##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR1546225_1.fastq.gz
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	1409216
Sequences flagged as poor quality	0
Sequence length	50
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.980950400790228	33.0	31.0	34.0	30.0	34.0
2	32.312651147872295	33.0	31.0	34.0	30.0	34.0
3	32.133821216903584	33.0	31.0	34.0	30.0	34.0
4	35.927588815341295	37.0	35.0	37.0	35.0	37.0
5	35.9255110643081	37.0	35.0	37.0	35.0	37.0
6	35.99928541827513	37.0	35.0	37.0	35.0	37.0
7	36.22486616671965	37.0	35.0	37.0	35.0	37.0
8	36.12555349925065	37.0	36.0	37.0	35.0	37.0
9	37.93738859053545	39.0	39.0	39.0	35.0	39.0
10	37.62306275262274	39.0	38.0	39.0	35.0	39.0
11	37.40924386325446	39.0	37.0	39.0	35.0	39.0
12	36.3696807302784	38.0	35.0	39.0	33.0	39.0
13	36.082682143830326	38.0	35.0	39.0	33.0	39.0
14	37.17750791929697	39.0	35.0	41.0	33.0	41.0
15	37.465048651164906	39.0	35.0	41.0	33.0	41.0
16	37.623683665243654	39.0	35.0	41.0	34.0	41.0
17	37.53670977337754	39.0	35.0	41.0	34.0	41.0
18	37.30092193105954	39.0	35.0	40.0	34.0	41.0
19	36.929560833825334	37.0	35.0	40.0	34.0	41.0
20	36.489329527907714	36.0	35.0	40.0	33.0	41.0
21	36.38619558676597	35.0	35.0	40.0	33.0	41.0
22	36.31625811798901	35.0	35.0	40.0	33.0	41.0
23	36.27270908079386	35.0	35.0	40.0	33.0	41.0
24	36.22343629365548	35.0	35.0	40.0	33.0	41.0
25	36.213100759571276	35.0	35.0	40.0	33.0	41.0
26	36.14931209977747	35.0	35.0	40.0	33.0	41.0
27	36.04774356805486	35.0	35.0	40.0	33.0	41.0
28	35.98366893364821	35.0	35.0	40.0	32.0	41.0
29	35.87099493619147	35.0	35.0	40.0	32.0	41.0
30	35.599903776284116	35.0	35.0	40.0	31.0	41.0
31	35.11028259684818	35.0	35.0	40.0	30.0	41.0
32	34.53403523661383	35.0	34.0	40.0	24.0	41.0
33	33.88265248194741	35.0	34.0	40.0	21.0	41.0
34	33.23873629024933	35.0	34.0	40.0	15.0	41.0
35	32.87878295449384	35.0	33.0	40.0	12.0	41.0
36	32.66126484513375	35.0	33.0	40.0	10.0	41.0
37	32.57634670625369	35.0	33.0	40.0	10.0	41.0
38	32.56938255029747	35.0	33.0	40.0	10.0	41.0
39	32.53076959103502	35.0	33.0	40.0	10.0	41.0
40	32.30502279281529	35.0	32.0	40.0	10.0	41.0
41	32.19959679708434	35.0	32.0	40.0	10.0	41.0
42	32.08941141741224	35.0	32.0	40.0	10.0	41.0
43	31.915231589763387	35.0	32.0	40.0	9.0	41.0
44	31.7236328568509	35.0	31.0	40.0	8.0	41.0
45	31.686690329942323	35.0	31.0	40.0	8.0	41.0
46	31.641292037558472	35.0	31.0	40.0	8.0	41.0
47	31.614372814387576	35.0	31.0	40.0	8.0	41.0
48	31.56442305508879	35.0	31.0	40.0	8.0	41.0
49	31.52084208524456	35.0	31.0	40.0	8.0	41.0
50	31.289619902129978	35.0	30.0	39.0	8.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	1.0
13	2.0
14	7.0
15	27.0
16	107.0
17	226.0
18	580.0
19	1271.0
20	2389.0
21	3994.0
22	6800.0
23	10917.0
24	17486.0
25	30461.0
26	47368.0
27	56440.0
28	51189.0
29	40916.0
30	36808.0
31	36927.0
32	43025.0
33	55781.0
34	114905.0
35	271081.0
36	63776.0
37	72979.0
38	124631.0
39	319119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.118778100731188	22.09448374131432	27.984070575412147	23.80266758254235
2	37.81045631045915	22.909830714383034	30.106385394432085	9.173327580725738
3	22.84859098959989	23.535994482038237	44.62949611698987	8.985918411371998
4	20.551923906626097	24.853819428675237	44.7030121713066	9.89124449339207
5	18.478572482855714	28.62946489395522	42.601701939234296	10.290260683954767
6	14.192146555247739	38.73671601798447	39.11621781188973	7.95491961487806
7	73.64960375130569	3.8923770380126252	21.023391729869658	1.4346274808120258
8	74.08225566556156	3.5396986693310324	20.21641820700304	2.1616274581043644
9	71.93020800217994	3.322556655615605	21.843209273808984	2.904026068395477
10	47.49158397293247	18.304716948998593	26.083368340978247	8.120330737090695
11	42.53741087242835	15.685884917571189	33.018572028702486	8.75813218129797
12	36.99603183614151	17.80011013215859	36.33027158363232	8.873586448067577
