0	35.0	37.0
6	35.856951413279816	37.0	35.0	37.0	35.0	37.0
7	36.121518021408264	37.0	35.0	37.0	35.0	37.0
8	36.00364818531527	37.0	35.0	37.0	35.0	37.0
9	37.7570893962201	39.0	38.0	39.0	35.0	39.0
10	37.42825409767519	39.0	37.0	39.0	34.0	39.0
11	37.23623933768189	39.0	37.0	39.0	34.0	39.0
12	36.39001191670848	38.0	35.0	39.0	33.0	39.0
13	35.95045262585717	38.0	35.0	39.0	32.0	39.0
14	37.01719873724703	39.0	35.0	41.0	32.0	41.0
15	37.32895446562971	39.0	35.0	41.0	33.0	41.0
16	37.47651258571668	39.0	35.0	41.0	33.0	41.0
17	37.41343033952166	39.0	35.0	41.0	33.0	41.0
18	37.23353821709316	39.0	35.0	40.0	33.0	41.0
19	36.94674067569828	37.0	35.0	40.0	33.0	41.0
20	36.563856832246195	36.0	35.0	40.0	33.0	41.0
21	36.45969016557953	36.0	35.0	40.0	32.0	41.0
22	36.37047478675364	35.0	35.0	40.0	32.0	41.0
23	36.29187886770363	35.0	35.0	40.0	32.0	41.0
24	36.22469790098679	35.0	35.0	40.0	32.0	41.0
25	36.235823298210406	35.0	35.0	40.0	32.0	41.0
26	36.16851793778224	35.0	35.0	40.0	32.0	41.0
27	36.054683057367455	35.0	35.0	40.0	32.0	41.0
28	35.98677558956347	36.0	35.0	40.0	31.0	41.0
29	35.92571709315939	36.0	35.0	40.0	31.0	41.0
30	35.69416290349557	36.0	35.0	40.0	31.0	41.0
31	35.31233588392708	36.0	35.0	40.0	30.0	41.0
32	34.84053667001171	35.0	34.0	40.0	26.0	41.0
33	34.30666081284496	35.0	34.0	40.0	23.0	41.0
34	33.84755916541228	35.0	34.0	40.0	18.0	41.0
35	33.51823569994983	35.0	34.0	40.0	17.0	41.0
36	33.28812301388192	35.0	33.0	40.0	15.0	41.0
37	33.17618958019736	35.0	33.0	40.0	15.0	41.0
38	33.1514373222947	35.0	33.0	40.0	15.0	41.0
39	33.11296098845961	35.0	33.0	40.0	15.0	41.0
40	32.92805653119251	35.0	33.0	40.0	15.0	41.0
41	32.83262146680047	35.0	33.0	40.0	12.0	41.0
42	32.699816022746276	35.0	33.0	40.0	10.0	41.0
43	32.575659600267606	35.0	32.0	40.0	10.0	41.0
44	32.434261582204385	35.0	32.0	40.0	10.0	41.0
45	32.35017665997658	35.0	32.0	40.0	10.0	41.0
46	32.31342093159391	35.0	32.0	40.0	10.0	41.0
47	32.23987811506941	35.0	32.0	40.0	10.0	41.0
48	32.21589626191671	35.0	32.0	40.0	10.0	41.0
49	32.19075096169928	35.0	32.0	40.0	10.0	41.0
50	31.92842239504934	35.0	31.0	40.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	1.0
13	6.0
14	13.0
15	23.0
16	71.0
17	154.0
18	455.0
19	933.0
20	1690.0
21	2822.0
22	4523.0
23	6957.0
24	10966.0
25	17828.0
26	27286.0
27	33587.0
28	32211.0
29	27681.0
30	25783.0
31	26211.0
32	31415.0
33	40837.0
34	78320.0
35	163783.0
36	49251.0
37	58254.0
38	94818.0
39	220757.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.38407342364944	24.073319116909182	27.128804984111056	21.413802475330325
2	36.241846462619165	24.717344037464457	28.219915537715334	10.820893962201037
3	23.176639070078608	23.950388861013547	41.910436527847466	10.962535541060378
4	21.267247867536376	25.83751463455427	41.316587221943465	11.578650275965881
5	19.985260913196186	28.79954841946814	40.42868790767687	10.786502759658806
6	16.51018146847299	38.45302308078274	35.66357250376317	9.373222946981102
7	74.57862936945978	3.5458479678876067	20.156380665663153	1.7191419969894632
8	74.46155293527346	3.9237330657300555	18.797353236327144	2.8173607626693427
9	71.37387104867034	4.396115571165747	20.389697273791604	3.840316106372303
10	45.335340357919385	18.69742013714668	25.633571667502924	10.333667837431008
11	39.94815186486034	17.44240257568155	31.245609633718015	11.36383592574009
