FastQCFastQC Report
Thu 26 May 2016
SRR1546227_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546227_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences844016
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150231.77994256033061No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10850.1285520653636898No Hit
GGCTAAGGGATTAATCTATTTTGTTGATTTTCTCAAAGAACTGTTTATTA9320.11042444692991601No Hit
ATAACAGGGACATATTTAATTTCTACTCTATCCATGCATTTTCTTCTTTT8910.10556671911432958No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAA351.4454781E-744.015
AATCCGG302.5269146E-644.02
TCGCATC207.855292E-444.028
TCGTTAT207.855292E-444.022
TCGTTAG351.4454781E-744.01
ATTAGCG254.4418317E-544.01
CAACGTA207.855292E-444.040
TACCACG600.044.039
CGTGCGG502.7284841E-1144.02
TCTTACG207.855292E-444.01
TTCACGG254.4418317E-544.02
GAATCGG408.307325E-944.02
TTCGTTA207.855292E-444.021
TACGGGA2850.044.04
ACGGGTA551.8189894E-1244.05
TTATCCG207.855292E-444.01
TCTAGCG207.855292E-444.01
TCTAGAC207.855292E-444.043
CGTTATG207.855292E-444.023
GTTACAT254.4418317E-544.016
Produced by FastQC (version 0.11.5)