13	14.917656342249874	40.88514464780417	34.73931604523366	9.457882964712294
14	8.502245220037242	41.40358894591035	40.293539102593215	9.800626731459195
15	7.006378014442073	20.118136609291977	63.03859734774513	9.836888028520823
16	8.634517348653436	16.20092306644262	59.0474419819247	16.117117602979246
17	8.442566646986693	17.42770448249239	40.516358031699895	33.61337083882102
18	13.404119737499432	22.192907261910168	49.06550876515736	15.337464235433035
19	19.808815681911078	20.670855284072847	38.997215473000594	20.52311356101549
20	20.234655297697444	19.490198805577002	42.86227235569281	17.412873541032745
21	14.744013692719923	25.421865775012485	43.93357725146465	15.900543280802943
22	18.27526795040647	21.5785940551342	36.157622394295835	23.988515600163495
23	10.635913869839683	26.2478569644398	36.45090603569645	26.66532313002407
24	10.970922839366002	19.724158681139016	56.784481584086464	12.52043689540851
25	9.575678959080793	22.272029270175757	51.15525228211999	16.99703948862346
26	9.201570234797222	28.77209739316045	43.07629206594305	18.95004030609928
27	10.211706367228302	36.56032857986284	37.611835233207685	15.616129819701166
28	9.13394398019892	30.506253122303466	44.359558790135786	16.000244107361823
29	9.479313320314274	23.02698805577002	44.36459705254553	23.12910157137018
30	11.94742324810391	32.47834256778237	40.40260683954766	15.171627344566058
31	18.918888232889778	30.67052886143785	36.1114974567419	14.299085448930468
32	21.20455629229302	24.975589263817614	40.05305077433126	13.766803669558108
33	22.269545619692085	28.473491643580545	33.17078432263045	16.086178414096917
34	16.0855397611154	27.687167900449612	33.55915629683455	22.668136041600437
35	15.419069894182297	24.543150233888912	37.22147633861665	22.81630353331214
36	27.28694536536627	23.969214087833237	33.65786366319997	15.085976883600527
37	15.776786525273629	30.486525841318862	38.46429504064671	15.272392592760797
38	16.058858258776514	29.812463100049957	32.979543235387624	21.149135405785913
39	17.34822766701485	29.70658862800309	34.89074776329534	18.054435941686727
40	18.90178652527363	23.240085267269176	32.804481357009855	25.053646850447343
41	11.97311129024933	25.791078159771107	36.80188132976066	25.433929220218904
42	17.396765293610063	24.520584495208684	35.13960954175939	22.943040669421862
43	18.272358531268452	22.045520346064762	37.169674485671464	22.512446636995325
44	13.374457854580136	27.984070575412147	34.67495401698533	23.96651755302239
45	14.443704868522639	37.58898564875789	29.089436963531494	18.877872519187974
46	22.071988964076482	29.89328818293292	31.554992279395066	16.479730573595532
47	17.513638789227485	29.024436282301647	33.18880852899768	20.27311639947318
48	17.6928873927063	29.09979733412053	34.40828091648122	18.799034356691948
49	21.56759503156365	24.85119385530678	34.0072068440892	19.574004269040373
50	19.05939188882329	28.718521504155504	31.85955879013579	20.362527816885418
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4774.0
1	5455.0
2	6136.0
3	21530.5
4	36925.0
5	28914.5
6	20904.0
7	23932.0
8	26960.0
9	30275.0
10	33590.0
11	33238.5
12	32887.0
13	30786.0
14	28685.0
15	25849.0
16	23013.0
17	20106.0
18	17199.0
19	16326.0
20	15453.0
21	13882.0
22	12311.0
23	13431.0
24	14551.0
25	13314.0
26	12077.0
27	15814.0
28	19551.0
29	24397.0
30	29243.0
31	38835.5
32	48428.0
33	53685.0
34	58942.0
35	56695.5
36	54449.0
37	57076.0
38	59703.0
39	82338.5
40	104974.0
41	115903.5
42	126833.0
43	138838.5
44	150844.0
45	139271.0
46	127698.0
47	113408.5
48	99119.0
49	88882.0
50	78645.0
51	63874.0
52	49103.0
53	40174.5
54	31246.0
55	25659.5
56	20073.0
57	18502.0
58	16931.0
59	15182.5
60	13434.0
61	11974.0
62	10514.0
63	9719.0
64	8924.0
65	7014.5
66	5105.0
67	4056.0
68	3007.0
69	3092.5
70	3178.0
71	2423.0
72	1668.0
73	1371.0
74	1074.0
75	794.0
76	514.0
77	374.5
78	235.0
79	230.5
80	226.0
81	140.0
82	54.0
83	34.0
84	14.0