12	35.49506606455929	16.937614985783576	36.301534537548086	11.265784412109047
13	15.257568155209903	38.64473574176284	35.367118247198526	10.730577855828734
14	9.253010536879076	41.50255059374477	38.03029352734571	11.21414534203044
15	8.222946981100518	20.84305485867202	60.30063555778559	10.63336260244188
16	9.66748202040475	18.081514467302224	55.25850894798461	16.992494564308412
17	9.405000836260244	18.206326308747283	39.20429837765513	33.18437447733735
18	13.563095835423983	21.596002676032782	47.35459525004181	17.486306238501424
19	19.453922060545242	21.138150192339854	39.35033032279646	20.05759742431845
20	20.971420806154875	20.948214584378658	40.366804649606955	17.71355995985951
21	16.477462786419135	24.781422478675363	42.099640408095	16.641474326810503
22	18.257547248703794	21.691649941461783	35.01536628198696	25.035436527847466
23	13.385076935942466	25.304712326475997	35.84660896470982	25.46360177287172
24	12.914157885934102	22.09514550928249	50.65719601940124	14.333500585382172
25	10.726919217260411	24.084190500083626	47.537527178457935	17.651363104198026
26	12.095250041813012	28.47946981100519	39.190395551095506	20.234884596086303
27	12.934855326977758	35.70831242682723	33.854009867870886	17.502822378324133
28	9.632359090148853	30.96378993142666	42.72077270446563	16.683078273958856
29	11.405858003010536	22.640387188493058	40.95155962535541	25.002195183140998
30	12.851229302558956	31.558684562635893	37.664847800635556	17.925238334169595
31	18.155000836260243	30.242515470814517	35.31432931928416	16.288154373641078
32	21.981309583542398	23.906171600602107	37.971546245191504	16.14097257066399
33	20.139969058370966	28.435148018063224	33.132944472319785	18.29193845124603
34	14.954841946813849	28.146115571165748	34.251128951329655	22.64791353069075
35	16.18184479009868	24.709190500083626	35.20247951162402	23.90648519819368
36	26.358504766683392	23.621634052517145	32.71544154540893	17.304419635390534
37	14.844141996989462	30.730159725706642	36.85012125773541	17.575577019568488
38	15.903579193845124	29.155899816022746	33.78857250376317	21.15194848636896
39	16.1557116574678	30.273352567318952	35.11237247031276	18.458563304900487
40	19.657655962535543	23.17193510620505	33.04116491052015	24.129244020739254
41	13.547520488375984	25.421684228131795	34.430611306238504	26.60018397725372
42	17.97468222110721	24.773687071416624	32.67551011874896	24.576120588727214
43	17.507944472319785	23.114024084295032	33.9381585549423	25.43987288844288
44	15.175719183810003	27.51484361933434	33.600832078942965	23.708605117912697
45	15.198507275464124	34.95693259742432	28.846169928081615	20.998390199029938
46	21.0827479511624	29.593891118916204	31.909704800133802	17.41365612978759
47	17.45703712995484	29.135411440040144	31.917022077270445	21.490529352734573
48	17.969873724703127	26.134177956179965	34.20440291018565	21.69154540893126
49	20.36157802308078	23.231832246195015	36.04825221608965	20.358337514634552
50	18.245630540224116	28.515847131627364	31.688304900485033	21.55021742766349
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2792.0
1	3255.5
2	3719.0
3	13867.0
4	24015.0
5	18009.5
6	12004.0
7	13409.0
8	14814.0
9	16310.5
10	17807.0
11	17754.0
12	17701.0
13	16719.5
14	15738.0
15	13938.0
16	12138.0
17	10817.0
18	9496.0
19	8711.5
20	7927.0
21	7573.0
22	7219.0
23	7756.0
24	8293.0
25	8230.5
26	8168.0
27	13197.5
28	18227.0
