##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546226_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	858891
Sequences flagged as poor quality	0
Sequence length	50
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96282182488814	33.0	31.0	34.0	30.0	34.0
2	32.352689689378515	33.0	31.0	34.0	31.0	34.0
3	32.37798859226607	34.0	31.0	34.0	31.0	34.0
4	36.07505026831111	37.0	35.0	37.0	35.0	37.0
5	36.001128199038064	37.0	35.0	37.0	35.0	37.0
6	36.07909967621037	37.0	35.0	37.0	35.0	37.0
7	36.25642136196561	37.0	36.0	37.0	35.0	37.0
8	36.17841844890679	37.0	37.0	37.0	35.0	37.0
9	37.95173543557914	39.0	39.0	39.0	35.0	39.0
10	37.61248982699784	39.0	37.0	39.0	35.0	39.0
11	37.45097806357268	39.0	37.0	39.0	35.0	39.0
12	37.09142370801417	39.0	35.0	39.0	34.0	39.0
13	36.96151548915986	39.0	35.0	39.0	33.0	39.0
14	38.12283747297387	40.0	37.0	41.0	33.0	41.0
15	38.271054184989715	40.0	37.0	41.0	33.0	41.0
16	38.35440934879979	40.0	37.0	41.0	34.0	41.0
17	38.28292297858518	40.0	36.0	41.0	34.0	41.0
18	38.086700174993105	39.0	36.0	41.0	34.0	41.0
19	37.855971246642476	39.0	36.0	41.0	34.0	41.0
20	37.57302148933916	39.0	35.0	41.0	34.0	41.0
21	37.46858914577054	39.0	35.0	41.0	33.0	41.0
22	37.41486754431005	39.0	35.0	41.0	33.0	41.0
23	37.34021546389472	39.0	35.0	41.0	33.0	41.0
24	37.29329332825702	39.0	35.0	41.0	33.0	41.0
25	37.272573586171006	39.0	35.0	41.0	33.0	41.0
26	37.23633383048606	39.0	35.0	41.0	33.0	41.0
27	37.162253417488365	39.0	35.0	41.0	33.0	41.0
28	37.08416783969095	39.0	35.0	41.0	33.0	41.0
29	36.9569887215025	39.0	35.0	41.0	33.0	41.0
30	36.74681653434487	39.0	35.0	41.0	32.0	41.0
31	36.42307929644157	39.0	35.0	41.0	31.0	41.0
32	36.069384822986855	39.0	35.0	41.0	30.0	41.0
33	35.62315939973757	39.0	35.0	41.0	27.0	41.0
34	35.195765236799545	39.0	35.0	41.0	23.0	41.0
35	34.90434641881217	38.0	35.0	41.0	21.0	41.0
36	34.70077809640571	38.0	35.0	41.0	20.0	41.0
37	34.58797099981255	38.0	34.0	41.0	18.0	41.0
38	34.526436998408414	38.0	34.0	41.0	18.0	41.0
39	34.43297577923159	38.0	34.0	41.0	18.0	41.0
40	34.33527304396018	38.0	34.0	41.0	18.0	41.0
41	34.2388347299017	38.0	34.0	41.0	18.0	41.0
42	34.16809350662657	38.0	34.0	41.0	17.0	41.0
43	34.08085426439443	38.0	34.0	41.0	16.0	41.0
44	33.93578463390582	38.0	34.0	40.0	15.0	41.0
45	33.8848224047056	38.0	34.0	40.0	15.0	41.0
46	33.81785115922742	37.0	34.0	40.0	15.0	41.0
47	33.79466311790437	37.0	33.0	40.0	17.0	41.0
48	33.74792377612526	37.0	33.0	40.0	17.0	41.0
49	33.631053300127725	37.0	33.0	40.0	16.0	41.0
50	33.52466727442714	37.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	6.0
14	10.0
15	13.0
16	45.0
17	89.0
18	219.0
19	501.0
20	867.0
21	1603.0
22	2570.0
23	4073.0
24	6741.0
25	11929.0
26	19453.0
27	24454.0
28	23826.0
29	20674.0
30	18607.0
31	19276.0
32	21996.0
33	28788.0
34	53769.0
35	92193.0
36	49715.0
37	64171.0
38	114332.0
39	278968.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.46871954648494	21.61845915255836	26.43245766925023	20.48036363170647
2	39.08621699377453	22.20677594712251	27.85580475287318	10.851202306229778
3	25.99247168732703	23.51392667986974	39.84091112842025	10.652690504382978
4	22.925377026887	25.24243472105308	39.77221789493661	12.059970357123314
5	20.524024585191835	29.108583044879964	38.82343626839727	11.543956101530927
6	18.178907451585825	38.11310166249268	35.0080510798227	8.699939806098794
7	78.05006688858074	3.424765191392156	17.182389849235815	1.3427780707912877
8	78.75749076425296	2.58007127796193	17.19216990281654	1.4702680549685583
9	74.8540850934519	4.058023660743912	18.47987695761162	2.6080142881925648
10	36.42802171637612	28.483008903341634	26.057439186113257	9.031530194168992
11	30.265074380800357	21.34764481173979	35.74877370935311	12.638507098106743
12	29.872242228641355	20.24203304028101	37.277605656596705	12.60811907448093