85	13.5
86	13.0
87	9.5
88	6.0
89	3.5
90	1.0
91	0.5
92	0.0
93	1.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1409216.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	23.159197249125604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.37835135880563	18.614980936781027
2	8.353748206849577	3.8693220498391723
3	3.3382914264825154	2.319364488629204
4	1.6744757575469944	1.5511805743163947
5	1.086193309340298	1.2577682550846232
6	0.6686478965729831	0.9291209116167992
7	0.4834767663151661	0.7837853657523637
8	0.378242113959831	0.7007826980097576
9	0.2666324162500382	0.5557493448851037
>10	2.1505537449840215	10.676643261290318
>50	0.5795420014677563	9.842933215555384
>100	0.5345109983037128	23.564736285626456
>500	0.07309815729830561	12.179412652324844
>1k	0.033927414779804294	11.549255836112057
>5k	0.0	0.0
>10k+	3.084310434527663E-4	1.6049641241765014
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22469	1.594432649075798	No Hit
TTTTGAGGGAGTGTTCTGCTGGTCTCCAATTACCAAGAATTCTCCAAAAA	3812	0.27050501839320584	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	3765	0.26716983060084476	No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC	3095	0.2196256641991008	No Hit
TTTTATGGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTTTTTG	2813	0.1996145374449339	No Hit
ATTGTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	2228	0.1581020936463963	No Hit
TTTATAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	2146	0.15228325537036197	No Hit
ATGGAGGGCAATGTTTTTCATTATATAATTATTCTATTTTTGTAAATTAT	2102	0.1491609519051728	No Hit
TCTTGAGGGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT	2100	0.1490190290203915	No Hit
AGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGG	1986	0.14092942458785596	No Hit
GGTTACGGGATAAGGGCAAACTTTTTTACAATTTCCACATCCTAAGCATT	1981	0.14057461737590263	No Hit
AGTTGAGGGCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCC	1971	0.139865002951996	No Hit
TTTTTGGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1957	0.13887154275852673	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA	1940	0.13766519823788548	No Hit
AGATAAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1894	0.13440097188791497	No Hit
ATAGAGGGCGATTTAAAGGGGCACCATCATGCTATTAATGATATTAAAAT	1890	0.13411712611835233	No Hit
AGCGATGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1838	0.13042713111403786	No Hit
GTTCGCGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA	1800	0.1277305963031927	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGC	1794	0.1273048276488487	No Hit
TTGATAGGGGATATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACC	1770	0.12560175303147283	No Hit
TGGATTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA	1764	0.12517598437712885	No Hit
AATTGAGGGTGATTATCAGGATCTCCTAATATTTATCTCAATTCTTTTGT	1760	0.12489213860756619	No Hit
GAAATTGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAACA	1745	0.12382771697170625	No Hit
GTTAGCGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1726	0.12247944956628368	No Hit
ATAACAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT	1715	0.12169887369998637	No Hit
ATGGGAGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTTTTTGC	1695	0.12027964485217311	No Hit
GACTTTGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1690	0.1199248376402198	No Hit
GCGCGAGGGATATTCGATAAATCCATAAATAAAAATATATCAACAATAAT	1688	0.11978291475543848	No Hit
GAGAATGGGATTTTTAAGTTTCCTTTCATTTAAATACAGATTAGATAACT	1637	0.11616388119351469	No Hit
TGTAAAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1628	0.11552522821199873	No Hit
AGACAAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1621	0.11502849811526408	No Hit
AAGATAGGGAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAA	1614	0.11453176801852945	No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTG	1613	0.1144608065761388	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCC	1607	0.11403503792179481	No Hit
AGACAGGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1603	0.11375119215223217	No Hit
GATTTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1599	0.1134673463826695	No Hit
AAGCTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT	1594	0.11311253917071619	No Hit
TAAAGAGGGTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTC	1591	0.11289965484354422	No Hit
ATTTATGGGATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTAT	1539	0.10920965983922976	No Hit
GAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCT	1517	0.10764850810663518	No Hit
TAAATAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	1500	0.10644216358599393	No Hit
ATACCTGGGGGTGAAGCTTATGAATTTGTGGAGTGAGAGGATTAAAGATA	1494	0.10601639493164994	No Hit
TAAGCAGGGAGGAGGTTTCCACCTATGGTTTTCAAAACAATCACCATCAT	1491	0.10580351060447794	No Hit
GCGTATGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA	1482	0.10516485762296199	No Hit
GGTATAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC	1470	0.10431332031427404	No Hit
TTTGAAGGGATTTTAAGACTTCTAAATCAGAGAAGCACTTCAAATTATTT	1461	0.10367466733275808	No Hit
TAGAAAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA	1456	0.10331986012080477	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10963542849357373	0.0
2	0.0	0.0	0.0	0.4442895908079386	0.0
3	0.0	0.0	0.0	0.6156614741813888	0.0
4	0.0	0.0	0.0	0.9636563876651982	0.0
5	0.0	0.0	0.0	1.6261524138244243	0.0
6	0.0	0.0	0.0	2.213074503837595	0.0
7	0.0	0.0	0.0	2.579377469458195	0.0
8	0.0	0.0	0.0	3.48590989599891	0.0
9	0.0	0.0	0.0	3.829789045824061	0.0
10	0.0	0.0	0.0	4.5340813615513875	0.0
11	0.0	0.0	0.0	5.162303011035924	0.0
12	0.0	0.0	0.0	5.779525636949907	0.0
13	0.0	0.0	0.0	5.982830169399155	0.0
14	7.096144239066261E-5	0.0	0.0	6.051449884190926	0.0
15	7.096144239066261E-5	0.0	0.0	6.166975112402925	0.0
16	7.096144239066261E-5	0.0	0.0	6.426906875879922	0.0
17	7.096144239066261E-5	0.0	0.0	6.750632976066124	0.0
18	7.096144239066261E-5	0.0	0.0	7.325775466642445	0.0
19	7.096144239066261E-5	0.0	0.0	7.544478632090468	0.0
20	7.096144239066261E-5	0.0	0.0	7.82108633452927	0.0
21	7.096144239066261E-5	0.0	0.0	8.106989985921249	0.0
22	7.096144239066261E-5	0.0	0.0	8.372385780462327	0.0
23	7.096144239066261E-5	0.0	0.0	8.679648826013898	0.0
24	7.096144239066261E-5	0.0	0.0	8.888346428084835	0.0
25	7.096144239066261E-5	0.0	0.0	9.050138516735547	0.0
26	7.096144239066261E-5	0.0	0.0	9.260610154866251	0.0
27	7.096144239066261E-5	0.0	0.0	9.440497411326582	0.0
28	7.096144239066261E-5	0.0	0.0	9.663954993414778	0.0
29	7.096144239066261E-5	0.0	0.0	9.856047617966302	0.0
30	7.096144239066261E-5	0.0	0.0	10.19013408874154	0.0
31	7.096144239066261E-5	0.0	0.0	10.523936713747219	0.0
32	7.096144239066261E-5	0.0	0.0	10.774501566828649	0.0
33	7.096144239066261E-5	0.0	0.0	10.98554089649848	0.0
34	7.096144239066261E-5	0.0	0.0	11.201192719923702	0.0
35	7.096144239066261E-5	0.0	0.0	11.559122235342205	0.0
36	7.096144239066261E-5	0.0	0.0	11.835517053453835	0.0
37	7.096144239066261E-5	0.0	0.0	12.093319973659112	0.0
38	7.096144239066261E-5	0.0	0.0	12.323589854216813	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCC	120	0.0	44.000004	17
CCGGGAC	30	2.528126E-6	44.000004	5
ATAGGTT	30	2.528126E-6	44.000004	28
CGAGTGG	30	2.528126E-6	44.000004	2
TAACGAG	30	2.528126E-6	44.000004	1
GTTACGT	30	2.528126E-6	44.000004	38
TCGGACG	30	2.528126E-6	44.000004	1
AGGCACG	30	2.528126E-6	44.000004	1
TTACGTG	30	2.528126E-6	44.000004	1
GCTAACA	30	2.528126E-6	44.000004	10
ATGCCCG	30	2.528126E-6	44.000004	13
CTTAGTA	30	2.528126E-6	44.000004	11
GTTATAC	30	2.528126E-6	44.000004	17
AATGTCG	30	2.528126E-6	44.000004	13
CGCTTAA	30	2.528126E-6	44.000004	18
TCTGCGA	20	7.8570895E-4	44.0	20
GTGCACG	25	4.4433546E-5	44.0	1
CATGGGT	50	2.7284841E-11	44.0	4
CTAGACG	55	1.8189894E-12	44.0	1
AGCAACG	20	7.8570895E-4	44.0	1
>>END_MODULE