29	18011.5
30	17796.0
31	20571.5
32	23347.0
33	29395.0
34	35443.0
35	41265.5
36	47088.0
37	48346.0
38	49604.0
39	56091.0
40	62578.0
41	70732.5
42	78887.0
43	88860.5
44	98834.0
45	97599.0
46	96364.0
47	85660.5
48	74957.0
49	67050.0
50	59143.0
51	47086.0
52	35029.0
53	29401.5
54	23774.0
55	20392.0
56	17010.0
57	15503.5
58	13997.0
59	12833.5
60	11670.0
61	10815.0
62	9960.0
63	8471.5
64	6983.0
65	6067.5
66	5152.0
67	4130.0
68	3108.0
69	2649.0
70	2190.0
71	1989.0
72	1788.0
73	1319.5
74	851.0
75	689.5
76	528.0
77	383.0
78	238.0
79	189.5
80	141.0
81	110.5
82	80.0
83	53.5
84	27.0
85	16.0
86	5.0
87	4.0
88	3.0
89	1.5
90	0.0
91	0.5
92	1.0
93	3.5
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	956640.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	25.748673264184447
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.69014527709857	20.261668398512175
2	8.905077997767657	4.585878875131945
3	3.4828311102290073	2.690348408748704
4	1.7618562059834582	1.8146183914537488
5	1.0609906341128708	1.3659550587066087
6	0.7198882780490404	1.1121700834920663
7	0.542088658430531	0.9770644622303482
8	0.3977280284598573	0.8192775242256892
9	0.305208427360479	0.7072840866222491
>10	2.974141628499248	17.265667904084268
>50	0.6468690927197754	11.417403219411518
>100	0.45407194017195296	23.640319731497623
>500	0.05054301667982241	8.789278329630443
>1k	0.008152099464487484	3.1038762591258076
>5k	0.0	0.0
>10k+	4.0760497322437424E-4	1.4491892671268394
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13808	1.443385181468473	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	3334	0.3485114567653454	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCC	2941	0.3074301722696103	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2440	0.25505937447733734	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTT	1993	0.20833333333333334	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1392	0.14550928248871048	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATG	1358	0.14195517645091152	No Hit
CCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG	1293	0.13516056196688409	Illumina PCR Primer Index 10 (95% over 22bp)
GATTTGGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1252	0.13087472821542062	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGC	1228	0.12836594748285665	No Hit
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG	1204	0.12585716675029268	Illumina PCR Primer Index 10 (95% over 22bp)
ATTTAAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1184	0.1237665161398227	No Hit
ACGAAAGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA	1183	0.12366198360929921	No Hit
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	1157	0.12094413781568825	Illumina PCR Primer Index 10 (95% over 23bp)
ACGTAAGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAATT	1153	0.12052600769359426	No Hit
TAGTGAGGGGGCTATTAATATCTTATCATTTGGTTTTTAATATTCGATAA	1140	0.11916708479678877	No Hit
GGTAGAGGGAGTTTATCTTAGGGGCTATTAATATCTTATCATTTGGTTTT	1118	0.11686736912527179	No Hit
AGTTAAGGGGGGGCTATTAATATCTTATCATTTGGTTTTTAATATTCGAT	1081	0.11299966549590233	No Hit
TTGGTGAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA	1066	0.11143167753804985	No Hit
TTTGAAGGGTATGCCACAACTAGATACATCAACATGATTTATCACAATTA	1031	0.10777303896972738	No Hit
GTTTCTGGGAAGATGATGATGATGAATAAGTTGGTTCTAGCGCAGTTTTT	1026	0.10725037631710989	No Hit
