FastQCFastQC Report
Thu 26 May 2016
SRR1546225_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546225_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1409216
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224691.594432649075798No Hit
TTTTGAGGGAGTGTTCTGCTGGTCTCCAATTACCAAGAATTCTCCAAAAA38120.27050501839320584No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37650.26716983060084476No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC30950.2196256641991008No Hit
TTTTATGGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTTTTTG28130.1996145374449339No Hit
ATTGTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT22280.1581020936463963No Hit
TTTATAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT21460.15228325537036197No Hit
ATGGAGGGCAATGTTTTTCATTATATAATTATTCTATTTTTGTAAATTAT21020.1491609519051728No Hit
TCTTGAGGGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT21000.1490190290203915No Hit
AGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGG19860.14092942458785596No Hit
GGTTACGGGATAAGGGCAAACTTTTTTACAATTTCCACATCCTAAGCATT19810.14057461737590263No Hit
AGTTGAGGGCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCC19710.139865002951996No Hit
TTTTTGGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC19570.13887154275852673No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA19400.13766519823788548No Hit
AGATAAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC18940.13440097188791497No Hit
ATAGAGGGCGATTTAAAGGGGCACCATCATGCTATTAATGATATTAAAAT18900.13411712611835233No Hit
AGCGATGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT18380.13042713111403786No Hit
GTTCGCGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA18000.1277305963031927No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGC17940.1273048276488487No Hit
TTGATAGGGGATATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACC17700.12560175303147283No Hit
TGGATTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA17640.12517598437712885No Hit
AATTGAGGGTGATTATCAGGATCTCCTAATATTTATCTCAATTCTTTTGT17600.12489213860756619No Hit
GAAATTGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAACA17450.12382771697170625No Hit
GTTAGCGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC17260.12247944956628368No Hit
ATAACAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT17150.12169887369998637No Hit
ATGGGAGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTTTTTGC16950.12027964485217311No Hit
GACTTTGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC16900.1199248376402198No Hit
GCGCGAGGGATATTCGATAAATCCATAAATAAAAATATATCAACAATAAT16880.11978291475543848No Hit
GAGAATGGGATTTTTAAGTTTCCTTTCATTTAAATACAGATTAGATAACT16370.11616388119351469No Hit
TGTAAAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC16280.11552522821199873No Hit
AGACAAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC16210.11502849811526408No Hit
AAGATAGGGAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAA16140.11453176801852945No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTG16130.1144608065761388No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCC16070.11403503792179481No Hit
AGACAGGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC16030.11375119215223217No Hit
GATTTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT15990.1134673463826695No Hit
AAGCTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT15940.11311253917071619No Hit
TAAAGAGGGTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTC15910.11289965484354422No Hit
ATTTATGGGATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTAT15390.10920965983922976No Hit
GAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCT15170.10764850810663518No Hit
TAAATAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA15000.10644216358599393No Hit
ATACCTGGGGGTGAAGCTTATGAATTTGTGGAGTGAGAGGATTAAAGATA14940.10601639493164994No Hit
TAAGCAGGGAGGAGGTTTCCACCTATGGTTTTCAAAACAATCACCATCAT14910.10580351060447794No Hit
GCGTATGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA14820.10516485762296199No Hit
GGTATAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC14700.10431332031427404No Hit
TTTGAAGGGATTTTAAGACTTCTAAATCAGAGAAGCACTTCAAATTATTT14610.10367466733275808No Hit
TAGAAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA14560.10331986012080477No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATCC1200.044.00000417
CCGGGAC302.528126E-644.0000045
ATAGGTT302.528126E-644.00000428
CGAGTGG302.528126E-644.0000042
TAACGAG302.528126E-644.0000041
GTTACGT302.528126E-644.00000438
TCGGACG302.528126E-644.0000041
AGGCACG302.528126E-644.0000041
TTACGTG302.528126E-644.0000041
GCTAACA302.528126E-644.00000410
ATGCCCG302.528126E-644.00000413
CTTAGTA302.528126E-644.00000411
GTTATAC302.528126E-644.00000417
AATGTCG302.528126E-644.00000413
CGCTTAA302.528126E-644.00000418
TCTGCGA207.8570895E-444.020
GTGCACG254.4433546E-544.01
CATGGGT502.7284841E-1144.04
CTAGACG551.8189894E-1244.01
AGCAACG207.8570895E-444.01
Produced by FastQC (version 0.11.5)