FastQCFastQC Report
Thu 26 May 2016
SRR1546224_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1546224_1.fastq.gz
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences1438977
Sequences flagged as poor quality0
Sequence length50
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT227771.5828606016635427No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA39340.2733886643080466No Hit
TTTTGAGGGAGTGTTCTGCTGGTCTCCAATTACCAAGAATTCTCCAAAAA38630.2684546035134682No Hit
ATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATCCC30600.212651070864927No Hit
TTTTATGGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTTTTTG26570.1846450638196441No Hit
ATTGTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT22990.1597662784047278No Hit
ATGGAGGGCAATGTTTTTCATTATATAATTATTCTATTTTTGTAAATTAT21610.15017613207160366No Hit
TTTATAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT21530.1496201815595385No Hit
TCTTGAGGGGTTTCCTCCTATGGTTTTCAAAACAATCACCATCATGCTAT21510.1494811939315222No Hit
AGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGATGG20690.14378270118285422No Hit
GGTTACGGGATAAGGGCAAACTTTTTTACAATTTCCACATCCTAAGCATT19930.1385011713182351No Hit
AGTTGAGGGCAAAACAATCACCATCATGCTATTAATGATATTAAAATCCC19860.13801471462017809No Hit
TTTTTGGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC19670.1366943321540233No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGC19200.13342812289564046No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATA19190.1333586290816323No Hit
AGATAAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC18840.1309263455913472No Hit
AGCGATGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT18690.129883938381225No Hit
GTTCGCGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAAAA18510.12863304972907835No Hit
ATAGAGGGCGATTTAAAGGGGCACCATCATGCTATTAATGATATTAAAAT18480.12842456828705393No Hit
TTGATAGGGGATATAGGAGGTTTCCTCCTATGGTTTTCAAAACAATCACC18340.1274516548909399No Hit
TGGATTAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAA18300.1271736796349073No Hit
GTTAGCGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC18270.12696519819288285No Hit
GAAATTGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAACA18020.1252278528426792No Hit
AATTGAGGGTGATTATCAGGATCTCCTAATATTTATCTCAATTCTTTTGT17830.12390747037652444No Hit
GAGAATGGGATTTTTAAGTTTCCTTTCATTTAAATACAGATTAGATAACT17750.12335151986445926No Hit
GACTTTGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC17480.12147518688623933No Hit
GCGCGAGGGATATTCGATAAATCCATAAATAAAAATATATCAACAATAAT17160.11925138483797865No Hit
ATAACAGGGGGGAATTCAATACCTTTACAAATGCTTTAACAAGAGGAAAT16890.1173750518597587No Hit
AGACAAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC16740.11633264464963652No Hit
ATGGGAGGGATTTCTAAAAATAATGTTAAGAATTTTTGCTGGTTTTTTGC16610.11542922506753062No Hit
TAAAGAGGGTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTC16510.11473428692744915No Hit
TGTAAAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC16450.11431732404340027No Hit
AAGATAGGGAACTATACCAAAGAATATCCCAATTATCCATAAAACTGTAA16210.11264947250720478No Hit
CCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTG16110.11195453436712331No Hit
AGACAGGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC15890.1104256704589441No Hit
AAGCTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT15840.11007820138890335No Hit
ATTTATGGGATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTAT15780.1096612385048545No Hit
GATTTAGGGATGCCACAACTAGATACATCAACATGATTTATCACAATTAT15690.10903579417878118No Hit
TAAATAGGGACTAATCCTAGCCCTAGCCCTACACAAATATAATTATACTA15580.10827136222469158No Hit
GAAGATGGGAAGCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCT15550.10806288078266714No Hit
TAAGCAGGGAGGAGGTTTCCACCTATGGTTTTCAAAACAATCACCATCAT15420.10715946120056123No Hit
GGTATAGGGGGGAATTCATCGACAATAAACAAAGAAATAGAACTTTTAAC15330.10653401687448791No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCC15320.10646452306047978No Hit
CTGTCTCTTATACACATCTGACGCTTAGCCAGTCGTATGCCGTCTTCTGC15210.10570009110639016No Hit
TTTGAAGGGATTTTAAGACTTCTAAATCAGAGAAGCACTTCAAATTATTT15180.10549160966436572No Hit
ATACCTGGGGGTGAAGCTTATGAATTTGTGGAGTGAGAGGATTAAAGATA15070.10472717771027612No Hit
GCGTATGGGGGTTTTCAAAACAATCACCATCATGCTATTAATGATATTAA14910.10361527668614579No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGGC750.044.0000044
TAGCGAT551.8189894E-1244.00000433
ACCTGGT1500.044.00000411
TGACCGG750.044.0000042
GTGCATA207.857144E-444.030
ATTTACG502.7284841E-1144.01
GTGCACG502.7284841E-1144.01
CTAGAGT351.446424E-744.033
CTAGACG600.044.01
AAGTACG302.5281643E-644.01
AGACTCG207.857144E-444.031
AACCGGT254.443401E-544.041
GGTACTC502.7284841E-1144.08
AACCGAA351.446424E-744.010
GTCGCGA207.857144E-444.022
GTACCGG254.443401E-544.02
GTACCAC254.443401E-544.016
TACCCGC600.044.040
CCGGGAT408.314601E-944.05
GGAGCAT207.857144E-444.035
Produced by FastQC (version 0.11.5)