GAGCTAGGGGTTTTTAACCATAATTACATTTCAAATCTTCATTTCTTTTT	999	0.1044279979929754	No Hit
TGGTGCGGGGATTTAAGAACATACTAGACTCATTGTCTTCCAGTATATTT	998	0.10432346546245191	No Hit
TTTTAAGGGGGGAATTCTCTTGCTTCAACAATAACGTCTCTTTCAGAAGG	993	0.10380080280983442	No Hit
AGAAGCGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	993	0.10380080280983442	No Hit
TACAGGGGTATCCCAATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATC	984	0.10286001003512292	No Hit
TAGAGAGGGAATGCAGAATGATTGAGGAGATGATAAAATCAAAATATTTA	978	0.10223281485198193	No Hit
TTGAATGGGAATCTAAGTATAGGTAATATAACAATTAAAAAGATTTAGAG	978	0.10223281485198193	No Hit
GTCTCAGGGATGGTTTTCAAAACAATCACCATCATGCTATTAATGATATT	973	0.10171015219936444	No Hit
TGTAAAGGGACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTA	957	0.10003763171098846	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14331409934771702	0.0
2	0.0	0.0	0.0	0.6292858337514634	0.0
3	0.0	0.0	0.0	0.9015930757651781	0.0
4	0.0	0.0	0.0	1.405021742766349	0.0
5	0.0	0.0	0.0	2.5536251881585548	0.0
6	0.0	0.0	0.0	3.3873766516139825	0.0
7	0.0	0.0	0.0	3.923942130791102	0.0
8	0.0	0.0	0.0	5.041290349556782	0.0
9	0.0	0.0	0.0	5.413112560628868	0.0
10	0.0	0.0	0.0	6.313869376149857	0.0
11	0.0	0.0	0.0	7.064203880247533	0.0
12	0.0	0.0	0.0	7.780878909516642	0.0
13	0.0	0.0	0.0	8.019317611640743	0.0
14	0.0	0.0	0.0	8.104825221608964	0.0
15	0.0	0.0	0.0	8.278976417461115	0.0
16	0.0	0.0	0.0	8.60574510787757	0.0
17	0.0	0.0	0.0	9.011958521491888	0.0
18	0.0	0.0	0.0	9.672708646930925	0.0
19	0.0	0.0	0.0	9.946374811841444	0.0
20	0.0	0.0	0.0	10.230180632212745	0.0
21	1.0453253052349892E-4	0.0	0.0	10.62562719518314	0.0
22	1.0453253052349892E-4	0.0	0.0	10.984905502592406	0.0
23	1.0453253052349892E-4	0.0	0.0	11.452479511624018	0.0
24	1.0453253052349892E-4	0.0	0.0	11.751756146512795	0.0
25	1.0453253052349892E-4	0.0	0.0	12.01151948486369	0.0
26	1.0453253052349892E-4	0.0	0.0	12.32041311256063	0.0
27	1.0453253052349892E-4	0.0	0.0	12.56543736410771	0.0
28	1.0453253052349892E-4	0.0	0.0	12.845480013380165	0.0
29	1.0453253052349892E-4	0.0	0.0	13.112978758989797	0.0
30	1.0453253052349892E-4	0.0	0.0	13.530377153370129	0.0
31	1.0453253052349892E-4	0.0	0.0	13.953315771868205	0.0
32	1.0453253052349892E-4	0.0	0.0	14.289805987623348	0.0
33	1.0453253052349892E-4	0.0	0.0	14.580720020070245	0.0
34	1.0453253052349892E-4	0.0	0.0	14.866825556113062	0.0
35	1.0453253052349892E-4	0.0	0.0	15.317778892791436	0.0
36	1.0453253052349892E-4	0.0	0.0	15.65698695434019	0.0
37	1.0453253052349892E-4	0.0	0.0	15.996822211072086	0.0
38	1.0453253052349892E-4	0.0	0.0	16.297666833918715	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGCAAC	30	2.5272693E-6	44.000004	41
ACAGTCC	60	0.0	44.000004	16
GATGTGC	30	2.5272693E-6	44.000004	25
GGTAATC	30	2.5272693E-6	44.000004	20
GTCGACG	30	2.5272693E-6	44.000004	1
AGATAAC	30	2.5272693E-6	44.000004	38
GTAATCG	30	2.5272693E-6	44.000004	21
TCTACCC	30	2.5272693E-6	44.000004	32
TGATTCG	30	2.5272693E-6	44.000004	2
GTGTACG	30	2.5272693E-6	44.000004	1
TGTTACG	30	2.5272693E-6	44.000004	1
TCGTACC	30	2.5272693E-6	44.000004	30
TACTAAC	60	0.0	44.000004	31
CGTTTAG	30	2.5272693E-6	44.000004	30
CAGAGTT	30	2.5272693E-6	44.000004	13
AAGCTCG	30	2.5272693E-6	44.000004	19
GGCTAAC	30	2.5272693E-6	44.000004	8
TATCATG	45	4.802132E-10	44.0	35
GTTTGCG	40	8.309144E-9	44.0	1
GTCATAG	20	7.855819E-4	44.0	1
>>END_MODULE