13	19.423419269732715	28.33805453777022	36.84623543616129	15.392290756335786
14	14.610352186715195	30.18427250955011	38.89154735583445	16.313827947900258
15	13.232412494717025	24.623613473653815	48.24488788449291	13.89908614713625
16	16.17958506958392	21.125148592778363	44.17801560384263	18.51725073379509
17	15.530725086186722	23.355466526020184	38.544122595300216	22.569685792492876
18	17.284032548949753	25.343611703929835	41.78399820233301	15.588357544787407
19	19.27555417392894	25.329058052768044	37.20041309083458	18.19497468246844
20	20.560234069282366	23.381779527320695	40.26843918494896	15.789547218447975
21	18.610277672021247	25.928435622215158	39.84114398683884	15.620142718924754
22	20.549639011236582	21.582948243723592	37.42325859742389	20.444154147615937
23	16.138136271075144	25.555396435636187	37.511279079650386	20.795188213638287
24	14.759847291449091	23.813499035384	45.76517858494268	15.661475088224233
25	15.754502026450387	26.32790423930394	40.816238614678696	17.10135511956698
26	15.456093963029069	25.73702600213531	38.83356560960588	19.973314425229745
27	14.012371767779614	29.783988887996266	39.4715976765387	16.732041667685422
28	13.884648925183754	26.25338954535558	42.231784941278924	17.630176588181737
29	14.69639337238369	24.16185522959258	39.982139759294256	21.159611638729476
30	16.61235244053087	28.924275606567072	38.29845696368922	16.164914989212832
31	18.872476251352037	30.225372020430996	36.08211053556272	14.820041192654248
32	20.142718924752966	25.263624837144643	35.75541017428288	18.838246063819508
33	20.21502146372473	29.51946172447959	33.957510324360136	16.30800648743554
34	17.12871598375114	26.431642664785173	36.567503909110705	19.872137442352987
35	19.830805073053508	27.12812219478374	34.61999252524476	18.421080206917992
36	22.501574705055706	29.799124685204525	32.65070887924079	15.04859173049898
37	17.818093331982755	30.60679411007916	35.94670336515344	15.628409192784648
38	17.806799698681207	28.865013139036265	36.11855287807184	17.209634284210686
39	17.06991923305751	29.753251576742567	35.17419556148568	18.002633628714236
40	18.775840007637758	27.105069211343462	34.91059983164336	19.208490949375413
41	15.764980655286875	27.94871526189004	35.4603785579311	20.825925524891982
42	16.936607788415525	28.060370873603286	33.023398778191876	21.97962255978931
43	17.449944172194144	28.40430275785868	33.6900724306111	20.455680639336073
44	16.85813450135116	27.450165387691804	34.386551960609665	21.305148150347367
45	16.4480708262166	33.88427635171401	31.341113133098382	18.32653968897101
46	18.99135047404153	30.737893399744554	33.68285381963486	16.587902306579068
47	17.342945728852673	30.011957279794526	35.05974564874938	17.585351342603428
48	17.529698180560747	27.50977714285049	36.39053151098335	18.569993165605414
49	18.252723570278416	28.30417363786557	36.02971739138028	17.413385400475732
50	17.003554583759755	28.661494881189814	35.8757979766932	18.459152558357232
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1400.0
1	3430.5
2	5461.0
3	17293.0
4	29125.0
5	20647.5
6	12170.0
7	12001.5
8	11833.0
9	12096.0
10	12359.0
11	11907.5
12	11456.0
13	11218.5
14	10981.0
15	10003.0
16	9025.0
17	8648.5
18	8272.0
19	7925.5
20	7579.0
21	8268.0
22	8957.0
23	10047.0
24	11137.0
25	11564.0
26	11991.0
27	16662.5
28	21334.0
29	22648.5
30	23963.0
31	31741.0
32	39519.0
33	44804.0
34	50089.0
35	52361.0
36	54633.0
37	54576.0
38	54519.0
39	54296.5
40	54074.0
41	57061.0
42	60048.0
43	54901.5
44	49755.0
45	46744.5
46	43734.0
47	43376.5
48	43019.0
49	41991.0
50	40963.0
51	36502.5
52	32042.0
53	28078.5
54	24115.0
55	22724.0
56	21333.0
57	20474.5
58	19616.0
59	18698.5
60	17781.0
61	16783.5
62	15786.0
63	14262.0
64	12738.0
65	11149.0
66	9560.0
67	8050.5
68	6541.0
69	5478.0
70	4415.0
71	3751.0
72	3087.0
73	2710.0
74	2333.0
75	1685.5
76	1038.0
77	787.5
78	537.0
79	411.5
