FastQCFastQC Report
Thu 26 May 2016
SRR1546226_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546226_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences858891
Sequences flagged as poor quality0
Sequence length50
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151871.7682104015527No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10160.11829207664301988No Hit
GGCTAAGGGATTAATCTATTTTGTTGATTTTCTCAAAGAACTGTTTATTA9590.11165561171324416No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGC8970.1044370007369969No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8870.10327270864405379No Hit
ATAACAGGGACATATTTAATTTCTACTCTATCCATGCATTTTCTTCTTTT8860.10315627943475948No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGA207.855368E-444.00000415
TACCAAC207.855368E-444.00000444
ATAGACG408.309144E-944.0000041
TACGGAC408.309144E-944.0000044
ATGTCGA207.855368E-444.00000437
TCGATAT207.855368E-444.00000430
TCGATAG207.855368E-444.0000041
ACGATAT207.855368E-444.00000425
CCGTACA302.5269655E-644.00000441
ATGCGAG207.855368E-444.0000041
CGGTCTA302.5269655E-644.00000431
ACGTAAC408.309144E-944.00000416
CACGTAT408.309144E-944.00000444
CGACTCA207.855368E-444.00000421
ATACGAG207.855368E-444.0000041
GACGACT207.855368E-444.00000419
GATTCGC408.309144E-944.0000049
TGTACGA207.855368E-444.00000422
TGGTCGA302.5269655E-644.0000046
AATGTCG207.855368E-444.00000436
Produced by FastQC (version 0.11.5)