80	286.0
81	217.5
82	149.0
83	118.5
84	88.0
85	53.5
86	19.0
87	16.0
88	13.0
89	11.0
90	9.0
91	7.0
92	5.0
93	4.0
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	858891.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.217976128495135
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.21176893179822	21.287660597530614
2	7.86088262549294	4.27914631299138
3	3.059009858749618	2.497801719368352
4	1.710966966289467	1.862762321804418
5	1.1405108410677176	1.5521198423235523
6	0.8382422131072653	1.3689153927750286
7	0.6620446122688698	1.2613660116913041
8	0.5299751085880482	1.153987988339689
9	0.448962241593324	1.0997859216854482
>10	4.655803829776375	26.36650614995893
>50	0.43232989323957643	8.230574358466072
>100	0.4331885424363515	24.565871883236678
>500	0.015455686350553674	2.580124679785118
>1k	4.293246208487132E-4	0.11872312838141667
>5k	0.0	0.0
>10k+	4.293246208487132E-4	1.7746536916619833
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15187	1.7682104015527	No Hit
TGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1016	0.11829207664301988	No Hit
GGCTAAGGGATTAATCTATTTTGTTGATTTTCTCAAAGAACTGTTTATTA	959	0.11165561171324416	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGAGTCGATCGTATGC	897	0.1044370007369969	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	887	0.10327270864405379	No Hit
ATAACAGGGACATATTTAATTTCTACTCTATCCATGCATTTTCTTCTTTT	886	0.10315627943475948	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04226380297383486	0.0
2	0.0	0.0	0.0	0.15927515831461733	0.0
3	0.0	0.0	0.0	0.22831767942614373	0.0
4	0.0	0.0	0.0	0.3251867815590104	0.0
5	0.0	0.0	0.0	0.5685238289841202	0.0
6	0.0	0.0	0.0	0.7681999229238634	0.0
7	0.0	0.0	0.0	0.9004635046822006	0.0
8	0.0	0.0	0.0	1.215520945032606	0.0
9	0.0	0.0	0.0	1.3568660051158994	0.0
10	0.0	0.0	0.0	1.6569040774673387	0.0
11	0.0	0.0	0.0	2.1120258565988	0.0
12	0.0	0.0	0.0	2.415556805229069	0.0
13	0.0	0.0	0.0	2.5282602798259615	0.0
14	0.0	0.0	0.0	2.5821670037292277	0.0
15	0.0	0.0	0.0	2.656099551631115	0.0
16	0.0	0.0	0.0	2.829579073479638	0.0
17	0.0	0.0	0.0	3.02192012723384	0.0
18	0.0	0.0	0.0	3.288310158099223	0.0
19	0.0	0.0	0.0	3.4486331792974894	0.0
20	0.0	0.0	0.0	3.6152433777976483	0.0
21	0.0	0.0	0.0	3.8287745476434147	0.0
22	0.0	0.0	0.0	4.060002957301916	0.0
23	0.0	0.0	0.0	4.3008949913318455	0.0
24	0.0	0.0	0.0	4.503831103131829	0.0
25	0.0	0.0	0.0	4.671372735306343	0.0
26	0.0	0.0	0.0	4.853235160224056	0.0
27	0.0	0.0	0.0	5.078642109417842	0.0
28	0.0	0.0	0.0	5.26411383982368	0.0
29	1.1642920929431092E-4	0.0	0.0	5.486377200366519	0.0
30	1.1642920929431092E-4	0.0	0.0	5.738795726116585	0.0
31	1.1642920929431092E-4	0.0	0.0	5.95686763512483	0.0
32	1.1642920929431092E-4	0.0	0.0	6.145017237344436	0.0
33	1.1642920929431092E-4	0.0	0.0	6.367746314724453	0.0
34	1.1642920929431092E-4	0.0	0.0	6.599207582801543	0.0
35	1.1642920929431092E-4	0.0	0.0	6.872234078596702	0.0
36	1.1642920929431092E-4	0.0	0.0	7.09391529309307	0.0
37	1.1642920929431092E-4	0.0	0.0	7.309192901078251	0.0
38	1.1642920929431092E-4	0.0	0.0	7.56522073231644	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGA	20	7.855368E-4	44.000004	15
TACCAAC	20	7.855368E-4	44.000004	44
ATAGACG	40	8.309144E-9	44.000004	1
TACGGAC	40	8.309144E-9	44.000004	4
ATGTCGA	20	7.855368E-4	44.000004	37
TCGATAT	20	7.855368E-4	44.000004	30
TCGATAG	20	7.855368E-4	44.000004	1
ACGATAT	20	7.855368E-4	44.000004	25
CCGTACA	30	2.5269655E-6	44.000004	41
ATGCGAG	20	7.855368E-4	44.000004	1
CGGTCTA	30	2.5269655E-6	44.000004	31
ACGTAAC	40	8.309144E-9	44.000004	16
CACGTAT	40	8.309144E-9	44.000004	44
CGACTCA	20	7.855368E-4	44.000004	21
ATACGAG	20	7.855368E-4	44.000004	1
GACGACT	20	7.855368E-4	44.000004	19
GATTCGC	40	8.309144E-9	44.000004	9
TGTACGA	20	7.855368E-4	44.000004	22
TGGTCGA	30	2.5269655E-6	44.000004	6
AATGTCG	20	7.855368E-4	44.000004	36
>>END_MODULE